{"id":"https://openalex.org/W2593250554","doi":"https://doi.org/10.1515/tjb-2016-0297","title":"Prevention of protein aggregation by extracellular fungal sucrase of Termitomyces clypeatus","display_name":"Prevention of protein aggregation by extracellular fungal sucrase of Termitomyces clypeatus","publication_year":2017,"publication_date":"2017-03-11","ids":{"openalex":"https://openalex.org/W2593250554","doi":"https://doi.org/10.1515/tjb-2016-0297","mag":"2593250554"},"language":"en","primary_location":{"id":"doi:10.1515/tjb-2016-0297","doi":"https://doi.org/10.1515/tjb-2016-0297","is_oa":true,"landing_page_url":"https://doi.org/10.1515/tjb-2016-0297","pdf_url":null,"source":{"id":"https://openalex.org/S4210194879","display_name":"Turkish Journal of Biochemistry","issn_l":"0250-4685","issn":["0250-4685","1303-829X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","poland-20","russia-white-list-4","tr-dizin"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Turkish Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1515/tjb-2016-0297","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111999311","display_name":"Sudeshna Chowdhury","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I160344538","display_name":"Indian Institute of Chemical Biology","ror":"https://ror.org/01kh0x418","country_code":"IN","type":"facility","lineage":["https://openalex.org/I160344538","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sudeshna Chowdhury","raw_affiliation_strings":["Drug Development and Biotechnology Division, Indian Institute of Chemical Biology, (CSIR, Govt. of India) , Kolkata , West Bengal , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Drug Development and Biotechnology Division, Indian Institute of Chemical Biology, (CSIR, Govt. of India) , Kolkata , West Bengal , India","institution_ids":["https://openalex.org/I160344538","https://openalex.org/I66760702"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015593138","display_name":"Samudra Prosad Banik","orcid":"https://orcid.org/0000-0003-0075-7508","observed_orcids":["https://orcid.org/0000-0003-0075-7508"]},"institutions":[{"id":"https://openalex.org/I4405272376","display_name":"Maulana Azad College","ror":"https://ror.org/04334z971","country_code":"IN","type":"education","lineage":["https://openalex.org/I4405272376"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Samudra Prosad Banik","raw_affiliation_strings":["Maulana Azad College, Department of Microbiology , Kolkata , West Bengal , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Maulana Azad College, Department of Microbiology , Kolkata , West Bengal , India","institution_ids":["https://openalex.org/I4405272376"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062220907","display_name":"Rajib Majumder","orcid":"https://orcid.org/0000-0001-9069-2319","observed_orcids":["https://orcid.org/0000-0001-9069-2319"]},"institutions":[{"id":"https://openalex.org/I160344538","display_name":"Indian Institute of Chemical Biology","ror":"https://ror.org/01kh0x418","country_code":"IN","type":"facility","lineage":["https://openalex.org/I160344538","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rajib Majumder","raw_affiliation_strings":["Structural Biology and Bioinformatics Division CSIR-Indian Institute of Chemical Biology Kolkata , West Bengal , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Structural Biology and Bioinformatics Division CSIR-Indian Institute of Chemical Biology Kolkata , West Bengal , India","institution_ids":["https://openalex.org/I160344538"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030185584","display_name":"Shakuntala Ghorai","orcid":"https://orcid.org/0000-0002-3985-9651","observed_orcids":["https://orcid.org/0000-0002-3985-9651"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shakuntala Ghorai","raw_affiliation_strings":["Raidighi College, Department of Microbiology South 24 Parganas , West Bengal , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Raidighi College, Department of Microbiology South 24 Parganas , West Bengal , India","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071885480","display_name":"Swagata Pal","orcid":"https://orcid.org/0000-0002-7965-4589","observed_orcids":["https://orcid.org/0000-0002-7965-4589"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Swagata Pal","raw_affiliation_strings":["Y.S. Palpara Mahavidyalaya Palpara, Purba Medinipore , West Bengal , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Y.S. Palpara Mahavidyalaya Palpara, Purba Medinipore , West Bengal , India","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110496201","display_name":"Pijush Basak","orcid":"https://orcid.org/0009-0008-0422-872X","observed_orcids":["https://orcid.org/0009-0008-0422-872X"]},"institutions":[{"id":"https://openalex.org/I106542073","display_name":"University of Calcutta","ror":"https://ror.org/01e7v7w47","country_code":"IN","type":"education","lineage":["https://openalex.org/I106542073"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Pijush Basak","raw_affiliation_strings":["Department of Biochemistry , University of Calcutta , Kolkata , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry , University of Calcutta , Kolkata , India","institution_ids":["https://openalex.org/I106542073"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5004785943","display_name":"Suman Khowala","orcid":"https://orcid.org/0000-0002-2034-2900","observed_orcids":["https://orcid.org/0000-0002-2034-2900"]},"institutions":[{"id":"https://openalex.org/I160344538","display_name":"Indian Institute of Chemical Biology","ror":"https://ror.org/01kh0x418","country_code":"IN","type":"facility","lineage":["https://openalex.org/I160344538","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Suman Khowala","raw_affiliation_strings":["Drug Development and Biotechnology Division, Indian Institute of Chemical Biology, (CSIR, Govt. of India) , Kolkata , West Bengal , India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Drug Development and Biotechnology Division, Indian Institute of Chemical Biology, (CSIR, Govt. of India) , Kolkata , West Bengal , India","institution_ids":["https://openalex.org/I160344538","https://openalex.org/I66760702"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5004785943"],"corresponding_institution_ids":["https://openalex.org/I160344538","https://openalex.org/I66760702"],"apc_list":{"value":750,"currency":"EUR","value_usd":848},"apc_paid":{"value":750,"currency":"EUR","value_usd":848},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"42","issue":"3","first_page":"355","last_page":"364"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.5820000171661377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.5820000171661377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.15649999678134918,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.07029999792575836,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/termitomyces-clypeatus","display_name":"Termitomyces clypeatus","score":0.9509999752044678},{"id":"https://openalex.org/keywords/protein-aggregation-prevention","display_name":"protein aggregation prevention","score":0.9169999957084656},{"id":"https://openalex.org/keywords/protein-misfolding","display_name":"protein misfolding","score":0.5809999704360962},{"id":"https://openalex.org/keywords/insulin","display_name":"insulin","score":0.5649999976158142},{"id":"https://openalex.org/keywords/carbonic-anhydrase","display_name":"carbonic anhydrase","score":0.9240000247955322},{"id":"https://openalex.org/keywords/alcohol-dehydrogenase","display_name":"alcohol dehydrogenase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/whey-protein","display_name":"whey protein","score":0.9779999852180481},{"id":"https://openalex.org/keywords/bovine-serum-albumin","display_name":"bovine serum albumin","score":0.8790000081062317},{"id":"https://openalex.org/keywords/thermal-aggregation","display_name":"thermal aggregation","score":0.7450000047683716},{"id":"https://openalex.org/keywords/protein-thermostability","display_name":"protein thermal stability","score":0.5820000171661377},{"id":"https://openalex.org/keywords/proteinopathies","display_name":"proteinopathies","score":0.5379999876022339}],"concepts":[{"id":"https://openalex.org/C2776039999","wikidata":"https://www.wikidata.org/wiki/Q2138267","display_name":"Sucrase","level":3,"score":0.8690728545188904},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7439631819725037},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6816489696502686},{"id":"https://openalex.org/C2777824588","wikidata":"https://www.wikidata.org/wiki/Q414783","display_name":"Dithiothreitol","level":3,"score":0.5345451235771179},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5296280980110168},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.508794367313385},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.475925475358963},{"id":"https://openalex.org/C2775914622","wikidata":"https://www.wikidata.org/wiki/Q284089","display_name":"Carbonic anhydrase","level":3,"score":0.4155947268009186},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.35225728154182434}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1515/tjb-2016-0297","doi":"https://doi.org/10.1515/tjb-2016-0297","is_oa":true,"landing_page_url":"https://doi.org/10.1515/tjb-2016-0297","pdf_url":null,"source":{"id":"https://openalex.org/S4210194879","display_name":"Turkish Journal of Biochemistry","issn_l":"0250-4685","issn":["0250-4685","1303-829X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","poland-20","russia-white-list-4","tr-dizin"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Turkish Journal of Biochemistry","raw_type":"journal-article"},{"id":"doi:10.60692/e0psm-hm334","doi":"https://doi.org/10.60692/e0psm-hm334","is_oa":true,"landing_page_url":"https://doi.org/10.60692/e0psm-hm334","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},{"id":"doi:10.60692/zqpp7-ccm70","doi":"https://doi.org/10.60692/zqpp7-ccm70","is_oa":true,"landing_page_url":"https://doi.org/10.60692/zqpp7-ccm70","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.1515/tjb-2016-0297","doi":"https://doi.org/10.1515/tjb-2016-0297","is_oa":true,"landing_page_url":"https://doi.org/10.1515/tjb-2016-0297","pdf_url":null,"source":{"id":"https://openalex.org/S4210194879","display_name":"Turkish Journal of Biochemistry","issn_l":"0250-4685","issn":["0250-4685","1303-829X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","poland-20","russia-white-list-4","tr-dizin"],"host_organization":"https://openalex.org/P4310313990","host_organization_name":"De Gruyter","host_organization_lineage":["https://openalex.org/P4310313990"],"host_organization_lineage_names":["De Gruyter"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Turkish Journal of Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4000000059604645}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1966572509","https://openalex.org/W1970686025","https://openalex.org/W1972384440","https://openalex.org/W1973043978","https://openalex.org/W1975904015","https://openalex.org/W1984731221","https://openalex.org/W2000212755","https://openalex.org/W2006943139","https://openalex.org/W2017791741","https://openalex.org/W2032014604","https://openalex.org/W2036742807","https://openalex.org/W2037867272","https://openalex.org/W2039661736","https://openalex.org/W2042247954","https://openalex.org/W2042716247","https://openalex.org/W2047410084","https://openalex.org/W2050438059","https://openalex.org/W2059767225","https://openalex.org/W2075458703","https://openalex.org/W2078201958","https://openalex.org/W2083325853","https://openalex.org/W2090353177","https://openalex.org/W2115414533","https://openalex.org/W2119205288","https://openalex.org/W2123187537","https://openalex.org/W2128635872","https://openalex.org/W2157640013","https://openalex.org/W2189072284","https://openalex.org/W4245265700","https://openalex.org/W7009171728"],"related_works":["https://openalex.org/W1973043978","https://openalex.org/W2090353177","https://openalex.org/W2024202349","https://openalex.org/W2017791741","https://openalex.org/W2182582527","https://openalex.org/W1995288143","https://openalex.org/W1984731221","https://openalex.org/W1979384245","https://openalex.org/W2036771706","https://openalex.org/W2141616174"],"abstract_inverted_index":{"Abstract":[0],"Objective":[1],"Extracellular":[2],"sucrase":[3,30,110,125,168],"from":[4],"Termitomyces":[5],"clypeatus":[6],"is":[7],"known":[8],"to":[9,75,93,103],"impart":[10,182],"stability":[11],"and":[12,44,47,64,69,79,136,145,152],"enhance":[13],"activity":[14,57,65,161],"of":[15,20,56,83,98,114,120,124,134,141,162,188],"cellobiase,":[16],"another":[17],"secreted":[18],"glycosidase":[19],"the":[21,77,121,138],"fungus":[22],"through":[23],"co-aggregation":[24],"with":[25,33,112,169],"cellobiase.":[26],"To":[27],"explore":[28],"whether":[29],"can":[31,174,181],"bind":[32],"some":[34],"proteins":[35,46],"like":[36],"Insulin,":[37],"BSA,":[38],"alcohol":[39],"dehydrogenase":[40],"(ADH),":[41],"carbonic":[42,142],"anhydrase":[43],"whey":[45,146],"prevent":[48,176],"their":[49],"dithiothreitol":[50],"(DTT)":[51],"induced/thermal":[52],"aggregation":[53,133,140],"and/or":[54],"loss":[55],"measuring":[58],"by":[59,149],"spectrophotometry,":[60],"gel":[61,88],"filtration":[62,89],"assay":[63],"assays.":[66],"Methods":[67],"MALDI-TOF":[68],"dynamic":[70],"light":[71],"scattering":[72],"were":[73,91],"used":[74,92,102],"assess":[76],"monomeric":[78,122,172],"aggregated":[80],"molecular":[81],"size":[82,173],"sucrase.":[84],"Thermostatted":[85],"spectrophotometric":[86],"assays,":[87],"assays":[90],"study":[94],"protein":[95,177,194],"aggregation.":[96,178],"Fluorescence":[97],"bound":[99],"ANS":[100],"was":[101],"monitor":[104],"temperature":[105],"induced":[106,132],"structural":[107],"changes":[108],"in":[109,193],"together":[111],"determination":[113],"melting":[115],"temperature.":[116],"Results":[117],"The":[118,179],"mass":[119],"unit":[123],"as":[126],"6649":[127],"Da.":[128],"Enzyme":[129],"inhibited":[130],"DTT":[131],"insulin":[135],"suppressed":[137],"thermal":[139],"anhydrase,":[143],"ADH":[144],"proteins,":[147],"respectively":[148],"83%,":[150],"68%":[151],"70%":[153],"at":[154],"70\u00b0C.":[155],"Sucrase":[156],"also":[157],"protected":[158],"about":[159,184],"84%":[160],"ADH.":[163],"Conclusion":[164],"An":[165],"extracellular":[166],"fungal":[167],"a":[170],"low":[171],"efficiently":[175],"studies":[180],"knowledge":[183],"potential":[185],"therapeutic":[186],"applications":[187],"this":[189],"industrially":[190],"important":[191],"enzyme":[192],"misfolding":[195],"disorders.":[196]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-03-16T00:00:00"}
