{"id":"https://openalex.org/W2583484614","doi":null,"title":"MicroRNA-221 is involved in the regulation of osteoporosis through regulates RUNX2 protein expression and osteoblast differentiation.","display_name":"MicroRNA-221 is involved in the regulation of osteoporosis through regulates RUNX2 protein expression and osteoblast differentiation.","publication_year":2017,"publication_date":"2017-01-01","ids":{"openalex":"https://openalex.org/W2583484614","mag":"2583484614","pmid":"https://pubmed.ncbi.nlm.nih.gov/28123639","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5250709"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:5250709","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5250709","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Am J Transl Res","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5250709","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009978995","display_name":"Yinquan Zhang","orcid":"https://orcid.org/0000-0002-4760-013X","observed_orcids":["https://orcid.org/0000-0002-4760-013X"]},"institutions":[{"id":"https://openalex.org/I3923682","display_name":"Soochow University","ror":"https://ror.org/05t8y2r12","country_code":"CN","type":"education","lineage":["https://openalex.org/I3923682"]},{"id":"https://openalex.org/I4210153519","display_name":"First Affiliated Hospital of Soochow University","ror":"https://ror.org/051jg5p78","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210153519"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yinquan Zhang","raw_affiliation_strings":["Department of Orthopedics, The First Affiliated Hospital, Soochow University Suzhou 215006, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The First Affiliated Hospital, Soochow University Suzhou 215006, China","institution_ids":["https://openalex.org/I3923682","https://openalex.org/I4210153519"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075388362","display_name":"Yulei Gao","orcid":"https://orcid.org/0000-0003-3099-2963","observed_orcids":["https://orcid.org/0000-0003-3099-2963"]},"institutions":[{"id":"https://openalex.org/I177933477","display_name":"Second Military Medical University","ror":"https://ror.org/04tavpn47","country_code":"CN","type":"education","lineage":["https://openalex.org/I177933477"]},{"id":"https://openalex.org/I4210137389","display_name":"Eastern Hepatobiliary Surgery Hospital","ror":"https://ror.org/043sbvg03","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210137389"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yulei Gao","raw_affiliation_strings":["Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China","institution_ids":["https://openalex.org/I177933477","https://openalex.org/I4210137389"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101576688","display_name":"Lijun Cai","orcid":"https://orcid.org/0000-0002-7661-1524","observed_orcids":["https://orcid.org/0000-0002-7661-1524"]},"institutions":[{"id":"https://openalex.org/I3923682","display_name":"Soochow University","ror":"https://ror.org/05t8y2r12","country_code":"CN","type":"education","lineage":["https://openalex.org/I3923682"]},{"id":"https://openalex.org/I4210153519","display_name":"First Affiliated Hospital of Soochow University","ror":"https://ror.org/051jg5p78","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210153519"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lijun Cai","raw_affiliation_strings":["Department of Orthopedics, The First Affiliated Hospital, Soochow University Suzhou 215006, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The First Affiliated Hospital, Soochow University Suzhou 215006, China","institution_ids":["https://openalex.org/I3923682","https://openalex.org/I4210153519"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033539432","display_name":"Fengning Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177933477","display_name":"Second Military Medical University","ror":"https://ror.org/04tavpn47","country_code":"CN","type":"education","lineage":["https://openalex.org/I177933477"]},{"id":"https://openalex.org/I4210137389","display_name":"Eastern Hepatobiliary Surgery Hospital","ror":"https://ror.org/043sbvg03","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210137389"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Fengning Li","raw_affiliation_strings":["Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China","institution_ids":["https://openalex.org/I177933477","https://openalex.org/I4210137389"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014362654","display_name":"Yi Lou","orcid":"https://orcid.org/0000-0002-8964-6048","observed_orcids":["https://orcid.org/0000-0002-8964-6048"]},"institutions":[{"id":"https://openalex.org/I177933477","display_name":"Second Military Medical University","ror":"https://ror.org/04tavpn47","country_code":"CN","type":"education","lineage":["https://openalex.org/I177933477"]},{"id":"https://openalex.org/I4210137389","display_name":"Eastern Hepatobiliary Surgery Hospital","ror":"https://ror.org/043sbvg03","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210137389"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yi Lou","raw_affiliation_strings":["Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China","institution_ids":["https://openalex.org/I177933477","https://openalex.org/I4210137389"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102003904","display_name":"Ning Xu","orcid":"https://orcid.org/0000-0002-8166-0320","observed_orcids":["https://orcid.org/0000-0002-8166-0320"]},"institutions":[{"id":"https://openalex.org/I177933477","display_name":"Second Military Medical University","ror":"https://ror.org/04tavpn47","country_code":"CN","type":"education","lineage":["https://openalex.org/I177933477"]},{"id":"https://openalex.org/I4210137389","display_name":"Eastern Hepatobiliary Surgery Hospital","ror":"https://ror.org/043sbvg03","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210137389"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ning Xu","raw_affiliation_strings":["Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China","institution_ids":["https://openalex.org/I177933477","https://openalex.org/I4210137389"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Yifan Kang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177933477","display_name":"Second Military Medical University","ror":"https://ror.org/04tavpn47","country_code":"CN","type":"education","lineage":["https://openalex.org/I177933477"]},{"id":"https://openalex.org/I4210137389","display_name":"Eastern Hepatobiliary Surgery Hospital","ror":"https://ror.org/043sbvg03","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210137389"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yifan Kang","raw_affiliation_strings":["Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Third Affiliated Hospital, Second Military Medical University Shanghai 201805, China","institution_ids":["https://openalex.org/I177933477","https://openalex.org/I4210137389"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087302968","display_name":"Huilin Yang","orcid":"https://orcid.org/0000-0002-9459-7981","observed_orcids":["https://orcid.org/0000-0002-9459-7981","https://orcid.org/0000-0003-4637-5177"]},"institutions":[{"id":"https://openalex.org/I3923682","display_name":"Soochow University","ror":"https://ror.org/05t8y2r12","country_code":"CN","type":"education","lineage":["https://openalex.org/I3923682"]},{"id":"https://openalex.org/I4210153519","display_name":"First Affiliated Hospital of Soochow University","ror":"https://ror.org/051jg5p78","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210153519"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Huilin Yang","raw_affiliation_strings":["Department of Orthopedics, The First Affiliated Hospital, Soochow University Suzhou 215006, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The First Affiliated Hospital, Soochow University Suzhou 215006, China","institution_ids":["https://openalex.org/I3923682","https://openalex.org/I4210153519"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.3838,"has_fulltext":false,"cited_by_count":78,"citation_normalized_percentile":{"value":0.95909205,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":100},"biblio":{"volume":"9","issue":"1","first_page":"126","last_page":"135"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11219","display_name":"Bone Metabolism and Diseases","score":0.7645999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11219","display_name":"Bone Metabolism and Diseases","score":0.7645999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.2167000025510788,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10071","display_name":"Bone health and osteoporosis research","score":0.010700000450015068,"subfield":{"id":"https://openalex.org/subfields/2732","display_name":"Orthopedics and Sports Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mir-221","display_name":"miR-221","score":0.9269999861717224},{"id":"https://openalex.org/keywords/microrna","display_name":"microRNA","score":0.8429999947547913},{"id":"https://openalex.org/keywords/osteoporosis","display_name":"osteoporosis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/runx2","display_name":"Runx2","score":0.9490000009536743},{"id":"https://openalex.org/keywords/osteogenic-differentiation","display_name":"osteogenic differentiation","score":0.9990000128746033},{"id":"https://openalex.org/keywords/osteoporotic-fractures","display_name":"osteoporotic fractures","score":0.7049999833106995},{"id":"https://openalex.org/keywords/trabecular-bone","display_name":"trabecular bone","score":0.9039999842643738},{"id":"https://openalex.org/keywords/femoral-neck","display_name":"femoral neck","score":0.7250000238418579},{"id":"https://openalex.org/keywords/osteocalcin","display_name":"osteocalcin","score":0.871999979019165},{"id":"https://openalex.org/keywords/alkaline-phosphatase","display_name":"alkaline phosphatase","score":0.9769999980926514},{"id":"https://openalex.org/keywords/col1a1","display_name":"COL1A1","score":0.9660000205039978},{"id":"https://openalex.org/keywords/3-untranslated-region","display_name":"3' untranslated region","score":0.8870000243186951},{"id":"https://openalex.org/keywords/c2c12-myoblasts","display_name":"C2C12 myoblasts","score":0.7480000257492065},{"id":"https://openalex.org/keywords/osteoarthritis","display_name":"osteoarthritis","score":0.5860000252723694}],"concepts":[{"id":"https://openalex.org/C2776363554","wikidata":"https://www.wikidata.org/wiki/Q14907085","display_name":"RUNX2","level":4,"score":0.9283732175827026},{"id":"https://openalex.org/C2778260815","wikidata":"https://www.wikidata.org/wiki/Q917177","display_name":"Osteoblast","level":3,"score":0.8319351673126221},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.7099946737289429},{"id":"https://openalex.org/C131075544","wikidata":"https://www.wikidata.org/wiki/Q415863","display_name":"Osteocalcin","level":4,"score":0.6563401818275452},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.616226315498352},{"id":"https://openalex.org/C160160445","wikidata":"https://www.wikidata.org/wiki/Q24769688","display_name":"Alkaline phosphatase","level":3,"score":0.5950817465782166},{"id":"https://openalex.org/C2776541429","wikidata":"https://www.wikidata.org/wiki/Q165328","display_name":"Osteoporosis","level":2,"score":0.5311630368232727},{"id":"https://openalex.org/C30352789","wikidata":"https://www.wikidata.org/wiki/Q14903153","display_name":"Bone morphogenetic protein 2","level":3,"score":0.4327372610569},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4190792739391327},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.41637223958969116},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3976108133792877},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.39450231194496155},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3596322536468506},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3499016761779785},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.2731693685054779},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2144668996334076},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11515942215919495},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.05181679129600525},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:5250709","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5250709","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Am J Transl Res","raw_type":"Text"},{"id":"pmid:28123639","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28123639","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American journal of translational research","raw_type":"Journal Article"},{"id":"mag:2583484614","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28123639","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:5250709","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5250709","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Am J Transl Res","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9940000176429749,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W609404933","https://openalex.org/W1509760969","https://openalex.org/W1513823711","https://openalex.org/W1536196126","https://openalex.org/W1567938686","https://openalex.org/W1579931870","https://openalex.org/W1906353506","https://openalex.org/W1935346479","https://openalex.org/W1936986964","https://openalex.org/W1968385628","https://openalex.org/W1986086252","https://openalex.org/W1990477077","https://openalex.org/W1997812904","https://openalex.org/W2005323891","https://openalex.org/W2006950202","https://openalex.org/W2016529303","https://openalex.org/W2020911494","https://openalex.org/W2031731215","https://openalex.org/W2050521206","https://openalex.org/W2057881514","https://openalex.org/W2059130444","https://openalex.org/W2069811152","https://openalex.org/W2071435113","https://openalex.org/W2093252878","https://openalex.org/W2122225018","https://openalex.org/W2142820105","https://openalex.org/W2147358001","https://openalex.org/W2152631846","https://openalex.org/W2171574676","https://openalex.org/W2176312960","https://openalex.org/W2176882903","https://openalex.org/W4244095134"],"related_works":["https://openalex.org/W2997709422","https://openalex.org/W4386443050","https://openalex.org/W2965131251","https://openalex.org/W3028036412","https://openalex.org/W2998243442","https://openalex.org/W2900844825","https://openalex.org/W2990642247","https://openalex.org/W2793005699","https://openalex.org/W2982494782","https://openalex.org/W3133847243"],"abstract_inverted_index":{"INTRODUCTION:":[0],"MicroRNAs":[1],"(miRNAs)":[2],"has":[3],"emerged":[4],"as":[5,144],"important":[6,259],"factors":[7],"in":[8,21,58,90,115,125,137,140,191,261],"osteogenesis":[9],"and":[10,26,77,107,123,161,178,267],"chondrogenesis.":[11],"This":[12],"study":[13],"aimed":[14],"to":[15,47],"determine":[16],"whether":[17],"miR-221":[18,89,135,171,189,203,233,241,256],"is":[19],"involved":[20],"the":[22,59,116,141,147,173,188,192,230,243],"regulation":[23],"of":[24,61,88,130,134,151,170,175,187,196,219,226,232],"osteoporosis":[25,62,262],"its":[27],"underlying":[28],"mechanism.":[29],"METHODS:":[30],"Total":[31],"RNA":[32],"was":[33,70,80,93,113,215],"extracted":[34],"from":[35,41],"fresh":[36],"femoral":[37],"neck":[38],"trabecular":[39],"bone":[40],"women":[42],"undergoing":[43],"hip":[44],"replacement":[45],"due":[46],"either":[48],"osteoporotic":[49,117],"fracture":[50],"(OP":[51],"group,":[52,64],"n":[53,65],"=":[54,66],"12)":[55],"or":[56,97],"osteoarthritis":[57],"absence":[60],"(Control":[63],"12).":[67],"Gene":[68],"expression":[69,149,266],"quantified":[71],"using":[72,105],"TaqMan":[73],"quantitative":[74],"RT-PCR":[75],"assays":[76,211],"protein":[78],"production":[79],"determined":[81],"by":[82,95,146,246],"western":[83],"blot":[84,199],"analysis.":[85],"The":[86],"role":[87],"osteoblast":[91,153,235,244,268],"differentiation":[92,245],"identified":[94,104,183],"gain":[96],"loss":[98],"function":[99],"experiment.":[100],"MiRNA":[101],"targets":[102],"were":[103],"bioinformatics":[106],"luciferase":[108],"reporter":[109,210],"assay.":[110],"RESULTS:":[111],"MiR-221":[112],"down-regulated":[114],"samples":[118],"compared":[119],"with":[120],"non-osteoporotic":[121],"controls,":[122],"decreased":[124],"a":[126,138,216],"C2C12":[127],"cell":[128],"model":[129],"osteogenic":[131,142],"differentiation.":[132,269],"Overexpression":[133],"resulted":[136],"decrease":[139],"potential,":[143],"indicated":[145],"reduced":[148],"levels":[150],"key":[152],"markers,":[154],"including":[155],"osteocalcin":[156],"(OC),":[157],"alkaline":[158],"phosphatase":[159],"(ALP)":[160],"collagen,":[162],"type":[163],"I,":[164],"\u03b1":[165],"1":[166],"(COL1A1),":[167],"whereas":[168],"inhibition":[169],"promoted":[172],"activity":[174],"OC,":[176],"ALP":[177],"COL1A1.":[179],"Then":[180],"bioinformatic":[181],"analysis":[182,200],"potential":[184],"target":[185,218],"sites":[186],"located":[190],"3'":[193],"untranslated":[194],"regions":[195],"RUNX2.":[197,248],"Western":[198],"demonstrated":[201],"that":[202,213,224,240,255],"inhibited":[204],"RUNX2":[205,214,227,265],"gene":[206],"expression.":[207],"Furthermore,":[208],"dual-luciferase":[209],"confirmed":[212],"direct":[217],"miR-221.":[220],"Rescue":[221],"experiments":[222],"showed":[223],"overexpression":[225],"significantly":[228],"attenuated":[229],"effect":[231],"on":[234],"markers":[236],"providing":[237],"strong":[238],"evidence":[239],"mediated":[242],"targeting":[247],"CONCLUSIONS:":[249],"Taken":[250],"together,":[251],"these":[252],"data":[253],"implied":[254],"played":[257],"an":[258],"part":[260],"through":[263],"regulating":[264]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":10},{"year":2021,"cited_by_count":11},{"year":2020,"cited_by_count":17},{"year":2019,"cited_by_count":14},{"year":2018,"cited_by_count":10},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2017-02-10T00:00:00"}
