{"id":"https://openalex.org/W2557730320","doi":"https://doi.org/10.1101/pdb.prot093377","title":"Precipitation of DNA with Ethanol","display_name":"Precipitation of DNA with Ethanol","publication_year":2016,"publication_date":"2016-12-01","ids":{"openalex":"https://openalex.org/W2557730320","doi":"https://doi.org/10.1101/pdb.prot093377","mag":"2557730320","pmid":"https://pubmed.ncbi.nlm.nih.gov/27934690"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot093377","doi":"https://doi.org/10.1101/pdb.prot093377","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot093377","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060605798","display_name":"Michael R. Green","orcid":"https://orcid.org/0000-0003-3017-3298","observed_orcids":["https://orcid.org/0000-0003-3017-3298","https://orcid.org/0000-0001-6309-9472"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michael R. Green","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5112152140","display_name":"Joseph Sambrook","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph Sambrook","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":148,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2016","issue":"12","first_page":"pdb.prot093377","last_page":"pdb.prot093377"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9775000214576721,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9775000214576721,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.01899999938905239,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12327","display_name":"Various Chemistry Research Topics","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-precipitation","display_name":"DNA precipitation","score":0.9810000061988831},{"id":"https://openalex.org/keywords/ethanol-precipitation","display_name":"ethanol precipitation","score":0.9869999885559082},{"id":"https://openalex.org/keywords/isopropanol","display_name":"isopropanol","score":0.8399999737739563},{"id":"https://openalex.org/keywords/desalination","display_name":"desalination","score":0.765999972820282},{"id":"https://openalex.org/keywords/microcentrifuge-tubes","display_name":"microcentrifuge tubes","score":0.8579999804496765},{"id":"https://openalex.org/keywords/dna-purification","display_name":"DNA purification","score":0.43700000643730164},{"id":"https://openalex.org/keywords/cations","display_name":"cations","score":0.48399999737739563}],"concepts":[{"id":"https://openalex.org/C53941386","wikidata":"https://www.wikidata.org/wiki/Q2571416","display_name":"Ethanol precipitation","level":3,"score":0.8578939437866211},{"id":"https://openalex.org/C2780161600","wikidata":"https://www.wikidata.org/wiki/Q153","display_name":"Ethanol","level":2,"score":0.8577144145965576},{"id":"https://openalex.org/C107054158","wikidata":"https://www.wikidata.org/wiki/Q25257","display_name":"Precipitation","level":2,"score":0.8410471081733704},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.7351273894309998},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6649887561798096},{"id":"https://openalex.org/C2777132085","wikidata":"https://www.wikidata.org/wiki/Q46220103","display_name":"Phosphate","level":2,"score":0.544823408126831},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.532592236995697},{"id":"https://openalex.org/C96616956","wikidata":"https://www.wikidata.org/wiki/Q285100","display_name":"Phosphate buffered saline","level":2,"score":0.5018360614776611},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22742107510566711},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.03921285271644592},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008967","descriptor_name":"Molecular Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D011232","descriptor_name":"Chemical Precipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012997","descriptor_name":"Solvents","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot093377","doi":"https://doi.org/10.1101/pdb.prot093377","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot093377","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:27934690","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27934690","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W1918119607","https://openalex.org/W1935036262","https://openalex.org/W2011287843","https://openalex.org/W2027305883","https://openalex.org/W2042143430","https://openalex.org/W2069056285","https://openalex.org/W2101519710","https://openalex.org/W2104729897","https://openalex.org/W2161971785","https://openalex.org/W2167435540","https://openalex.org/W3026348482"],"related_works":["https://openalex.org/W2093494937","https://openalex.org/W2072598949","https://openalex.org/W2042143430","https://openalex.org/W3048557293","https://openalex.org/W4235075532","https://openalex.org/W2739597542","https://openalex.org/W7117412698","https://openalex.org/W2400212424","https://openalex.org/W4206529436","https://openalex.org/W4402764917"],"abstract_inverted_index":{"DNA":[0,52,61],"can":[1,23],"be":[2,24],"precipitated":[3],"out":[4],"of":[5,10,33,50,60],"solution":[6],"for":[7,13],"the":[8,31,47,51],"removal":[9],"salts":[11],"and/or":[12],"resuspension":[14],"in":[15,62],"an":[16],"alternative":[17],"buffer.":[18],"Either":[19],"ethanol":[20,34,58],"or":[21],"isopropanol":[22],"used":[25],"to":[26,45],"achieve":[27],"this":[28],"purpose;":[29],"however,":[30],"use":[32],"is":[35],"generally":[36],"preferred.":[37],"Cations,":[38],"provided":[39],"as":[40],"salts,":[41],"are":[42],"typically":[43],"included":[44],"neutralize":[46],"negative":[48],"charge":[49],"phosphate":[53],"backbone.":[54],"This":[55],"method":[56],"describes":[57],"precipitation":[59],"microcentrifuge":[63],"tubes.":[64]},"counts_by_year":[{"year":2026,"cited_by_count":16},{"year":2025,"cited_by_count":11},{"year":2024,"cited_by_count":19},{"year":2023,"cited_by_count":24},{"year":2022,"cited_by_count":22},{"year":2021,"cited_by_count":22},{"year":2020,"cited_by_count":15},{"year":2019,"cited_by_count":11},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-12-08T00:00:00"}