{"id":"https://openalex.org/W2585025698","doi":"https://doi.org/10.1007/s13361-016-1591-x","title":"Ambient Ionization Mass Spectrometry Measurement of Aminotransferase Activity","display_name":"Ambient Ionization Mass Spectrometry Measurement of Aminotransferase Activity","publication_year":2017,"publication_date":"2017-01-31","ids":{"openalex":"https://openalex.org/W2585025698","doi":"https://doi.org/10.1007/s13361-016-1591-x","mag":"2585025698","pmid":"https://pubmed.ncbi.nlm.nih.gov/28144898"},"language":"en","primary_location":{"id":"doi:10.1007/s13361-016-1591-x","doi":"https://doi.org/10.1007/s13361-016-1591-x","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s13361-016-1591-x","pdf_url":null,"source":{"id":"https://openalex.org/S60743949","display_name":"Journal of the American Society for Mass Spectrometry","issn_l":"1044-0305","issn":["1044-0305","1879-1123"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051218565","display_name":"Xin Yan","orcid":"https://orcid.org/0000-0002-8292-130X","observed_orcids":["https://orcid.org/0000-0002-8292-130X","https://orcid.org/0009-0006-5668-8870"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]},{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xin Yan","raw_affiliation_strings":["Department of Chemistry, Purdue University, West Lafayette, IN, USA","Department of Chemistry, Stanford University, Stanford, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Purdue University, West Lafayette, IN, USA","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Department of Chemistry, Stanford University, Stanford, CA, USA","institution_ids":["https://openalex.org/I97018004"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Xin Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xin Li","raw_affiliation_strings":["Department of Chemistry, Purdue University, West Lafayette, IN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Purdue University, West Lafayette, IN, USA","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032936797","display_name":"Chengsen Zhang","orcid":"https://orcid.org/0000-0001-5674-0794","observed_orcids":["https://orcid.org/0000-0001-5674-0794"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chengsen Zhang","raw_affiliation_strings":["Department of Chemistry, Purdue University, West Lafayette, IN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Purdue University, West Lafayette, IN, USA","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101575467","display_name":"Yang Xu","orcid":"https://orcid.org/0000-0003-2324-1299","observed_orcids":["https://orcid.org/0000-0003-2324-1299"]},"institutions":[{"id":"https://openalex.org/I194450716","display_name":"Jilin University","ror":"https://ror.org/00js3aw79","country_code":"CN","type":"education","lineage":["https://openalex.org/I194450716"]},{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Yang Xu","raw_affiliation_strings":["College of Life Sciences, Jilin University, Changchun, China","Department of Chemistry, Purdue University, West Lafayette, IN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Life Sciences, Jilin University, Changchun, China","institution_ids":["https://openalex.org/I194450716"]},{"raw_affiliation_string":"Department of Chemistry, Purdue University, West Lafayette, IN, USA","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5036827887","display_name":"R. Graham Cooks","orcid":"https://orcid.org/0000-0002-9581-9603","observed_orcids":["https://orcid.org/0000-0002-9581-9603"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. Graham Cooks","raw_affiliation_strings":["Department of Chemistry, Purdue University, West Lafayette, IN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Purdue University, West Lafayette, IN, USA","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.8406,"has_fulltext":false,"cited_by_count":17,"citation_normalized_percentile":{"value":0.62745727,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":99},"biblio":{"volume":"28","issue":"6","first_page":"1175","last_page":"1181"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aspartate-aminotransferase","display_name":"aspartate aminotransferase","score":0.9120000004768372},{"id":"https://openalex.org/keywords/enzyme-activity-assay","display_name":"enzyme activity assay","score":0.8429999947547913},{"id":"https://openalex.org/keywords/paper-spray-mass-spectrometry","display_name":"paper spray mass spectrometry","score":0.9440000057220459},{"id":"https://openalex.org/keywords/ambient-ionization","display_name":"ambient ionization","score":0.9139999747276306},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5569999814033508},{"id":"https://openalex.org/keywords/clinical-biomarkers","display_name":"clinical biomarkers","score":0.7590000033378601},{"id":"https://openalex.org/keywords/serum-samples","display_name":"serum samples","score":0.6980000138282776},{"id":"https://openalex.org/keywords/desalination","display_name":"desalination","score":0.6880000233650208}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9393836259841919},{"id":"https://openalex.org/C2776923573","wikidata":"https://www.wikidata.org/wiki/Q2294884","display_name":"Derivatization","level":3,"score":0.865440845489502},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7233626842498779},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.7170027494430542},{"id":"https://openalex.org/C2778576202","wikidata":"https://www.wikidata.org/wiki/Q2540164","display_name":"Sample preparation","level":2,"score":0.4423257112503052},{"id":"https://openalex.org/C2778230297","wikidata":"https://www.wikidata.org/wiki/Q1141546","display_name":"Sprayer","level":2,"score":0.42951396107673645},{"id":"https://openalex.org/C187320778","wikidata":"https://www.wikidata.org/wiki/Q1349130","display_name":"Geotechnical engineering","level":1,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000637","descriptor_name":"Transaminases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000637","descriptor_name":"Transaminases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001219","descriptor_name":"Aspartate Aminotransferases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001219","descriptor_name":"Aspartate Aminotransferases","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D001219","descriptor_name":"Aspartate Aminotransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002138","descriptor_name":"Calibration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/s13361-016-1591-x","doi":"https://doi.org/10.1007/s13361-016-1591-x","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s13361-016-1591-x","pdf_url":null,"source":{"id":"https://openalex.org/S60743949","display_name":"Journal of the American Society for Mass Spectrometry","issn_l":"1044-0305","issn":["1044-0305","1879-1123"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"journal-article"},{"id":"pmid:28144898","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28144898","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.46810001134872437}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8544819675","display_name":"Ionic reactions in droplets and thin films for preparative and analytical mass spectrometry","funder_award_id":"1307264","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320337393","display_name":"Division of Chemistry","ror":"https://ror.org/01ar8dr59"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":59,"referenced_works":["https://openalex.org/W153102223","https://openalex.org/W1967246198","https://openalex.org/W1967925645","https://openalex.org/W1975081408","https://openalex.org/W1982991226","https://openalex.org/W1987918216","https://openalex.org/W1995746333","https://openalex.org/W2003495709","https://openalex.org/W2006971413","https://openalex.org/W2007305775","https://openalex.org/W2023255055","https://openalex.org/W2026939372","https://openalex.org/W2032860541","https://openalex.org/W2044498526","https://openalex.org/W2047798722","https://openalex.org/W2048426973","https://openalex.org/W2051118703","https://openalex.org/W2055243355","https://openalex.org/W2060412909","https://openalex.org/W2063433330","https://openalex.org/W2075844027","https://openalex.org/W2077331070","https://openalex.org/W2079750606","https://openalex.org/W2085205517","https://openalex.org/W2085867854","https://openalex.org/W2089216636","https://openalex.org/W2090118995","https://openalex.org/W2092777410","https://openalex.org/W2101936201","https://openalex.org/W2104344723","https://openalex.org/W2112402070","https://openalex.org/W2116024161","https://openalex.org/W2119872662","https://openalex.org/W2121589257","https://openalex.org/W2132756654","https://openalex.org/W2136005699","https://openalex.org/W2137490288","https://openalex.org/W2144957010","https://openalex.org/W2150040586","https://openalex.org/W2157520165","https://openalex.org/W2158500249","https://openalex.org/W2159199618","https://openalex.org/W2164028234","https://openalex.org/W2167385968","https://openalex.org/W2188321714","https://openalex.org/W2196404391","https://openalex.org/W2205275114","https://openalex.org/W2276064775","https://openalex.org/W2323549833","https://openalex.org/W2329014569","https://openalex.org/W2329619445","https://openalex.org/W2335213589","https://openalex.org/W2402334444","https://openalex.org/W2412391671","https://openalex.org/W2431249095","https://openalex.org/W2497429959","https://openalex.org/W2517408203","https://openalex.org/W2963657529","https://openalex.org/W4206550307"],"related_works":["https://openalex.org/W2014919333","https://openalex.org/W649162683","https://openalex.org/W2523650139","https://openalex.org/W2234798964","https://openalex.org/W4214704136","https://openalex.org/W2032446250","https://openalex.org/W2608626399","https://openalex.org/W2972713634","https://openalex.org/W1998192315","https://openalex.org/W396360056"],"abstract_inverted_index":{"A":[0,59],"change":[1],"in":[2,17,153],"enzyme":[3,25],"activity":[4,26,79],"has":[5,73,104],"been":[6,74],"used":[7],"as":[8,86],"a":[9,87,105],"clinical":[10],"biomarker":[11],"for":[12,66],"diagnosis":[13],"and":[14,54,68,129,148],"is":[15,94],"useful":[16],"evaluating":[18],"patient":[19],"prognosis.":[20],"Current":[21],"laboratory":[22],"measurements":[23],"of":[24,30,38,77,80,91,142,150],"involve":[27],"multi-step":[28],"derivatization":[29,131],"the":[31,44,50,70,78,99],"reaction":[32,45,53,71],"products":[33],"followed":[34],"by":[35,47,118],"quantitative":[36],"analysis":[37],"these":[39],"derivatives.":[40],"This":[41,120],"study":[42],"simplified":[43],"systems":[46],"using":[48,61],"only":[49],"target":[51],"enzymatic":[52],"directly":[55],"detecting":[56],"its":[57],"product.":[58],"protocol":[60],"paper":[62],"spray":[63,103],"mass":[64],"spectrometry":[65],"identifying":[67],"quantifying":[69],"product":[72],"developed.":[75],"Evaluation":[76],"aspartate":[81],"aminotransferase":[82],"(AST)":[83],"was":[84],"chosen":[85],"proof-of-principle.":[88],"The":[89],"volume":[90],"sample":[92,127],"needed":[93],"greatly":[95],"reduced":[96],"compared":[97],"with":[98],"traditional":[100],"method.":[101],"Paper":[102],"desalting":[106],"effect":[107],"that":[108],"avoids":[109],"sprayer":[110],"clogging":[111],"problems":[112],"seen":[113],"when":[114],"examining":[115],"serum":[116],"samples":[117],"nanoESI.":[119],"very":[121],"simple":[122],"method":[123],"does":[124],"not":[125],"require":[126],"pretreatment":[128],"additional":[130],"reactions,":[132],"yet":[133],"it":[134],"gives":[135],"high":[136],"quality":[137],"kinetic":[138],"data,":[139],"excellent":[140],"limits":[141],"detection":[143],"(60":[144],"ppb":[145],"from":[146],"serum),":[147],"coefficients":[149],"variation":[151],"<10%":[152],"quantitation.":[154],"Graphical":[155],"Abstract":[156],"\u115f.":[157]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":9},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2017-02-10T00:00:00"}