{"id":"https://openalex.org/W2586525814","doi":"https://doi.org/10.1038/ncomms14207","title":"Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo","display_name":"Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo","publication_year":2017,"publication_date":"2017-02-06","ids":{"openalex":"https://openalex.org/W2586525814","doi":"https://doi.org/10.1038/ncomms14207","mag":"2586525814","pmid":"https://pubmed.ncbi.nlm.nih.gov/28165449","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5303826"},"language":"en","primary_location":{"id":"doi:10.1038/ncomms14207","doi":"https://doi.org/10.1038/ncomms14207","is_oa":true,"landing_page_url":"https://doi.org/10.1038/ncomms14207","pdf_url":"https://www.nature.com/articles/ncomms14207.pdf","source":{"id":"https://openalex.org/S64187185","display_name":"Nature Communications","issn_l":"2041-1723","issn":["2041-1723"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Communications","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/ncomms14207.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016040987","display_name":"Taro Ozaki","orcid":"https://orcid.org/0000-0002-4673-4478","observed_orcids":["https://orcid.org/0000-0002-4673-4478"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Taro Ozaki","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051074568","display_name":"Kona Yamashita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kona Yamashita","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058688721","display_name":"Yuki Goto","orcid":"https://orcid.org/0000-0003-4317-0790","observed_orcids":["https://orcid.org/0000-0003-4317-0790"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuki Goto","raw_affiliation_strings":["Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan","Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, 113-0033, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, 113-0033, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066561555","display_name":"Morito Shimomura","orcid":"https://orcid.org/0000-0002-9330-3726","observed_orcids":["https://orcid.org/0000-0002-9330-3726"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Morito Shimomura","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Shohei Hayashi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shohei Hayashi","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016856050","display_name":"Shumpei Asamizu","orcid":"https://orcid.org/0000-0002-7178-5410","observed_orcids":["https://orcid.org/0000-0002-7178-5410"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shumpei Asamizu","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081539078","display_name":"Yoshinori Sugai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshinori Sugai","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, 113-8657, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051175802","display_name":"Haruo Ikeda","orcid":"https://orcid.org/0000-0003-3977-7856","observed_orcids":["https://orcid.org/0000-0003-3977-7856"]},"institutions":[{"id":"https://openalex.org/I64189623","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25","country_code":"JP","type":"education","lineage":["https://openalex.org/I64189623"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Haruo Ikeda","raw_affiliation_strings":["Kitasato Institute for Life Sciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan","Kitasato Institute for Life Sciences, Kitasato University, Sagamihara, 252-0373, Kanagawa, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kitasato Institute for Life Sciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan","institution_ids":["https://openalex.org/I64189623"]},{"raw_affiliation_string":"Kitasato Institute for Life Sciences, Kitasato University, Sagamihara, 252-0373, Kanagawa, Japan","institution_ids":["https://openalex.org/I64189623"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017224243","display_name":"Hiroaki Suga","orcid":"https://orcid.org/0000-0002-5298-9186","observed_orcids":["https://orcid.org/0000-0002-5298-9186"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hiroaki Suga","raw_affiliation_strings":["Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan","Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, 113-0033, Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, 113-0033, Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085100374","display_name":"Hiroyasu Onaka","orcid":"https://orcid.org/0000-0003-4536-1881","observed_orcids":["https://orcid.org/0000-0003-4536-1881"]},"institutions":[{"id":"https://openalex.org/I42766147","display_name":"University of Toyama","ror":"https://ror.org/0445phv87","country_code":"JP","type":"education","lineage":["https://openalex.org/I42766147"]},{"id":"https://openalex.org/I63216439","display_name":"Toyama Prefectural University","ror":"https://ror.org/03xgh2v50","country_code":"JP","type":"education","lineage":["https://openalex.org/I63216439"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hiroyasu Onaka","raw_affiliation_strings":["Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","Biotechnology Research Center, Toyama Prefectural University, Imizu, 939-0398, Toyama, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan","institution_ids":["https://openalex.org/I42766147","https://openalex.org/I63216439"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Biotechnology Research Center, Toyama Prefectural University, Imizu, 939-0398, Toyama, Japan","institution_ids":["https://openalex.org/I42766147","https://openalex.org/I63216439"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5017224243","https://openalex.org/A5085100374"],"corresponding_institution_ids":["https://openalex.org/I42766147","https://openalex.org/I63216439","https://openalex.org/I74801974"],"apc_list":{"value":6990,"currency":"USD","value_usd":6990},"apc_paid":{"value":6990,"currency":"USD","value_usd":6990},"fwci":5.023,"has_fulltext":true,"cited_by_count":84,"citation_normalized_percentile":{"value":0.96954406,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":99},"biblio":{"volume":"8","issue":"1","first_page":"14207","last_page":"14207"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.007600000128149986,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ribosomally-synthesized-and-post-translationally-modified-peptides","display_name":"ribosomally synthesized and post-translationally modified peptides","score":0.972000002861023},{"id":"https://openalex.org/keywords/ripp-biosynthesis","display_name":"RiPP biosynthesis","score":0.8730000257492065},{"id":"https://openalex.org/keywords/in-vitro-reconstitution","display_name":"in vitro reconstitution","score":0.8209999799728394},{"id":"https://openalex.org/keywords/dehydroalanine","display_name":"dehydroalanine","score":0.6330000162124634},{"id":"https://openalex.org/keywords/in-vivo-expression","display_name":"in vivo expression","score":0.5239999890327454}],"concepts":[{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.77027428150177},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.7067981362342834},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.603104829788208},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5896205902099609},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5627146363258362},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5605810880661011},{"id":"https://openalex.org/C2776568683","wikidata":"https://www.wikidata.org/wiki/Q23005640","display_name":"Moiety","level":2,"score":0.5410081744194031},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.4588722288608551},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.4313432574272156},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.4303722381591797},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4295620918273926},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4272307753562927},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.36289048194885254},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3311947286128998},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3277830481529236},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.26057112216949463},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.1990174651145935},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1509043276309967}],"mesh":[{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011499","descriptor_name":"Protein Processing, Post-Translational","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015195","descriptor_name":"Drug Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036341","descriptor_name":"Intercellular Signaling Peptides and Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":7,"locations":[{"id":"doi:10.1038/ncomms14207","doi":"https://doi.org/10.1038/ncomms14207","is_oa":true,"landing_page_url":"https://doi.org/10.1038/ncomms14207","pdf_url":"https://www.nature.com/articles/ncomms14207.pdf","source":{"id":"https://openalex.org/S64187185","display_name":"Nature Communications","issn_l":"2041-1723","issn":["2041-1723"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Communications","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:4178994","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5303826","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5303826","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5303826","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nat Commun","raw_type":"Text"},{"id":"pmid:28165449","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28165449","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature communications","raw_type":"Journal Article"},{"id":"pmh:oai:RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14207","doi":null,"is_oa":false,"landing_page_url":"https://www.nature.com/articles/ncomms14207","pdf_url":null,"source":{"id":"https://openalex.org/S4306401271","display_name":"RePEc: Research Papers in Economics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I77793887","host_organization_name":"Federal Reserve Bank of St. Louis","host_organization_lineage":["https://openalex.org/I77793887"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article"},{"id":"pmh:oai:doaj.org/article:b21b652ae3eb468b9440c69d70205317","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/b21b652ae3eb468b9440c69d70205317","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)","raw_type":"article"},{"id":"pmh:oai:irdb.nii.ac.jp:00994:0006612576","doi":null,"is_oa":false,"landing_page_url":"https://ir.lib.shimane-u.ac.jp/53384","pdf_url":null,"source":{"id":"https://openalex.org/S7407056385","display_name":"Institutional Repositories DataBase (IRDB)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I184597095","host_organization_name":"National Institute of Informatics","host_organization_lineage":["https://openalex.org/I184597095"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"NATURE COMMUNICATIONS","raw_type":"journal article"}],"best_oa_location":{"id":"doi:10.1038/ncomms14207","doi":"https://doi.org/10.1038/ncomms14207","is_oa":true,"landing_page_url":"https://doi.org/10.1038/ncomms14207","pdf_url":"https://www.nature.com/articles/ncomms14207.pdf","source":{"id":"https://openalex.org/S64187185","display_name":"Nature Communications","issn_l":"2041-1723","issn":["2041-1723"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Communications","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3216527379","display_name":"Development of artificial natural products","funder_award_id":"15K12739","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G4460085203","display_name":"Analyses of secondary metabolism gene promoters activated by combined-culture","funder_award_id":"26850044","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8393061762","display_name":null,"funder_award_id":"16H06444","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8497079110","display_name":null,"funder_award_id":"JP16H06444","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G999459909","display_name":"Studies on the mechanism of action of goadsporin and generation of new derivatives","funder_award_id":"26850043","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320310088","display_name":"Institute for Fermentation, Osaka","ror":"https://ror.org/05nq89q24"},{"id":"https://openalex.org/F4320322832","display_name":"University of Tokyo","ror":"https://ror.org/057zh3y96"},{"id":"https://openalex.org/F4320324670","display_name":"Kitasato University","ror":"https://ror.org/00f2txz25"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"},{"id":"https://openalex.org/F4320338075","display_name":"Core Research for Evolutional Science and Technology","ror":"https://ror.org/00097mb19"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2586525814.pdf","grobid_xml":"https://content.openalex.org/works/W2586525814.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W1587481053","https://openalex.org/W1587997563","https://openalex.org/W1950387943","https://openalex.org/W1955057702","https://openalex.org/W1985857378","https://openalex.org/W1993689968","https://openalex.org/W2015381774","https://openalex.org/W2017607185","https://openalex.org/W2036874847","https://openalex.org/W2046976964","https://openalex.org/W2048989067","https://openalex.org/W2052195617","https://openalex.org/W2054136420","https://openalex.org/W2059052375","https://openalex.org/W2068057782","https://openalex.org/W2070424221","https://openalex.org/W2077471730","https://openalex.org/W2079343867","https://openalex.org/W2081312943","https://openalex.org/W2081949671","https://openalex.org/W2084552689","https://openalex.org/W2087181770","https://openalex.org/W2087257419","https://openalex.org/W2106246537","https://openalex.org/W2108107434","https://openalex.org/W2141737063","https://openalex.org/W2162481919","https://openalex.org/W2162854463","https://openalex.org/W2163029110","https://openalex.org/W2186689554","https://openalex.org/W2231071237","https://openalex.org/W2280897757","https://openalex.org/W2401324946","https://openalex.org/W2412142794","https://openalex.org/W2416680778","https://openalex.org/W2467914015","https://openalex.org/W2472623803"],"related_works":["https://openalex.org/W2979521193","https://openalex.org/W4406150930","https://openalex.org/W7027777282","https://openalex.org/W7134037671","https://openalex.org/W3087348949","https://openalex.org/W2810643020","https://openalex.org/W4229056741","https://openalex.org/W1997571224","https://openalex.org/W7128593918","https://openalex.org/W4391574562"],"abstract_inverted_index":{"Goadsporin":[0],"(GS)":[1],"is":[2,131],"a":[3,54],"member":[4],"of":[5,40,57,104,145],"ribosomally":[6],"synthesized":[7],"and":[8,20],"post-translationally":[9],"modified":[10],"peptides":[11],"(RiPPs),":[12],"containing":[13],"an":[14],"N-terminal":[15],"acetyl":[16],"moiety,":[17],"six":[18],"azoles":[19],"two":[21],"dehydroalanines":[22],"in":[23,32,63,66,110,123,128],"the":[24,29,38,42,46,60,64,73,85,101,143],"peptidic":[25],"main":[26],"chain.":[27],"Although":[28],"enzymes":[30,44,61],"involved":[31],"GS":[33,58,121],"biosynthesis":[34],"have":[35],"been":[36],"defined,":[37],"principle":[39,144],"how":[41],"respective":[43],"control":[45],"specific":[47],"modifications":[48],"remains":[49],"elusive.":[50],"Here":[51],"we":[52],"report":[53],"one-pot":[55],"synthesis":[56],"using":[59],"reconstituted":[62],"'flexible'":[65],"vitro":[67,111],"translation":[68],"system,":[69],"referred":[70],"to":[71,80,99,117,134,140],"as":[72],"FIT-GS":[74],"system.":[75],"This":[76],"system":[77],"allows":[78],"us":[79,98,116,139],"readily":[81],"prepare":[82],"not":[83],"only":[84],"precursor":[86],"peptide":[87],"from":[88],"its":[89],"synthetic":[90],"DNA":[91],"template":[92],"but":[93],"also":[94,114,132],"52":[95],"mutants,":[96],"enabling":[97],"dissect":[100],"modification":[102,146],"determinants":[103],"GodA":[105],"for":[106],"each":[107],"enzyme.":[108],"The":[109,125],"knowledge":[112],"has":[113],"led":[115],"successfully":[118],"produce":[119],"designer":[120],"analogues":[122],"vivo.":[124],"methodology":[126],"demonstrated":[127],"this":[129],"work":[130],"applicable":[133],"other":[135],"RiPP":[136],"biosynthesis,":[137],"allowing":[138],"rapidly":[141],"investigate":[142],"events":[147],"with":[148],"great":[149],"ease.":[150]},"counts_by_year":[{"year":2026,"cited_by_count":8},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":16},{"year":2020,"cited_by_count":15},{"year":2019,"cited_by_count":8},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-02-17T00:00:00"}