{"id":"https://openalex.org/W2565484204","doi":"https://doi.org/10.1101/pdb.prot093369","title":"Isolating DNA from Gram-Negative Bacteria","display_name":"Isolating DNA from Gram-Negative Bacteria","publication_year":2017,"publication_date":"2017-01-01","ids":{"openalex":"https://openalex.org/W2565484204","doi":"https://doi.org/10.1101/pdb.prot093369","mag":"2565484204","pmid":"https://pubmed.ncbi.nlm.nih.gov/28049781"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot093369","doi":"https://doi.org/10.1101/pdb.prot093369","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot093369","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060605798","display_name":"Michael R. Green","orcid":"https://orcid.org/0000-0003-3017-3298","observed_orcids":["https://orcid.org/0000-0003-3017-3298","https://orcid.org/0000-0001-6309-9472"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michael R. Green","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5112152140","display_name":"Joseph Sambrook","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph Sambrook","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2017","issue":"1","first_page":"pdb.prot093369","last_page":"pdb.prot093369"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6039999723434448,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6039999723434448,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.3244999945163727,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.025100000202655792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-extraction","display_name":"DNA extraction","score":0.7670000195503235},{"id":"https://openalex.org/keywords/gram-negative-bacteria","display_name":"Gram-negative bacteria","score":0.9559999704360962},{"id":"https://openalex.org/keywords/bacterial-lysis","display_name":"bacterial lysis","score":0.7760000228881836},{"id":"https://openalex.org/keywords/sodium-dodecyl-sulfate","display_name":"sodium dodecyl sulfate","score":0.9819999933242798},{"id":"https://openalex.org/keywords/proteinase-k","display_name":"proteinase K","score":0.9950000047683716},{"id":"https://openalex.org/keywords/dna-shearing","display_name":"DNA shearing","score":0.8960000276565552},{"id":"https://openalex.org/keywords/phenol-chloroform-extraction","display_name":"phenol-chloroform extraction","score":0.9359999895095825},{"id":"https://openalex.org/keywords/isopropanol-precipitation","display_name":"isopropanol precipitation","score":0.9390000104904175},{"id":"https://openalex.org/keywords/high-molecular-weight-dna","display_name":"high molecular weight DNA","score":0.9589999914169312}],"concepts":[{"id":"https://openalex.org/C20808630","wikidata":"https://www.wikidata.org/wiki/Q4727661","display_name":"Alkaline lysis","level":5,"score":0.7322394847869873},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.7317566871643066},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6840101480484009},{"id":"https://openalex.org/C2778471508","wikidata":"https://www.wikidata.org/wiki/Q425310","display_name":"Proteinase K","level":3,"score":0.682360053062439},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.6549608707427979},{"id":"https://openalex.org/C2781144210","wikidata":"https://www.wikidata.org/wiki/Q172275","display_name":"Chloroform","level":2,"score":0.6541218161582947},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.6309720873832703},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5873579978942871},{"id":"https://openalex.org/C2778913249","wikidata":"https://www.wikidata.org/wiki/Q422241","display_name":"Sodium dodecyl sulfate","level":2,"score":0.5249312520027161},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.494462251663208},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.46839284896850586},{"id":"https://openalex.org/C161369605","wikidata":"https://www.wikidata.org/wiki/Q41803","display_name":"Gram","level":3,"score":0.45738595724105835},{"id":"https://openalex.org/C2777702071","wikidata":"https://www.wikidata.org/wiki/Q130336","display_name":"Phenol","level":2,"score":0.45351260900497437},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37684887647628784},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34187576174736023},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.2981947064399719},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2706921696662903},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17016497254371643},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.12611240148544312},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.08176249265670776},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.08088570833206177},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07867532968521118},{"id":"https://openalex.org/C167508917","wikidata":"https://www.wikidata.org/wiki/Q578537","display_name":"DNA vaccination","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D001433","descriptor_name":"Bacteriolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002725","descriptor_name":"Chloroform","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003902","descriptor_name":"Detergents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006090","descriptor_name":"Gram-Negative Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006090","descriptor_name":"Gram-Negative Bacteria","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008967","descriptor_name":"Molecular Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D012967","descriptor_name":"Sodium Dodecyl Sulfate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012997","descriptor_name":"Solvents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019286","descriptor_name":"Endopeptidase K","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019800","descriptor_name":"Phenol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019840","descriptor_name":"2-Propanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot093369","doi":"https://doi.org/10.1101/pdb.prot093369","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot093369","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:28049781","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28049781","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4392542836","https://openalex.org/W4403983709","https://openalex.org/W2084706915","https://openalex.org/W4392594309","https://openalex.org/W1974532177","https://openalex.org/W3014759458","https://openalex.org/W1811554286","https://openalex.org/W4411669933","https://openalex.org/W3188275260","https://openalex.org/W1979588115"],"abstract_inverted_index":{"The":[0],"isolation":[1],"of":[2,14],"DNA":[3,26,46],"from":[4,53],"bacteria,":[5],"described":[6],"in":[7,58],"this":[8,50],"protocol,":[9],"relies":[10],"upon":[11],"the":[12,23],"use":[13],"sodium":[15],"dodecyl":[16],"sulfate":[17],"and":[18,34,42],"proteinase":[19],"K":[20],"to":[21,49,55],"lyse":[22],"cells.":[24],"High-molecular-weight":[25],"is":[27],"then":[28],"sheared":[29],"(to":[30],"reduce":[31],"its":[32],"viscosity":[33],"make":[35],"it":[36],"more":[37],"manageable),":[38],"extracted":[39],"with":[40,44],"phenol:chloroform,":[41],"precipitated":[43],"isopropanol.":[45],"isolated":[47],"according":[48],"procedure":[51],"ranges":[52],"30":[54],"80":[56],"kb":[57],"length.":[59]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":7},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-01-06T00:00:00"}
