{"id":"https://openalex.org/W2560828720","doi":null,"title":"Pulsed Dilution Method for the Recovery of Aggregated Mouse TNF-\u03b1.","display_name":"Pulsed Dilution Method for the Recovery of Aggregated Mouse TNF-\u03b1.","publication_year":2017,"publication_date":"2017-04-01","ids":{"openalex":"https://openalex.org/W2560828720","mag":"2560828720","pmid":"https://pubmed.ncbi.nlm.nih.gov/28367471","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5346277"},"language":"en","primary_location":{"id":"pmid:28367471","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28367471","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Reports of biochemistry & molecular biology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5346277/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027310584","display_name":"Merat Mahmoodi","orcid":"https://orcid.org/0000-0001-5773-3051","observed_orcids":["https://orcid.org/0000-0001-5773-3051"]},"institutions":[{"id":"https://openalex.org/I165560825","display_name":"Mashhad University of Medical Sciences","ror":"https://ror.org/04sfka033","country_code":"IR","type":"education","lineage":["https://openalex.org/I165560825"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Merat Mahmoodi","raw_affiliation_strings":["Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran","institution_ids":["https://openalex.org/I165560825"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Maryam Ghodsi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165560825","display_name":"Mashhad University of Medical Sciences","ror":"https://ror.org/04sfka033","country_code":"IR","type":"education","lineage":["https://openalex.org/I165560825"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Maryam Ghodsi","raw_affiliation_strings":["Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran","institution_ids":["https://openalex.org/I165560825"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010867164","display_name":"Malihe Moghadam","orcid":"https://orcid.org/0000-0002-0286-529X","observed_orcids":["https://orcid.org/0000-0002-0286-529X"]},"institutions":[{"id":"https://openalex.org/I165560825","display_name":"Mashhad University of Medical Sciences","ror":"https://ror.org/04sfka033","country_code":"IR","type":"education","lineage":["https://openalex.org/I165560825"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Malihe Moghadam","raw_affiliation_strings":["Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immuno-Biochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran","institution_ids":["https://openalex.org/I165560825"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002259570","display_name":"Mojtaba Sankian","orcid":"https://orcid.org/0000-0003-0602-6431","observed_orcids":["https://orcid.org/0000-0003-0602-6431"]},"institutions":[{"id":"https://openalex.org/I165560825","display_name":"Mashhad University of Medical Sciences","ror":"https://ror.org/04sfka033","country_code":"IR","type":"education","lineage":["https://openalex.org/I165560825"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Mojtaba Sankian","raw_affiliation_strings":["Immuno-Biochemistry lab, Immunology Research Center, Buali Research Institute , School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immuno-Biochemistry lab, Immunology Research Center, Buali Research Institute , School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran","institution_ids":["https://openalex.org/I165560825"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I165560825"],"apc_list":null,"apc_paid":null,"fwci":0.0974,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.3997007,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"5","issue":"2","first_page":"103","last_page":"107"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tnf-alpha","display_name":"TNF-\u03b1","score":0.8209999799728394},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8560000061988831},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.800000011920929},{"id":"https://openalex.org/keywords/protein-refolding","display_name":"protein refolding","score":0.7919999957084656},{"id":"https://openalex.org/keywords/guanidine-hydrochloride","display_name":"guanidine hydrochloride","score":0.8199999928474426},{"id":"https://openalex.org/keywords/metal-affinity-chromatography","display_name":"metal affinity chromatography","score":0.8880000114440918},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.7649999856948853}],"concepts":[{"id":"https://openalex.org/C203750385","wikidata":"https://www.wikidata.org/wiki/Q1308970","display_name":"Inclusion bodies","level":4,"score":0.6954624652862549},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6505732536315918},{"id":"https://openalex.org/C96618451","wikidata":"https://www.wikidata.org/wiki/Q3028008","display_name":"Dilution","level":2,"score":0.6445640325546265},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6066031455993652},{"id":"https://openalex.org/C2779463914","wikidata":"https://www.wikidata.org/wiki/Q183309","display_name":"Guanidine","level":2,"score":0.6063801050186157},{"id":"https://openalex.org/C17991360","wikidata":"https://www.wikidata.org/wiki/Q21173843","display_name":"Tumor necrosis factor alpha","level":2,"score":0.5951263308525085},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.5389500260353088},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5245473980903625},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.4933129847049713},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.47913095355033875},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.46616417169570923},{"id":"https://openalex.org/C8985478","wikidata":"https://www.wikidata.org/wiki/Q903485","display_name":"Protein purification","level":2,"score":0.45490512251853943},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3879801630973816},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3294435739517212},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2719023823738098},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.07420521974563599},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmid:28367471","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28367471","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Reports of biochemistry & molecular biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:5346277","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5346277/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rep Biochem Mol Biol","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:1e7e52c721c440d6a43c4de7cabb72e1","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1e7e52c721c440d6a43c4de7cabb72e1","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Reports of Biochemistry and Molecular Biology, Vol 5, Iss 2, Pp 103-107 (2017)","raw_type":"article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:5346277","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5346277/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rep Biochem Mol Biol","raw_type":"Text"},"sustainable_development_goals":[{"score":0.428600013256073,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W1547744139","https://openalex.org/W1850804182","https://openalex.org/W1931534490","https://openalex.org/W1971071753","https://openalex.org/W1982457599","https://openalex.org/W2040856684","https://openalex.org/W2049322319","https://openalex.org/W2069974072","https://openalex.org/W2077294458","https://openalex.org/W2082592090","https://openalex.org/W2099626559","https://openalex.org/W2103751982","https://openalex.org/W2111760376","https://openalex.org/W2113196743","https://openalex.org/W2134551760","https://openalex.org/W2141212534","https://openalex.org/W2148633856","https://openalex.org/W2153728088","https://openalex.org/W2153753900","https://openalex.org/W2157013283"],"related_works":["https://openalex.org/W3005983903","https://openalex.org/W2136759479","https://openalex.org/W2001572521","https://openalex.org/W1998730258","https://openalex.org/W2053451744","https://openalex.org/W2381465721","https://openalex.org/W2065730670","https://openalex.org/W1991217106","https://openalex.org/W2349574078","https://openalex.org/W2387093567"],"abstract_inverted_index":{"BACKGROUND:":[0],"is":[1,19,26],"a":[2,27,39,81,96,138],"favorable":[3],"way":[4],"to":[5,43,47,59,101,136],"get":[6],"high":[7,139],"yield":[8,140],"of":[9,14,22,76,119,133,141],"protein;":[10],"however,":[11],"the":[12,20,45,68,77,102,120,131],"formation":[13],"cytoplasmic":[15],"inclusion":[16,61],"bodies,":[17],"which":[18],"consequence":[21],"insoluble":[23],"accumulated":[24],"proteins,":[25],"major":[28],"obstacle":[29],"in":[30],"this":[31,35,134],"system.":[32],"To":[33],"overcome":[34],"obstacle,":[36],"we":[37],"used":[38,58],"pulsed":[40,74],"dilution":[41,75],"method":[42,135],"convert":[44],"product":[46],"its":[48],"native":[49],"conformation.":[50],"METHODS:":[51],"Reducing":[52],"agent":[53],"and":[54],"guanidine":[55],"hydrochloride":[56],"were":[57],"solubilize":[60],"bodies":[62],"formed":[63],"after":[64],"TNF-(\u03b1)":[65,104,112,123],"expression.":[66],"Then,":[67],"refolding":[69,82],"procedure":[70],"was":[71,87,108,124],"performed":[72],"by":[73,89,110],"denatured":[78],"protein":[79,86,107],"into":[80],"buffer.":[83],"The":[84,106,116],"properly-folded":[85],"purified":[88,121],"metal":[90],"affinity":[91],"chromatography.":[92],"RESULTS:":[93],"SDS-PAGE":[94],"showed":[95],"19.9":[97],"kDa":[98],"band":[99],"related":[100],"mature":[103,143],"protein.":[105],"recognized":[109],"anti-mouse":[111],"on":[113],"western":[114],"blots.":[115],"final":[117],"concentration":[118],"recombinant":[122],"62.5":[125],"\u00b5g/mL.":[126],"CONCLUSIONS:":[127],"Our":[128],"study":[129],"demonstrates":[130],"efficiency":[132],"produce":[137],"folded":[142],"TNF-":[144],"(\u03b1).":[145]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2017,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-12-16T00:00:00"}
