{"id":"https://openalex.org/W2561950987","doi":"https://doi.org/10.1093/czoolo/61.5.835","title":"Comparing techniques for measuring corticosterone in tadpoles","display_name":"Comparing techniques for measuring corticosterone in tadpoles","publication_year":2015,"publication_date":"2015-10-01","ids":{"openalex":"https://openalex.org/W2561950987","doi":"https://doi.org/10.1093/czoolo/61.5.835","mag":"2561950987"},"language":"en","primary_location":{"id":"doi:10.1093/czoolo/61.5.835","doi":"https://doi.org/10.1093/czoolo/61.5.835","is_oa":true,"landing_page_url":"https://doi.org/10.1093/czoolo/61.5.835","pdf_url":null,"source":{"id":"https://openalex.org/S58136343","display_name":"Current Zoology","issn_l":"1674-5507","issn":["1674-5507","2058-5888","2396-9814"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","dongbi-d","jufo-1","norway-1","oarl","poland-100","poland-20","russia-white-list-1","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Zoology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.1093/czoolo/61.5.835","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049605846","display_name":"Pablo Burraco","orcid":"https://orcid.org/0000-0002-9007-2643","observed_orcids":["https://orcid.org/0000-0002-9007-2643"]},"institutions":[{"id":"https://openalex.org/I4210089289","display_name":"Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana","ror":"https://ror.org/006gw6z14","country_code":"ES","type":"facility","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210089289"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"Pablo Burraco","raw_affiliation_strings":["Department of Wetland Ecology, Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana (EBD-CSIC), C/ Am\u00e9rico Vespucio, s/n, 41092 Sevilla, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Wetland Ecology, Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana (EBD-CSIC), C/ Am\u00e9rico Vespucio, s/n, 41092 Sevilla, Spain","institution_ids":["https://openalex.org/I4210089289"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017179991","display_name":"Rosa Arribas","orcid":"https://orcid.org/0000-0002-2417-6049","observed_orcids":["https://orcid.org/0000-0002-2417-6049"]},"institutions":[{"id":"https://openalex.org/I4210089289","display_name":"Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana","ror":"https://ror.org/006gw6z14","country_code":"ES","type":"facility","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210089289"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Rosa Arribas","raw_affiliation_strings":["Department of Wetland Ecology, Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana (EBD-CSIC), C/ Am\u00e9rico Vespucio, s/n, 41092 Sevilla, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Wetland Ecology, Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana (EBD-CSIC), C/ Am\u00e9rico Vespucio, s/n, 41092 Sevilla, Spain","institution_ids":["https://openalex.org/I4210089289"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001139520","display_name":"Saurabh S. Kulkarni","orcid":"https://orcid.org/0000-0002-0882-6478","observed_orcids":["https://orcid.org/0000-0002-0882-6478"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Saurabh S. Kulkarni","raw_affiliation_strings":["Department of Pediatrics, Yale School of Medicine, Yale University, New Haven, CT 0651, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pediatrics, Yale School of Medicine, Yale University, New Haven, CT 0651, USA","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084792810","display_name":"Daniel R. Buchholz","orcid":"https://orcid.org/0000-0003-2070-5684","observed_orcids":["https://orcid.org/0000-0003-2070-5684"]},"institutions":[{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel R. Buchholz","raw_affiliation_strings":["Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA","institution_ids":["https://openalex.org/I63135867"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022599271","display_name":"Iv\u00e1n G\u00f3mez-Mestre","orcid":"https://orcid.org/0000-0003-0094-8195","observed_orcids":["https://orcid.org/0000-0003-0094-8195"]},"institutions":[{"id":"https://openalex.org/I4210089289","display_name":"Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana","ror":"https://ror.org/006gw6z14","country_code":"ES","type":"facility","lineage":["https://openalex.org/I134820265","https://openalex.org/I4210089289"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Ivan Gomez-Mestre","raw_affiliation_strings":["Department of Wetland Ecology, Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana (EBD-CSIC), C/ Am\u00e9rico Vespucio, s/n, 41092 Sevilla, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Wetland Ecology, Estaci\u00f3n Biol\u00f3gica de Do\u00f1ana (EBD-CSIC), C/ Am\u00e9rico Vespucio, s/n, 41092 Sevilla, Spain","institution_ids":["https://openalex.org/I4210089289"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5049605846"],"corresponding_institution_ids":["https://openalex.org/I4210089289"],"apc_list":{"value":0,"currency":"USD","value_usd":0},"apc_paid":null,"fwci":0.5209,"has_fulltext":false,"cited_by_count":24,"citation_normalized_percentile":{"value":0.65043125,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"61","issue":"5","first_page":"835","last_page":"845"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10529","display_name":"Stress Responses and Cortisol","score":0.8270999789237976,"subfield":{"id":"https://openalex.org/subfields/2802","display_name":"Behavioral Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10529","display_name":"Stress Responses and Cortisol","score":0.8270999789237976,"subfield":{"id":"https://openalex.org/subfields/2802","display_name":"Behavioral Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.051899999380111694,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12421","display_name":"Adrenal Hormones and Disorders","score":0.04179999977350235,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/corticosterone","display_name":"corticosterone","score":0.9929999709129333},{"id":"https://openalex.org/keywords/tadpoles","display_name":"tadpoles","score":0.7509999871253967},{"id":"https://openalex.org/keywords/xenopus-laevis","display_name":"Xenopus laevis","score":0.9959999918937683},{"id":"https://openalex.org/keywords/radioimmunoassay","display_name":"radioimmunoassay","score":0.906000018119812},{"id":"https://openalex.org/keywords/enzyme-immunoassay","display_name":"enzyme immunoassay","score":0.9990000128746033},{"id":"https://openalex.org/keywords/glucocorticoids","display_name":"glucocorticoids","score":0.7429999709129333},{"id":"https://openalex.org/keywords/stress-response","display_name":"stress response","score":0.75},{"id":"https://openalex.org/keywords/amphibian-endocrinology","display_name":"amphibian endocrinology","score":0.8299999833106995},{"id":"https://openalex.org/keywords/plasma-corticosterone","display_name":"plasma corticosterone","score":0.7369999885559082},{"id":"https://openalex.org/keywords/assay-sensitivity","display_name":"assay sensitivity","score":0.6449999809265137}],"concepts":[{"id":"https://openalex.org/C2780352252","wikidata":"https://www.wikidata.org/wiki/Q422543","display_name":"Corticosterone","level":3,"score":0.8334230184555054},{"id":"https://openalex.org/C176685330","wikidata":"https://www.wikidata.org/wiki/Q857638","display_name":"Radioimmunoassay","level":2,"score":0.7867265343666077},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.6439124345779419},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5990639925003052},{"id":"https://openalex.org/C2780420688","wikidata":"https://www.wikidata.org/wiki/Q759183","display_name":"Immunoassay","level":3,"score":0.5358458757400513},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.5187345147132874},{"id":"https://openalex.org/C2780841215","wikidata":"https://www.wikidata.org/wiki/Q422549","display_name":"Glucocorticoid","level":2,"score":0.5071983337402344},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4830351769924164},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.4496678113937378},{"id":"https://openalex.org/C2779535977","wikidata":"https://www.wikidata.org/wiki/Q1342298","display_name":"Xenopus","level":3,"score":0.4200279712677002},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12612470984458923},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1083267331123352},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0739743709564209},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1093/czoolo/61.5.835","doi":"https://doi.org/10.1093/czoolo/61.5.835","is_oa":true,"landing_page_url":"https://doi.org/10.1093/czoolo/61.5.835","pdf_url":null,"source":{"id":"https://openalex.org/S58136343","display_name":"Current Zoology","issn_l":"1674-5507","issn":["1674-5507","2058-5888","2396-9814"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","dongbi-d","jufo-1","norway-1","oarl","poland-100","poland-20","russia-white-list-1","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Zoology","raw_type":"journal-article"},{"id":"pmh:oai:digital.csic.es:10261/346053","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10261/346053","pdf_url":null,"source":{"id":"https://openalex.org/S4306400616","display_name":"DIGITAL.CSIC (Spanish National Research Council (CSIC))","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I134820265","host_organization_name":"Consejo Superior de Investigaciones Cient\u00edficas","host_organization_lineage":["https://openalex.org/I134820265"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"http://purl.org/coar/resource_type/c_6501"},{"id":"pmh:oai:open-archive.highwire.org:czoolo:61/5/835","doi":null,"is_oa":false,"landing_page_url":"http://cz.oxfordjournals.org/cgi/content/short/61/5/835","pdf_url":null,"source":{"id":"https://openalex.org/S4406923041","display_name":"HighWire Press Open Archive","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"TEXT"}],"best_oa_location":{"id":"doi:10.1093/czoolo/61.5.835","doi":"https://doi.org/10.1093/czoolo/61.5.835","is_oa":true,"landing_page_url":"https://doi.org/10.1093/czoolo/61.5.835","pdf_url":null,"source":{"id":"https://openalex.org/S58136343","display_name":"Current Zoology","issn_l":"1674-5507","issn":["1674-5507","2058-5888","2396-9814"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","dongbi-d","jufo-1","norway-1","oarl","poland-100","poland-20","russia-white-list-1","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Zoology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310256","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":82,"referenced_works":["https://openalex.org/W13573365","https://openalex.org/W571542637","https://openalex.org/W592520988","https://openalex.org/W1488901254","https://openalex.org/W1518787232","https://openalex.org/W1536241213","https://openalex.org/W1891571358","https://openalex.org/W1900169075","https://openalex.org/W1964782825","https://openalex.org/W1969934673","https://openalex.org/W1972304741","https://openalex.org/W1980369627","https://openalex.org/W1980515753","https://openalex.org/W1988157043","https://openalex.org/W1990262403","https://openalex.org/W1990623145","https://openalex.org/W1995589175","https://openalex.org/W2001403647","https://openalex.org/W2002508149","https://openalex.org/W2002682119","https://openalex.org/W2004766074","https://openalex.org/W2005638112","https://openalex.org/W2007138782","https://openalex.org/W2009959372","https://openalex.org/W2012169387","https://openalex.org/W2012350699","https://openalex.org/W2015790818","https://openalex.org/W2021993785","https://openalex.org/W2022606413","https://openalex.org/W2024078022","https://openalex.org/W2026787427","https://openalex.org/W2027145859","https://openalex.org/W2027474965","https://openalex.org/W2029004410","https://openalex.org/W2029342816","https://openalex.org/W2032905318","https://openalex.org/W2040244894","https://openalex.org/W2043662794","https://openalex.org/W2048586777","https://openalex.org/W2055796772","https://openalex.org/W2056640069","https://openalex.org/W2063737179","https://openalex.org/W2064315281","https://openalex.org/W2065838029","https://openalex.org/W2067612656","https://openalex.org/W2069073789","https://openalex.org/W2072070636","https://openalex.org/W2075238504","https://openalex.org/W2079126285","https://openalex.org/W2081114447","https://openalex.org/W2082356227","https://openalex.org/W2086950172","https://openalex.org/W2090498849","https://openalex.org/W2093559723","https://openalex.org/W2094481108","https://openalex.org/W2095953970","https://openalex.org/W2097075017","https://openalex.org/W2102000916","https://openalex.org/W2106579310","https://openalex.org/W2106733249","https://openalex.org/W2109198158","https://openalex.org/W2111760270","https://openalex.org/W2112173895","https://openalex.org/W2115078524","https://openalex.org/W2115499015","https://openalex.org/W2124222805","https://openalex.org/W2125274929","https://openalex.org/W2128822914","https://openalex.org/W2131602248","https://openalex.org/W2131927278","https://openalex.org/W2132539548","https://openalex.org/W2133928932","https://openalex.org/W2137048242","https://openalex.org/W2137945845","https://openalex.org/W2144331186","https://openalex.org/W2153649201","https://openalex.org/W2155414581","https://openalex.org/W2159823712","https://openalex.org/W2160391946","https://openalex.org/W2162111922","https://openalex.org/W2272063267","https://openalex.org/W2327438386"],"related_works":["https://openalex.org/W7096714483","https://openalex.org/W3186102564","https://openalex.org/W2801385314","https://openalex.org/W1875310899","https://openalex.org/W2950378410","https://openalex.org/W1990262403","https://openalex.org/W2002434090","https://openalex.org/W4406214974","https://openalex.org/W2169811566","https://openalex.org/W4404355827"],"abstract_inverted_index":{"Abstract":[0],"Glucocorticoids":[1],"play":[2],"a":[3,71,77,106,213],"key":[4],"role":[5],"in":[6,10,19,228,241,249],"mediating":[7],"stress":[8],"responses":[9],"vertebrates.":[11],"Corticosterone":[12],"(CORT)":[13],"is":[14,185,207,212],"the":[15,52,68,93,173,242],"main":[16],"glucocorticoid":[17],"produced":[18],"amphibians,":[20],"birds,":[21],"and":[22,24,42,115,132,140,143,149,162,169,181,204],"reptiles,":[23],"regulates":[25],"several":[26],"metabolic":[27],"functions.":[28],"The":[29],"most":[30],"common":[31],"methods":[32],"for":[33,70,80,100],"quantifying":[34,62],"CORT":[35,127,199,229,248],"are":[36,202,234],"competitive":[37],"binding":[38],"immunoassays:":[39],"radioimmunoassay":[40],"(RIA)":[41],"enzyme":[43],"immunoassay":[44],"(EIA).":[45],"RIA":[46,104,135,184],"has":[47],"been":[48],"broadly":[49],"used":[50,87,98],"since":[51],"1980\u2019s":[53],"but":[54],"it":[55],"requires":[56],"radioactivity.":[57],"Commercial":[58],"EIA":[59,112,116,144,155,209,217],"kits":[60],"permit":[61],"hormone":[63],"levels":[64,230],"without":[65],"radioactivity":[66],"although":[67],"requirement":[69],"larger":[72],"sample":[73,177],"volume":[74],"may":[75,221],"be":[76,196,222],"strong":[78],"limitation":[79],"measurements":[81],"involving":[82],"larval":[83],"amphibians.":[84,250],"Here":[85],"we":[86,237],"Xenopus":[88],"laevis":[89],"tadpoles":[90,124],"to":[91,224],"compare":[92],"performance":[94],"of":[95,102,109,119,175,244],"three":[96],"commonly":[97],"procedures":[99],"determination":[101],"CORT:":[103],"on":[105,113,117,145,210,218,247],"chloroform":[107],"extract":[108],"whole-body":[110,120,153,219],"homogenate,":[111],"plasma,":[114],"supernatant":[118],"homogenate.":[121],"We":[122],"treated":[123],"with":[125],"exogenous":[126],"at":[128],"0,":[129],"25,":[130],"50,":[131],"100":[133,163],"nM.":[134,164],"could":[136],"distinguish":[137],"between":[138,147,160],"0":[139,148,161],"25":[141],"nM,":[142,151],"plasma":[146,211],"50":[150],"whereas":[152],"homogenate":[154],"only":[156],"detected":[157],"significant":[158],"differences":[159,201,233],"Each":[165],"procedure":[166],"presents":[167],"advantages":[168],"disadvantages":[170],"regarding":[171],"sensitivity,":[172],"use":[174],"radioactivity,":[176],"size,":[178],"handling":[179],"time,":[180],"economic":[182],"cost.":[183],"preferred":[186],"when":[187,231],"studying":[188],"small-bodied":[189],"animals":[190],"from":[191],"which":[192],"blood":[193,205],"samples":[194],"cannot":[195],"obtained.":[197],"When":[198],"level":[200],"intermediate":[203],"sampling":[206],"possible,":[208],"good":[214],"non-radioactive":[215],"alternative.":[216],"homogenates":[220],"useful":[223],"assess":[225],"qualitative":[226],"changes":[227],"considerable":[232],"expected.":[235],"Finally,":[236],"discuss":[238],"our":[239],"findings":[240],"context":[243],"previous":[245],"studies":[246]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":4},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-17T09:40:24.721904","created_date":"2017-01-06T00:00:00"}