{"id":"https://openalex.org/W2594356239","doi":null,"title":"Exogenously-introduced Homing Endonucleases Catalyze Double-stranded DNA Breaks in Aedes aegypti","display_name":"Exogenously-introduced Homing Endonucleases Catalyze Double-stranded DNA Breaks in Aedes aegypti","publication_year":2009,"publication_date":"2009-01-13","ids":{"openalex":"https://openalex.org/W2594356239","mag":"2594356239"},"language":"en","primary_location":{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/40967","doi":null,"is_oa":false,"landing_page_url":"http://scholar.lib.vt.edu/theses/available/etd-02072009-102134/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079699662","display_name":"Brenna E. Traver","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Brenna E. Traver","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5079699662"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9818999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9818999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.004600000102072954,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11075","display_name":"Insect Resistance and Genetics","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/homing-endonucleases","display_name":"homing endonucleases","score":0.9760000109672546},{"id":"https://openalex.org/keywords/aedes-aegypti","display_name":"Aedes aegypti","score":0.9290000200271606},{"id":"https://openalex.org/keywords/dna-double-strand-breaks","display_name":"DNA double-strand breaks","score":0.9369999766349792},{"id":"https://openalex.org/keywords/selfish-genetic-elements","display_name":"selfish genetic elements","score":0.8360000252723694},{"id":"https://openalex.org/keywords/germline-modification","display_name":"germline modification","score":0.847000002861023},{"id":"https://openalex.org/keywords/i-scei","display_name":"I-SceI","score":0.9110000133514404},{"id":"https://openalex.org/keywords/dengue-vector","display_name":"dengue vector","score":0.5720000267028809}],"concepts":[{"id":"https://openalex.org/C2777775583","wikidata":"https://www.wikidata.org/wiki/Q1148004","display_name":"Aedes aegypti","level":3,"score":0.8914647698402405},{"id":"https://openalex.org/C175817850","wikidata":"https://www.wikidata.org/wiki/Q953859","display_name":"Homing endonuclease","level":4,"score":0.7203861474990845},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6190494298934937},{"id":"https://openalex.org/C151872237","wikidata":"https://www.wikidata.org/wiki/Q1410759","display_name":"Homing (biology)","level":2,"score":0.4792599678039551},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46271321177482605},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3781101107597351},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.37255334854125977},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3490903377532959},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3426613211631775},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.33001917600631714},{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.2096835970878601},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.123771071434021},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.12025931477546692},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/40967","doi":null,"is_oa":false,"landing_page_url":"http://scholar.lib.vt.edu/theses/available/etd-02072009-102134/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:2594356239","doi":null,"is_oa":false,"landing_page_url":"https://vtechworks.lib.vt.edu/handle/10919/40967","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.428600013256073}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":129,"referenced_works":["https://openalex.org/W7357641","https://openalex.org/W35995934","https://openalex.org/W147499388","https://openalex.org/W962995633","https://openalex.org/W1513438412","https://openalex.org/W1534179616","https://openalex.org/W1556733566","https://openalex.org/W1591674227","https://openalex.org/W1602488389","https://openalex.org/W1635062911","https://openalex.org/W1787672602","https://openalex.org/W1832001947","https://openalex.org/W1846243202","https://openalex.org/W1855484651","https://openalex.org/W1868621362","https://openalex.org/W1911019285","https://openalex.org/W1965747854","https://openalex.org/W1967287827","https://openalex.org/W1967976283","https://openalex.org/W1967999689","https://openalex.org/W1969022737","https://openalex.org/W1969673444","https://openalex.org/W1971330622","https://openalex.org/W1972933969","https://openalex.org/W1973403924","https://openalex.org/W1975104335","https://openalex.org/W1976459530","https://openalex.org/W1979128283","https://openalex.org/W1983509233","https://openalex.org/W1983858690","https://openalex.org/W1987226094","https://openalex.org/W1987327670","https://openalex.org/W1988375781","https://openalex.org/W1991200056","https://openalex.org/W1993097572","https://openalex.org/W1997060995","https://openalex.org/W2001488804","https://openalex.org/W2004360848","https://openalex.org/W2006058818","https://openalex.org/W2007111715","https://openalex.org/W2007143460","https://openalex.org/W2011764908","https://openalex.org/W2015159728","https://openalex.org/W2015911939","https://openalex.org/W2018438969","https://openalex.org/W2018750823","https://openalex.org/W2019056007","https://openalex.org/W2020013548","https://openalex.org/W2024329194","https://openalex.org/W2024533323","https://openalex.org/W2025438502","https://openalex.org/W2027166966","https://openalex.org/W2027875921","https://openalex.org/W2036314397","https://openalex.org/W2038740710","https://openalex.org/W2043951241","https://openalex.org/W2046997318","https://openalex.org/W2048052068","https://openalex.org/W2048064261","https://openalex.org/W2048075762","https://openalex.org/W2049361776","https://openalex.org/W2050517636","https://openalex.org/W2050537059","https://openalex.org/W2050585747","https://openalex.org/W2050645140","https://openalex.org/W2050845260","https://openalex.org/W2051395442","https://openalex.org/W2053350421","https://openalex.org/W2056026468","https://openalex.org/W2058304957","https://openalex.org/W2060292520","https://openalex.org/W2061492996","https://openalex.org/W2061636126","https://openalex.org/W2061687100","https://openalex.org/W2065009864","https://openalex.org/W2065613928","https://openalex.org/W2068876203","https://openalex.org/W2071992752","https://openalex.org/W2072749607","https://openalex.org/W2075529916","https://openalex.org/W2077123848","https://openalex.org/W2078308455","https://openalex.org/W2078904081","https://openalex.org/W2081688244","https://openalex.org/W2086060314","https://openalex.org/W2097333494","https://openalex.org/W2097852545","https://openalex.org/W2101353772","https://openalex.org/W2103334250","https://openalex.org/W2103622571","https://openalex.org/W2105980782","https://openalex.org/W2106154620","https://openalex.org/W2107383419","https://openalex.org/W2107817332","https://openalex.org/W2108927019","https://openalex.org/W2110362074","https://openalex.org/W2112291724","https://openalex.org/W2112327838","https://openalex.org/W2113118721","https://openalex.org/W2113446817","https://openalex.org/W2116417286","https://openalex.org/W2118149818","https://openalex.org/W2119889845","https://openalex.org/W2119983182","https://openalex.org/W2121362920","https://openalex.org/W2122265494","https://openalex.org/W2125071927","https://openalex.org/W2129192681","https://openalex.org/W2130883309","https://openalex.org/W2133219008","https://openalex.org/W2133385816","https://openalex.org/W2135547884","https://openalex.org/W2135777982","https://openalex.org/W2139200003","https://openalex.org/W2140192391","https://openalex.org/W2140289804","https://openalex.org/W2141509764","https://openalex.org/W2145094693","https://openalex.org/W2145193899","https://openalex.org/W2151364212","https://openalex.org/W2152331729","https://openalex.org/W2153588135","https://openalex.org/W2156677904","https://openalex.org/W2158159015","https://openalex.org/W2162355280","https://openalex.org/W2164332752","https://openalex.org/W2165011963","https://openalex.org/W2165294470","https://openalex.org/W2165359856"],"related_works":["https://openalex.org/W2171371781","https://openalex.org/W2038417498","https://openalex.org/W2469463309","https://openalex.org/W7124632834","https://openalex.org/W2994146178","https://openalex.org/W2027166966","https://openalex.org/W4378714015","https://openalex.org/W7165737151","https://openalex.org/W4399125233","https://openalex.org/W2155610396"],"abstract_inverted_index":{"Aedes":[0],"aegypti":[1,83,95],"transmits":[2],"the":[3,59],"viruses":[4],"which":[5,21],"cause":[6],"yellow":[7],"fever,":[8,10],"dengue":[9,12],"and":[11,53,99,125,135,161,198],"hemorrhagic":[13],"fever.":[14],"Homing":[15],"endonucleases":[16],"are":[17],"selfish":[18],"genetic":[19],"elements":[20],"introduce":[22],"double-stranded":[23],"DNA":[24,211],"(dsDNA)":[25],"breaks":[26,48,73,208],"in":[27,49,81,123,182,209,212],"a":[28,38,113,147,187,191],"sequence-specific":[29],"manner.":[30],"In":[31,110,139,189],"this":[32],"study,":[33],"we":[34],"aimed":[35],"to":[36,41,66,70,78,133,158,186,200],"validate":[37],"somatic":[39,192],"assay":[40,64,195],"detect":[42,201],"recombinant":[43],"homing":[44],"endonuclease":[45],"(rHE)-induced":[46],"dsDNA":[47,72,207],"both":[50],"cultured":[51],"cells":[52],"adult":[54],"female":[55],"Ae.":[56,82,94,213],"aegypti.":[57,214],"While":[58],"cell":[60],"culture-based":[61],"two":[62,142],"plasmid":[63],"used":[65,77],"test":[67,79],"rHE":[68,202],"ability":[69],"induce":[71],"was":[74,108,121,180,196],"inconclusive,":[75],"assays":[76],"rHEs":[80,90],"were":[84,91],"successful.":[85],"Recognition":[86],"sequences":[87],"for":[88],"various":[89],"introduced":[92],"into":[93],"through":[96],"germline":[97],"transformation,":[98],"imperfect":[100,118,173],"repair":[101,120,174],"at":[102,175],"each":[103],"of":[104,127,152,165,169],"these":[105],"exogenous":[106,115,143],"sites":[107,145],"evaluated.":[109],"mosquitoes":[111,131,140,156,183],"containing":[112,141],"single":[114],"HE":[116,144,177],"site,":[117],"gap":[119],"detected":[122,181],"40%":[124],"21%":[126],"clones":[128,153],"sequenced":[129,154],"from":[130,155],"exposed":[132,157,185],"I-PpoI":[134],"I\u00e2":[136],"SceI,":[137],"respectively.":[138],"flanking":[146],"marker":[148],"gene":[149],"(EGFP),":[150],"100%":[151],"I-PpoI,":[159],"I-CreI,":[160],"I-AniI":[162],"demonstrated":[163],"excision":[164,171],"EGFP.":[166],"No":[167],"evidence":[168],"EGFP":[170],"or":[172,203],"any":[176],"recognition":[178],"site":[179],"not":[184],"rHE.":[188],"summary,":[190],"genomic":[193],"footprint":[194],"developed":[197],"validated":[199],"other":[204],"meganuclease-induced":[205],"site-specific":[206],"chromosomal":[210]},"counts_by_year":[],"updated_date":"2026-08-08T01:25:22.217667","created_date":"2017-03-16T00:00:00"}