{"id":"https://openalex.org/W2586410656","doi":"https://doi.org/10.1186/s13071-017-2003-1","title":"Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein","display_name":"Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein","publication_year":2017,"publication_date":"2017-02-07","ids":{"openalex":"https://openalex.org/W2586410656","doi":"https://doi.org/10.1186/s13071-017-2003-1","mag":"2586410656","pmid":"https://pubmed.ncbi.nlm.nih.gov/28173829","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5297187"},"language":"en","primary_location":{"id":"doi:10.1186/s13071-017-2003-1","doi":"https://doi.org/10.1186/s13071-017-2003-1","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13071-017-2003-1","pdf_url":"https://parasitesandvectors.biomedcentral.com/counter/pdf/10.1186/s13071-017-2003-1","source":{"id":"https://openalex.org/S80413395","display_name":"Parasites & Vectors","issn_l":"1756-3305","issn":["1756-3305"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasites & Vectors","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://parasitesandvectors.biomedcentral.com/counter/pdf/10.1186/s13071-017-2003-1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063461715","display_name":"Aruna Shrestha","orcid":"https://orcid.org/0000-0002-4929-5861","observed_orcids":["https://orcid.org/0000-0002-4929-5861"]},"institutions":[{"id":"https://openalex.org/I150540706","display_name":"University of Veterinary Medicine Vienna","ror":"https://ror.org/01w6qp003","country_code":"AT","type":"education","lineage":["https://openalex.org/I150540706"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Aruna Shrestha","raw_affiliation_strings":["Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria","institution_ids":["https://openalex.org/I150540706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024137648","display_name":"Nicola Palmieri","orcid":"https://orcid.org/0000-0002-6164-1843","observed_orcids":["https://orcid.org/0000-0002-6164-1843"]},"institutions":[{"id":"https://openalex.org/I150540706","display_name":"University of Veterinary Medicine Vienna","ror":"https://ror.org/01w6qp003","country_code":"AT","type":"education","lineage":["https://openalex.org/I150540706"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Nicola Palmieri","raw_affiliation_strings":["Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria","institution_ids":["https://openalex.org/I150540706"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ahmed Abd-Elfattah","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150540706","display_name":"University of Veterinary Medicine Vienna","ror":"https://ror.org/01w6qp003","country_code":"AT","type":"education","lineage":["https://openalex.org/I150540706"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Ahmed Abd-Elfattah","raw_affiliation_strings":["Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria","institution_ids":["https://openalex.org/I150540706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021579176","display_name":"B\u00e4rbel Ruttkowski","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150540706","display_name":"University of Veterinary Medicine Vienna","ror":"https://ror.org/01w6qp003","country_code":"AT","type":"education","lineage":["https://openalex.org/I150540706"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"B\u00e4rbel Ruttkowski","raw_affiliation_strings":["Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria","institution_ids":["https://openalex.org/I150540706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073881381","display_name":"Marc Pag\u00e8s","orcid":"https://orcid.org/0009-0005-1073-4248","observed_orcids":["https://orcid.org/0009-0005-1073-4248"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Marc Pag\u00e8s","raw_affiliation_strings":["HIPRA, Amer, 17170, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"HIPRA, Amer, 17170, Spain","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088570452","display_name":"Anja Joachim","orcid":"https://orcid.org/0000-0003-3082-6885","observed_orcids":["https://orcid.org/0000-0003-3082-6885"]},"institutions":[{"id":"https://openalex.org/I150540706","display_name":"University of Veterinary Medicine Vienna","ror":"https://ror.org/01w6qp003","country_code":"AT","type":"education","lineage":["https://openalex.org/I150540706"]}],"countries":["AT"],"is_corresponding":true,"raw_author_name":"Anja Joachim","raw_affiliation_strings":["Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria. Anja.Joachim@vetmeduni.ac.at","Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria. Anja.Joachim@vetmeduni.ac.at","institution_ids":["https://openalex.org/I150540706"]},{"raw_affiliation_string":"Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, Vienna, A-1210, Austria","institution_ids":["https://openalex.org/I150540706"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5088570452"],"corresponding_institution_ids":["https://openalex.org/I150540706"],"apc_list":{"value":3090,"currency":"USD","value_usd":3090},"apc_paid":{"value":3090,"currency":"USD","value_usd":3090},"fwci":1.7447,"has_fulltext":true,"cited_by_count":8,"citation_normalized_percentile":{"value":0.81280882,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"10","issue":"1","first_page":"68","last_page":"68"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12691","display_name":"Coccidia and coccidiosis research","score":0.9765999913215637,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12691","display_name":"Coccidia and coccidiosis research","score":0.9765999913215637,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11155","display_name":"Toxoplasma gondii Research Studies","score":0.012400000356137753,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11086","display_name":"Parasitic Infections and Diagnostics","score":0.00930000003427267,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cystoisospora-suis","display_name":"Cystoisospora suis","score":1.0},{"id":"https://openalex.org/keywords/histidine-rich-proteins","display_name":"histidine-rich proteins","score":0.7960000038146973},{"id":"https://openalex.org/keywords/vaccine-candidate","display_name":"vaccine candidate","score":0.7649999856948853},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8989999890327454},{"id":"https://openalex.org/keywords/antigenicity","display_name":"antigenicity","score":0.5080000162124634},{"id":"https://openalex.org/keywords/stage-specific-gene-expression","display_name":"stage-specific gene expression","score":0.6779999732971191},{"id":"https://openalex.org/keywords/subcellular-localization","display_name":"subcellular localization","score":0.7990000247955322},{"id":"https://openalex.org/keywords/parasite-surface-proteins","display_name":"parasite surface proteins","score":0.4399999976158142},{"id":"https://openalex.org/keywords/apicomplexa","display_name":"Apicomplexa","score":0.5400000214576721},{"id":"https://openalex.org/keywords/piglet-infection","display_name":"piglet infection","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9065022468566895},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7107841968536377},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.6256768703460693},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5685884356498718},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.5488128066062927},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5228664875030518},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.45259571075439453},{"id":"https://openalex.org/C190612196","wikidata":"https://www.wikidata.org/wiki/Q180502","display_name":"Parasitology","level":2,"score":0.4271129369735718},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3342447280883789},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18290776014328003},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016053","descriptor_name":"RNA, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016054","descriptor_name":"DNA, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D021743","descriptor_name":"Eimeriidae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D021743","descriptor_name":"Eimeriidae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D034101","descriptor_name":"Sporozoites","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D052940","descriptor_name":"Merozoites","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1186/s13071-017-2003-1","doi":"https://doi.org/10.1186/s13071-017-2003-1","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13071-017-2003-1","pdf_url":"https://parasitesandvectors.biomedcentral.com/counter/pdf/10.1186/s13071-017-2003-1","source":{"id":"https://openalex.org/S80413395","display_name":"Parasites & Vectors","issn_l":"1756-3305","issn":["1756-3305"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasites & Vectors","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:4167804","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5297187","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5297187","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5297187","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasit Vectors","raw_type":"Text"},{"id":"pmid:28173829","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28173829","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasites & vectors","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1186/s13071-017-2003-1","doi":"https://doi.org/10.1186/s13071-017-2003-1","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13071-017-2003-1","pdf_url":"https://parasitesandvectors.biomedcentral.com/counter/pdf/10.1186/s13071-017-2003-1","source":{"id":"https://openalex.org/S80413395","display_name":"Parasites & Vectors","issn_l":"1756-3305","issn":["1756-3305"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Parasites & Vectors","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.7246000170707703,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320325611","display_name":"Veterin\u00e4rmedizinische Universit\u00e4t Wien","ror":"https://ror.org/01w6qp003"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2586410656.pdf","grobid_xml":"https://content.openalex.org/works/W2586410656.grobid-xml"},"referenced_works_count":66,"referenced_works":["https://openalex.org/W1756115834","https://openalex.org/W1884908391","https://openalex.org/W1953335322","https://openalex.org/W1966513342","https://openalex.org/W1971731019","https://openalex.org/W1971756877","https://openalex.org/W1972084678","https://openalex.org/W1974343083","https://openalex.org/W1974388287","https://openalex.org/W1982659704","https://openalex.org/W1994693805","https://openalex.org/W1996490750","https://openalex.org/W1999389035","https://openalex.org/W2001158263","https://openalex.org/W2003647795","https://openalex.org/W2010017315","https://openalex.org/W2011944521","https://openalex.org/W2012034410","https://openalex.org/W2012226499","https://openalex.org/W2016823905","https://openalex.org/W2017030663","https://openalex.org/W2025780168","https://openalex.org/W2034921395","https://openalex.org/W2040165635","https://openalex.org/W2041008969","https://openalex.org/W2042278106","https://openalex.org/W2045525756","https://openalex.org/W2046371898","https://openalex.org/W2049601398","https://openalex.org/W2049809596","https://openalex.org/W2054294996","https://openalex.org/W2057021995","https://openalex.org/W2060580141","https://openalex.org/W2069295288","https://openalex.org/W2071829542","https://openalex.org/W2073241690","https://openalex.org/W2075870326","https://openalex.org/W2085038375","https://openalex.org/W2089818849","https://openalex.org/W2090079179","https://openalex.org/W2094671950","https://openalex.org/W2101797111","https://openalex.org/W2103047483","https://openalex.org/W2107277218","https://openalex.org/W2110145073","https://openalex.org/W2114360920","https://openalex.org/W2115894398","https://openalex.org/W2118103211","https://openalex.org/W2126120933","https://openalex.org/W2128635872","https://openalex.org/W2130551913","https://openalex.org/W2142044718","https://openalex.org/W2146041009","https://openalex.org/W2146446052","https://openalex.org/W2147364153","https://openalex.org/W2156022009","https://openalex.org/W2160149159","https://openalex.org/W2217900475","https://openalex.org/W2340589786","https://openalex.org/W2343923565","https://openalex.org/W2409097122","https://openalex.org/W2428761793","https://openalex.org/W2583733911","https://openalex.org/W2766801561","https://openalex.org/W4407210889","https://openalex.org/W7075605158"],"related_works":["https://openalex.org/W6921334185","https://openalex.org/W2583733911","https://openalex.org/W2035774979","https://openalex.org/W2380538900","https://openalex.org/W2114317062","https://openalex.org/W2806410115","https://openalex.org/W2357761716","https://openalex.org/W2351553673","https://openalex.org/W2072501365","https://openalex.org/W2369672136"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,67,87,123,163,226],"genome":[2],"of":[3,24,72,89,102,149,188,222],"the":[4,19,22,73,82,90,146,195,209,220,223],"apicomplexan":[5],"parasite":[6],"Cystoisospora":[7],"suis":[8,151,271],"(syn.":[9],"Isospora":[10],"suis)":[11],"has":[12],"recently":[13],"been":[14],"sequenced":[15],"and":[16,60,79,99,108,117,178,202,213,236,244,254],"annotated,":[17],"opening":[18],"possibility":[20],"for":[21,127,241,265],"identification":[23],"novel":[25],"therapeutic":[26],"targets":[27],"against":[28,269],"cystoisosporosis.":[29],"It":[30],"was":[31,76,94,142,166],"previously":[32],"proposed":[33],"that":[34,140,191,229],"a":[35,47,128,134],"42":[36],"kDa":[37],"uncharacterized":[38],"merozoite":[39],"protein,":[40],"encoded":[41,126],"by":[42,113,169],"gene":[43,75,125],"CSUI_005805,":[44],"might":[45,237],"be":[46,239,259],"relevant":[48],"vaccine":[49],"candidate":[50,264],"due":[51],"to":[52,161,250],"its":[53,251],"high":[54,57],"immunogenic":[55],"score,":[56],"expression":[58,84,103,255],"level":[59],"species-specificity":[61],"as":[62,261,273],"determined":[63,112],"in":[64,96,104,152,158,208,234],"silico.":[65],"METHODS:":[66],"1170":[68],"bp":[69],"coding":[70],"sequence":[71],"CSUI_005805":[74,124,141,176,197,230,257],"PCR":[77,116],"amplified":[78],"cloned":[80],"into":[81],"bacterial":[83],"vector":[85],"pQE-31.":[86],"specificity":[88],"expressed":[91,144,233],"recombinant":[92,164,175,196],"protein":[93,132,165,177,198],"evaluated":[95],"an":[97,205,262],"immunoblot,":[98],"relative":[100],"levels":[101,157],"different":[105],"developmental":[106],"stages":[107],"subcellular":[109],"localization":[110,217,253],"were":[111],"quantitative":[114],"real-time":[115],"indirect":[118],"immunofluorescence":[119],"assay,":[120],"respectively.":[121],"RESULTS:":[122],"389":[129],"amino":[130],"acid":[131],"containing":[133],"histidine-rich":[135],"region.":[136],"Quantitative":[137],"RT-PCR":[138],"showed":[139],"differentially":[143],"during":[145],"early":[147],"development":[148],"C.":[150,185,270],"vitro,":[153],"with":[154,174,184],"higher":[155],"transcript":[156],"merozoites":[159,235],"compared":[160],"sporozoites.":[162],"specifically":[167],"recognized":[168],"sera":[170,179],"from":[171,180],"chicken":[172],"immunized":[173],"piglets":[181],"experimentally":[182],"infected":[183],"suis,":[186],"all":[187],"which":[189],"suggested":[190],"despite":[192],"prokaryotic":[193],"expression,":[194],"maintained":[199],"antigenic":[200],"determinants":[201],"could":[203,258],"elicit":[204],"immune":[206],"response":[207],"host.":[210],"Immunofluorescence":[211],"labelling":[212],"confocal":[214],"microscopy":[215],"revealed":[216],"primarily":[218],"at":[219],"surface":[221],"parasite.":[224],"CONCLUSIONS:":[225],"results":[227],"suggest":[228],"is":[231],"highly":[232],"thus":[238],"critical":[240],"their":[242],"survival":[243],"establishment":[245],"inside":[246],"host":[247],"cells.":[248],"Owing":[249],"specificity,":[252],"pattern,":[256],"exploited":[260],"attractive":[263],"alternative":[266],"control":[267],"strategies":[268],"such":[272],"vaccines.":[274]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-02-17T00:00:00"}