{"id":"https://openalex.org/W2579676087","doi":"https://doi.org/10.1182/blood.v104.11.3800.3800","title":"High Molecular Weight Kininogen Fragments Stimulate the Secretion of Interleukin 1\u03b2 through the Urokinase-Type Plasminogen Activator Receptor (uPAR) and CD11b/CD18 (Mac-1) in Human Blood Mononuclear Cells.","display_name":"High Molecular Weight Kininogen Fragments Stimulate the Secretion of Interleukin 1\u03b2 through the Urokinase-Type Plasminogen Activator Receptor (uPAR) and CD11b/CD18 (Mac-1) in Human Blood Mononuclear Cells.","publication_year":2004,"publication_date":"2004-11-16","ids":{"openalex":"https://openalex.org/W2579676087","doi":"https://doi.org/10.1182/blood.v104.11.3800.3800","mag":"2579676087"},"language":"en","primary_location":{"id":"doi:10.1182/blood.v104.11.3800.3800","doi":"https://doi.org/10.1182/blood.v104.11.3800.3800","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v104.11.3800.3800","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027128400","display_name":"Mohammad M. Khan","orcid":"https://orcid.org/0000-0001-5973-447X","observed_orcids":["https://orcid.org/0000-0001-5973-447X"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Mohammad M. Khan","raw_affiliation_strings":["The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087529422","display_name":"Harlan N. Bradford","orcid":"https://orcid.org/0000-0001-5582-278X","observed_orcids":["https://orcid.org/0000-0001-5582-278X"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Harlan N. Bradford","raw_affiliation_strings":["The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009021123","display_name":"Irma Isordia\u2010Salas","orcid":"https://orcid.org/0000-0002-8986-2330","observed_orcids":["https://orcid.org/0000-0002-8986-2330"]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Irma Isordia-Salas","raw_affiliation_strings":["The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113466403","display_name":"Ricardo G. Espinola","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ricardo Espinola","raw_affiliation_strings":["The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5104652394","display_name":"Robert W. Colman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert W. Colman","raw_affiliation_strings":["The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5009021123","https://openalex.org/A5027128400","https://openalex.org/A5087529422"],"corresponding_institution_ids":["https://openalex.org/I84392919"],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":0.3698,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.70131541,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"104","issue":"11","first_page":"3800","last_page":"3800"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11917","display_name":"Coagulation, Bradykinin, Polyphosphates, and Angioedema","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11917","display_name":"Coagulation, Bradykinin, Polyphosphates, and Angioedema","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10993","display_name":"Complement system in diseases","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14165","display_name":"Inflammation biomarkers and pathways","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/high-molecular-weight-kininogen","display_name":"high molecular weight kininogen","score":0.9950000047683716},{"id":"https://openalex.org/keywords/interleukin-1beta-secretion","display_name":"interleukin-1\u03b2 secretion","score":0.8920000195503235},{"id":"https://openalex.org/keywords/urokinase-type-plasminogen-activator-receptor","display_name":"urokinase-type plasminogen activator receptor","score":0.9200000166893005},{"id":"https://openalex.org/keywords/mac-1","display_name":"Mac-1","score":0.8600000143051147},{"id":"https://openalex.org/keywords/cd11b-cd18","display_name":"CD11b/CD18","score":0.9950000047683716},{"id":"https://openalex.org/keywords/bradykinin","display_name":"bradykinin","score":0.6940000057220459},{"id":"https://openalex.org/keywords/kallikrein","display_name":"kallikrein","score":0.6570000052452087},{"id":"https://openalex.org/keywords/blood-mononuclear-cells","display_name":"blood mononuclear cells","score":0.7799999713897705},{"id":"https://openalex.org/keywords/pro-inflammatory-cytokines","display_name":"inflammatory cytokines","score":0.6169999837875366}],"concepts":[{"id":"https://openalex.org/C2781317560","wikidata":"https://www.wikidata.org/wiki/Q24721273","display_name":"High-molecular-weight kininogen","level":5,"score":0.7536793351173401},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6780110001564026},{"id":"https://openalex.org/C137061746","wikidata":"https://www.wikidata.org/wiki/Q736033","display_name":"Peripheral blood mononuclear cell","level":3,"score":0.5889209508895874},{"id":"https://openalex.org/C56219504","wikidata":"https://www.wikidata.org/wiki/Q7900869","display_name":"Urokinase receptor","level":3,"score":0.5154493451118469},{"id":"https://openalex.org/C2777902427","wikidata":"https://www.wikidata.org/wiki/Q6413860","display_name":"Kininogen","level":4,"score":0.4894489347934723},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4828703701496124},{"id":"https://openalex.org/C2779679481","wikidata":"https://www.wikidata.org/wiki/Q13581432","display_name":"Plasminogen activator","level":2,"score":0.4623286724090576},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3269338011741638},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.31462958455085754},{"id":"https://openalex.org/C2779591629","wikidata":"https://www.wikidata.org/wiki/Q24745328","display_name":"Bradykinin","level":3,"score":0.24911528825759888},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2432570457458496},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.1429891288280487},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.1304909586906433}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1182/blood.v104.11.3800.3800","doi":"https://doi.org/10.1182/blood.v104.11.3800.3800","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v104.11.3800.3800","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2108394146","https://openalex.org/W2524595689","https://openalex.org/W2070317575","https://openalex.org/W1984649993","https://openalex.org/W1674245232","https://openalex.org/W1541073910","https://openalex.org/W2171231906","https://openalex.org/W2602651911","https://openalex.org/W2082116758","https://openalex.org/W2064012368"],"abstract_inverted_index":{"Abstract":[0],"High":[1],"molecular":[2],"weight":[3],"kininogen":[4,100,336],"(HK)":[5],"is":[6,47,281],"known":[7,285],"to":[8,13,24,57,221,292,299,339],"bind":[9],"specifically":[10],"and":[11,23,72,104,127,197,211,251,272,296,311,317],"saturably":[12],"Mac-1":[14,42],"with":[15,26,41,63,151,226,283],"a":[16,27,58,93,105,297],"Kd":[17,28],"=":[18],"9\u201318":[19],"nM":[20,30,184],"for":[21,31,111,169,203,229],"neutrophils":[22],"uPAR":[25,44],"=30":[29],"endothelial":[32],"cells.":[33,352],"However,":[34],"the":[35,191,340],"functional":[36],"results":[37,333],"of":[38,53,65,82,107,190,205,217,219,342],"HK":[39,56,126,210,240,261,293,300],"interaction":[40],"or":[43,160,179],"on":[45],"leukocytes":[46],"not":[48],"fully":[49],"understood.":[50],"Kallikrein":[51],"cleavage":[52],"single":[54],"chain":[55,60],"two":[59],"form":[61],"(HKa)":[62],"release":[64,259,306],"bradykinin":[66],"(BK)":[67],"occurs":[68],"in":[69,117,321,329],"sepsis,":[70],"arthritis,":[71],"inflammatory":[73,83,343],"bowel":[74],"disease.":[75],"We":[76,97],"hypothesized":[77],"that":[78,314,335],"HKa":[79,128,243,264],"stimulates":[80],"secretion":[81,216,238],"cytokines.":[84],"Mononuclear":[85],"cells":[86,196,228],"were":[87,129,149,185],"isolated":[88],"from":[89,348],"normal":[90],"subjects":[91],"by":[92,112,208,239,242,245,248,252,260,263,266,269,273,277,307,345],"Histopaque":[94],"density":[95],"gradient.":[96],"have":[98],"expressed":[99],"domain":[101,108],"3":[102],"(D3)":[103],"fragment":[106],"3,":[109],"coded":[110],"exon":[113],"7,":[114],"E7P":[115],"(aaG235-Q292),":[116],"E.":[118],"Coli":[119],"as":[120],"glutathione":[121],"S-transferase":[122],"(GST)":[123],"fusion":[124],"proteins.":[125,131],"purified":[130],"GST":[132,180],"was":[133,201],"recombinant.":[134],"All":[135],"proteins":[136],"contained":[137],"<0.01":[138],"EU/ml":[139],"endotoxin.":[140],"For":[141],"all":[142,181,212,330],"experiments,":[143],"2":[144],"X":[145],"106/ml":[146],"mononuclear":[147,192,227,351],"cells/ml":[148],"preincubated":[150],"monoclonal":[152,289],"antibodies,":[153],"murine":[154],"IgG":[155,325],"(both":[156],"at":[157,172,182,232],"1.8":[158],"mM)":[159],"HANKS":[161],"buffer":[162],"containing":[163],"0.15":[164],"M":[165],"NaCl,":[166],"pH":[167],"7.4":[168],"30":[170,230],"minutes":[171,231],"37\u00b0C.":[173,233],"HK,":[174,308],"HKa,":[175,309],"GST-D3,":[176],"GST-E7P,":[177],"GST-D5":[178,253,274,310],"600":[183],"added.":[186],"Centrifugation":[187],"allowed":[188],"separation":[189],"cell":[193],"suspension":[194],"into":[195],"supernatant.":[198],"The":[199],"latter":[200],"used":[202],"assay":[204],"interleukin-1\u03b2":[206],"(IL-1\u03b2)":[207],"ELISA.":[209],"fragments":[213],"tested":[214],"stimulated":[215],"IL-1\u03b2":[218,237,258,305,347],"84.8":[220],"306.3":[222],"pg/ml":[223],"when":[224],"incubated":[225],"Anti-Mac-1":[234],"antibody":[235,256,290],"inhibited":[236,257,304],"100%,":[241],"89%,":[244],"GST-D3":[246,267,312],"78%,":[247],"GST-E7P":[249,270],"94%":[250],"98%.":[254],"Anti-uPAR":[255],"88%,":[262],"77%,":[265],"95%,":[268],"85%,":[271],"76%.":[275],"Inhibition":[276],"both":[278,303,315],"receptor":[279],"antibodies":[280],"consistent":[282],"their":[284],"complex":[286],"formation.":[287],"A":[288],"(mAb)":[291],"D5":[294,318],"(C11C1)":[295],"mAb":[298],"D3":[301,316],"(2B5)":[302],"indicate":[313,334],"are":[319],"important":[320],"cytokine":[322],"release.":[323],"Murine":[324],"gave":[326],"0%":[327],"inhibition":[328],"studies.":[331],"These":[332],"may":[337],"contribute":[338],"pathogenesis":[341],"diseases":[344],"releasing":[346],"human":[349],"blood":[350]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2017-01-26T00:00:00"}