{"id":"https://openalex.org/W2576215656","doi":"https://doi.org/10.1007/s00253-016-8065-8","title":"McrA primers for the detection and quantification of the anaerobic archaeal methanotroph \u2018Candidatus Methanoperedens nitroreducens\u2019","display_name":"McrA primers for the detection and quantification of the anaerobic archaeal methanotroph \u2018Candidatus Methanoperedens nitroreducens\u2019","publication_year":2017,"publication_date":"2017-01-13","ids":{"openalex":"https://openalex.org/W2576215656","doi":"https://doi.org/10.1007/s00253-016-8065-8","mag":"2576215656","pmid":"https://pubmed.ncbi.nlm.nih.gov/28084539","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5266762"},"language":"en","primary_location":{"id":"doi:10.1007/s00253-016-8065-8","doi":"https://doi.org/10.1007/s00253-016-8065-8","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-016-8065-8","pdf_url":"https://link.springer.com/content/pdf/10.1007%2Fs00253-016-8065-8.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://link.springer.com/content/pdf/10.1007%2Fs00253-016-8065-8.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Annika Vaksmaa","orcid":"https://orcid.org/0000-0001-8794-8942","observed_orcids":["https://orcid.org/0000-0001-8794-8942"]},"institutions":[{"id":"https://openalex.org/I145872427","display_name":"Radboud University Nijmegen","ror":"https://ror.org/016xsfp80","country_code":"NL","type":"education","lineage":["https://openalex.org/I145872427"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"Annika Vaksmaa","raw_affiliation_strings":["Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands. avaksmaa@science.ru.nl","Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands"],"raw_orcid":"https://orcid.org/0000-0001-8794-8942","affiliations":[{"raw_affiliation_string":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands. avaksmaa@science.ru.nl","institution_ids":["https://openalex.org/I145872427"]},{"raw_affiliation_string":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I145872427"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036215557","display_name":"Mike S. M. Jetten","orcid":"https://orcid.org/0000-0002-4691-7039","observed_orcids":["https://orcid.org/0000-0002-4691-7039"]},"institutions":[{"id":"https://openalex.org/I145872427","display_name":"Radboud University Nijmegen","ror":"https://ror.org/016xsfp80","country_code":"NL","type":"education","lineage":["https://openalex.org/I145872427"]},{"id":"https://openalex.org/I4405281572","display_name":"Soehngen Institute of Anaerobic Microbiology","ror":"https://ror.org/04jdakz67","country_code":"NL","type":"funder","lineage":["https://openalex.org/I4405281572"]},{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Mike S. M. Jetten","raw_affiliation_strings":["Department of Biotechnology, Delft University of Technology, Delft, The Netherlands","Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands","Soehngen Institute of Anaerobic Microbiology, Nijmegen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Delft University of Technology, Delft, The Netherlands","institution_ids":["https://openalex.org/I98358874"]},{"raw_affiliation_string":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I145872427"]},{"raw_affiliation_string":"Soehngen Institute of Anaerobic Microbiology, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I4405281572"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011542237","display_name":"Katharina F. Ettwig","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145872427","display_name":"Radboud University Nijmegen","ror":"https://ror.org/016xsfp80","country_code":"NL","type":"education","lineage":["https://openalex.org/I145872427"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Katharina F. Ettwig","raw_affiliation_strings":["Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I145872427"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006443616","display_name":"Claudia L\u00fcke","orcid":"https://orcid.org/0000-0002-2502-5429","observed_orcids":["https://orcid.org/0000-0002-2502-5429"]},"institutions":[{"id":"https://openalex.org/I145872427","display_name":"Radboud University Nijmegen","ror":"https://ror.org/016xsfp80","country_code":"NL","type":"education","lineage":["https://openalex.org/I145872427"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"Claudia L\u00fcke","raw_affiliation_strings":["Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands. clueke@science.ru.nl","Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands. clueke@science.ru.nl","institution_ids":["https://openalex.org/I145872427"]},{"raw_affiliation_string":"Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I145872427"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5006443616"],"corresponding_institution_ids":["https://openalex.org/I145872427"],"apc_list":{"value":4190,"currency":"USD","value_usd":4190},"apc_paid":{"value":4190,"currency":"USD","value_usd":4190},"fwci":3.762,"has_fulltext":true,"cited_by_count":107,"citation_normalized_percentile":{"value":0.93690665,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":100},"biblio":{"volume":"101","issue":"4","first_page":"1631","last_page":"1641"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10995","display_name":"Methane Hydrates and Related Phenomena","score":0.8788999915122986,"subfield":{"id":"https://openalex.org/subfields/2304","display_name":"Environmental Chemistry"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10995","display_name":"Methane Hydrates and Related Phenomena","score":0.8788999915122986,"subfield":{"id":"https://openalex.org/subfields/2304","display_name":"Environmental Chemistry"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12688","display_name":"Microbial metabolism and enzyme function","score":0.06129999831318855,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.013000000268220901,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mcra-gene","display_name":"mcrA gene","score":0.7789999842643738},{"id":"https://openalex.org/keywords/methyl-coenzyme-m-reductase","display_name":"methyl-coenzyme M reductase","score":0.9520000219345093},{"id":"https://openalex.org/keywords/anaerobic-oxidation-of-methane","display_name":"anaerobic oxidation of methane","score":0.8889999985694885},{"id":"https://openalex.org/keywords/archaea","display_name":"archaea","score":0.6679999828338623},{"id":"https://openalex.org/keywords/qpcr-primer-design","display_name":"qPCR primer design","score":0.8370000123977661},{"id":"https://openalex.org/keywords/gene-quantification","display_name":"gene quantification","score":0.574999988079071},{"id":"https://openalex.org/keywords/phylogenetic-analysis","display_name":"phylogenetic analysis","score":0.7829999923706055},{"id":"https://openalex.org/keywords/paddy-soil","display_name":"paddy soil","score":0.8289999961853027},{"id":"https://openalex.org/keywords/river-sediments","display_name":"river sediments","score":0.8790000081062317},{"id":"https://openalex.org/keywords/marine-sediments","display_name":"marine sediment","score":0.9279999732971191},{"id":"https://openalex.org/keywords/nitrogen-cycle","display_name":"nitrogen cycling","score":0.5410000085830688},{"id":"https://openalex.org/keywords/methane-cycling","display_name":"methane cycling","score":0.7979999780654907}],"concepts":[{"id":"https://openalex.org/C2780147307","wikidata":"https://www.wikidata.org/wiki/Q857968","display_name":"Candidatus","level":4,"score":0.8484123349189758},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7410004734992981},{"id":"https://openalex.org/C550995028","wikidata":"https://www.wikidata.org/wiki/Q10872","display_name":"Archaea","level":3,"score":0.5840063691139221},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.5815054774284363},{"id":"https://openalex.org/C193252679","wikidata":"https://www.wikidata.org/wiki/Q242125","display_name":"Phylogenetic tree","level":3,"score":0.4814983606338501},{"id":"https://openalex.org/C2777438225","wikidata":"https://www.wikidata.org/wiki/Q1169785","display_name":"Methanogen","level":3,"score":0.4397856593132019},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.4200245141983032},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.35720834136009216},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.26712071895599365},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18769577145576477},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1147477924823761},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001105","descriptor_name":"Archaea","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001105","descriptor_name":"Archaea","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008697","descriptor_name":"Methane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":7,"locations":[{"id":"doi:10.1007/s00253-016-8065-8","doi":"https://doi.org/10.1007/s00253-016-8065-8","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-016-8065-8","pdf_url":"https://link.springer.com/content/pdf/10.1007%2Fs00253-016-8065-8.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},{"id":"pmid:28084539","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28084539","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied microbiology and biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:5266762","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5266762/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Microbiol Biotechnol","raw_type":"Text"},{"id":"pmh:ru:oai:repository.ubn.ru.nl:2066/168975","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2066/168975","pdf_url":"http://hdl.handle.net/2066/168975","source":{"id":"https://openalex.org/S4306401067","display_name":"Radboud Repository (Radboud University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I145872427","host_organization_name":"Radboud University Nijmegen","host_organization_lineage":["https://openalex.org/I145872427"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Applied Microbiology and Biotechnology, 101, 1631 - 1641","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:tud:oai:tudelft.nl:uuid:491127c5-2c9c-44da-a7b1-bc491292e1ed","doi":null,"is_oa":true,"landing_page_url":"http://resolver.tudelft.nl/uuid:491127c5-2c9c-44da-a7b1-bc491292e1ed","pdf_url":"http://resolver.tudelft.nl/uuid:491127c5-2c9c-44da-a7b1-bc491292e1ed","source":{"id":"https://openalex.org/S4306401843","display_name":"Data Archiving and Networked Services (DANS)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1322597698","host_organization_name":"Royal Netherlands Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/I1322597698"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Applied Microbiology and Biotechnology, 101(4)","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:repository.ubn.ru.nl:2066/168975","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/2066/168975","pdf_url":null,"source":{"id":"https://openalex.org/S4306401067","display_name":"Radboud Repository (Radboud University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I145872427","host_organization_name":"Radboud University Nijmegen","host_organization_lineage":["https://openalex.org/I145872427"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Applied Microbiology and Biotechnology, 101, 4, pp. 1631-1641","raw_type":"Article / Letter to editor"},{"id":"pmh:oai:tudelft.nl:uuid:491127c5-2c9c-44da-a7b1-bc491292e1ed","doi":null,"is_oa":true,"landing_page_url":"http://resolver.tudelft.nl/uuid:491127c5-2c9c-44da-a7b1-bc491292e1ed","pdf_url":null,"source":{"id":"https://openalex.org/S4306400906","display_name":"Research Repository (Delft University of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I98358874","host_organization_name":"Delft University of Technology","host_organization_lineage":["https://openalex.org/I98358874"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"journal article"}],"best_oa_location":{"id":"doi:10.1007/s00253-016-8065-8","doi":"https://doi.org/10.1007/s00253-016-8065-8","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s00253-016-8065-8","pdf_url":"https://link.springer.com/content/pdf/10.1007%2Fs00253-016-8065-8.pdf","source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1433606067","display_name":null,"funder_award_id":"024.002.002","funder_id":"https://openalex.org/F4320321800","funder_display_name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek"},{"id":"https://openalex.org/G3255201259","display_name":null,"funder_award_id":"024.002.001","funder_id":"https://openalex.org/F4320321800","funder_display_name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek"},{"id":"https://openalex.org/G5309730794","display_name":null,"funder_award_id":"VENI 863.13.007","funder_id":"https://openalex.org/F4320321800","funder_display_name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek"},{"id":"https://openalex.org/G7192596761","display_name":null,"funder_award_id":"ERC AG 339880 Eco_MoM","funder_id":"https://openalex.org/F4320334678","funder_display_name":"European Research Council"}],"funders":[{"id":"https://openalex.org/F4320317292","display_name":"Soehngen Institute of Anaerobic Microbiology","ror":"https://ror.org/04jdakz67"},{"id":"https://openalex.org/F4320321013","display_name":"Radboud Universiteit","ror":"https://ror.org/016xsfp80"},{"id":"https://openalex.org/F4320321800","display_name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","ror":"https://ror.org/04jsz6e67"},{"id":"https://openalex.org/F4320334678","display_name":"European Research Council","ror":"https://ror.org/0472cxd90"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2576215656.pdf","grobid_xml":"https://content.openalex.org/works/W2576215656.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W75231382","https://openalex.org/W1141018658","https://openalex.org/W1514390280","https://openalex.org/W1699172361","https://openalex.org/W1797923260","https://openalex.org/W1799118215","https://openalex.org/W1850431893","https://openalex.org/W1999165951","https://openalex.org/W2005549584","https://openalex.org/W2006162922","https://openalex.org/W2007720250","https://openalex.org/W2016407585","https://openalex.org/W2018180327","https://openalex.org/W2025383955","https://openalex.org/W2034285706","https://openalex.org/W2035339677","https://openalex.org/W2037480300","https://openalex.org/W2043551521","https://openalex.org/W2048644295","https://openalex.org/W2050686219","https://openalex.org/W2055043387","https://openalex.org/W2056257661","https://openalex.org/W2059860692","https://openalex.org/W2061105842","https://openalex.org/W2062046729","https://openalex.org/W2063563972","https://openalex.org/W2065104761","https://openalex.org/W2066959807","https://openalex.org/W2070868107","https://openalex.org/W2084251919","https://openalex.org/W2089465072","https://openalex.org/W2089969224","https://openalex.org/W2096485515","https://openalex.org/W2114831404","https://openalex.org/W2123927178","https://openalex.org/W2130485933","https://openalex.org/W2135784927","https://openalex.org/W2145778614","https://openalex.org/W2147818114","https://openalex.org/W2155806125","https://openalex.org/W2157953940","https://openalex.org/W2159408594","https://openalex.org/W2162017063","https://openalex.org/W2166536235","https://openalex.org/W2170270191","https://openalex.org/W2223979792","https://openalex.org/W2278367736","https://openalex.org/W2293468919","https://openalex.org/W2510258028","https://openalex.org/W2528367698","https://openalex.org/W2544513003","https://openalex.org/W4242729757"],"related_works":["https://openalex.org/W2804910636","https://openalex.org/W7142811738","https://openalex.org/W2747261137","https://openalex.org/W2742643885","https://openalex.org/W4315779426","https://openalex.org/W2982075883","https://openalex.org/W2653798136","https://openalex.org/W2017505825","https://openalex.org/W2070868107","https://openalex.org/W1482308130"],"abstract_inverted_index":{"The":[0,77,146],"nitrogen":[1],"and":[2,25,111,131,265,274],"methane":[3,21],"cycles":[4],"are":[5,42],"important":[6,284],"biogeochemical":[7],"processes.":[8],"Recently,":[9],"\u2018":[10,38,73,92,157,253,301],"Candidatus":[11,39,74,93,158,254,302],"Methanoperedens":[12],"nitroreducens,\u2019":[13],"archaea":[14],"that":[15,57,86,106,150,231],"catalyze":[16],"nitrate-dependent":[17,285],"anaerobic":[18],"oxidation":[19],"of":[20,37,51,63,72,84,153,155,210,249,267,277,282,300],"(AOM),":[22],"were":[23,28,123,134,204],"enriched,":[24],"their":[26],"genomes":[27],"analyzed.":[29],"Diagnostic":[30],"molecular":[31],"tools":[32],"for":[33,114,141,271],"the":[34,49,60,64,98,126,132,142,151,156,211,232,239,263,268,272,278,289],"sensitive":[35,275],"detection":[36,276],"M.":[40,75,94,159,255,303],"nitroreducens\u2019":[41,95,160,256,304],"not":[43],"yet":[44],"available.":[45],"Here,":[46],"we":[47],"report":[48],"design":[50],"two":[52],"novel":[53],"mcrA":[54,69,161,235,250,279,292,315],"primer":[55,79,100,129],"combinations":[56],"specifically":[58],"target":[59],"alpha":[61],"sub-unit":[62],"methyl-coenzyme":[65],"M":[66],"reductase":[67],"(":[68],")":[70],"gene":[71,162,236,280],"nitroreducens\u2019.":[76],"first":[78],"pair":[80,101],"produces":[81],"a":[82],"fragment":[83],"186-bp":[85],"can":[87],"be":[88],"used":[89],"to":[90,200,296],"quantify":[91],"cells,":[96],"whereas":[97,181],"second":[99],"yields":[102],"an":[103],"1191-bp":[104,234],"amplicon":[105],"is":[107],"with":[108,125,136,169,245],"sufficient":[109],"length":[110],"well":[112],"suited":[113],"more":[115],"detailed":[116],"phylogenetic":[117,298],"analyses.":[118],"Six":[119],"different":[120,240],"environmental":[121],"samples":[122],"evaluated":[124],"new":[127,269],"qPCR":[128,147],"pair,":[130],"abundances":[133],"compared":[135],"those":[137],"determined":[138],"using":[139],"primers":[140,270],"16S":[143],"rRNA":[144],"gene.":[145],"results":[148,261],"indicated":[149],"number":[152],"copies":[154,176,193,223],"was":[163],"highest":[164],"in":[165,207],"rice":[166],"field":[167],"soil,":[168],"5.6":[170],"\u00b1":[171,188,218],"0.8":[172],"\u00d7":[173,190,220],"10":[174,191,221],"6":[175],"g":[177,194,224],"\u22121":[178,195,225],"wet":[179,196,226],"weight,":[180],"Indonesian":[182],"river":[183],"sediment":[184,209],"had":[185],"only":[186,309],"4.6":[187],"2.7":[189],"2":[192],"weight.":[197,227],"In":[198],"addition":[199],"freshwater":[201],"environments,":[202],"sequences":[203,237,248,281,293],"also":[205],"detected":[206],"marine":[208],"North":[212],"Sea,":[213],"which":[214,306],"contained":[215],"approximately":[216],"2.5":[217],"0.7":[219],"4":[222],"Phylogenetic":[228],"analysis":[229],"revealed":[230],"amplified":[233],"from":[238,251],"environments":[241],"all":[242],"clustered":[243],"together":[244],"available":[246],"genome":[247],"known":[252],"archaea.":[257,287],"Taken":[258],"together,":[259],"these":[260,283],"demonstrate":[262],"validity":[264],"utility":[266],"quantitative":[273],"AOM":[286],"Furthermore,":[288],"newly":[290],"obtained":[291],"will":[294],"contribute":[295],"greater":[297],"resolution":[299],"sequences,":[305],"have":[307],"been":[308],"poorly":[310],"captured":[311],"by":[312],"general":[313],"methanogenic":[314],"primers.":[316]},"counts_by_year":[{"year":2026,"cited_by_count":15},{"year":2025,"cited_by_count":15},{"year":2024,"cited_by_count":16},{"year":2023,"cited_by_count":13},{"year":2022,"cited_by_count":16},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2017-01-26T00:00:00"}
