{"id":"https://openalex.org/W2591567719","doi":"https://doi.org/10.3760/cma.j.issn.1007-9408.2011.06.008","title":"Transient expression and biological activity identification of human pigment epithelium-derived factor in mammary cell line SP2/0","display_name":"Transient expression and biological activity identification of human pigment epithelium-derived factor in mammary cell line SP2/0","publication_year":2011,"publication_date":"2011-06-16","ids":{"openalex":"https://openalex.org/W2591567719","doi":"https://doi.org/10.3760/cma.j.issn.1007-9408.2011.06.008","mag":"2591567719"},"language":"en","primary_location":{"id":"mag:2591567719","doi":"https://doi.org/10.3760/cma.j.issn.1007-9408.2011.06.008","is_oa":false,"landing_page_url":"http://rs.yiigle.com/CN113903201106/160996.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764700186","display_name":"Zhonghua weichan yixue zazhi","issn_l":"1007-9408","issn":["1007-9408"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800525","host_organization_name":"Chinese Medical Association","host_organization_lineage":["https://openalex.org/P4320800525"],"host_organization_lineage_names":["Chinese Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhonghua weichan yixue zazhi","raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000670629","display_name":"Yi Dai","orcid":"https://orcid.org/0000-0002-2696-0179","observed_orcids":["https://orcid.org/0000-0002-2696-0179","https://orcid.org/0009-0000-6845-4541"]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yi Dai","raw_affiliation_strings":["Department of Neonatology, Children\u2032s Hospital of Fudan University, Key Laboratory of Neonatal Disease of Health Ministry, Shanghai 201102, China"],"raw_orcid":"https://orcid.org/0000-0002-2696-0179","affiliations":[{"raw_affiliation_string":"Department of Neonatology, Children\u2032s Hospital of Fudan University, Key Laboratory of Neonatal Disease of Health Ministry, Shanghai 201102, China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059890234","display_name":"Wenjing Shi","orcid":"https://orcid.org/0000-0003-3666-1110","observed_orcids":["https://orcid.org/0000-0003-3666-1110"]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenjing Shi","raw_affiliation_strings":["Department of Neonatology, Children\u2032s Hospital of Fudan University, Key Laboratory of Neonatal Disease of Health Ministry, Shanghai 201102, China"],"raw_orcid":"https://orcid.org/0000-0003-3666-1110","affiliations":[{"raw_affiliation_string":"Department of Neonatology, Children\u2032s Hospital of Fudan University, Key Laboratory of Neonatal Disease of Health Ministry, Shanghai 201102, China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082493472","display_name":"Yu-xiong Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24943067","display_name":"Fudan University","ror":"https://ror.org/013q1eq08","country_code":"CN","type":"education","lineage":["https://openalex.org/I24943067"]},{"id":"https://openalex.org/I4210159329","display_name":"Children's Hospital of Fudan University","ror":"https://ror.org/05n13be63","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210159329"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yu-xiong Wang","raw_affiliation_strings":["Department of Neonatology, Children\u2032s Hospital of Fudan University, Key Laboratory of Neonatal Disease of Health Ministry, Shanghai 201102, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neonatology, Children\u2032s Hospital of Fudan University, Key Laboratory of Neonatal Disease of Health Ministry, Shanghai 201102, China","institution_ids":["https://openalex.org/I24943067","https://openalex.org/I4210159329"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Min Yu","orcid":"https://orcid.org/0000-0002-3969-8720","observed_orcids":["https://orcid.org/0000-0002-3969-8720"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Min Yu","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-3969-8720","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5100408358","display_name":"Chao Chen","orcid":"https://orcid.org/0000-0002-5114-2282","observed_orcids":["https://orcid.org/0000-0002-5114-2282"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chao Chen","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5114-2282","affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.27089781,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"14","issue":"06","first_page":"347","last_page":"353"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10422","display_name":"Angiogenesis and VEGF in Cancer","score":0.23080000281333923,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10422","display_name":"Angiogenesis and VEGF in Cancer","score":0.23080000281333923,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.2206999957561493,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.08070000261068344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pigment-epithelium-derived-factor","display_name":"pigment epithelium-derived factor","score":0.996999979019165},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8040000200271606},{"id":"https://openalex.org/keywords/transient-expression","display_name":"transient expression","score":0.6119999885559082},{"id":"https://openalex.org/keywords/sp2-0-cells","display_name":"SP2/0 cells","score":0.9549999833106995},{"id":"https://openalex.org/keywords/eukaryotic-expression-vector","display_name":"eukaryotic expression vector","score":0.8510000109672546},{"id":"https://openalex.org/keywords/human-umbilical-vein-endothelial-cells","display_name":"human umbilical vein endothelial cells","score":0.925000011920929},{"id":"https://openalex.org/keywords/endothelial-cell-proliferation-inhibition","display_name":"endothelial cell proliferation inhibition","score":0.9020000100135803},{"id":"https://openalex.org/keywords/retinopathy-of-prematurity","display_name":"retinopathy of prematurity","score":0.9459999799728394},{"id":"https://openalex.org/keywords/serpins","display_name":"serpins","score":0.7360000014305115}],"concepts":[{"id":"https://openalex.org/C52237779","wikidata":"https://www.wikidata.org/wiki/Q18030441","display_name":"PEDF","level":3,"score":0.9657086133956909},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.778830885887146},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7775089740753174},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.706929087638855},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.6366820931434631},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6291300058364868},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6204794645309448},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.5111048817634583},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5086966156959534},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.49133220314979553},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.4265156686306},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3847687840461731},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19515377283096313},{"id":"https://openalex.org/C2780394083","wikidata":"https://www.wikidata.org/wiki/Q539568","display_name":"Angiogenesis","level":2,"score":0.17237421870231628},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0508592426776886}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2591567719","doi":"https://doi.org/10.3760/cma.j.issn.1007-9408.2011.06.008","is_oa":false,"landing_page_url":"http://rs.yiigle.com/CN113903201106/160996.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764700186","display_name":"Zhonghua weichan yixue zazhi","issn_l":"1007-9408","issn":["1007-9408"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4320800525","host_organization_name":"Chinese Medical Association","host_organization_lineage":["https://openalex.org/P4320800525"],"host_organization_lineage_names":["Chinese Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhonghua weichan yixue zazhi","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2096722608","https://openalex.org/W1983561824","https://openalex.org/W102062378","https://openalex.org/W4393166903","https://openalex.org/W2028126321","https://openalex.org/W2061731519","https://openalex.org/W7096138561","https://openalex.org/W1166210684","https://openalex.org/W1531929133","https://openalex.org/W2744097363"],"abstract_inverted_index":{"Objective":[0],"To":[1],"construct":[2],"eukaryotic":[3,43,115,203],"expression":[4,14,44,116,204,227],"plasmid":[5,121,205],"pIRESneo3-pigment":[6],"epithelium-derived":[7],"factor":[8],"(PEDF)":[9],"and":[10,73,97,152,174,211,228,241],"detect":[11],"its":[12],"transient":[13],"in":[15,33,63],"SP2/0":[16,53,64,125],"cells.":[17],"Methods":[18],"Specific":[19],"primers":[20],"were":[21],"designed":[22],"based":[23],"on":[24],"the":[25,34,42,81,102,114,120,143,157,165],"mature":[26,103],"peptide":[27,104],"sequence":[28,105],"of":[29,80,106,145,149,167,183,197,225,230,244,248],"human":[30,59,83,107,184,213],"PEDF":[31,37,48,60,84,108,168,214],"cDNA":[32,109],"GenBank.":[35],"Human":[36],"gene":[38],"was":[39,50,68,85,110,122,137,190,215],"cloned":[40,112],"into":[41,52,113,124],"vector":[45,117],"pIRESneo3.":[46,118],"The":[47,57,77,181,202],"DNA":[49,98],"transfected":[51,123],"with":[54],"LipofectamineTM":[55],"2000.":[56],"recombinant":[58,82],"protein":[61,233],"expressed":[62],"cell":[65,188],"culture":[66],"supernatant":[67,194],"identified":[69],"by":[70,87,193],"Western":[71,131],"blot":[72,132],"enzyme-linked":[74],"immunosorbent":[75,161],"assay.":[76],"biological":[78],"activity":[79],"measured":[86],"3-(4,5-dimethylthiazol-z-y1)-2,5-diphenytetrazolium":[88],"bromide(MTT)":[89],"method.":[90],"Results":[91],"PCR":[92],"amplification,":[93],"restriction":[94],"enzyme":[95],"digestion":[96],"sequencing":[99],"confirmed":[100],"that":[101,135,164],"successfully":[111,209],"And":[119],"cells,":[126],"which":[127,218],"could":[128,234],"secret":[129],"PEDF.":[130,231],"analysis":[133],"showed":[134],"there":[136],"only":[138],"one":[139],"obvious":[140],"band":[141],"at":[142,176],"position":[144],"relative":[146],"molecular":[147],"weight":[148],"50":[150],"000,":[151],"it":[153],"is":[154],"equivalent":[155],"to":[156,170],"expected":[158],"value.":[159],"Enzyme-linked":[160],"assay":[162],"suggested":[163],"content":[166],"began":[169],"rise":[171],"after":[172,195],"transfection,":[173],"peaked":[175],"36":[177,198],"h":[178,199],"[(0.92\u00b10.04)":[179],"\u03bcg/ml].":[180],"proliferation":[182],"umbilical":[185],"vein":[186],"endothelial":[187],"line":[189],"significantly":[191],"inhibited":[192],"transfection":[196],"(P<0.05).":[200],"Conclusions":[201],"pIRESneo3-PEDF":[206],"had":[207],"been":[208],"constructed":[210],"active":[212],"transiently":[216],"secreted,":[217],"made":[219],"a":[220,236],"foundation":[221],"for":[222,239],"further":[223],"study":[224],"stable":[226],"purification":[229],"This":[232],"be":[235],"potential":[237],"medication":[238],"preventing":[240],"managing":[242],"retinopathy":[243],"prematurity.\r\n\r\nKey":[245],"words:":[246],"\r\nRetinopathy":[247],"prematurity;\u00a0":[249],"Eye":[250],"proteins;\u00a0":[251],"Nerve":[252],"growth":[253],"factors;\u00a0":[254],"Serpins;\u00a0":[255],"Eukaryotic":[256],"cells":[257]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2017-03-03T00:00:00"}
