{"id":"https://openalex.org/W2581808342","doi":"https://doi.org/10.1039/c6cc10243c","title":"One-pot native chemical ligation by combination of two orthogonal thioester precursors","display_name":"One-pot native chemical ligation by combination of two orthogonal thioester precursors","publication_year":2017,"publication_date":"2017-01-01","ids":{"openalex":"https://openalex.org/W2581808342","doi":"https://doi.org/10.1039/c6cc10243c","mag":"2581808342","pmid":"https://pubmed.ncbi.nlm.nih.gov/28133659"},"language":"en","primary_location":{"id":"doi:10.1039/c6cc10243c","doi":"https://doi.org/10.1039/c6cc10243c","is_oa":false,"landing_page_url":"https://doi.org/10.1039/c6cc10243c","pdf_url":null,"source":{"id":"https://openalex.org/S152760256","display_name":"Chemical Communications","issn_l":"1359-7345","issn":["1359-7345","1364-548X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-2","ki-jl-1","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical Communications","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060880830","display_name":"Yuya Asahina","orcid":"https://orcid.org/0000-0001-6814-245X","observed_orcids":["https://orcid.org/0000-0001-6814-245X"]},"institutions":[{"id":"https://openalex.org/I4405286456","display_name":"Institute for Protein Research, The University of Osaka","ror":"https://ror.org/0268snt13","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286456","https://openalex.org/I98285908"]},{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuya Asahina","raw_affiliation_strings":["Institute for Protein Research","Japan","Osaka University","Suita","Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Protein Research","institution_ids":["https://openalex.org/I4405286456"]},{"raw_affiliation_string":"Japan","institution_ids":[]},{"raw_affiliation_string":"Osaka University","institution_ids":["https://openalex.org/I98285908"]},{"raw_affiliation_string":"Suita","institution_ids":[]},{"raw_affiliation_string":"Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I4405286456","https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015766818","display_name":"Toru Kawakami","orcid":"https://orcid.org/0000-0001-6439-7623","observed_orcids":["https://orcid.org/0000-0001-6439-7623"]},"institutions":[{"id":"https://openalex.org/I4405286456","display_name":"Institute for Protein Research, The University of Osaka","ror":"https://ror.org/0268snt13","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286456","https://openalex.org/I98285908"]},{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toru Kawakami","raw_affiliation_strings":["Institute for Protein Research","Japan","Osaka University","Suita","Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Protein Research","institution_ids":["https://openalex.org/I4405286456"]},{"raw_affiliation_string":"Japan","institution_ids":[]},{"raw_affiliation_string":"Osaka University","institution_ids":["https://openalex.org/I98285908"]},{"raw_affiliation_string":"Suita","institution_ids":[]},{"raw_affiliation_string":"Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I4405286456","https://openalex.org/I98285908"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5078558807","display_name":"Hironobu Hojo","orcid":"https://orcid.org/0000-0003-0916-1233","observed_orcids":["https://orcid.org/0000-0003-0916-1233"]},"institutions":[{"id":"https://openalex.org/I4405286456","display_name":"Institute for Protein Research, The University of Osaka","ror":"https://ror.org/0268snt13","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286456","https://openalex.org/I98285908"]},{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hironobu Hojo","raw_affiliation_strings":["Institute for Protein Research","Japan","Osaka University","Suita","Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan"],"raw_orcid":"https://orcid.org/0000-0003-0916-1233","affiliations":[{"raw_affiliation_string":"Institute for Protein Research","institution_ids":["https://openalex.org/I4405286456"]},{"raw_affiliation_string":"Japan","institution_ids":[]},{"raw_affiliation_string":"Osaka University","institution_ids":["https://openalex.org/I98285908"]},{"raw_affiliation_string":"Suita","institution_ids":[]},{"raw_affiliation_string":"Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I4405286456","https://openalex.org/I98285908"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5078558807"],"corresponding_institution_ids":["https://openalex.org/I4405286456","https://openalex.org/I98285908"],"apc_list":null,"apc_paid":null,"fwci":2.1194,"has_fulltext":false,"cited_by_count":37,"citation_normalized_percentile":{"value":0.86354,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"53","issue":"13","first_page":"2114","last_page":"2117"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/native-chemical-ligation","display_name":"native chemical ligation","score":0.9419999718666077},{"id":"https://openalex.org/keywords/protecting-groups","display_name":"protecting groups","score":0.7020000219345093},{"id":"https://openalex.org/keywords/aspartate","display_name":"aspartic acid","score":0.7300000190734863},{"id":"https://openalex.org/keywords/cysteine","display_name":"cysteine","score":0.9929999709129333},{"id":"https://openalex.org/keywords/polypeptide-synthesis","display_name":"polypeptide synthesis","score":0.8190000057220459}],"concepts":[{"id":"https://openalex.org/C2778834346","wikidata":"https://www.wikidata.org/wiki/Q422794","display_name":"Thioester","level":3,"score":0.9752510786056519},{"id":"https://openalex.org/C2778032875","wikidata":"https://www.wikidata.org/wiki/Q17152469","display_name":"Native chemical ligation","level":4,"score":0.9443936347961426},{"id":"https://openalex.org/C179437574","wikidata":"https://www.wikidata.org/wiki/Q15089798","display_name":"Ligation","level":2,"score":0.8769222497940063},{"id":"https://openalex.org/C99207889","wikidata":"https://www.wikidata.org/wiki/Q5090481","display_name":"Chemical ligation","level":3,"score":0.762853741645813},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.632316529750824},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.4835036098957062},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.47141262888908386},{"id":"https://openalex.org/C114373084","wikidata":"https://www.wikidata.org/wiki/Q273499","display_name":"Chemical synthesis","level":3,"score":0.3388795852661133},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27340763807296753},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.15798601508140564},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.13220176100730896},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.08295276761054993},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.06264364719390869}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1039/c6cc10243c","doi":"https://doi.org/10.1039/c6cc10243c","is_oa":false,"landing_page_url":"https://doi.org/10.1039/c6cc10243c","pdf_url":null,"source":{"id":"https://openalex.org/S152760256","display_name":"Chemical Communications","issn_l":"1359-7345","issn":["1359-7345","1364-548X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-2","ki-jl-1","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical Communications","raw_type":"journal-article"},{"id":"pmid:28133659","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28133659","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical communications (Cambridge, England)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G153443893","display_name":"Chemical synthesis of caveolin","funder_award_id":"16H04180","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G5316694757","display_name":"Synthetic study of the O-glycoprotein using the solubilizing method","funder_award_id":"15K21124","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G7652314343","display_name":"Synthesis of histone protein for the post-translationally modified protein library","funder_award_id":"15K05565","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1967274768","https://openalex.org/W1980102812","https://openalex.org/W1990555660","https://openalex.org/W2001566285","https://openalex.org/W2005446362","https://openalex.org/W2007708133","https://openalex.org/W2023096418","https://openalex.org/W2033721855","https://openalex.org/W2041084364","https://openalex.org/W2051709335","https://openalex.org/W2062929107","https://openalex.org/W2076635994","https://openalex.org/W2093610026","https://openalex.org/W2095321945","https://openalex.org/W2097299606","https://openalex.org/W2104516185","https://openalex.org/W2106972865","https://openalex.org/W2116179149","https://openalex.org/W2118134867","https://openalex.org/W2131314073","https://openalex.org/W2138995597","https://openalex.org/W2147276719","https://openalex.org/W2148405034","https://openalex.org/W2163197814","https://openalex.org/W2165805215","https://openalex.org/W2166405062","https://openalex.org/W2233916725","https://openalex.org/W2236410437","https://openalex.org/W2249507231","https://openalex.org/W2295920033","https://openalex.org/W2322721974","https://openalex.org/W2322965095","https://openalex.org/W2326360051","https://openalex.org/W2419352843","https://openalex.org/W2513948249","https://openalex.org/W4213262935"],"related_works":["https://openalex.org/W2513948249","https://openalex.org/W613635637","https://openalex.org/W7125017029","https://openalex.org/W2067584854","https://openalex.org/W2015586954","https://openalex.org/W1984574571","https://openalex.org/W2054012870","https://openalex.org/W569179959","https://openalex.org/W2587013592","https://openalex.org/W7212355532"],"abstract_inverted_index":{"We":[0],"developed":[1],"a":[2,13],"one-pot":[3,31],"peptide":[4],"ligation":[5,18],"method":[6,42],"using":[7],"two":[8,27],"orthogonal":[9],"thioester":[10],"precursors":[11,28],"and":[12,22,33],"protecting":[14],"group":[15],"for":[16],"the":[17,26,30,35,47],"reaction":[19],"between":[20],"Asp":[21],"Cys.":[23],"Combination":[24],"of":[25,40,50],"facilitated":[29],"operation":[32],"yielded":[34],"entire":[36],"polypeptide.":[37],"The":[38],"usefulness":[39],"this":[41],"was":[43],"successfully":[44],"demonstrated":[45],"by":[46],"total":[48],"synthesis":[49],"histone":[51],"H4.":[52]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":13},{"year":2017,"cited_by_count":2}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2017-02-03T00:00:00"}