{"id":"https://openalex.org/W2590764755","doi":"https://doi.org/10.1200/jco.2005.23.16_suppl.9541","title":"Reactivation of the tumor suppressor RASSF1A by selective depletion of DNA methyltransferase 1 (DNMT1) by MG98 sensitizes renal cancer cells to interferon (IFN)-induced apoptosis","display_name":"Reactivation of the tumor suppressor RASSF1A by selective depletion of DNA methyltransferase 1 (DNMT1) by MG98 sensitizes renal cancer cells to interferon (IFN)-induced apoptosis","publication_year":2005,"publication_date":"2005-06-01","ids":{"openalex":"https://openalex.org/W2590764755","doi":"https://doi.org/10.1200/jco.2005.23.16_suppl.9541","mag":"2590764755"},"language":"en","primary_location":{"id":"doi:10.1200/jco.2005.23.16_suppl.9541","doi":"https://doi.org/10.1200/jco.2005.23.16_suppl.9541","is_oa":false,"landing_page_url":"https://doi.org/10.1200/jco.2005.23.16_suppl.9541","pdf_url":null,"source":{"id":"https://openalex.org/S15137598","display_name":"Journal of Clinical Oncology","issn_l":"0732-183X","issn":["0732-183X","1527-7755"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Oncology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071804307","display_name":"Frederic J. Reu","orcid":"https://orcid.org/0000-0003-1160-8030","observed_orcids":["https://orcid.org/0000-0003-1160-8030"]},"institutions":[{"id":"https://openalex.org/I1316902750","display_name":"Cleveland Clinic","ror":"https://ror.org/03xjacd83","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316902750"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F. J. Reu","raw_affiliation_strings":["The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada","institution_ids":["https://openalex.org/I1316902750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108514263","display_name":"Ratan K. Maitra","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1316902750","display_name":"Cleveland Clinic","ror":"https://ror.org/03xjacd83","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316902750"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. K. Maitra","raw_affiliation_strings":["The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada","institution_ids":["https://openalex.org/I1316902750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077654106","display_name":"Donald Rempinski","orcid":"https://orcid.org/0009-0002-3147-7680","observed_orcids":["https://orcid.org/0009-0002-3147-7680"]},"institutions":[{"id":"https://openalex.org/I1316902750","display_name":"Cleveland Clinic","ror":"https://ror.org/03xjacd83","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316902750"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. R. Rempinski","raw_affiliation_strings":["The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada","institution_ids":["https://openalex.org/I1316902750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100858354","display_name":"Songa Bae","orcid":"https://orcid.org/0000-0003-0411-5692","observed_orcids":["https://orcid.org/0000-0003-0411-5692"]},"institutions":[{"id":"https://openalex.org/I1316902750","display_name":"Cleveland Clinic","ror":"https://ror.org/03xjacd83","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316902750"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S. I. Bae","raw_affiliation_strings":["The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada","institution_ids":["https://openalex.org/I1316902750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009373788","display_name":"Normand Beaulieu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1316902750","display_name":"Cleveland Clinic","ror":"https://ror.org/03xjacd83","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316902750"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N. Beaulieu","raw_affiliation_strings":["The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada","institution_ids":["https://openalex.org/I1316902750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103250386","display_name":"A. Robert MacLeod","orcid":"https://orcid.org/0000-0002-8092-1840","observed_orcids":["https://orcid.org/0000-0002-8092-1840"]},"institutions":[{"id":"https://openalex.org/I1316902750","display_name":"Cleveland Clinic","ror":"https://ror.org/03xjacd83","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316902750"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A. R. Macleod","raw_affiliation_strings":["The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada","institution_ids":["https://openalex.org/I1316902750"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112252438","display_name":"Ernest C. Borden","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1316902750","display_name":"Cleveland Clinic","ror":"https://ror.org/03xjacd83","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316902750"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"E. C. Borden","raw_affiliation_strings":["The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Cleveland Clinic Fdn, Cleveland, OH; MethylGene, Inc, Montreal, PQ, Canada","institution_ids":["https://openalex.org/I1316902750"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1316902750"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"23","issue":"16_suppl","first_page":"9541","last_page":"9541"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10449","display_name":"Renal cell carcinoma treatment","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10515","display_name":"Cancer-related molecular mechanisms research","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dnmt1","display_name":"DNMT1","score":0.75},{"id":"https://openalex.org/keywords/renal-cell-carcinoma","display_name":"renal cell carcinoma","score":0.7799999713897705},{"id":"https://openalex.org/keywords/dna-demethylation","display_name":"DNA demethylation","score":0.8830000162124634},{"id":"https://openalex.org/keywords/epigenetic-silencing","display_name":"epigenetic silencing","score":0.7360000014305115},{"id":"https://openalex.org/keywords/tumor-suppressor-gene-reactivation","display_name":"tumor suppressor gene reactivation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/interferon-resistance","display_name":"interferon resistance","score":0.7279999852180481},{"id":"https://openalex.org/keywords/achn-cells","display_name":"ACHN cells","score":0.8140000104904175},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.8560000061988831},{"id":"https://openalex.org/keywords/lentiviral-overexpression","display_name":"lentiviral overexpression","score":0.5910000205039978},{"id":"https://openalex.org/keywords/dna-methylation","display_name":"DNA methylation","score":0.46000000834465027},{"id":"https://openalex.org/keywords/interferon-beta","display_name":"interferon-beta","score":0.35899999737739563}],"concepts":[{"id":"https://openalex.org/C175211621","wikidata":"https://www.wikidata.org/wiki/Q14907998","display_name":"DNMT1","level":5,"score":0.7961670160293579},{"id":"https://openalex.org/C196795494","wikidata":"https://www.wikidata.org/wiki/Q1624817","display_name":"TUNEL assay","level":3,"score":0.7528576254844666},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.7055679559707642},{"id":"https://openalex.org/C190727270","wikidata":"https://www.wikidata.org/wiki/Q874745","display_name":"DNA methylation","level":4,"score":0.6464569568634033},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.6050697565078735},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5954084396362305},{"id":"https://openalex.org/C16824249","wikidata":"https://www.wikidata.org/wiki/Q5205753","display_name":"DNA demethylation","level":5,"score":0.5788713693618774},{"id":"https://openalex.org/C2779979206","wikidata":"https://www.wikidata.org/wiki/Q1154602","display_name":"DNA methyltransferase","level":5,"score":0.5280683040618896},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5275185704231262},{"id":"https://openalex.org/C91965660","wikidata":"https://www.wikidata.org/wiki/Q415875","display_name":"Methyltransferase","level":4,"score":0.5258479714393616},{"id":"https://openalex.org/C2779277951","wikidata":"https://www.wikidata.org/wiki/Q5255196","display_name":"Demethylating agent","level":5,"score":0.4816102683544159},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.47222140431404114},{"id":"https://openalex.org/C2778264664","wikidata":"https://www.wikidata.org/wiki/Q422445","display_name":"Tumor suppressor gene","level":4,"score":0.4478250741958618},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.4178348481655121},{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.3921213150024414},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.28783291578292847},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.20065999031066895},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.1979336142539978},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.17001774907112122},{"id":"https://openalex.org/C555283112","wikidata":"https://www.wikidata.org/wiki/Q1637543","display_name":"Carcinogenesis","level":3,"score":0.12877163290977478},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09434756636619568},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0642993152141571},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1200/jco.2005.23.16_suppl.9541","doi":"https://doi.org/10.1200/jco.2005.23.16_suppl.9541","is_oa":false,"landing_page_url":"https://doi.org/10.1200/jco.2005.23.16_suppl.9541","pdf_url":null,"source":{"id":"https://openalex.org/S15137598","display_name":"Journal of Clinical Oncology","issn_l":"0732-183X","issn":["0732-183X","1527-7755"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Oncology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2096217287","https://openalex.org/W2017130328","https://openalex.org/W1663536395","https://openalex.org/W2132622781","https://openalex.org/W3180064455","https://openalex.org/W2084733717","https://openalex.org/W3172860199","https://openalex.org/W1761825801","https://openalex.org/W2046893938","https://openalex.org/W4231111178"],"abstract_inverted_index":{"9541":[0],"Background:":[1],"Epigenetic":[2],"mechanisms":[3],"may":[4,266],"underlie":[5],"resistance":[6,224],"of":[7,44,93,104,216,225,270,280,291],"renal":[8,29,49],"cell":[9],"carcinoma":[10],"(RCC)":[11],"to":[12,66,80,115,127,152,184,210,228],"therapies":[13],"including":[14],"IFNs.":[15,67],"RASSF1A":[16,94,188,193,196,217],"is":[17],"the":[18,55,285],"tumor":[19],"suppressor":[20],"gene":[21],"most":[22],"frequently":[23],"silenced":[24],"by":[25,87,97,111,118,130,220,263,278],"DNA":[26,45,83,119,221,260],"methylation":[27,46,88],"in":[28,32,175,254,272,276,295],"cancer.":[30],"Functions":[31],"mitosis":[33],"control":[34,208],"and":[35,72,99,162,191,239,293],"apoptosis":[36,128,153,176,204,230,255],"have":[37],"been":[38],"described.":[39],"Methods:":[40],"To":[41],"inhibit":[42],"maintenance":[43],"ACHN":[47,123,226],"human":[48],"cancer":[50],"cells":[51,124,227],"were":[52,78,125],"treated":[53],"with":[54,102,154,207],"DNMT1":[56,182],"selective":[57],"phosphorothioate":[58],"antisense":[59],"inhibitor":[60],"MG98":[61,141,167,264,294],"(DNMT1":[62,142,148,168],"AS)":[63,169],"before":[64],"exposure":[65],"Flow":[68],"cytometric":[69],"TUNEL":[70,139,164,178,241,257],"analysis":[71],"immunoblotting":[73],"for":[74],"caspase":[75],"3":[76],"cleavage":[77],"used":[79],"measure":[81],"apoptosis.":[82],"demethylation":[84,222,261],"was":[85,95],"assessed":[86],"specific":[89],"PCR":[90],"(MSP).":[91],"Role":[92,305],"examined":[96],"siRNA":[98,197,209],"lentiviral":[100,249],"expression":[101,215],"selection":[103],"clones":[105,250],"expressing":[106],"physiologic":[107],"levels,":[108,186],"as":[109],"determined":[110],"comparison":[112],"on":[113],"immunoblots":[114],"levels":[116,218],"achieved":[117],"demethylation.":[120],"Results:":[121],"Although":[122],"resistant":[126],"induction":[129],"IFN-a2":[131,158,292],"or":[132,159,300,303],"-\u03b2":[133,160],"even":[134],"at":[135,274],"500":[136],"U/ml":[137,157,246],"(<5%":[138,177,256],"positive),":[140],"AS),":[143],"but":[144,180],"not":[145,173,235],"mismatch":[146],"oligonucleotide":[147],"MM)":[149],"pretreatment":[150,170],"led":[151],"only":[155],"50":[156,245],"(25\u201350%":[161],"50\u201380%":[163],"positive,":[165,242],"respectively).":[166],"alone":[171],"did":[172,234],"result":[174],"positive)":[179],"decreased":[181],"protein":[183,194],"undetectable":[185],"demethylated":[187],"promoter":[189],"region,":[190],"reactivated":[192],"expression.":[195],"transfection":[198],"following":[199],"demethylating":[200],"treatment":[201,265],"reduced":[202],"IFN-induced":[203,229],"from":[205],"57%":[206],"32%":[211],"(TUNEL).":[212],"Lentiviral":[213],"stable":[214],"achievable":[219],"overcame":[223],"while":[231],"empty":[232],"virus":[233],"sensitize":[236],"(about":[237],"60%":[238],"5%":[240],"respectively,":[243],"after":[244],"IFN-\u03b2).":[247],"Untreated":[248],"had":[251],"no":[252],"increase":[253],"positive).":[258],"Conclusions:":[259],"induced":[262],"enhance":[267],"antitumor":[268],"effect":[269],"IFNs":[271],"RCC":[273],"least":[275],"part":[277],"reactivation":[279],"RASSF1A.":[281],"These":[282],"results":[283],"support":[284],"ongoing":[286],"phase":[287],"II":[288],"clinical":[289],"trial":[290],"RCC.":[296],"Author":[297],"Disclosure":[298],"Employment":[299],"Leadership":[301],"Consultant":[302],"Advisory":[304],"Stock":[306],"Ownership":[307],"Honoraria":[308],"Research":[309],"Funding":[310],"Expert":[311],"Testimony":[312],"Other":[313],"Remuneration":[314],"MethylGene":[315,316]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-07-14T23:27:15.235271","created_date":"2017-03-03T00:00:00"}