{"id":"https://openalex.org/W2552060428","doi":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","title":"Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays","display_name":"Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays","publication_year":2016,"publication_date":"2016-11-15","ids":{"openalex":"https://openalex.org/W2552060428","doi":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","mag":"2552060428","pmid":"https://pubmed.ncbi.nlm.nih.gov/27863990"},"language":"en","primary_location":{"id":"doi:10.1016/j.freeradbiomed.2016.11.019","doi":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","pdf_url":"https://www.sciencedirect.com/science/article/pii/S089158491631036X/pdf","source":{"id":"https://openalex.org/S128018148","display_name":"Free Radical Biology and Medicine","issn_l":"0891-5849","issn":["0891-5849","1873-4596"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Free Radical Biology and Medicine","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://www.sciencedirect.com/science/article/pii/S089158491631036X/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040888217","display_name":"Fl\u00e1via Rezende","orcid":"https://orcid.org/0000-0001-7864-9131","observed_orcids":["https://orcid.org/0000-0001-7864-9131"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Fl\u00e1via Rezende","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027165633","display_name":"Kim-Kristin Prior","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Kim-Kristin Prior","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002327697","display_name":"Oliver L\u00f6we","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Oliver L\u00f6we","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008201869","display_name":"Ilka Wittig","orcid":"https://orcid.org/0000-0002-9751-8054","observed_orcids":["https://orcid.org/0000-0002-9751-8054"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ilka Wittig","raw_affiliation_strings":["Functional Proteomics, SFB 815 Core Unit, Goethe-Universit\u00e4t, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Functional Proteomics, SFB 815 Core Unit, Goethe-Universit\u00e4t, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030324297","display_name":"Valentina Strecker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Valentina Strecker","raw_affiliation_strings":["Functional Proteomics, SFB 815 Core Unit, Goethe-Universit\u00e4t, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Functional Proteomics, SFB 815 Core Unit, Goethe-Universit\u00e4t, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043399603","display_name":"Franziska Moll","orcid":"https://orcid.org/0000-0002-6694-4608","observed_orcids":["https://orcid.org/0000-0002-6694-4608"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Franziska Moll","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083473276","display_name":"Valeska Helfinger","orcid":"https://orcid.org/0000-0002-7103-2144","observed_orcids":["https://orcid.org/0000-0002-7103-2144"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Valeska Helfinger","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008077143","display_name":"Frank Schn\u00fctgen","orcid":"https://orcid.org/0000-0002-2426-6685","observed_orcids":["https://orcid.org/0000-0002-2426-6685"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Frank Schn\u00fctgen","raw_affiliation_strings":["Institute for Molecular Hematology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Hematology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059314475","display_name":"Nina Kurrle","orcid":"https://orcid.org/0000-0003-0290-1489","observed_orcids":["https://orcid.org/0000-0003-0290-1489"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Nina Kurrle","raw_affiliation_strings":["Institute for Molecular Hematology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Hematology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076963906","display_name":"Frank Wempe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Frank Wempe","raw_affiliation_strings":["Institute for Molecular Hematology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Hematology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030474855","display_name":"Maria Walter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Maria Walter","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009921503","display_name":"Sven Zukunft","orcid":"https://orcid.org/0000-0002-1811-4562","observed_orcids":["https://orcid.org/0000-0002-1811-4562"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sven Zukunft","raw_affiliation_strings":["Institute for Vascular Signaling, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Vascular Signaling, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050567539","display_name":"Bert Luck","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Bert Luck","raw_affiliation_strings":["Institute for Vascular Signaling, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Vascular Signaling, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091173895","display_name":"Ingrid Fleming","orcid":"https://orcid.org/0000-0003-1881-3635","observed_orcids":["https://orcid.org/0000-0003-1881-3635"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ingrid Fleming","raw_affiliation_strings":["Institute for Vascular Signaling, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Vascular Signaling, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057524424","display_name":"Norbert Wei\u00dfmann","orcid":"https://orcid.org/0000-0003-2675-3871","observed_orcids":["https://orcid.org/0000-0003-2675-3871"]},"institutions":[{"id":"https://openalex.org/I200763008","display_name":"Justus-Liebig-Universit\u00e4t Gie\u00dfen","ror":"https://ror.org/033eqas34","country_code":"DE","type":"education","lineage":["https://openalex.org/I200763008"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Norbert Weissmann","raw_affiliation_strings":["University of Giessen, Lung Center, Giessen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Giessen, Lung Center, Giessen, Germany","institution_ids":["https://openalex.org/I200763008"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082251197","display_name":"Ralf Peter Brandes","orcid":"https://orcid.org/0000-0002-8035-0048","observed_orcids":["https://orcid.org/0000-0002-8035-0048"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Ralf P. Brandes","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany. Electronic address: brandes@vrc.uni-franfurt.de","Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany. Electronic address: brandes@vrc.uni-franfurt.de","institution_ids":["https://openalex.org/I114090438"]},{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013415164","display_name":"Katrin Schr\u00f6der","orcid":"https://orcid.org/0000-0002-3099-526X","observed_orcids":["https://orcid.org/0000-0002-3099-526X"]},"institutions":[{"id":"https://openalex.org/I114090438","display_name":"Goethe University Frankfurt","ror":"https://ror.org/04cvxnb49","country_code":"DE","type":"education","lineage":["https://openalex.org/I114090438"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Katrin Schr\u00f6der","raw_affiliation_strings":["Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany. Electronic address: schroeder@vrc.uni-frankfurt.de","Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany. Electronic address: schroeder@vrc.uni-frankfurt.de","institution_ids":["https://openalex.org/I114090438"]},{"raw_affiliation_string":"Institute for Cardiovascular Physiology, Goethe-University, Frankfurt, Germany","institution_ids":["https://openalex.org/I114090438"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5013415164","https://openalex.org/A5082251197"],"corresponding_institution_ids":["https://openalex.org/I114090438"],"apc_list":{"value":4240,"currency":"USD","value_usd":4240},"apc_paid":{"value":4240,"currency":"USD","value_usd":4240},"fwci":2.6143,"has_fulltext":true,"cited_by_count":46,"citation_normalized_percentile":{"value":0.90831861,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":94,"max":99},"biblio":{"volume":"102","issue":null,"first_page":"57","last_page":"66"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.6425999999046326,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.6425999999046326,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11597","display_name":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","score":0.18080000579357147,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.050599999725818634,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nadph-oxidase","display_name":"NADPH oxidase","score":0.8230000138282776},{"id":"https://openalex.org/keywords/cytochrome-p450","display_name":"cytochrome P450","score":0.9229999780654907},{"id":"https://openalex.org/keywords/lucigenin","display_name":"lucigenin","score":0.9449999928474426},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.9269999861717224},{"id":"https://openalex.org/keywords/nadph-cytochrome-p450-reductase","display_name":"NADPH-cytochrome P450 reductase","score":0.9010000228881836},{"id":"https://openalex.org/keywords/cytochrome-b5","display_name":"cytochrome b5","score":0.7179999947547913},{"id":"https://openalex.org/keywords/nox4","display_name":"NOX4","score":0.6570000052452087},{"id":"https://openalex.org/keywords/nox5","display_name":"NADPH oxidase 5","score":0.9620000123977661},{"id":"https://openalex.org/keywords/p22phox","display_name":"p22phox","score":0.824999988079071},{"id":"https://openalex.org/keywords/angiotensin-ii","display_name":"angiotensin II","score":0.8130000233650208},{"id":"https://openalex.org/keywords/smooth-muscle-cells","display_name":"smooth muscle cells","score":0.890999972820282},{"id":"https://openalex.org/keywords/crispr-cas9-knockout","display_name":"CRISPR/Cas9 knockout","score":0.7870000004768372}],"concepts":[{"id":"https://openalex.org/C2779719074","wikidata":"https://www.wikidata.org/wiki/Q2038057","display_name":"NADPH oxidase","level":3,"score":0.8585734367370605},{"id":"https://openalex.org/C2776061955","wikidata":"https://www.wikidata.org/wiki/Q18038565","display_name":"NOX1","level":4,"score":0.7143173217773438},{"id":"https://openalex.org/C2779765511","wikidata":"https://www.wikidata.org/wiki/Q18039812","display_name":"NOX4","level":4,"score":0.7133656740188599},{"id":"https://openalex.org/C2779127771","wikidata":"https://www.wikidata.org/wiki/Q3838769","display_name":"Lucigenin","level":4,"score":0.5635036826133728},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5490758419036865},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5091258883476257},{"id":"https://openalex.org/C48349386","wikidata":"https://www.wikidata.org/wiki/Q424361","display_name":"Reactive oxygen species","level":2,"score":0.5085054636001587},{"id":"https://openalex.org/C2780795997","wikidata":"https://www.wikidata.org/wiki/Q413283","display_name":"Superoxide","level":3,"score":0.5057156085968018},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.37872475385665894},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3347359895706177},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3082655072212219}],"mesh":[{"descriptor_ui":"D000074624","descriptor_name":"NADPH Oxidase 1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000074662","descriptor_name":"NADPH Oxidase 2","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000074663","descriptor_name":"NADPH Oxidase 4","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000166","descriptor_name":"Acridines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000166","descriptor_name":"Acridines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000804","descriptor_name":"Angiotensin II","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003573","descriptor_name":"Cytochrome b Group","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003577","descriptor_name":"Cytochrome P-450 Enzyme System","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003577","descriptor_name":"Cytochrome P-450 Enzyme System","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008566","descriptor_name":"Membranes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008566","descriptor_name":"Membranes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009251","descriptor_name":"NADPH-Ferrihemoprotein Reductase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017382","descriptor_name":"Reactive Oxygen Species","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018345","descriptor_name":"Mice, Knockout","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019255","descriptor_name":"NADPH Oxidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D019255","descriptor_name":"NADPH Oxidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D032389","descriptor_name":"Myocytes, Smooth Muscle","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049449","descriptor_name":"Luminescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.freeradbiomed.2016.11.019","doi":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","pdf_url":"https://www.sciencedirect.com/science/article/pii/S089158491631036X/pdf","source":{"id":"https://openalex.org/S128018148","display_name":"Free Radical Biology and Medicine","issn_l":"0891-5849","issn":["0891-5849","1873-4596"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Free Radical Biology and Medicine","raw_type":"journal-article"},{"id":"pmid:27863990","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27863990","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Free radical biology & medicine","raw_type":"Journal Article"},{"id":"pmh:oai:publikationen.ub.uni-frankfurt.de:45567","doi":null,"is_oa":true,"landing_page_url":"http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45567","pdf_url":null,"source":{"id":"https://openalex.org/S4306400432","display_name":"Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114090438","host_organization_name":"Goethe University Frankfurt","host_organization_lineage":["https://openalex.org/I114090438"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.freeradbiomed.2016.11.019","doi":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.freeradbiomed.2016.11.019","pdf_url":"https://www.sciencedirect.com/science/article/pii/S089158491631036X/pdf","source":{"id":"https://openalex.org/S128018148","display_name":"Free Radical Biology and Medicine","issn_l":"0891-5849","issn":["0891-5849","1873-4596"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Free Radical Biology and Medicine","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2552060428.pdf","grobid_xml":"https://content.openalex.org/works/W2552060428.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W1510406077","https://openalex.org/W1619653867","https://openalex.org/W1765775539","https://openalex.org/W1895145214","https://openalex.org/W1966866216","https://openalex.org/W1971202292","https://openalex.org/W1977444929","https://openalex.org/W1997100970","https://openalex.org/W2014744644","https://openalex.org/W2016667239","https://openalex.org/W2019411611","https://openalex.org/W2022368219","https://openalex.org/W2023464535","https://openalex.org/W2051639214","https://openalex.org/W2052673116","https://openalex.org/W2068403201","https://openalex.org/W2072238534","https://openalex.org/W2080752012","https://openalex.org/W2083796374","https://openalex.org/W2085128107","https://openalex.org/W2089796673","https://openalex.org/W2096094438","https://openalex.org/W2096869450","https://openalex.org/W2110182057","https://openalex.org/W2114355849","https://openalex.org/W2115528784","https://openalex.org/W2118736308","https://openalex.org/W2118943937","https://openalex.org/W2121438567","https://openalex.org/W2142841050","https://openalex.org/W2145681094","https://openalex.org/W2150459982","https://openalex.org/W2152896217","https://openalex.org/W2157144491","https://openalex.org/W2157646577","https://openalex.org/W2160571418","https://openalex.org/W2160712561","https://openalex.org/W2169235788","https://openalex.org/W2284101434","https://openalex.org/W2290816325","https://openalex.org/W2405310576","https://openalex.org/W2516573240"],"related_works":["https://openalex.org/W2591912505","https://openalex.org/W1510406077","https://openalex.org/W2223332214","https://openalex.org/W2073127757","https://openalex.org/W31245993","https://openalex.org/W2057744612","https://openalex.org/W2025201263","https://openalex.org/W2139496928","https://openalex.org/W2045041691","https://openalex.org/W2139183408"],"abstract_inverted_index":{"Measuring":[0],"N":[1],"ADPH":[2],"ox":[3],"idase":[4],"(Nox)-derived":[5],"r":[6],"eactive":[7],"o":[8],"xygen":[9],"s":[10],"pecies":[11],"(ROS)":[12],"in":[13,53,74,104,112,161,187,207,220,229],"living":[14],"tissues":[15],"and":[16,44,83,134,139,181,192],"cells":[17,84,96,209,246],"is":[18],"a":[19,39,119,126,158,184],"constant":[20],"challenge.":[21],"All":[22],"probes":[23],"available":[24],"display":[25],"limitations":[26],"regarding":[27],"sensitivity,":[28],"specificity":[29],"or":[30,212],"demand":[31],"highly":[32],"specialized":[33],"detection":[34],"techniques.":[35],"In":[36,272],"search":[37],"for":[38,279],"presumably":[40],"easy,":[41],"versatile,":[42],"sensitive":[43],"specific":[45],"technique,":[46],"numerous":[47],"studies":[48],"have":[49,57],"used":[50],"NADPH-stimulated":[51,105,132,162,230],"assays":[52,73,114,133,164,188,231],"membrane":[54,106,163,233],"fractions":[55,234],"which":[56,258],"been":[58],"suggested":[59],"to":[60,86,116],"reflect":[61],"Nox":[62,76,98,286],"activity.":[63],"However,":[64,223],"we":[65],"previously":[66],"found":[67],"an":[68,252],"unaltered":[69],"activity":[70,160,287],"with":[71,217,248,256],"these":[72,113],"triple":[75],"knockout":[77,263,269],"mouse":[78],"(Nox1-Nox2-Nox4":[79],"-/-":[80,205,225],")":[81,206],"tissue":[82],"compared":[85],"wild":[87],"type.":[88],"Moreover,":[89,240],"the":[90,109,148,173,197,227,236,262,274,280,283],"high":[91],"ROS":[92,218],"production":[93,219],"of":[94,128,147,172,196,242,264,270,282,285],"intact":[95,221],"overexpressing":[97,176,210],"enzymes":[99],"could":[100],"not":[101,215,267],"be":[102],"recapitulated":[103],"assays.":[107,290],"Thus,":[108],"signal":[110,186,228,255,284],"obtained":[111],"has":[115],"derive":[117],"from":[118,235],"source":[120,146],"other":[121],"than":[122],"NADPH":[123],"oxidases.":[124],"Using":[125],"combination":[127],"native":[129],"protein":[130],"electrophoresis,":[131],"mass":[135],"spectrometry,":[136],"mitochondrial":[137,154,167],"proteins":[138],"cytochrome":[140,198,275],"P450":[141,177,182,199,276],"were":[142],"identified":[143],"as":[144],"possible":[145],"assay":[149],"signal.":[150,174],"Cells":[151],"lacking":[152],"functional":[153],"complexes,":[155],"however,":[156],"displayed":[157],"normal":[159],"suggesting":[165],"that":[166],"oxidoreductases":[168],"are":[169],"unlikely":[170],"sources":[171],"Microsomes":[175],"reductase,":[178],"cytochromes":[179],"b5":[180],"generated":[183],"NADPH-dependent":[185,254],"utilizing":[189],"lucigenin,":[190],"L-012":[191],"dihydroethidium":[193],"(DHE).":[194],"Knockout":[195],"reductase":[200],"by":[201,261,268],"CRISPR/Cas9":[202],"technology":[203],"(POR":[204],"HEK293":[208],"Nox4":[211],"Nox5":[213],"did":[214],"interfere":[216],"cells.":[222,239],"POR":[224,265],"abolished":[226,260],"using":[232],"very":[237],"same":[238],"membranes":[241],"rat":[243],"smooth":[244],"muscle":[245],"treated":[247],"angiotensin":[249],"II":[250],"showed":[251],"increased":[253],"lucigenin":[257],"was":[259],"but":[266],"p22phox.":[271],"conclusion:":[273],"system":[277],"accounts":[278],"majority":[281],"chemiluminescence":[288],"based":[289]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":11},{"year":2017,"cited_by_count":5}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-11-30T00:00:00"}