{"id":"https://openalex.org/W2582847639","doi":null,"title":"Regulation of the multi-functional protein YscU in assembly of the Yersinia type III secretion injectisome","display_name":"Regulation of the multi-functional protein YscU in assembly of the Yersinia type III secretion injectisome","publication_year":2017,"publication_date":"2017-01-01","ids":{"openalex":"https://openalex.org/W2582847639","mag":"2582847639"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:umu-130134","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-130134","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-130134","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089769946","display_name":"Ho Ngoc Hoang Oanh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ho Ngoc Hoang Oanh","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5089769946"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12232","display_name":"Yersinia bacterium, plague, ectoparasites research","score":0.8755000233650208,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12232","display_name":"Yersinia bacterium, plague, ectoparasites research","score":0.8755000233650208,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.05719999969005585,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.05119999870657921,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/yersinia-pseudotuberculosis","display_name":"Yersinia pseudotuberculosis","score":0.906000018119812},{"id":"https://openalex.org/keywords/type-iii-secretion-system","display_name":"type III secretion system","score":0.9340000152587891},{"id":"https://openalex.org/keywords/calcium-binding","display_name":"calcium binding","score":0.7300000190734863},{"id":"https://openalex.org/keywords/virulence-plasmid","display_name":"virulence plasmid","score":0.8820000290870667},{"id":"https://openalex.org/keywords/effector-proteins","display_name":"effector proteins","score":0.5979999899864197},{"id":"https://openalex.org/keywords/bacterial-inner-membrane","display_name":"bacterial inner membrane","score":0.44699999690055847}],"concepts":[{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.7164673805236816},{"id":"https://openalex.org/C130211564","wikidata":"https://www.wikidata.org/wiki/Q21199165","display_name":"Type three secretion system","level":4,"score":0.5581482648849487},{"id":"https://openalex.org/C2776255393","wikidata":"https://www.wikidata.org/wiki/Q132231","display_name":"Yersinia","level":3,"score":0.5343498587608337},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4659042954444885},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.45712369680404663},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3347429633140564},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.32676616311073303},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24242085218429565},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.22164687514305115},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13173002004623413},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1103176474571228},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.08826300501823425}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:umu-130134","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-130134","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:2582847639","doi":null,"is_oa":false,"landing_page_url":"http://umu.diva-portal.org/smash/record.jsf?pid=diva2%3A1064298","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:umu-130134","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-130134","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":121,"referenced_works":["https://openalex.org/W584762326","https://openalex.org/W659552522","https://openalex.org/W1217953477","https://openalex.org/W1495119128","https://openalex.org/W1498615754","https://openalex.org/W1499303660","https://openalex.org/W1515020238","https://openalex.org/W1518136555","https://openalex.org/W1524981529","https://openalex.org/W1536775120","https://openalex.org/W1555925483","https://openalex.org/W1567074356","https://openalex.org/W1643603004","https://openalex.org/W1769519545","https://openalex.org/W1846851238","https://openalex.org/W1878324752","https://openalex.org/W1915721517","https://openalex.org/W1916327110","https://openalex.org/W1917680451","https://openalex.org/W1931849326","https://openalex.org/W1965995996","https://openalex.org/W1968215209","https://openalex.org/W1975578094","https://openalex.org/W1976949015","https://openalex.org/W1978279776","https://openalex.org/W1978473203","https://openalex.org/W1978982822","https://openalex.org/W1980577924","https://openalex.org/W1982396425","https://openalex.org/W1988148633","https://openalex.org/W1992577725","https://openalex.org/W1995412269","https://openalex.org/W1995613309","https://openalex.org/W1997423062","https://openalex.org/W1997479358","https://openalex.org/W1999280598","https://openalex.org/W1999533958","https://openalex.org/W2005473844","https://openalex.org/W2006635484","https://openalex.org/W2008708467","https://openalex.org/W2017076970","https://openalex.org/W2017728221","https://openalex.org/W2018271515","https://openalex.org/W2019228627","https://openalex.org/W2020638348","https://openalex.org/W2021071631","https://openalex.org/W2025141560","https://openalex.org/W2025938842","https://openalex.org/W2026177652","https://openalex.org/W2026727321","https://openalex.org/W2026907657","https://openalex.org/W2030517878","https://openalex.org/W2037490797","https://openalex.org/W2038397435","https://openalex.org/W2040990166","https://openalex.org/W2042447268","https://openalex.org/W2043514764","https://openalex.org/W2047091751","https://openalex.org/W2050069092","https://openalex.org/W2051241165","https://openalex.org/W2052424490","https://openalex.org/W2053669884","https://openalex.org/W2067406019","https://openalex.org/W2069143195","https://openalex.org/W2069709525","https://openalex.org/W2070184351","https://openalex.org/W2071947177","https://openalex.org/W2075454119","https://openalex.org/W2076060493","https://openalex.org/W2078684251","https://openalex.org/W2081966923","https://openalex.org/W2084273798","https://openalex.org/W2084852164","https://openalex.org/W2087186922","https://openalex.org/W2088848851","https://openalex.org/W2095746140","https://openalex.org/W2102906662","https://openalex.org/W2104571730","https://openalex.org/W2106240534","https://openalex.org/W2108099417","https://openalex.org/W2110553890","https://openalex.org/W2111205680","https://openalex.org/W2111306697","https://openalex.org/W2116018449","https://openalex.org/W2117865915","https://openalex.org/W2118200955","https://openalex.org/W2118235207","https://openalex.org/W2121952882","https://openalex.org/W2125872855","https://openalex.org/W2128843644","https://openalex.org/W2130712055","https://openalex.org/W2131904845","https://openalex.org/W2132091434","https://openalex.org/W2132180320","https://openalex.org/W2132589339","https://openalex.org/W2133366275","https://openalex.org/W2134358906","https://openalex.org/W2137121488","https://openalex.org/W2141037335","https://openalex.org/W2141219835","https://openalex.org/W2141222798","https://openalex.org/W2141639341","https://openalex.org/W2144878722","https://openalex.org/W2145299907","https://openalex.org/W2149525061","https://openalex.org/W2149706359","https://openalex.org/W2152301430","https://openalex.org/W2153187042","https://openalex.org/W2154971985","https://openalex.org/W2155173544","https://openalex.org/W2158077160","https://openalex.org/W2158369156","https://openalex.org/W2164905805","https://openalex.org/W2167210281","https://openalex.org/W2169821755","https://openalex.org/W2169898067","https://openalex.org/W2179902466","https://openalex.org/W2268507808","https://openalex.org/W2416231187","https://openalex.org/W2468510943","https://openalex.org/W2570342360"],"related_works":["https://openalex.org/W7096156950","https://openalex.org/W1963510575","https://openalex.org/W7067639762","https://openalex.org/W3119239104","https://openalex.org/W2794377761","https://openalex.org/W2157735947","https://openalex.org/W2567542964","https://openalex.org/W2141037335","https://openalex.org/W2141219835","https://openalex.org/W2805072843"],"abstract_inverted_index":{"Yersinia":[0,56,108],"pseudotuberculosis":[1],"is":[2,46,70,82,109,150,242,253,313,320,442,451,460,541],"a":[3,16,22,47,73,114,133,142,164,232,298,337,438,443,492,546],"Gram-negative":[4,38],"zoonotic":[5],"pathogenic":[6,37,52,596],"bacterium":[7],"causing":[8],"gastroenteritis":[9],"in":[10,35,72,163,191,199,202,237,250,301,369,477,495],"human":[11],"and":[12,89,119,132,158,171,189,194,201,211,268,278,306,318,353,379,396,432,448,521,554,572],"animals.":[13],"It":[14],"shares":[15],"conserved":[17,143,461,475],"virulence":[18],"plasmid":[19],"encoding":[20],"for":[21,98,152,186,258,315,446,453],"needle-like":[23],"secretion":[24,29,43,159,188,217,249,317,344,368,584],"machinery,":[25],"or":[26],"type":[27,41],"III":[28,42],"system,":[30],"which":[31,138,149,241,526],"can":[32,488,505],"be":[33,61,474,489,516,603],"found":[34,412,514,535],"other":[36,328,478],"bacteria.":[39],"The":[40,78,102,146,223,327,509],"system":[44],"(T3SS)":[45],"macromolecular":[48],"assembly":[49,154],"that":[50,181,247,351,413,469,504,536,565],"enables":[51],"effector":[53],"proteins":[54],"(or":[55],"outer":[57],"proteins,":[58],"Yops)":[59],"to":[60,111,215,226,244,287,322,437,500,515,518,522,543,578,602],"transported":[62],"into":[63],"eukaryotic":[64],"host":[65,99],"cells.":[66],"This":[67,311],"export":[68],"machinery":[69],"assembled":[71],"highly":[74],"ordered":[75],"stepwise":[76],"mechanism.":[77],"activation":[79],"of":[80,91,107,123,155,160,220,271,342,377,394,400,408,425,464,471,486,570,588],"T3SS":[81,260,447,594],"also":[83,254,476],"dependent":[84],"on":[85,362,404],"calcium":[86,245],"concentration,":[87],"temperature,":[88,210],"pH":[90,212,261,276],"the":[92,153,156,255,259,289,343,397,405,414,422,457,484,502,537,567,589,598],"growth":[93,205],"media":[94,206],"as":[95,113,208,297,336,383,491,549],"mimic":[96,299],"factors":[97],"cell\u2019s":[100],"contact.":[101],"T3SS-associated":[103],"inner-membrane":[104],"protein,":[105],"YscU,":[106],"proposed":[110,286],"function":[112,335],"substrate":[115,329],"specificity":[116,330],"switch":[117,331],"protein":[118,257,272,332,539],"forms":[120],"basal":[121],"structure":[122,270],"T3SS.":[124],"YscU":[125,252,354,378,419,441,465,487,507,571],"has":[126,334,527],"four":[127],"\u03b1":[128],"helical":[129],"transmembrane":[130],"domain":[131,136],"soluble":[134],"cytoplasmic":[135],"YscUC":[137,182,221,227,319,427,449,544],"undergoes":[139],"auto-proteolysis":[140,147],"at":[141,359,429],"N\u2191PTH":[144],"motif.":[145],"process,":[148],"required":[151,452],"injectisome":[157],"Yops,":[161],"results":[162],"10-kDa":[165],"C-terminal":[166],"polypeptide":[167],"fragment,":[168],"denoted":[169],"YscUCC":[170],"6-kDa":[172],"N-terminal":[173],"fragment":[174],"YscUCN.":[175],"In":[176,280,372,586],"this":[177,373,482],"thesis,":[178],"we":[179,411,534,560],"showed":[180],"dissociation":[183,423,450,472,485,520],"was":[184,228,285,381,402,513],"important":[185,314,386],"Yops":[187,316,367,454,583],"resulted":[190],"unfolded":[192],"YscUCN":[193,290],"oligomeric":[195],"YscUCC.":[196],"By":[197],"combination":[198],"vivo":[200],"vitro":[203],"methods,":[204],"conditions":[207],"calcium,":[209],"were":[213,273,576],"indicated":[214],"control":[216],"by":[218,264,293,392],"regulation":[219],"dissociation.":[222,326,508],"calcium-binding":[224],"isotherm":[225],"fit":[229],"best":[230],"with":[231,388,545,551,582],"one-site":[233],"binding":[234,312,376,398,542],"model":[235,439],"resulting":[236],"Kd":[238,389],"800":[239],"\u00b5M,":[240],"identical":[243],"level":[246],"blocks":[248],"vivo.":[251],"key":[256],"dependence,":[262],"demonstrated":[263],"thermal":[265],"unfolding":[266],"profile":[267],"secondary":[269],"altered":[274],"between":[275,593],"7.4":[277],"6.0.":[279],"addition,":[281],"bacterial":[282,323,479],"inner":[283],"membrane":[284,324],"assist":[288],"folding,":[291],"monitored":[292],"using":[294],"lipid":[295],"bilayer":[296],"environment":[300],"nuclear":[302],"magnetic":[303],"resonance":[304],"(NMR)":[305],"circular":[307],"dichroism":[308],"(CD)":[309],"spectroscopy.":[310],"anchored":[321],"upon":[325],"YscP":[333,352,380,401,415],"\u201cmolecular":[338],"ruler\u201d":[339],"controlling":[340],"length":[341],"needle.":[345],"Previous":[346],"genetic":[347],"experiments":[348],"have":[349,561,579],"suggested":[350],"interact":[355],"physically,":[356],"when":[357],"mutation":[358],"defined":[360],"residues":[361],"yscU":[363],"(suppressor":[364],"mutants)":[365],"rescued":[366],"null-yscP":[370],"mutant.":[371],"research,":[374],"direct":[375],"proved":[382],"weak":[384],"but":[385],"interaction":[387,416],"430":[390],"mM":[391],"application":[393],"NMR":[395],"interface":[399],"centred":[403],"last":[406],"helix":[407],"YscUC.":[409],"Furthermore,":[410],"could":[417],"inhibit":[418,519],"auto-proteolysis.":[420],"Studying":[421],"kinetic":[424],"suppressor":[426],"variants":[428],"temperature":[430,444],"30":[431],"37oC":[433],"provides":[434],"strong":[435,590],"support":[436],"where":[440],"sensor":[445],"secretion.":[455],"Interestingly,":[456],"NPTH":[458],"motif":[459],"through":[462],"most":[463],"family":[466],"members,":[467],"meaning":[468],"role":[470],"may":[473],"injectisomes.":[480],"To":[481],"end,":[483],"used":[490],"therapeutic":[493],"target":[494],"drug":[496],"discovery.":[497],"We":[498],"attempted":[499],"identify":[501],"small-molecules":[503],"hinder":[506],"small":[510],"compound":[511],"methyl(5-methyl-2-phenyl-1,3-thiazolidin-4-yl)acetate":[512],"able":[517],"crystalize":[523],"full":[524],"YscUC,":[525],"never":[528],"been":[529],"successfully":[530],"done":[531],"before.":[532],"Finally,":[533],"inner-rod":[538],"YscI":[540],"1:1":[547],"stoichiometry":[548],"shown":[550,577],"pull-down":[552],"assays":[553],"isothermal":[555],"titration":[556],"calorimetry.":[557],"Taken":[558],"together":[559],"made":[562],"several":[563],"discoveries":[564],"expand":[566],"functional":[568],"palette":[569],"all":[573],"these":[574],"functions":[575],"biological":[580],"relevance":[581],"levels.":[585],"light":[587],"sequence":[591],"conservation":[592],"utilizing":[595],"bacteria":[597],"findings":[599],"are":[600],"likely":[601],"general":[604],"characters.":[605]},"counts_by_year":[],"updated_date":"2026-08-13T07:04:57.449891","created_date":"2017-02-03T00:00:00"}
