{"id":"https://openalex.org/W2569504572","doi":"https://doi.org/10.1016/j.jviromet.2016.12.018","title":"An efficient and high fidelity method for amplification, cloning and sequencing of complete tospovirus genomic RNA segments","display_name":"An efficient and high fidelity method for amplification, cloning and sequencing of complete tospovirus genomic RNA segments","publication_year":2017,"publication_date":"2017-01-09","ids":{"openalex":"https://openalex.org/W2569504572","doi":"https://doi.org/10.1016/j.jviromet.2016.12.018","mag":"2569504572","pmid":"https://pubmed.ncbi.nlm.nih.gov/28082165"},"language":"en","primary_location":{"id":"doi:10.1016/j.jviromet.2016.12.018","doi":"https://doi.org/10.1016/j.jviromet.2016.12.018","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jviromet.2016.12.018","pdf_url":"https://www.sciencedirect.com/science/article/am/pii/S0166093416304785","source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.sciencedirect.com/science/article/am/pii/S0166093416304785","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5022388438","display_name":"Spencer H. Marshall","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Spencer H. Marshall","raw_affiliation_strings":["Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States","institution_ids":["https://openalex.org/I72951846"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046297405","display_name":"Raphael O. Adegbola","orcid":"https://orcid.org/0000-0002-0608-2970","observed_orcids":["https://orcid.org/0000-0002-0608-2970"]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Raphael O. Adegbola","raw_affiliation_strings":["Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States","institution_ids":["https://openalex.org/I72951846"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027080394","display_name":"Scott T. Adkins","orcid":"https://orcid.org/0000-0003-0095-8414","observed_orcids":["https://orcid.org/0000-0003-0095-8414"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I1336096307","display_name":"United States Department of Agriculture","ror":"https://ror.org/01na82s61","country_code":"US","type":"government","lineage":["https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I4210155113","display_name":"U.S. Horticultural Research Laboratory","ror":"https://ror.org/04a4m2h70","country_code":"US","type":"facility","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210122704","https://openalex.org/I4210155113"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott Adkins","raw_affiliation_strings":["United States Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"United States Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, United States","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210155113"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5065677891","display_name":"R. A. Naidu","orcid":"https://orcid.org/0000-0002-7273-5116","observed_orcids":["https://orcid.org/0000-0002-7273-5116"]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Rayapati A. Naidu","raw_affiliation_strings":["Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States. Electronic address: naidu.rayapati@wsu.edu","Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States. Electronic address: naidu.rayapati@wsu.edu","institution_ids":["https://openalex.org/I72951846"]},{"raw_affiliation_string":"Washington State University, Department of Plant Pathology, Irrigated Agricultural Research and Extension Center, Prosser, WA 99350, United States","institution_ids":["https://openalex.org/I72951846"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5065677891"],"corresponding_institution_ids":["https://openalex.org/I72951846"],"apc_list":{"value":3210,"currency":"USD","value_usd":3210},"apc_paid":null,"fwci":0.7821,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.72116024,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"242","issue":null,"first_page":"22","last_page":"26"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13220","display_name":"Plant and Fungal Interactions Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12047","display_name":"Viral Infections and Vectors","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tospoviruses","display_name":"tospoviruses","score":0.9599999785423279},{"id":"https://openalex.org/keywords/tomato-spotted-wilt-virus","display_name":"tomato spotted wilt virus","score":0.9810000061988831},{"id":"https://openalex.org/keywords/impatiens-necrotic-spot-virus","display_name":"Impatiens necrotic spot virus","score":1.0},{"id":"https://openalex.org/keywords/m-segment","display_name":"M segment","score":0.621999979019165},{"id":"https://openalex.org/keywords/s-segment","display_name":"S segment","score":1.0},{"id":"https://openalex.org/keywords/sequencing","display_name":"sequencing","score":0.36899998784065247},{"id":"https://openalex.org/keywords/coinfection","display_name":"co-infection","score":0.9010000228881836},{"id":"https://openalex.org/keywords/bunyaviridae","display_name":"Bunyaviridae","score":0.8320000171661377},{"id":"https://openalex.org/keywords/plant-viruses","display_name":"plant viruses","score":0.34700000286102295}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9251998662948608},{"id":"https://openalex.org/C2779553184","wikidata":"https://www.wikidata.org/wiki/Q2716408","display_name":"Tospovirus","level":5,"score":0.7499002814292908},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.693730354309082},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6879208087921143},{"id":"https://openalex.org/C2779271554","wikidata":"https://www.wikidata.org/wiki/Q29000551","display_name":"Bunyaviridae","level":3,"score":0.5829330086708069},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.4679355323314667},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4670492708683014},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.41894856095314026},{"id":"https://openalex.org/C193252679","wikidata":"https://www.wikidata.org/wiki/Q242125","display_name":"Phylogenetic tree","level":3,"score":0.4174152612686157},{"id":"https://openalex.org/C20850961","wikidata":"https://www.wikidata.org/wiki/Q478216","display_name":"Virus classification","level":4,"score":0.4155368208885193},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4133131802082062},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4002174735069275},{"id":"https://openalex.org/C109110057","wikidata":"https://www.wikidata.org/wiki/Q1495434","display_name":"Plant virus","level":3,"score":0.31800100207328796},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.2978776693344116},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.24988725781440735},{"id":"https://openalex.org/C2910476756","wikidata":"https://www.wikidata.org/wiki/Q1706774","display_name":"Tomato spotted wilt virus","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D002043","descriptor_name":"Bunyaviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D016679","descriptor_name":"Genome, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017865","descriptor_name":"Tospovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017865","descriptor_name":"Tospovirus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D019251","descriptor_name":"Nucleocapsid","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jviromet.2016.12.018","doi":"https://doi.org/10.1016/j.jviromet.2016.12.018","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jviromet.2016.12.018","pdf_url":"https://www.sciencedirect.com/science/article/am/pii/S0166093416304785","source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},{"id":"pmid:28082165","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28082165","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virological methods","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.jviromet.2016.12.018","doi":"https://doi.org/10.1016/j.jviromet.2016.12.018","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jviromet.2016.12.018","pdf_url":"https://www.sciencedirect.com/science/article/am/pii/S0166093416304785","source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.40450000762939453,"display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1598916882","display_name":null,"funder_award_id":"WNPO 0616","funder_id":"https://openalex.org/F4320309918","funder_display_name":"College of Agricultural, Human and Natural Resource Sciences, Washington State University"},{"id":"https://openalex.org/G7263133586","display_name":null,"funder_award_id":"2012-68004-20166","funder_id":"https://openalex.org/F4320306114","funder_display_name":"U.S. Department of Agriculture"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320309918","display_name":"College of Agricultural, Human and Natural Resource Sciences, Washington State University","ror":"https://ror.org/05dk0ce17"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W611274908","https://openalex.org/W1267438978","https://openalex.org/W1924700818","https://openalex.org/W1965249716","https://openalex.org/W1975316819","https://openalex.org/W1992314272","https://openalex.org/W1994273846","https://openalex.org/W2010556403","https://openalex.org/W2024494685","https://openalex.org/W2040550645","https://openalex.org/W2050242597","https://openalex.org/W2058060507","https://openalex.org/W2058164434","https://openalex.org/W2059098055","https://openalex.org/W2062084853","https://openalex.org/W2070327435","https://openalex.org/W2078001317","https://openalex.org/W2095648928","https://openalex.org/W2106882534","https://openalex.org/W2110385116","https://openalex.org/W2111037262","https://openalex.org/W2128205699","https://openalex.org/W2151528904","https://openalex.org/W2152207030","https://openalex.org/W2159265399","https://openalex.org/W2513565899","https://openalex.org/W2595920264"],"related_works":["https://openalex.org/W2083536998","https://openalex.org/W2018235741","https://openalex.org/W2113353979","https://openalex.org/W1968141274","https://openalex.org/W2024494685","https://openalex.org/W7217917646","https://openalex.org/W1986526676","https://openalex.org/W2584135516","https://openalex.org/W2328343997","https://openalex.org/W1998837073"],"abstract_inverted_index":null,"counts_by_year":[{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2017-01-13T00:00:00"}