{"id":"https://openalex.org/W2567530740","doi":"https://doi.org/10.1158/1538-7445.am2015-3416","title":"Abstract 3416: IHC assessment of PBRM1 loss in colon and lung carcinomas","display_name":"Abstract 3416: IHC assessment of PBRM1 loss in colon and lung carcinomas","publication_year":2015,"publication_date":"2015-08-01","ids":{"openalex":"https://openalex.org/W2567530740","doi":"https://doi.org/10.1158/1538-7445.am2015-3416","mag":"2567530740"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2015-3416","doi":"https://doi.org/10.1158/1538-7445.am2015-3416","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2015-3416","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056250228","display_name":"Aihua Li","orcid":"https://orcid.org/0000-0003-0867-9823","observed_orcids":["https://orcid.org/0000-0003-0867-9823"]},"institutions":[{"id":"https://openalex.org/I1288289746","display_name":"Abcam (United States)","ror":"https://ror.org/02e1wjw63","country_code":"US","type":"company","lineage":["https://openalex.org/I1288289746","https://openalex.org/I4210151096"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aihua Li","raw_affiliation_strings":["1Epitomics, an Abcam company, Burlingame, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Epitomics, an Abcam company, Burlingame, CA;","institution_ids":["https://openalex.org/I1288289746"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Yongfu Gao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288289746","display_name":"Abcam (United States)","ror":"https://ror.org/02e1wjw63","country_code":"US","type":"company","lineage":["https://openalex.org/I1288289746","https://openalex.org/I4210151096"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yongfu Gao","raw_affiliation_strings":["1Epitomics, an Abcam company, Burlingame, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Epitomics, an Abcam company, Burlingame, CA;","institution_ids":["https://openalex.org/I1288289746"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040516986","display_name":"Yuekai Zhang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288289746","display_name":"Abcam (United States)","ror":"https://ror.org/02e1wjw63","country_code":"US","type":"company","lineage":["https://openalex.org/I1288289746","https://openalex.org/I4210151096"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yuekai Zhang","raw_affiliation_strings":["1Epitomics, an Abcam company, Burlingame, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Epitomics, an Abcam company, Burlingame, CA;","institution_ids":["https://openalex.org/I1288289746"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003117104","display_name":"Hongyang Pan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288289746","display_name":"Abcam (United States)","ror":"https://ror.org/02e1wjw63","country_code":"US","type":"company","lineage":["https://openalex.org/I1288289746","https://openalex.org/I4210151096"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hongyang Pan","raw_affiliation_strings":["1Epitomics, an Abcam company, Burlingame, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Epitomics, an Abcam company, Burlingame, CA;","institution_ids":["https://openalex.org/I1288289746"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111416531","display_name":"Jackie K. W. Chan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1288289746","display_name":"Abcam (United States)","ror":"https://ror.org/02e1wjw63","country_code":"US","type":"company","lineage":["https://openalex.org/I1288289746","https://openalex.org/I4210151096"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jackie K. Chan","raw_affiliation_strings":["1Epitomics, an Abcam company, Burlingame, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Epitomics, an Abcam company, Burlingame, CA;","institution_ids":["https://openalex.org/I1288289746"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080276829","display_name":"Ximing J. Yang","orcid":"https://orcid.org/0000-0002-3370-6208","observed_orcids":["https://orcid.org/0000-0002-3370-6208"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ximing J. Yang","raw_affiliation_strings":["2Department of Pathology, Northwestern University, Feinberg School of Medicine, Burlingame, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Department of Pathology, Northwestern University, Feinberg School of Medicine, Burlingame, CA","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030656435","display_name":"Taiying Chen","orcid":"https://orcid.org/0000-0001-9879-8919","observed_orcids":["https://orcid.org/0000-0001-9879-8919"]},"institutions":[{"id":"https://openalex.org/I1288289746","display_name":"Abcam (United States)","ror":"https://ror.org/02e1wjw63","country_code":"US","type":"company","lineage":["https://openalex.org/I1288289746","https://openalex.org/I4210151096"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Taiying Chen","raw_affiliation_strings":["1Epitomics, an Abcam company, Burlingame, CA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Epitomics, an Abcam company, Burlingame, CA;","institution_ids":["https://openalex.org/I1288289746"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"75","issue":"15_Supplement","first_page":"3416","last_page":"3416"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/baf180","display_name":"BAF180","score":0.8960000276565552},{"id":"https://openalex.org/keywords/protein-loss","display_name":"protein loss","score":0.49000000953674316},{"id":"https://openalex.org/keywords/colon-cancer","display_name":"colon cancer","score":0.9150000214576721},{"id":"https://openalex.org/keywords/lung-cancer","display_name":"lung cancer","score":1.0},{"id":"https://openalex.org/keywords/clear-cell-renal-cell-carcinoma","display_name":"clear cell renal cell carcinoma","score":0.9819999933242798},{"id":"https://openalex.org/keywords/chromatin-remodeling","display_name":"chromatin remodeling","score":0.7870000004768372},{"id":"https://openalex.org/keywords/rabbit-monoclonal-antibodies","display_name":"rabbit monoclonal antibodies","score":0.890999972820282},{"id":"https://openalex.org/keywords/antibody-characterization","display_name":"antibody characterization","score":0.6209999918937683},{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"immunohistochemistry","score":0.722000002861023},{"id":"https://openalex.org/keywords/tumor-progression-markers","display_name":"tumor progression markers","score":0.5120000243186951},{"id":"https://openalex.org/keywords/cardiomyocytes","display_name":"cardiomyocytes","score":0.5609999895095825},{"id":"https://openalex.org/keywords/melanocytic-neoplasms","display_name":"melanocytic tumors","score":0.7239999771118164}],"concepts":[{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.6877673864364624},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5752198696136475},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.5439732670783997},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4584050476551056},{"id":"https://openalex.org/C193270364","wikidata":"https://www.wikidata.org/wiki/Q17141925","display_name":"Tissue microarray","level":3,"score":0.44500988721847534},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.4272228479385376},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.39195629954338074},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38233309984207153},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14864754676818848},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1274237036705017},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2015-3416","doi":"https://doi.org/10.1158/1538-7445.am2015-3416","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2015-3416","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9789000153541565}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1979468709","https://openalex.org/W4286295784","https://openalex.org/W2017239168","https://openalex.org/W2979864342","https://openalex.org/W2948677855","https://openalex.org/W2004612544","https://openalex.org/W2480800106","https://openalex.org/W1608091617","https://openalex.org/W2119824662","https://openalex.org/W2907865819"],"abstract_inverted_index":{"Abstract":[0],"PBRM1":[1,29,58,70,93,109,135,169,217,273,303,326,334,354,386,414],"(also":[2],"known":[3],"as":[4,15,257,259],"BAF":[5],"180)":[6],"is":[7,32,316,345],"a":[8,95,117,317],"chromatin":[9],"remodeling":[10],"polybromodomain":[11],"protein":[12,30,90,170,355],"recently":[13],"identified":[14],"the":[16,89,222,253,301,383,425,430],"second":[17],"most":[18,33,312],"frequently":[19,34],"mutated":[20],"gene":[21],"in":[22,36,53,62,75,94,111,175,221,250,276,320,336,358,387,390,416],"renal":[23],"cell":[24,38,156,179,261,297,338,370],"carcinoma":[25],"(RCC).":[26],"Loss":[27],"of":[28,47,57,64,69,83,92,98,108,132,212,224,236,239,252,265,268,279,286,294,325,333,368,385,413,424,429],"expression":[31,91,327],"observed":[35,249],"clear":[37,260,296,337,369],"RCC":[39,50,339],"and":[40,55,100,113,148,162,177,192,197,255,291,311,360,376,418],"correlated":[41],"with":[42,49,134,323,347],"tumor":[43,101,198,233,269,313,388],"progression.":[44],"Approximately":[45],"40-50%":[46],"patients":[48],"have":[51],"mutations":[52],"PBRM1,":[54],"loss":[56,68,110,324,335,415],"was":[59,86,128,158,171,183,248,274,356],"also":[60],"reported":[61],"28%":[63],"melanocytic":[65],"neoplasms.":[66],"However,":[67],"has":[71],"not":[72],"been":[73],"studied":[74],"other":[76],"tumors":[77,240],"using":[78,116,160,190],"patient":[79],"tissues.":[80,102,330],"The":[81,126,181,331],"purpose":[82],"this":[84,343],"study":[85,351],"to":[87,381],"assess":[88],"wide-ranging":[96],"collection":[97],"normal":[99,196,213,226,309],"Here":[103],"we":[104],"report":[105],"our":[106],"findings":[107],"colon":[112,281,359,375,417],"lung":[114,254,289,361,377,419],"carcinomas":[115,251,282,378],"newly":[118],"developed":[119],"rabbit":[120],"monoclonal":[121],"antibody":[122,127,152,182,218],"(RabMAb),":[123],"Clone":[124],"EP342.":[125],"generated":[129],"by":[130,145,173,186,342],"immunization":[131],"rabbits":[133,142],"recombinant":[136],"protein.":[137],"Sera":[138],"collected":[139],"from":[140,153],"immunized":[141],"were":[143],"screened":[144],"ELISA,":[146],"WB":[147,174],"IHC.":[149],"After":[150],"fusion,":[151],"final":[154],"hybridoma":[155],"line":[157],"characterized":[159,185],"ELISA":[161],"Western":[163],"blot":[164],"(WB).":[165],"A":[166],"180":[167],"KDa":[168],"detected":[172,341],"A431":[176],"MCF7":[178],"lysates.":[180],"further":[184],"extensive":[187],"IHC":[188,202,411],"testing":[189],"formalin-fixed":[191],"paraffin-embedded":[193],"(FFPE)":[194],"human":[195,205],"tissue":[199,206],"microarray":[200,207],"(TMA).":[201],"analysis":[203,264],"on":[204,328,374],"(TMA)":[208],"comprising":[209,235],"seventeen":[210],"types":[211,238,267],"tissues":[214],"showed":[215,271,352],"that":[216,272,353,367],"stained":[219],"positively":[220],"nuclei":[223],"all":[225],"cells":[227,310],"except":[228],"cardiac":[229],"muscle":[230],"cells.":[231,314],"In":[232,299],"TMAs":[234,270],"fifteen":[237],"(2-4":[241],"cases":[242],"for":[243,433],"each":[244],"type),":[245],"negative":[246],"staining":[247],"colon,":[256],"well":[258],"RCC.":[262,298,371],"Further":[263,372],"specific":[266],"lost":[275,357],"5":[277],"out":[278,285,293],"30":[280],"(17%),":[283],"8":[284,292],"35":[287],"(14%)":[288],"carcinomas,":[290,362],"1844%)":[295],"summary,":[300],"RabMAb":[302],"antibody,":[304],"clone":[305,344],"EP342":[306],"specifically":[307],"labels":[308],"It":[315],"useful":[318],"tool":[319],"identifying":[321],"cancers":[322],"FFPE":[329],"rate":[332],"(44%)":[340],"consistent":[346],"literature":[348],"reported.":[349],"Our":[350],"but":[363],"less":[364],"frequent":[365],"than":[366],"studies":[373],"are":[379],"needed":[380],"clarify":[382],"role":[384],"progression":[389],"these":[391],"tumors.":[392],"Citation":[393],"Format:":[394],"Aihua":[395],"Li,":[396],"Yongfu":[397],"Gao,":[398],"Yuekai":[399],"Zhang,":[400],"Hongyang":[401],"Pan,":[402],"Jackie":[403],"K.":[404],"Chan,":[405],"Ximing":[406],"J.":[407],"Yang,":[408],"Taiying":[409],"Chen.":[410],"assessment":[412],"carcinomas.":[420],"[abstract].":[421],"In:":[422],"Proceedings":[423],"106th":[426],"Annual":[427],"Meeting":[428],"American":[431],"Association":[432],"Cancer":[434,444],"Research;":[435],"2015":[436],"Apr":[437],"18-22;":[438],"Philadelphia,":[439],"PA.":[440],"Philadelphia":[441],"(PA):":[442],"AACR;":[443],"Res":[445],"2015;75(15":[446],"Suppl):Abstract":[447],"nr":[448],"3416.":[449],"doi:10.1158/1538-7445.AM2015-3416":[450]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2017-01-06T00:00:00"}