{"id":"https://openalex.org/W2580939152","doi":"https://doi.org/10.1002/dvg.23013","title":"Probing the biology of cell boundary conditions through confinement of Xenopus cell\u2010free cytoplasmic extracts","display_name":"Probing the biology of cell boundary conditions through confinement of Xenopus cell\u2010free cytoplasmic extracts","publication_year":2017,"publication_date":"2017-01-01","ids":{"openalex":"https://openalex.org/W2580939152","doi":"https://doi.org/10.1002/dvg.23013","mag":"2580939152","pmid":"https://pubmed.ncbi.nlm.nih.gov/28132422","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5308884"},"language":"en","primary_location":{"id":"doi:10.1002/dvg.23013","doi":"https://doi.org/10.1002/dvg.23013","is_oa":false,"landing_page_url":"https://doi.org/10.1002/dvg.23013","pdf_url":null,"source":{"id":"https://openalex.org/S124648948","display_name":"genesis","issn_l":"1526-954X","issn":["1526-954X","1526-968X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"genesis","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5308884","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021876596","display_name":"Jessica G. Bermudez","orcid":"https://orcid.org/0000-0001-8622-677X","observed_orcids":["https://orcid.org/0000-0001-8622-677X"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jessica G. Bermudez","raw_affiliation_strings":["Department of Bioengineering, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","Authors contributed equally to this work"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Authors contributed equally to this work","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090031953","display_name":"Hui Chen","orcid":"https://orcid.org/0000-0002-8629-8834","observed_orcids":["https://orcid.org/0000-0002-8629-8834"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hui Chen","raw_affiliation_strings":["Department of Cell and Developmental Biology, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","Authors contributed equally to this work"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Developmental Biology, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Authors contributed equally to this work","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045612172","display_name":"Lily C. Einstein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lily C. Einstein","raw_affiliation_strings":["Department of Cell and Developmental Biology, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","Authors contributed equally to this work"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Developmental Biology, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Authors contributed equally to this work","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088939845","display_name":"Matthew C. Good","orcid":"https://orcid.org/0000-0002-6367-1034","observed_orcids":["https://orcid.org/0000-0002-6367-1034"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Matthew C. Good","raw_affiliation_strings":["Department of Bioengineering, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","Department of Cell and Developmental Biology, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","institution_ids":["https://openalex.org/I79576946"]},{"raw_affiliation_string":"Department of Cell and Developmental Biology, University of Pennsylvania, 421 Curie Blvd, 1151 BRB II/III, Philadelphia, Pennsylvania, 19104","institution_ids":["https://openalex.org/I79576946"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5088939845"],"corresponding_institution_ids":["https://openalex.org/I79576946"],"apc_list":{"value":3800,"currency":"USD","value_usd":3800},"apc_paid":null,"fwci":0.1274,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.06344697,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"55","issue":"1-2","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.6753000020980835,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.6753000020980835,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.11140000075101852,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11190","display_name":"3D Printing in Biomedical Research","score":0.031099999323487282,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/confinement","display_name":"confinement","score":0.8429999947547913},{"id":"https://openalex.org/keywords/microfluidics","display_name":"microfluidics","score":0.8809999823570251},{"id":"https://openalex.org/keywords/microfabrication","display_name":"microfabrication","score":0.9710000157356262},{"id":"https://openalex.org/keywords/intracellular-compartmentalization","display_name":"intracellular compartmentalization","score":0.6899999976158142}],"concepts":[{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.8856855630874634},{"id":"https://openalex.org/C2779535977","wikidata":"https://www.wikidata.org/wiki/Q1342298","display_name":"Xenopus","level":3,"score":0.8728911876678467},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6410791873931885},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.607964813709259},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.5886381268501282},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.4239829182624817},{"id":"https://openalex.org/C8673954","wikidata":"https://www.wikidata.org/wiki/Q138845","display_name":"Microfluidics","level":2,"score":0.41010090708732605},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3408384323120117},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13805419206619263},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.09986656904220581},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002457","descriptor_name":"Cell Extracts","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010063","descriptor_name":"Ovum","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010063","descriptor_name":"Ovum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014982","descriptor_name":"Xenopus laevis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D044085","descriptor_name":"Microfluidics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1002/dvg.23013","doi":"https://doi.org/10.1002/dvg.23013","is_oa":false,"landing_page_url":"https://doi.org/10.1002/dvg.23013","pdf_url":null,"source":{"id":"https://openalex.org/S124648948","display_name":"genesis","issn_l":"1526-954X","issn":["1526-954X","1526-968X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"genesis","raw_type":"journal-article"},{"id":"pmid:28132422","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28132422","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genesis (New York, N.Y. : 2000)","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:4661236","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5308884","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5308884","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5308884","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Genesis","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:4661236","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5308884","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Gender equality","id":"https://metadata.un.org/sdg/5","score":0.4699999988079071}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2673162305","display_name":"Training Program in Cell and Molecular Biology","funder_award_id":"T32GM007229","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306133","display_name":"Burroughs Wellcome Fund","ror":"https://ror.org/01d35cw23"},{"id":"https://openalex.org/F4320309370","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":108,"referenced_works":["https://openalex.org/W86335932","https://openalex.org/W149621205","https://openalex.org/W342736715","https://openalex.org/W935855719","https://openalex.org/W983771422","https://openalex.org/W1507864482","https://openalex.org/W1581941249","https://openalex.org/W1582860366","https://openalex.org/W1963969470","https://openalex.org/W1964253746","https://openalex.org/W1964726582","https://openalex.org/W1965792464","https://openalex.org/W1966099069","https://openalex.org/W1973513361","https://openalex.org/W1978583145","https://openalex.org/W1980858525","https://openalex.org/W1981233250","https://openalex.org/W1982409013","https://openalex.org/W1983821082","https://openalex.org/W1988260329","https://openalex.org/W1988573682","https://openalex.org/W1988852424","https://openalex.org/W1994150096","https://openalex.org/W1995841941","https://openalex.org/W1998485219","https://openalex.org/W1998664418","https://openalex.org/W2003997462","https://openalex.org/W2006320530","https://openalex.org/W2007368235","https://openalex.org/W2008954677","https://openalex.org/W2009911474","https://openalex.org/W2010399610","https://openalex.org/W2011947766","https://openalex.org/W2013405556","https://openalex.org/W2013775624","https://openalex.org/W2013801092","https://openalex.org/W2014494440","https://openalex.org/W2016098182","https://openalex.org/W2018317768","https://openalex.org/W2020652110","https://openalex.org/W2020762251","https://openalex.org/W2029225125","https://openalex.org/W2030104183","https://openalex.org/W2030923676","https://openalex.org/W2032532142","https://openalex.org/W2034373020","https://openalex.org/W2035835061","https://openalex.org/W2037450375","https://openalex.org/W2040656223","https://openalex.org/W2048990794","https://openalex.org/W2049617777","https://openalex.org/W2055330891","https://openalex.org/W2056707942","https://openalex.org/W2064062498","https://openalex.org/W2064479765","https://openalex.org/W2064585995","https://openalex.org/W2065790254","https://openalex.org/W2065868796","https://openalex.org/W2071129408","https://openalex.org/W2077813071","https://openalex.org/W2078768877","https://openalex.org/W2080002581","https://openalex.org/W2081446575","https://openalex.org/W2085381747","https://openalex.org/W2088617194","https://openalex.org/W2089685532","https://openalex.org/W2090690176","https://openalex.org/W2091992164","https://openalex.org/W2093026332","https://openalex.org/W2093291036","https://openalex.org/W2095734482","https://openalex.org/W2098623212","https://openalex.org/W2107332774","https://openalex.org/W2112481873","https://openalex.org/W2112940322","https://openalex.org/W2117279934","https://openalex.org/W2125900715","https://openalex.org/W2126955433","https://openalex.org/W2130059660","https://openalex.org/W2130229552","https://openalex.org/W2133492742","https://openalex.org/W2137034111","https://openalex.org/W2139869842","https://openalex.org/W2142021199","https://openalex.org/W2142423992","https://openalex.org/W2150463050","https://openalex.org/W2151729645","https://openalex.org/W2152969835","https://openalex.org/W2157253543","https://openalex.org/W2160594875","https://openalex.org/W2169171478","https://openalex.org/W2210663276","https://openalex.org/W2248624084","https://openalex.org/W2276448171","https://openalex.org/W2283383082","https://openalex.org/W2331797565","https://openalex.org/W2339521790","https://openalex.org/W2345428774","https://openalex.org/W2413649936","https://openalex.org/W2414057877","https://openalex.org/W2428231201","https://openalex.org/W2430303459","https://openalex.org/W2443474297","https://openalex.org/W2507375557","https://openalex.org/W2529600505","https://openalex.org/W4206957121","https://openalex.org/W4238701569","https://openalex.org/W4290260729"],"related_works":["https://openalex.org/W3112192482","https://openalex.org/W6987853099","https://openalex.org/W2786343555","https://openalex.org/W4396664148","https://openalex.org/W2428231201","https://openalex.org/W2079039506","https://openalex.org/W4380291234","https://openalex.org/W2912770194","https://openalex.org/W2017530995","https://openalex.org/W4416276309"],"abstract_inverted_index":{"Cell-free":[0],"cytoplasmic":[1],"extracts":[2],"prepared":[3],"from":[4],"Xenopus":[5,122],"eggs":[6],"and":[7,31,43,102,112,125,140],"embryos":[8],"have":[9,138],"for":[10,145],"decades":[11],"provided":[12],"a":[13,67,78,89,100],"biochemical":[14,63],"system":[15],"with":[16],"which":[17,83],"to":[18,128],"interrogate":[19],"complex":[20],"cell":[21],"biological":[22,135],"processes":[23],"in":[24,34,41,44,66,108],"vitro.":[25],"Recently,":[26],"the":[27,38,72,85,96,103,119,133,141],"application":[28],"of":[29,50,88,95,99,121],"microfabrication":[30],"microfluidic":[32],"strategies":[33],"biology":[35],"has":[36],"narrowed":[37],"gap":[39],"between":[40],"vitro":[42],"vivo":[45],"studies":[46],"by":[47],"enabling":[48],"formation":[49],"cell-size":[51],"compartments":[52],"containing":[53],"functional":[54],"cytoplasm.":[55],"These":[56],"approaches":[57],"provide":[58],"numerous":[59],"advantages":[60],"over":[61],"traditional":[62],"experiments":[64],"performed":[65],"test":[68],"tube.":[69],"Most":[70],"notably,":[71],"cell-free":[73,123],"cytoplasm":[74,124],"is":[75],"confined":[76],"using":[77],"two-":[79],"or":[80],"three-dimensional":[81],"boundary,":[82],"mimics":[84],"natural":[86],"configuration":[87],"cell.":[90],"This":[91],"strategy":[92],"enables":[93],"characterization":[94],"spatial":[97],"organization":[98],"cell,":[101],"role":[104],"that":[105],"boundaries":[106],"play":[107],"regulating":[109],"intracellular":[110],"assembly":[111],"function.":[113],"In":[114],"this":[115],"review,":[116],"we":[117],"describe":[118],"marriage":[120],"confinement":[126],"technologies":[127],"generate":[129],"synthetic":[130],"cell-like":[131],"systems,":[132],"recent":[134],"insights":[136],"they":[137,143],"enabled,":[139],"promise":[142],"hold":[144],"future":[146],"scientific":[147],"discovery.":[148]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2017-02-03T00:00:00"}
