{"id":"https://openalex.org/W2554098615","doi":"https://doi.org/10.1007/s13353-016-0374-1","title":"Evaluation of stability and validation of reference genes for RT-qPCR expression studies in rice plants under water deficit","display_name":"Evaluation of stability and validation of reference genes for RT-qPCR expression studies in rice plants under water deficit","publication_year":2016,"publication_date":"2016-11-22","ids":{"openalex":"https://openalex.org/W2554098615","doi":"https://doi.org/10.1007/s13353-016-0374-1","mag":"2554098615","pmid":"https://pubmed.ncbi.nlm.nih.gov/27878453"},"language":"en","primary_location":{"id":"doi:10.1007/s13353-016-0374-1","doi":"https://doi.org/10.1007/s13353-016-0374-1","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s13353-016-0374-1","pdf_url":null,"source":{"id":"https://openalex.org/S152710239","display_name":"Journal of Applied Genetics","issn_l":"1234-1983","issn":["1234-1983","2190-3883"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-140","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Genetics","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005379524","display_name":"Priscila Ariane Auler","orcid":"https://orcid.org/0000-0001-9045-7626","observed_orcids":["https://orcid.org/0000-0001-9045-7626"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":true,"raw_author_name":"Priscila Ariane Auler","raw_affiliation_strings":["Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil. pri_auler@hotmail.com","Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil. pri_auler@hotmail.com","institution_ids":["https://openalex.org/I169248161"]},{"raw_affiliation_string":"Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103129424","display_name":"Let\u00edcia Carvalho Benitez","orcid":"https://orcid.org/0000-0002-5900-1193","observed_orcids":["https://orcid.org/0000-0002-5900-1193"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Let\u00edcia Carvalho Benitez","raw_affiliation_strings":["Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047506772","display_name":"Marcelo Nogueira do Amaral","orcid":"https://orcid.org/0000-0003-3180-7803","observed_orcids":["https://orcid.org/0000-0003-3180-7803"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Marcelo Nogueira do Amaral","raw_affiliation_strings":["Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015326931","display_name":"Isabel Lopes Vighi","orcid":"https://orcid.org/0000-0002-9684-8422","observed_orcids":["https://orcid.org/0000-0002-9684-8422"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Isabel Lopes Vighi","raw_affiliation_strings":["Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040865497","display_name":"Gabriela dos Santos Rodrigues","orcid":"https://orcid.org/0009-0004-5106-399X","observed_orcids":["https://orcid.org/0009-0004-5106-399X"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Gabriela dos Santos Rodrigues","raw_affiliation_strings":["Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Luciano Carlos da Maia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Luciano Carlos da Maia","raw_affiliation_strings":["Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil","institution_ids":["https://openalex.org/I169248161"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051488499","display_name":"Eug\u00eania Jacira Bolacel Braga","orcid":"https://orcid.org/0000-0001-6364-7934","observed_orcids":["https://orcid.org/0000-0001-6364-7934"]},"institutions":[{"id":"https://openalex.org/I169248161","display_name":"Universidade Federal de Pelotas","ror":"https://ror.org/05msy9z54","country_code":"BR","type":"education","lineage":["https://openalex.org/I169248161"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Eugenia Jacira Bolacel Braga","raw_affiliation_strings":["Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil","institution_ids":["https://openalex.org/I169248161"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5005379524"],"corresponding_institution_ids":["https://openalex.org/I169248161"],"apc_list":{"value":3790,"currency":"USD","value_usd":3790},"apc_paid":null,"fwci":1.4379,"has_fulltext":false,"cited_by_count":44,"citation_normalized_percentile":{"value":0.81620512,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"58","issue":"2","first_page":"163","last_page":"177"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/reference-genes","display_name":"reference genes","score":0.9750000238418579},{"id":"https://openalex.org/keywords/gene-expression-normalization","display_name":"gene expression normalization","score":0.8700000047683716},{"id":"https://openalex.org/keywords/rice","display_name":"rice","score":0.6340000033378601},{"id":"https://openalex.org/keywords/drought-stress","display_name":"drought stress","score":0.9869999885559082},{"id":"https://openalex.org/keywords/rt-qpcr","display_name":"RT-qPCR","score":0.6299999952316284},{"id":"https://openalex.org/keywords/reference-gene-stability","display_name":"reference gene stability","score":0.7450000047683716},{"id":"https://openalex.org/keywords/soil-moisture","display_name":"soil moisture","score":0.574999988079071}],"concepts":[{"id":"https://openalex.org/C2779148672","wikidata":"https://www.wikidata.org/wiki/Q25326537","display_name":"Reference genes","level":4,"score":0.9861700534820557},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9121007919311523},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.7073841094970703},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.6128705143928528},{"id":"https://openalex.org/C134792803","wikidata":"https://www.wikidata.org/wiki/Q424886","display_name":"Glyceraldehyde 3-phosphate dehydrogenase","level":4,"score":0.47389310598373413},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.46994951367378235},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.45060962438583374},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.43182799220085144},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.32887858152389526},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3224617838859558}],"mesh":[{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012015","descriptor_name":"Reference Standards","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014867","descriptor_name":"Water","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D017343","descriptor_name":"Genes, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018506","descriptor_name":"Gene Expression Regulation, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018749","descriptor_name":"RNA, Plant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/s13353-016-0374-1","doi":"https://doi.org/10.1007/s13353-016-0374-1","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s13353-016-0374-1","pdf_url":null,"source":{"id":"https://openalex.org/S152710239","display_name":"Journal of Applied Genetics","issn_l":"1234-1983","issn":["1234-1983","2190-3883"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-140","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Genetics","raw_type":"journal-article"},{"id":"pmid:27878453","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27878453","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of applied genetics","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.5666999816894531,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321091","display_name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","ror":"https://ror.org/00x0ma614"},{"id":"https://openalex.org/F4320322025","display_name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","ror":"https://ror.org/03swz6y49"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W201242609","https://openalex.org/W234950505","https://openalex.org/W1534062799","https://openalex.org/W1577577364","https://openalex.org/W1965721868","https://openalex.org/W1976324993","https://openalex.org/W1986007439","https://openalex.org/W1988212891","https://openalex.org/W2003621878","https://openalex.org/W2038934471","https://openalex.org/W2064461433","https://openalex.org/W2075383833","https://openalex.org/W2086491289","https://openalex.org/W2092138026","https://openalex.org/W2099824652","https://openalex.org/W2105423509","https://openalex.org/W2107277218","https://openalex.org/W2107737163","https://openalex.org/W2110885661","https://openalex.org/W2111427334","https://openalex.org/W2112982595","https://openalex.org/W2114360920","https://openalex.org/W2125795583","https://openalex.org/W2126902408","https://openalex.org/W2128443399","https://openalex.org/W2133635336","https://openalex.org/W2139997876","https://openalex.org/W2146548897","https://openalex.org/W2146767320","https://openalex.org/W2150606529","https://openalex.org/W2154273881","https://openalex.org/W2159699532","https://openalex.org/W2162733993","https://openalex.org/W2332671895","https://openalex.org/W2435357660","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W3134364976","https://openalex.org/W2332671895","https://openalex.org/W2291853114","https://openalex.org/W2501477934","https://openalex.org/W2888625870","https://openalex.org/W3121478579","https://openalex.org/W2732144971","https://openalex.org/W2574367973","https://openalex.org/W1965735548","https://openalex.org/W4381611709"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-11-30T00:00:00"}