{"id":"https://openalex.org/W2566065015","doi":"https://doi.org/10.1182/blood.v104.11.4136.4136","title":"Evidence That More Primitive Hematopoietic Progenitor Cells Are Concentrated within the CD133+ Cell Compartment of Cord Blood.","display_name":"Evidence That More Primitive Hematopoietic Progenitor Cells Are Concentrated within the CD133+ Cell Compartment of Cord Blood.","publication_year":2004,"publication_date":"2004-11-16","ids":{"openalex":"https://openalex.org/W2566065015","doi":"https://doi.org/10.1182/blood.v104.11.4136.4136","mag":"2566065015"},"language":"en","primary_location":{"id":"doi:10.1182/blood.v104.11.4136.4136","doi":"https://doi.org/10.1182/blood.v104.11.4136.4136","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v104.11.4136.4136","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110214987","display_name":"Simon N. Robinson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Simon Robinson","raw_affiliation_strings":["Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077666079","display_name":"Ting Niu","orcid":"https://orcid.org/0000-0003-1580-1014","observed_orcids":["https://orcid.org/0000-0003-1580-1014","https://orcid.org/0000-0002-8752-8734"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ting Niu","raw_affiliation_strings":["Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010366863","display_name":"Hong Yu Yang","orcid":"https://orcid.org/0000-0002-0174-8593","observed_orcids":["https://orcid.org/0000-0002-0174-8593"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hong Yang","raw_affiliation_strings":["Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029640288","display_name":"Jingjing Ng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jingjing Ng","raw_affiliation_strings":["Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025791815","display_name":"Sean L. O\u2019Connor","orcid":"https://orcid.org/0000-0002-6852-4463","observed_orcids":["https://orcid.org/0000-0002-6852-4463"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sean O\u2019Connor","raw_affiliation_strings":["Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112235396","display_name":"John D. McMannis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John McMannis","raw_affiliation_strings":["Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055885795","display_name":"Elizabeth J. Shpall","orcid":"https://orcid.org/0000-0003-3947-4126","observed_orcids":["https://orcid.org/0000-0003-3947-4126"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Elizabeth Shpall","raw_affiliation_strings":["Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Blood and Bone Marrow Transplantation, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA","institution_ids":["https://openalex.org/I1343551460"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1343551460"],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.33508944,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"104","issue":"11","first_page":"4136","last_page":"4136"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hematopoietic-progenitor-cells","display_name":"hematopoietic progenitor cells","score":0.9789999723434448},{"id":"https://openalex.org/keywords/umbilical-cord-blood","display_name":"umbilical cord blood","score":0.9259999990463257},{"id":"https://openalex.org/keywords/cd133","display_name":"CD133","score":0.9340000152587891},{"id":"https://openalex.org/keywords/cobblestone-area-forming-cells","display_name":"cobblestone area-forming cells","score":0.9229999780654907},{"id":"https://openalex.org/keywords/limiting-dilution","display_name":"limiting dilution","score":0.5230000019073486},{"id":"https://openalex.org/keywords/interleukin-3","display_name":"interleukin-3","score":0.6190000176429749},{"id":"https://openalex.org/keywords/granulocyte-colony-stimulating-factor","display_name":"granulocyte colony-stimulating factor","score":0.9890000224113464},{"id":"https://openalex.org/keywords/hematopoietic-stem-cell-transplantation","display_name":"hematopoietic stem cell transplantation","score":0.5590000152587891}],"concepts":[{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.7147690057754517},{"id":"https://openalex.org/C2780914630","wikidata":"https://www.wikidata.org/wiki/Q914952","display_name":"Cord blood","level":2,"score":0.6533649563789368},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.6240691542625427},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.5237640738487244},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.5189411640167236},{"id":"https://openalex.org/C184112429","wikidata":"https://www.wikidata.org/wiki/Q476174","display_name":"Serial dilution","level":3,"score":0.4981682300567627},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.47704213857650757},{"id":"https://openalex.org/C16930146","wikidata":"https://www.wikidata.org/wiki/Q4381253","display_name":"Stromal cell","level":2,"score":0.47560960054397583},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.4704058766365051},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4611305296421051},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.45342445373535156},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.38669493794441223},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3203047215938568},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.23183885216712952},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.22249814867973328},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1845950484275818},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07463353872299194},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.06748950481414795},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1182/blood.v104.11.4136.4136","doi":"https://doi.org/10.1182/blood.v104.11.4136.4136","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v104.11.4136.4136","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2946498882","https://openalex.org/W7172201294","https://openalex.org/W2378190061","https://openalex.org/W2042598375","https://openalex.org/W1973572922","https://openalex.org/W2019552920","https://openalex.org/W2080666748","https://openalex.org/W2162287418","https://openalex.org/W2463357218","https://openalex.org/W2064330839"],"abstract_inverted_index":{"Abstract":[0],"We":[1],"report":[2],"the":[3,6,16,44,84,132,170,188,213,217,303,305,310,336,354,367,375,396,401,421,437,459,478],"use":[4,85],"of":[5,22,32,43,86,101,125,216,232,270,284,290,298,307,325,333,352,356,364,382,444,461,480],"in":[7,94,119,193,224,273,302,309,335,366,374,391,395,405,411,420],"vitro":[8],"cobblestone":[9,61],"area-forming":[10],"cell":[11,19,50,67,138,210,296,488],"(CAFC)":[12],"assay":[13,38,190,426],"to":[14,60,105,166,431,449],"investigate":[15],"hematopoietic":[17],"progenitor":[18],"(HPC)":[20],"profile":[21],"pre-selection,":[23],"post-selection":[24,28],"positive":[25,175,287,337],"(CD133+)":[26,160],"and":[27,79,151,157,174,176,182,235,238,250,262,280,321],"negative":[29,177,291,368],"(CD133\u2212)":[30],"fractions":[31,178,292],"cord":[33],"blood":[34],"(CB).":[35],"The":[36,207,331,362,424],"CAFC":[37,57,156,181,189,308,334,357,365,383,388,407,425],"provides":[39],"a":[40,48,53,65,87,97,122,136,282,294,314,341],"qualitative":[41],"measure":[42],"HPC":[45,114,133,438,476,483],"contained":[46],"within":[47,131,135,187,436],"given":[49,54],"population":[51,139,454],"at":[52,76,222,265,316,343,384,458,477],"time-point.":[55,386],"Briefly,":[56],"give":[58],"rise":[59],"areas":[62],"(CA)":[63],"beneath":[64],"stromal":[66,214],"layer":[68],"that":[69,92,117,372,408,434,468],"can":[70,140],"be":[71,106,141],"visualized":[72],"by":[73,83,322,349],"light":[74],"microscopy":[75],"weekly":[77,266],"intervals":[78],"their":[80],"frequencies":[81,381],"determined":[82],"limiting":[88],"dilution":[89,186],"strategy.":[90],"CA":[91],"persist":[93,118,409],"culture":[95,120,326,353,412],"for":[96,121,155,180],"relatively":[98],"long":[99],"period":[100,124],"time":[102],"are":[103,417],"considered":[104],"derived":[107],"from":[108,278],"more":[109,474,481],"primitive,":[110],"potentially":[111,485],"higher":[112,414],"quality":[113,415],"than":[115,400],"those":[116],"shorter":[123],"time.":[126],"Using":[127],"this":[128],"approach,":[129],"changes":[130,433],"compartment":[134,439],"specific":[137],"quantified.":[142],"Methods:":[143],"Three":[144],"frozen":[145],"CB":[146,275,392,447],"units":[147],"were":[148,163,260],"thawed,":[149],"washed":[150],"pre-selection":[152,274,311,376],"samples":[153,276],"removed":[154],"flow":[158],"cytometric":[159],"analysis.":[161,184],"Samples":[162],"then":[164],"subjected":[165],"CD133+":[167,183,271,295,397,422,446],"selection":[168],"using":[169],"Miltenyi":[171],"MidiMACS":[172],"device":[173],"collected":[179],"Limiting":[185],"was":[191,220,327,358],"performed":[192,221],"flat-bottomed":[194],"96-well":[195],"plates,":[196],"over":[197],"12,":[198],"2.5-fold":[199],"serial":[200],"dilutions":[201],"with":[202,240,379,440],"10":[203,324,351],"wells":[204],"per":[205],"dilution.":[206],"murine":[208],"FBMD-1":[209],"line":[211],"provided":[212],"component":[215],"assay.":[218],"Culture":[219],"33\u00b0C":[223],"Iscove\u2019s":[225],"Modified":[226],"Dulbecco\u2019s":[227],"Medium":[228],"containing":[229],"10%":[230],"each":[231,385],"fetal":[233],"bovine":[234],"horse":[236],"serum":[237],"supplemented":[239],"10ng/ml":[241],"recombinant":[242,252],"human":[243,253],"interleukin":[244],"3":[245],"(R":[246],"&":[247],"D":[248],"Systems)":[249],"20ng/ml":[251],"granulocyte":[254],"colony-stimulating":[255],"factor":[256],"(Neupogen,":[257],"Amgen).":[258],"Cultures":[259],"assayed":[261],"medium":[263],"refreshed":[264],"intervals.":[267],"Results:":[268],"Purity":[269],"cells":[272,448],"ranged":[277],"1-3%":[279],"reached":[281,313,340],"maximum":[283,315,342],"98%":[285],"following":[286],"selection.":[288],"Analysis":[289],"revealed":[293],"purity":[297],"1\u20132%.":[299],"As":[300],"shown":[301],"figure,":[304],"frequency":[306,332,355],"fraction":[312,338,369,398,403],"week":[317,323,344,350],"5":[318,345],"(~400/106":[319],"cells)":[320,347],"reducing":[328],"(~40/106":[329],"cells).":[330],"also":[339,418],"(~6500/106":[346],"however,":[348],"still":[359],"~2000/106":[360],"cells.":[361],"pattern":[363],"closely":[370],"mirrored":[371],"observed":[373],"sample":[377],"although":[378],"reduced":[380],"Conclusion:":[387],"activity":[389],"present":[390],"is":[393,427,455],"concentrated":[394,419],"rather":[399],"CD133\u2212":[402],"and,":[404],"particular,":[406],"longer":[410],"(potentially":[413],"HPC)":[416],"fraction.":[423],"currently":[428],"being":[429],"used":[430],"track":[432],"occur":[435],"ex":[441,469],"vivo":[442,470],"expansion":[443,471],"isolated":[445],"determine":[450],"whether":[451],"any":[452,462],"one":[453],"preferentially":[456],"expanded":[457],"expense":[460,479],"other.":[463],"This":[464],"will":[465],"address":[466],"concerns":[467],"techniques":[472],"expand":[473],"mature":[475],"primitive":[482],"thereby":[484],"compromising":[486],"stem":[487],"reserve.":[489],"Figure":[490,491]},"counts_by_year":[],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2017-01-06T00:00:00"}