{"id":"https://openalex.org/W2552829790","doi":null,"title":"DETERMINATION GENETIC FACTORS CONTROLLING B-LACTEMASE ENZYME RELATED TO BLA TEM AND BLA SHV FAMILIES USING THE POLYMERASE CHAIN REACTION","display_name":"DETERMINATION GENETIC FACTORS CONTROLLING B-LACTEMASE ENZYME RELATED TO BLA TEM AND BLA SHV FAMILIES USING THE POLYMERASE CHAIN REACTION","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W2552829790","mag":"2552829790"},"language":"en","primary_location":{"id":"mag:2552829790","doi":null,"is_oa":false,"landing_page_url":"https://www.iasj.net/iasj/article/70535","pdf_url":null,"source":{"id":"https://openalex.org/S4306514417","display_name":"Iraqi Journal of Biotechnology","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Iraqi Journal of Biotechnology","raw_type":null},"parent_works":[],"lineage":["https://openalex.org/W2552829790"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026166355","display_name":"Sawsan Sajid Al-jubori","orcid":"https://orcid.org/0000-0002-6268-6058","observed_orcids":["https://orcid.org/0000-0002-6268-6058"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sawsan Sajid Al-jubori","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-6268-6058","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067326928","display_name":"Saad L. Hamed","orcid":"https://orcid.org/0000-0002-3207-6670","observed_orcids":["https://orcid.org/0000-0002-3207-6670"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Saad L. Hamed","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-3207-6670","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5079661767","display_name":"Marwah Hasan Al-Kaabi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Marwah Hasan Al-Kaabi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":95},"biblio":{"volume":"11","issue":"2","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-lactamase","display_name":"beta-lactamase","score":0.9010000228881836},{"id":"https://openalex.org/keywords/blatem","display_name":"blaTEM","score":0.6240000128746033},{"id":"https://openalex.org/keywords/blashv","display_name":"blaSHV","score":0.9980000257492065},{"id":"https://openalex.org/keywords/extended-spectrum-beta-lactamase","display_name":"extended-spectrum beta-lactamase","score":0.9679999947547913},{"id":"https://openalex.org/keywords/gram-negative-bacteria","display_name":"Gram-negative bacteria","score":0.7590000033378601},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.996999979019165},{"id":"https://openalex.org/keywords/proteus-mirabilis","display_name":"Proteus mirabilis","score":0.9980000257492065},{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":1.0},{"id":"https://openalex.org/keywords/plasmid-mediated-resistance","display_name":"plasmid-mediated resistance","score":0.8650000095367432},{"id":"https://openalex.org/keywords/chromosomal-beta-lactamase","display_name":"chromosomal beta-lactamase","score":0.8899999856948853},{"id":"https://openalex.org/keywords/antimicrobial-resistance","display_name":"antimicrobial resistance","score":0.6940000057220459}],"concepts":[{"id":"https://openalex.org/C2778039505","wikidata":"https://www.wikidata.org/wiki/Q311273","display_name":"Proteus mirabilis","level":4,"score":0.8241778612136841},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.7536777257919312},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.6803572177886963},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.6521492600440979},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6381678581237793},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5829413533210754},{"id":"https://openalex.org/C2777637488","wikidata":"https://www.wikidata.org/wiki/Q31856","display_name":"Pseudomonas aeruginosa","level":3,"score":0.5740200877189636},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5717161297798157},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.5149899125099182},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.49303171038627625},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4817407429218292},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34058284759521484},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.33512452244758606},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13884004950523376}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2552829790","doi":null,"is_oa":false,"landing_page_url":"https://www.iasj.net/iasj/article/70535","pdf_url":null,"source":{"id":"https://openalex.org/S4306514417","display_name":"Iraqi Journal of Biotechnology","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Iraqi Journal of Biotechnology","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W136515962","https://openalex.org/W602436075","https://openalex.org/W1766021674","https://openalex.org/W1963606386","https://openalex.org/W1976175476","https://openalex.org/W1984484048","https://openalex.org/W1989925309","https://openalex.org/W1989985563","https://openalex.org/W2015841341","https://openalex.org/W2033690627","https://openalex.org/W2049223946","https://openalex.org/W2074669461","https://openalex.org/W2097434661","https://openalex.org/W2098011668","https://openalex.org/W2101829109","https://openalex.org/W2105125809","https://openalex.org/W2114714153","https://openalex.org/W2115486016","https://openalex.org/W2116517411","https://openalex.org/W2119630062","https://openalex.org/W2151496242","https://openalex.org/W2168409658","https://openalex.org/W2334324963"],"related_works":["https://openalex.org/W2016498984","https://openalex.org/W163248615","https://openalex.org/W2939893615","https://openalex.org/W4250370147","https://openalex.org/W4395464831","https://openalex.org/W2378623914","https://openalex.org/W2371608728","https://openalex.org/W7011445772","https://openalex.org/W2729157549"],"abstract_inverted_index":{"Clinical":[0],"isolates":[1,21,52,70,79,85,112,120,200,230,242,266],"(75)":[2],"of":[3,72,92,114,192,202,212,240,255,268,276],"Gram-":[4],"negative":[5],"bacteria":[6],"were":[7,22,41],"isolated":[8],"from":[9,82],"patients":[10],"with":[11],"different":[12,25],"infections":[13],"at":[14],"Al-Kadhumia":[15],"Educational":[16],"hospital":[17],"in":[18,161,216,280],"Baghdad.":[19],"These":[20],"diagnosed":[23],"using":[24,59,174,193,221,256],"morphological":[26],"and":[27,36,48,64,90,126,147,151,181],"biochemical":[28],"test":[29,98],"followed":[30],"by":[31,157,173,186,274],"the":[32,60,65,107,138,158,163,175,182,187,210,222,253,257,281,290,299],"complementary":[33],"api":[34,37],"20E":[35],"20NE":[38],"test.":[39],"They":[40],"distributed":[42],"as":[43,118,169,178,225],"Escherichia":[44],"coli,":[45,123],"Proteus":[46],"mirabilis":[47,88,125],"Pseudomonas":[49],"aeruginosa":[50,128],"(25":[51],"for":[53,86,121],"each).":[54],"\u03b2-lactemase":[55],"production":[56],"was":[57,99,134,154,184,287,293],"investigated":[58],"rapid":[61],"iodometric":[62],"method":[63,160],"results":[66,254],"revealed":[67],"that":[68,110,198,238,261,298],"48":[69],"out":[71,113,201,267],"75(64%)":[73],"gave":[74,232],"a":[75,170,179,226,270],"positive":[76,116,271],"result":[77,108,117,272],".These":[78],"were,":[80],"21":[81],"E.coli":[83],"(84%),17":[84],"P.":[87,124],"(68%)":[89],"10":[91,229],"Ps.":[93,127],"aeruginosa(40%).The":[94],"double":[95],"disc":[96],"approximation":[97],"used":[100,294],"to":[101,136,144],"detect":[102,137],"Extended":[103],"spectrum":[104],"\u03b2-lactemases":[105,142,239],"(ESBLs)and":[106],"showed":[109,197,260],"28":[111],"48(58.33%)gave":[115],"19,3,6":[119],"E.":[122,264],"respectively.":[129],"Polymerase":[130],"chain":[131],"reaction(PCR)":[132],"technique":[133],"performed":[135],"genetic":[139],"factors":[140],"controlling":[141],"related":[143],"bla":[145,148,205,249],"TEM":[146,206],"SHV":[149,250],"families":[150],"template":[152,171,180,227,259,292],"DNA":[153,166,177,196,224],"prepared":[155,185],"either":[156],"boiling":[159],"which":[162,235,295],"total":[164,194],"genomic":[165,195],"will":[167],"serve":[168],"or":[172],"plasmid":[176,223,246,291],"later":[183],"alkaline":[188],"lysis":[189],"method.":[190],"Results":[191],"27":[199],"28(96.42)were":[203],"harboring":[204],"gene":[207,251],"based":[208],"on":[209],"presence":[211,275],"950":[213],"bp":[214,278],"bands":[215],"1%":[217],"agarose":[218,282],"gel":[219,283],".By":[220],",only":[228],"(35.7%)":[231],"clear":[233],"band":[234,279,286],"may":[236,243,296],"indicate":[237,297],"these":[241],"be":[244],"are":[245],"mediated":[247],".For":[248],",":[252],"first":[258],"only":[262],"2":[263],"coli":[265],"28(4.14%)gave":[269],"represented":[273],"800":[277],"while":[284],"no":[285],"observed":[288],"when":[289],"enzymes":[300],"here":[301],"under":[302],"chromosomal":[303],"control.":[304]},"counts_by_year":[{"year":2020,"cited_by_count":2}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-11-30T00:00:00"}
