{"id":"https://openalex.org/W2554416499","doi":null,"title":"Species identification of Collembola by means of PCR-based marker systems","display_name":"Species identification of Collembola by means of PCR-based marker systems","publication_year":2005,"publication_date":"2005-01-01","ids":{"openalex":"https://openalex.org/W2554416499","mag":"2554416499"},"language":"en","primary_location":{"id":"pmh:oai:elib.suub.uni-bremen.de:DISS/00010021","doi":null,"is_oa":false,"landing_page_url":"http://elib.suub.uni-bremen.de/peid=D00010021","pdf_url":null,"source":{"id":"https://openalex.org/S7407064325","display_name":"State and University Library Bremen","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://nbn-resolving.de/<identifier scheme=\"urn:nbn:de\">urn:nbn:de:gbv:46-diss000100215</identifier>","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006151336","display_name":"Ulrich Burkhardt","orcid":"https://orcid.org/0000-0003-0761-1632","observed_orcids":["https://orcid.org/0000-0003-0761-1632"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Burkhardt, Ulrich","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5006151336"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13950","display_name":"Collembola Taxonomy and Ecology Studies","score":0.9643999934196472,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13950","display_name":"Collembola Taxonomy and Ecology Studies","score":0.9643999934196472,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14399","display_name":"Invertebrate Taxonomy and Ecology","score":0.027400000020861626,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11494","display_name":"Coleoptera Taxonomy and Distribution","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/collembola","display_name":"Collembola","score":0.9959999918937683},{"id":"https://openalex.org/keywords/species-identification","display_name":"species identification","score":0.8759999871253967},{"id":"https://openalex.org/keywords/molecular-markers","display_name":"molecular markers","score":0.6359999775886536},{"id":"https://openalex.org/keywords/pcr-rflp","display_name":"PCR-RFLP","score":0.8190000057220459},{"id":"https://openalex.org/keywords/rapd","display_name":"RAPD","score":0.8059999942779541},{"id":"https://openalex.org/keywords/ap-pcr","display_name":"AP-PCR","score":0.890999972820282},{"id":"https://openalex.org/keywords/aflp","display_name":"AFLP","score":1.0},{"id":"https://openalex.org/keywords/its1","display_name":"ITS1","score":0.6200000047683716},{"id":"https://openalex.org/keywords/cox1","display_name":"mitochondrial COI","score":0.7369999885559082},{"id":"https://openalex.org/keywords/mitochondrial-coii","display_name":"mitochondrial COII","score":0.9589999914169312},{"id":"https://openalex.org/keywords/phylogeny","display_name":"phylogeny","score":0.7630000114440918},{"id":"https://openalex.org/keywords/species-delimitation","display_name":"species delimitation","score":0.6520000100135803},{"id":"https://openalex.org/keywords/morphological-key","display_name":"morphological identification key","score":0.35499998927116394}],"concepts":[{"id":"https://openalex.org/C168007829","wikidata":"https://www.wikidata.org/wiki/Q857299","display_name":"Restriction fragment length polymorphism","level":4,"score":0.8671875},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.8328219652175903},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7848550081253052},{"id":"https://openalex.org/C193252679","wikidata":"https://www.wikidata.org/wiki/Q242125","display_name":"Phylogenetic tree","level":3,"score":0.592158854007721},{"id":"https://openalex.org/C24586158","wikidata":"https://www.wikidata.org/wiki/Q27075","display_name":"Mitochondrial DNA","level":3,"score":0.5753214359283447},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5347843170166016},{"id":"https://openalex.org/C2777160741","wikidata":"https://www.wikidata.org/wiki/Q2311855","display_name":"Molecular marker","level":3,"score":0.5049377083778381},{"id":"https://openalex.org/C58301748","wikidata":"https://www.wikidata.org/wiki/Q1074969","display_name":"Restriction site","level":4,"score":0.4834638237953186},{"id":"https://openalex.org/C84289048","wikidata":"https://www.wikidata.org/wiki/Q7316314","display_name":"Restriction fragment","level":3,"score":0.4830508828163147},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.473967045545578},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4343443810939789},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1215008795261383}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:elib.suub.uni-bremen.de:DISS/00010021","doi":null,"is_oa":false,"landing_page_url":"http://elib.suub.uni-bremen.de/peid=D00010021","pdf_url":null,"source":{"id":"https://openalex.org/S7407064325","display_name":"State and University Library Bremen","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://nbn-resolving.de/<identifier scheme=\"urn:nbn:de\">urn:nbn:de:gbv:46-diss000100215</identifier>","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":277,"referenced_works":["https://openalex.org/W3135204","https://openalex.org/W5731969","https://openalex.org/W6842505","https://openalex.org/W27477686","https://openalex.org/W78853169","https://openalex.org/W140975100","https://openalex.org/W638252385","https://openalex.org/W926521905","https://openalex.org/W994590905","https://openalex.org/W1244776683","https://openalex.org/W1486325159","https://openalex.org/W1491713847","https://openalex.org/W1498015928","https://openalex.org/W1512997946","https://openalex.org/W1513423272","https://openalex.org/W1522639024","https://openalex.org/W1523336822","https://openalex.org/W1546639740","https://openalex.org/W1549001849","https://openalex.org/W1552842333","https://openalex.org/W1567370344","https://openalex.org/W1568370280","https://openalex.org/W1573068371","https://openalex.org/W1602528939","https://openalex.org/W1814296370","https://openalex.org/W1815772925","https://openalex.org/W1827672689","https://openalex.org/W1830245186","https://openalex.org/W1834060250","https://openalex.org/W1887166581","https://openalex.org/W1903050482","https://openalex.org/W1948727601","https://openalex.org/W1963688205","https://openalex.org/W1963953102","https://openalex.org/W1966240881","https://openalex.org/W1966426576","https://openalex.org/W1967106084","https://openalex.org/W1967273792","https://openalex.org/W1968142541","https://openalex.org/W1969963311","https://openalex.org/W1970835031","https://openalex.org/W1970957201","https://openalex.org/W1971199253","https://openalex.org/W1971370695","https://openalex.org/W1971641030","https://openalex.org/W1972432518","https://openalex.org/W1972906909","https://openalex.org/W1974670959","https://openalex.org/W1975579833","https://openalex.org/W1976087814","https://openalex.org/W1976125810","https://openalex.org/W1977934796","https://openalex.org/W1980638571","https://openalex.org/W1981045554","https://openalex.org/W1981429735","https://openalex.org/W1981799351","https://openalex.org/W1981811208","https://openalex.org/W1982714171","https://openalex.org/W1983314001","https://openalex.org/W1983925400","https://openalex.org/W1985695686","https://openalex.org/W1986724886","https://openalex.org/W1987360106","https://openalex.org/W1987452682","https://openalex.org/W1987972641","https://openalex.org/W1989005080","https://openalex.org/W1989322866","https://openalex.org/W1990247591","https://openalex.org/W1991937561","https://openalex.org/W1992689313","https://openalex.org/W1992690566","https://openalex.org/W1992807272","https://openalex.org/W1992842122","https://openalex.org/W1994030595","https://openalex.org/W1995604267","https://openalex.org/W1996211329","https://openalex.org/W1998596397","https://openalex.org/W1998646424","https://openalex.org/W2001873436","https://openalex.org/W2002171543","https://openalex.org/W2003682188","https://openalex.org/W2004139671","https://openalex.org/W2006677715","https://openalex.org/W2007111127","https://openalex.org/W2007270568","https://openalex.org/W2008362004","https://openalex.org/W2008945408","https://openalex.org/W2009015909","https://openalex.org/W2009157172","https://openalex.org/W2011451172","https://openalex.org/W2011590236","https://openalex.org/W2013005557","https://openalex.org/W2013367562","https://openalex.org/W2013696309","https://openalex.org/W2014558901","https://openalex.org/W2016186775","https://openalex.org/W2016706429","https://openalex.org/W2016814405","https://openalex.org/W2017849402","https://openalex.org/W2018302128","https://openalex.org/W2021048093","https://openalex.org/W2021616444","https://openalex.org/W2022144066","https://openalex.org/W2022867284","https://openalex.org/W2025412093","https://openalex.org/W2026001460","https://openalex.org/W2026503192","https://openalex.org/W2027518128","https://openalex.org/W2027813766","https://openalex.org/W2028340511","https://openalex.org/W2028466018","https://openalex.org/W2029460072","https://openalex.org/W2030483572","https://openalex.org/W2030711123","https://openalex.org/W2031155200","https://openalex.org/W2032738571","https://openalex.org/W2032950375","https://openalex.org/W2036751419","https://openalex.org/W2036880429","https://openalex.org/W2037351108","https://openalex.org/W2039011247","https://openalex.org/W2043173643","https://openalex.org/W2043916205","https://openalex.org/W2047437641","https://openalex.org/W2047479519","https://openalex.org/W2047608289","https://openalex.org/W2048441377","https://openalex.org/W2048552960","https://openalex.org/W2049359343","https://openalex.org/W2049887985","https://openalex.org/W2050133457","https://openalex.org/W2051801987","https://openalex.org/W2052374739","https://openalex.org/W2052414895","https://openalex.org/W2054874683","https://openalex.org/W2055428555","https://openalex.org/W2056353331","https://openalex.org/W2056798455","https://openalex.org/W2058473329","https://openalex.org/W2059171542","https://openalex.org/W2059241870","https://openalex.org/W2060892878","https://openalex.org/W2060956671","https://openalex.org/W2063743476","https://openalex.org/W2067640463","https://openalex.org/W2067697780","https://openalex.org/W2067927269","https://openalex.org/W2068365886","https://openalex.org/W2068687524","https://openalex.org/W2070833538","https://openalex.org/W2073380915","https://openalex.org/W2074991491","https://openalex.org/W2076728886","https://openalex.org/W2078360575","https://openalex.org/W2079307899","https://openalex.org/W2080504490","https://openalex.org/W2081271492","https://openalex.org/W2081858203","https://openalex.org/W2082928585","https://openalex.org/W2088446008","https://openalex.org/W2088587174","https://openalex.org/W2092512759","https://openalex.org/W2093467980","https://openalex.org/W2095013536","https://openalex.org/W2095021403","https://openalex.org/W2096822099","https://openalex.org/W2097346213","https://openalex.org/W2097382368","https://openalex.org/W2097498651","https://openalex.org/W2097829713","https://openalex.org/W2098282695","https://openalex.org/W2098771778","https://openalex.org/W2098775142","https://openalex.org/W2099109279","https://openalex.org/W2099514211","https://openalex.org/W2099620219","https://openalex.org/W2099901798","https://openalex.org/W2101172349","https://openalex.org/W2101365203","https://openalex.org/W2101738635","https://openalex.org/W2101970685","https://openalex.org/W2103192996","https://openalex.org/W2106780062","https://openalex.org/W2107095036","https://openalex.org/W2111517285","https://openalex.org/W2113677672","https://openalex.org/W2114703217","https://openalex.org/W2115432906","https://openalex.org/W2116074574","https://openalex.org/W2116116608","https://openalex.org/W2116738794","https://openalex.org/W2117134466","https://openalex.org/W2118260045","https://openalex.org/W2119033868","https://openalex.org/W2120644786","https://openalex.org/W2122023966","https://openalex.org/W2123505617","https://openalex.org/W2124820706","https://openalex.org/W2125262220","https://openalex.org/W2125887668","https://openalex.org/W2126364323","https://openalex.org/W2126718416","https://openalex.org/W2127063854","https://openalex.org/W2131680030","https://openalex.org/W2131901408","https://openalex.org/W2132204033","https://openalex.org/W2132264441","https://openalex.org/W2133902293","https://openalex.org/W2136837621","https://openalex.org/W2138213161","https://openalex.org/W2139476783","https://openalex.org/W2139506413","https://openalex.org/W2139678543","https://openalex.org/W2140643163","https://openalex.org/W2141718064","https://openalex.org/W2142269327","https://openalex.org/W2142431124","https://openalex.org/W2143843153","https://openalex.org/W2145608399","https://openalex.org/W2147280513","https://openalex.org/W2148108676","https://openalex.org/W2149575152","https://openalex.org/W2151170639","https://openalex.org/W2152468914","https://openalex.org/W2152475239","https://openalex.org/W2153133907","https://openalex.org/W2154793767","https://openalex.org/W2155570918","https://openalex.org/W2156434383","https://openalex.org/W2158126476","https://openalex.org/W2159266221","https://openalex.org/W2160388710","https://openalex.org/W2161444534","https://openalex.org/W2161863335","https://openalex.org/W2163133288","https://openalex.org/W2164460551","https://openalex.org/W2166295832","https://openalex.org/W2168911466","https://openalex.org/W2170487191","https://openalex.org/W2170864129","https://openalex.org/W2171114940","https://openalex.org/W2171319187","https://openalex.org/W2173288910","https://openalex.org/W2177602240","https://openalex.org/W2180857021","https://openalex.org/W2184362140","https://openalex.org/W2277807663","https://openalex.org/W2280149390","https://openalex.org/W2288632462","https://openalex.org/W2312773080","https://openalex.org/W2313668312","https://openalex.org/W2314657102","https://openalex.org/W2317036447","https://openalex.org/W2317990405","https://openalex.org/W2318254213","https://openalex.org/W2328028519","https://openalex.org/W2333910376","https://openalex.org/W2337809421","https://openalex.org/W2372399747","https://openalex.org/W2398755997","https://openalex.org/W2412125126","https://openalex.org/W2465316432","https://openalex.org/W2486703832","https://openalex.org/W2494321683","https://openalex.org/W2521004686","https://openalex.org/W2546414871","https://openalex.org/W2558217548","https://openalex.org/W2584187235","https://openalex.org/W2607628195","https://openalex.org/W2724173199","https://openalex.org/W2742429629","https://openalex.org/W2774639265","https://openalex.org/W2795394811","https://openalex.org/W2972684032","https://openalex.org/W2989345970","https://openalex.org/W3010157496","https://openalex.org/W3021964199"],"related_works":["https://openalex.org/W6980061129","https://openalex.org/W2039011247","https://openalex.org/W2054874683","https://openalex.org/W2789552284","https://openalex.org/W4283709718","https://openalex.org/W4382291841","https://openalex.org/W2965473793","https://openalex.org/W2078379917","https://openalex.org/W2928282410","https://openalex.org/W2744561708"],"abstract_inverted_index":{"The":[0,349],"MOLART":[1],"project":[2],"on":[3,139,161,425,511],"which":[4,25],"this":[5,80,208,278],"PhD":[6],"thesis":[7],"bases":[8],"deals":[9],"with":[10,31,151,184,528],"the":[11,52,66,73,103,108,162,165,169,254,275,289,317,326,336,353,426,450,465,503,667],"development":[12],"and":[13,20,34,62,72,87,91,107,154,196,241,268,284,302,400,412,420,495,536,544,549,576,614,649,675],"standardisation":[14],"of":[15,23,56,105,123,207,233,277,294,322,329,355,373,378,428,431,469,541],"molecular":[16,144,155,178,386,493,523,557],"methods":[17,414,515],"for":[18,100,148,245,282,407,467,532,538,545,555,618,654,689],"safe":[19],"efficient":[21,242],"identification":[22,140,156,243,551],"species":[24,49,64,375,381,447],"can":[26,131,567,682],"not":[27,343,362,416],"-":[28,36],"or":[29,471],"only":[30,451],"great":[32],"effort":[33,104],"expertise":[35],"be":[37,132,218,464,484,519,568,603,627,641,652,666,683],"distinguished":[38],"by":[39,46,141,212,474,492,644],"morphological":[40,142,152,185,202,213,530],"analysis.":[41],"These":[42],"aims":[43],"were":[44,89,146,158,189,227,259,311,342],"exemplified":[45],"two":[47,455],"problematic":[48],"groups":[50,448],"within":[51],"Collembola":[53],"as":[54,449],"examples":[55],"taxa":[57,491,622],"that":[58,129,180,236,288,453,566,581],"combine":[59],"ecological":[60],"importance":[61],"controversial":[63],"status,":[65],"Protaphorura":[67],"armata":[68,256,291,304],"group":[69,76,209,248,292,376],"(Onychiuridae,":[70],"Poduromorpha)":[71],"Isotoma":[74],"viridis":[75,167,247],"(Isotomidae,":[77],"Entomobryomorpha).":[78],"For":[79,164,253],"purpose,":[81],"DNA":[82,130,508,534,572],"sequencing,":[83],"PCR-RFLP,":[84],"RAPD,":[85],"AP-PCR":[86,413],"AFLP":[88,440,663],"applied":[90],"evaluated":[92],"according":[93],"to":[94,114,120,175,192,201,220,229,273,306,325,345,391,405,436,463,483,507,518,562,574,577,589,602,626,640,658,665,685],"their":[95,98,149,655],"diagnostic":[96],"power,":[97],"efficiency":[99],"different":[101,250,397],"taxa,":[102],"time":[106],"experience":[109],"required.":[110],"It":[111],"was":[112,173,481],"aimed":[113],"extract":[115],"information":[116,677],"from":[117,135,249,261,316,396,478,570],"individual":[118],"specimens":[119,393,543,565],"avoid":[121],"mixing":[122],"genetic":[124],"information.":[125],"We":[126],"could":[127,217],"show":[128],"extracted":[133],"successfully":[134],"single":[136,595],"specimens.":[137],"Based":[138,510],"keys,":[143],"markers":[145,286],"tested":[147,331,653],"accordance":[150,183],"taxonomy":[153],"tools":[157],"designed":[159],"based":[160],"results.":[163],"I.":[166,215,246],"group,":[168,257],"mitochondrial":[170,265,285,357,598,632],"COII":[171,267],"sequence":[172,351],"used":[174,228,520],"infer":[176],"a":[177,194,222,231,239,364,370,374,384,459,485,496,583,673,686],"phylogeny":[179],"is":[181,560],"in":[182,271,332,445,499,521,526,610,629,635],"findings.":[186],"Molecular":[187],"traits":[188],"found":[190,312,344,482,628],"suitable":[191,617,688],"provide":[193,238,363],"fast":[195,240],"more":[197,295,365,624],"robust":[198],"discrimination":[199],"compared":[200],"characters.":[203],"A":[204],"fourth":[205],"member":[206],"recently":[210],"proposed":[211,517],"hints,":[214],"caerulea,":[216],"confirmed":[219,352],"represent":[221,307,346],"distinct":[223,308,347],"species.":[224,298,309,334,348,432],"Genetic":[225],"differences":[226,616],"establish":[230],"set":[232],"restriction":[234],"enzymes":[235],"will":[237,382],"system":[244],"European":[251],"countries.":[252],"P.":[255,290,300,303],"sequences":[258],"obtained":[260],"three":[262,318],"gene":[263,319,358,596],"loci,":[264,320,597],"COI,":[266],"nuclear":[269,283,636],"ITS1,":[270],"order":[272],"resolve":[274],"systematics":[276],"group.":[279],"Concordant":[280],"patterns":[281,423,444],"suggest":[287],"consists":[293],"than":[296,430,586,634],"one":[297],"Especially":[299],"fimata":[301],"seem":[305,601],"Differences":[310],"between":[313],"phylogenies":[314],"derived":[315],"most":[321,604],"them":[323],"due":[324,506],"small":[327],"number":[328],"individuals":[330],"some":[333],"In":[335],"phylogenetic":[337],"trees,":[338],"morphologically":[339],"intermediate":[340],"forms":[341],"ITS1":[350],"findings":[354],"both":[356,446],"regions":[359],"but":[360],"did":[361,415],"detailed":[366,371],"taxonomic":[367],"resolution.":[368],"Obviously":[369],"resolution":[372],"consisting":[377],"about":[379,389],"40":[380],"require":[383],"larger":[385],"marker":[387,403],"set,":[388],"10":[390],"20":[392],"per":[394],"'species'":[395],"sampling":[398],"sites":[399],"several":[401],"independent":[402],"sets":[404],"check":[406],"possible":[408],"inconsistencies.":[409],"Both":[410],"RAPD":[411,648],"reveal":[417],"reproducible":[418,442,674],"results":[419],"yielded":[421,441],"fragment":[422,443],"rather":[424],"level":[427,687],"populations":[429],"Whereas":[433],"TE-AFLP":[434],"failed":[435],"produce":[437],"visible":[438],"bands,":[439],"method":[452],"includes":[454],"amplification":[456,470],"steps,":[457],"indicating":[458],"low":[460],"template":[461],"concentration":[462],"reason":[466],"failure":[468],"reproducibility.":[472],"Contamination":[473],"alien":[475,571],"DNA,":[476],"especially":[477,525],"gut":[479],"content,":[480],"severe":[486],"problem":[487],"when":[488],"investigating":[489],"small-sized":[490,542],"methods,":[494,662],"long-time":[497],"storage":[498],"ethanol":[500],"further":[501],"decreases":[502],"extraction":[504,575],"yield":[505,535],"degradation.":[509],"these":[512],"investigations,":[513],"standardised":[514],"are":[516,608,623],"future":[522,550,556],"taxonomy,":[524,531,558],"cooperation":[527],"traditional":[529],"optimising":[533],"purity":[537],"better":[539],"characterisation":[540],"establishing":[546],"reference":[547],"collections":[548],"keys.":[552],"As":[553],"implications":[554],"it":[559,671],"recommended":[561],"use":[563,578],"live":[564],"cleaned":[569],"prior":[573,657],"species-specific":[579],"primers":[580,588],"bear":[582],"lower":[584],"risk":[585],"universal":[587],"amplify":[590],"unwanted":[591],"contaminations.":[592],"When":[593],"sequencing":[594],"protein-coding":[599],"genes":[600,633],"appropriate":[605],"since":[606,670],"they":[607],"present":[609],"high":[611],"copy":[612],"numbers":[613],"fixed":[615],"discriminating":[619],"closely":[620],"related":[621],"likely":[625],"faster":[630,690],"evolving":[631],"sequences.":[637],"Methods":[638],"known":[639],"influenced":[642],"easily":[643],"reaction":[645],"conditions,":[646],"like":[647],"AP-PCR,":[650],"should":[651],"reproducibility":[656],"use.":[659],"Among":[660],"fingerprinting":[661],"seems":[664],"best":[668],"choice,":[669],"produces":[672],"complex":[676],"pattern":[678],"that,":[679],"if":[680],"desired,":[681],"reduced":[684],"taxon":[691],"identification.":[692]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-10-10T00:00:00"}
