{"id":"https://openalex.org/W2579353171","doi":"https://doi.org/10.1080/14756366.2016.1261131","title":"Purification and biochemical characterization of a novel thermostable serine alkaline protease from Aeribacillus pallidus C10: a potential additive for detergents","display_name":"Purification and biochemical characterization of a novel thermostable serine alkaline protease from Aeribacillus pallidus C10: a potential additive for detergents","publication_year":2017,"publication_date":"2017-01-01","ids":{"openalex":"https://openalex.org/W2579353171","doi":"https://doi.org/10.1080/14756366.2016.1261131","mag":"2579353171","pmid":"https://pubmed.ncbi.nlm.nih.gov/28097910","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6010106"},"language":"en","primary_location":{"id":"doi:10.1080/14756366.2016.1261131","doi":"https://doi.org/10.1080/14756366.2016.1261131","is_oa":true,"landing_page_url":"https://doi.org/10.1080/14756366.2016.1261131","pdf_url":"https://www.tandfonline.com/doi/pdf/10.1080/14756366.2016.1261131?needAccess=true","source":{"id":"https://openalex.org/S192603849","display_name":"Journal of Enzyme Inhibition and Medicinal Chemistry","issn_l":"1475-6366","issn":["1475-6366","1475-6374"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Enzyme Inhibition and Medicinal Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.tandfonline.com/doi/pdf/10.1080/14756366.2016.1261131?needAccess=true","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028630387","display_name":"Vildan Yildirim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Vildan Yildirim","raw_affiliation_strings":["Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;","institution_ids":["https://openalex.org/I64134780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050664649","display_name":"Mustafa \u00d6zkan Baltac\u0131","orcid":"https://orcid.org/0000-0003-4968-9016","observed_orcids":["https://orcid.org/0000-0003-4968-9016"]},"institutions":[{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Mustafa Ozkan Baltaci","raw_affiliation_strings":["Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;","institution_ids":["https://openalex.org/I64134780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079833596","display_name":"\u0130lknur \u00d6zgen\u00e7li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Ilknur Ozgencli","raw_affiliation_strings":["Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Faculty of Science, Ataturk University, Erzurum, Turkey;","institution_ids":["https://openalex.org/I64134780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061049724","display_name":"Melda \u015ei\u015fecio\u011flu","orcid":"https://orcid.org/0000-0002-1127-0570","observed_orcids":["https://orcid.org/0000-0002-1127-0570"]},"institutions":[{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":true,"raw_author_name":"Melda Sisecioglu","raw_affiliation_strings":["Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;"],"raw_orcid":"https://orcid.org/0000-0002-1127-0570","affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;","institution_ids":["https://openalex.org/I64134780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042954694","display_name":"Ahmet Ad\u0131g\u00fczel","orcid":"https://orcid.org/0000-0001-8848-6647","observed_orcids":["https://orcid.org/0000-0001-8848-6647","https://orcid.org/0009-0000-7137-0461"]},"institutions":[{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":true,"raw_author_name":"Ahmet Adiguzel","raw_affiliation_strings":["Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;"],"raw_orcid":"https://orcid.org/0000-0001-8848-6647","affiliations":[{"raw_affiliation_string":"Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, Erzurum, Turkey;","institution_ids":["https://openalex.org/I64134780"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016236324","display_name":"G\u00fcl\u015fah Ad\u0131g\u00fczel","orcid":"https://orcid.org/0000-0003-1207-6742","observed_orcids":["https://orcid.org/0000-0003-1207-6742"]},"institutions":[{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Gulsah Adiguzel","raw_affiliation_strings":["Department of Food Hygiene and Technology, Faculty of Veterinary, Ataturk University, Erzurum, Turkey"],"raw_orcid":"https://orcid.org/0000-0003-1207-6742","affiliations":[{"raw_affiliation_string":"Department of Food Hygiene and Technology, Faculty of Veterinary, Ataturk University, Erzurum, Turkey","institution_ids":["https://openalex.org/I64134780"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5042954694","https://openalex.org/A5061049724"],"corresponding_institution_ids":["https://openalex.org/I64134780"],"apc_list":{"value":1880,"currency":"GBP","value_usd":2306},"apc_paid":{"value":1880,"currency":"GBP","value_usd":2306},"fwci":5.6155,"has_fulltext":false,"cited_by_count":65,"citation_normalized_percentile":{"value":0.97205346,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":99},"biblio":{"volume":"32","issue":"1","first_page":"468","last_page":"477"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aeribacillus-pallidus","display_name":"Aeribacillus pallidus","score":0.9860000014305115},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.824999988079071},{"id":"https://openalex.org/keywords/detergent-additives","display_name":"detergent additives","score":0.7459999918937683},{"id":"https://openalex.org/keywords/enzyme-stability","display_name":"enzyme stability","score":0.6600000262260437},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.578000009059906},{"id":"https://openalex.org/keywords/alkaline-protease","display_name":"alkaline protease","score":0.45500001311302185},{"id":"https://openalex.org/keywords/organic-solvent-tolerance","display_name":"organic solvent tolerance","score":0.5479999780654907}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7948428392410278},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7522192001342773},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.6728382706642151},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6597813963890076},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.6267260313034058},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.5982111096382141},{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.5978830456733704},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.5600656270980835},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.5251783132553101},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.46984949707984924},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.45377427339553833},{"id":"https://openalex.org/C206967267","wikidata":"https://www.wikidata.org/wiki/Q905749","display_name":"Ion chromatography","level":2,"score":0.4315760135650635},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.43126699328422546},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.3890790343284607},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.08241969347000122}],"mesh":[{"descriptor_ui":"D001407","descriptor_name":"Bacillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D003902","descriptor_name":"Detergents","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010450","descriptor_name":"Endopeptidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012694","descriptor_name":"Serine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1080/14756366.2016.1261131","doi":"https://doi.org/10.1080/14756366.2016.1261131","is_oa":true,"landing_page_url":"https://doi.org/10.1080/14756366.2016.1261131","pdf_url":"https://www.tandfonline.com/doi/pdf/10.1080/14756366.2016.1261131?needAccess=true","source":{"id":"https://openalex.org/S192603849","display_name":"Journal of Enzyme Inhibition and Medicinal Chemistry","issn_l":"1475-6366","issn":["1475-6366","1475-6374"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Enzyme Inhibition and Medicinal Chemistry","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:4992284","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6010106","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:6010106","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6010106","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Enzyme Inhib Med Chem","raw_type":"Text"},{"id":"pmid:28097910","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28097910","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of enzyme inhibition and medicinal chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:7af0c70e325147e9981b250b25825023","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/7af0c70e325147e9981b250b25825023","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Enzyme Inhibition and Medicinal Chemistry, Vol 32, Iss 1, Pp 468-477 (2017)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1080/14756366.2016.1261131","doi":"https://doi.org/10.1080/14756366.2016.1261131","is_oa":true,"landing_page_url":"https://doi.org/10.1080/14756366.2016.1261131","pdf_url":"https://www.tandfonline.com/doi/pdf/10.1080/14756366.2016.1261131?needAccess=true","source":{"id":"https://openalex.org/S192603849","display_name":"Journal of Enzyme Inhibition and Medicinal Chemistry","issn_l":"1475-6366","issn":["1475-6366","1475-6374"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Enzyme Inhibition and Medicinal Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2579353171.pdf"},"referenced_works_count":55,"referenced_works":["https://openalex.org/W934583181","https://openalex.org/W1570920124","https://openalex.org/W1755403693","https://openalex.org/W1841605125","https://openalex.org/W1900346143","https://openalex.org/W1964179770","https://openalex.org/W1967724527","https://openalex.org/W1974103341","https://openalex.org/W1974238570","https://openalex.org/W1976039532","https://openalex.org/W1976639063","https://openalex.org/W1978710967","https://openalex.org/W1978787477","https://openalex.org/W1980376684","https://openalex.org/W1992259028","https://openalex.org/W1994812687","https://openalex.org/W1996936181","https://openalex.org/W1997835504","https://openalex.org/W1998643089","https://openalex.org/W1999323561","https://openalex.org/W1999581248","https://openalex.org/W2003302033","https://openalex.org/W2005108404","https://openalex.org/W2007795720","https://openalex.org/W2009132407","https://openalex.org/W2012014784","https://openalex.org/W2018289835","https://openalex.org/W2027345286","https://openalex.org/W2032924530","https://openalex.org/W2034310782","https://openalex.org/W2038913723","https://openalex.org/W2041161646","https://openalex.org/W2043685439","https://openalex.org/W2048327124","https://openalex.org/W2050790746","https://openalex.org/W2053212327","https://openalex.org/W2058584290","https://openalex.org/W2061583103","https://openalex.org/W2068788016","https://openalex.org/W2073600578","https://openalex.org/W2075051942","https://openalex.org/W2076109478","https://openalex.org/W2093900229","https://openalex.org/W2100837269","https://openalex.org/W2102628698","https://openalex.org/W2112112542","https://openalex.org/W2128635872","https://openalex.org/W2149573662","https://openalex.org/W2151787491","https://openalex.org/W2181578141","https://openalex.org/W2183523049","https://openalex.org/W2342001225","https://openalex.org/W2460733747","https://openalex.org/W2463843056","https://openalex.org/W4237491994"],"related_works":["https://openalex.org/W2184097839","https://openalex.org/W2064908976","https://openalex.org/W2085074262","https://openalex.org/W2979795427","https://openalex.org/W2137616727","https://openalex.org/W2186330158","https://openalex.org/W952281315","https://openalex.org/W1999581248","https://openalex.org/W4400585134","https://openalex.org/W2915949602"],"abstract_inverted_index":{"An":[0],"extracellular":[1],"thermostable":[2],"alkaline":[3],"serine":[4],"protease":[5],"enzyme":[6,41,56,73,102,130],"from":[7],"Aeribacillus":[8],"pallidus":[9],"C10":[10],"(GenBank":[11],"No:":[12],"KC333049),":[13],"was":[14,42,69,104,126],"purified":[15],"4.85":[16],"and":[17,25,32,63,83,111,119,141,149],"17.":[18],"32-fold":[19],"with":[20,51],"a":[21,95],"yield":[22],"of":[23,39,80,91,116,137],"26.9":[24],"19.56%,":[26],"respectively,":[27],"through":[28,44],"DE52":[29],"anion":[30],"exchange":[31],"Probond":[33],"affinity":[34],"chromatography.":[35],"The":[36,55,101],"molecular":[37],"mass":[38],"the":[40,72,78,114,129,135],"determined":[43,70],"sodium":[45],"dodecyl":[46],"sulfate-polyacrylamide":[47],"gel":[48],"electrophoresis":[49],"(SDS-PAGE),":[50],"approximately":[52],"38.35":[53],"kDa.":[54],"exhibited":[57],"optimum":[58],"activity":[59,93,103,131],"at":[60,64,77,94],"pH":[61,81],"9":[62],"temperature":[65,97],"60":[66],"\u00b0C.":[67],"It":[68],"that":[71,84,128],"had":[74,86,132],"remained":[75],"stable":[76],"range":[79,98],"7.0\u201310.0,":[82],"it":[85,125],"preserved":[87],"more":[88,108],"than":[89,109],"80%":[90],"its":[92],"broad":[96],"(20\u201380":[99],"\u00b0C).":[100],"found":[105],"to":[106],"retain":[107],"70%":[110],"55%":[112],"in":[113,134],"presence":[115,136],"organic":[117],"solvents":[118],"commercial":[120],"detergents,":[121],"respectively.":[122,152],"In":[123],"addition,":[124],"observed":[127],"increased":[133],"5%":[138],"SDS.":[139],"KM":[140],"Vmax":[142],"values":[143],"were":[144],"calculated":[145],"as":[146],"0.197":[147],"mg/mL":[148],"7.29":[150],"\u03bcmol.mL\u22121.min\u22121,":[151]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":10},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":10},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":10},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2017-01-26T00:00:00"}