{"id":"https://openalex.org/W2531660977","doi":null,"title":"Analysis of signal pathway protein-protein interactions during biotic and abiotic stress.","display_name":"Analysis of signal pathway protein-protein interactions during biotic and abiotic stress.","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W2531660977","mag":"2531660977"},"language":"en","primary_location":{"id":"pmh:oai:digital.library.adelaide.edu.au:2440/60985","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2440/60985","pdf_url":"http://hdl.handle.net/2440/60985","source":{"id":"https://openalex.org/S4306401835","display_name":"Adelaide Research & Scholarship (AR&S) (University of Adelaide)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I5681781","host_organization_name":"The University of Adelaide","host_organization_lineage":["https://openalex.org/I5681781"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/2440/60985","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038056357","display_name":"Jenna Moira Malone","orcid":"https://orcid.org/0000-0002-9637-2073","observed_orcids":["https://orcid.org/0000-0002-9637-2073"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Jenna Malone","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9637-2073","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5038056357"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.6797000169754028,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.6797000169754028,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.27000001072883606,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.015200000256299973,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/barley","display_name":"barley","score":0.8579999804496765},{"id":"https://openalex.org/keywords/rhynchosporium-secalis","display_name":"Rhynchosporium secalis","score":0.9259999990463257},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.8930000066757202},{"id":"https://openalex.org/keywords/plant-defense-signaling","display_name":"plant defense signaling","score":0.7680000066757202},{"id":"https://openalex.org/keywords/abiotic-stress-response","display_name":"abiotic stress response","score":0.7200000286102295},{"id":"https://openalex.org/keywords/compatible-and-incompatible-interactions","display_name":"compatible and incompatible interactions","score":0.5699999928474426},{"id":"https://openalex.org/keywords/epsps","display_name":"EPSPS","score":0.8769999742507935},{"id":"https://openalex.org/keywords/rab-gtpases","display_name":"Rab GTPases","score":0.8180000185966492},{"id":"https://openalex.org/keywords/gene-co-expression-network","display_name":"gene co-expression network","score":0.4020000100135803}],"concepts":[{"id":"https://openalex.org/C132215390","wikidata":"https://www.wikidata.org/wiki/Q461335","display_name":"Abiotic component","level":2,"score":0.725172758102417},{"id":"https://openalex.org/C146926016","wikidata":"https://www.wikidata.org/wiki/Q3500367","display_name":"Biotic stress","level":4,"score":0.5391343832015991},{"id":"https://openalex.org/C89519541","wikidata":"https://www.wikidata.org/wiki/Q4667893","display_name":"Abiotic stress","level":3,"score":0.5128822326660156},{"id":"https://openalex.org/C21036866","wikidata":"https://www.wikidata.org/wiki/Q181767","display_name":"Stress (linguistics)","level":2,"score":0.4424003064632416},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.3743923306465149},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36927330493927},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3341469168663025},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1189546287059784},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:digital.library.adelaide.edu.au:2440/60985","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2440/60985","pdf_url":"http://hdl.handle.net/2440/60985","source":{"id":"https://openalex.org/S4306401835","display_name":"Adelaide Research & Scholarship (AR&S) (University of Adelaide)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I5681781","host_organization_name":"The University of Adelaide","host_organization_lineage":["https://openalex.org/I5681781"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2531660977","doi":null,"is_oa":false,"landing_page_url":"https://digital.library.adelaide.edu.au/dspace/bitstream/2440/60985/9/01front.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:digital.library.adelaide.edu.au:2440/60985","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2440/60985","pdf_url":"http://hdl.handle.net/2440/60985","source":{"id":"https://openalex.org/S4306401835","display_name":"Adelaide Research & Scholarship (AR&S) (University of Adelaide)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I5681781","host_organization_name":"The University of Adelaide","host_organization_lineage":["https://openalex.org/I5681781"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[{"score":0.8409000039100647,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2531660977.pdf","grobid_xml":"https://content.openalex.org/works/W2531660977.grobid-xml"},"referenced_works_count":218,"referenced_works":["https://openalex.org/W8231427","https://openalex.org/W38542220","https://openalex.org/W61582473","https://openalex.org/W106100846","https://openalex.org/W141477066","https://openalex.org/W146014156","https://openalex.org/W275357379","https://openalex.org/W276538718","https://openalex.org/W657118046","https://openalex.org/W996801350","https://openalex.org/W1237714677","https://openalex.org/W1495919075","https://openalex.org/W1499500424","https://openalex.org/W1500274797","https://openalex.org/W1509303071","https://openalex.org/W1517210188","https://openalex.org/W1521430927","https://openalex.org/W1548379687","https://openalex.org/W1555799156","https://openalex.org/W1557712139","https://openalex.org/W1569287373","https://openalex.org/W1569955072","https://openalex.org/W1577577364","https://openalex.org/W1580245424","https://openalex.org/W1586271481","https://openalex.org/W1587681321","https://openalex.org/W1599420452","https://openalex.org/W1608933537","https://openalex.org/W1748210090","https://openalex.org/W1760154841","https://openalex.org/W1809644882","https://openalex.org/W1810371350","https://openalex.org/W1909852369","https://openalex.org/W1955139281","https://openalex.org/W1965266364","https://openalex.org/W1966048054","https://openalex.org/W1966453639","https://openalex.org/W1969284236","https://openalex.org/W1969971113","https://openalex.org/W1970707895","https://openalex.org/W1971034474","https://openalex.org/W1971527377","https://openalex.org/W1972700083","https://openalex.org/W1975622239","https://openalex.org/W1975866949","https://openalex.org/W1977186059","https://openalex.org/W1978666863","https://openalex.org/W1978892165","https://openalex.org/W1979584705","https://openalex.org/W1980142178","https://openalex.org/W1980823210","https://openalex.org/W1982177986","https://openalex.org/W1982511335","https://openalex.org/W1982651764","https://openalex.org/W1982879218","https://openalex.org/W1985605910","https://openalex.org/W1986146727","https://openalex.org/W1987486987","https://openalex.org/W1987894686","https://openalex.org/W1989629234","https://openalex.org/W1990632516","https://openalex.org/W1991557803","https://openalex.org/W1995176042","https://openalex.org/W1996133279","https://openalex.org/W1996405087","https://openalex.org/W1996473145","https://openalex.org/W1997848611","https://openalex.org/W2002275715","https://openalex.org/W2002478697","https://openalex.org/W2003520738","https://openalex.org/W2004335389","https://openalex.org/W2004513287","https://openalex.org/W2005500718","https://openalex.org/W2005905134","https://openalex.org/W2008183439","https://openalex.org/W2008406318","https://openalex.org/W2010130827","https://openalex.org/W2011532886","https://openalex.org/W2011954737","https://openalex.org/W2013452582","https://openalex.org/W2013840519","https://openalex.org/W2014494440","https://openalex.org/W2015350698","https://openalex.org/W2015573735","https://openalex.org/W2016613744","https://openalex.org/W2016629089","https://openalex.org/W2016772922","https://openalex.org/W2018432365","https://openalex.org/W2019540930","https://openalex.org/W2020942562","https://openalex.org/W2021077280","https://openalex.org/W2021266779","https://openalex.org/W2021268189","https://openalex.org/W2023987813","https://openalex.org/W2024923170","https://openalex.org/W2025459473","https://openalex.org/W2029429040","https://openalex.org/W2029523792","https://openalex.org/W2030248614","https://openalex.org/W2030614920","https://openalex.org/W2030626904","https://openalex.org/W2034368931","https://openalex.org/W2034784092","https://openalex.org/W2036161032","https://openalex.org/W2037227939","https://openalex.org/W2038100335","https://openalex.org/W2038758518","https://openalex.org/W2047057557","https://openalex.org/W2047308608","https://openalex.org/W2048570688","https://openalex.org/W2049125435","https://openalex.org/W2052218725","https://openalex.org/W2054248545","https://openalex.org/W2054464810","https://openalex.org/W2055043387","https://openalex.org/W2057177793","https://openalex.org/W2058539798","https://openalex.org/W2061439657","https://openalex.org/W2062883953","https://openalex.org/W2064669866","https://openalex.org/W2064943475","https://openalex.org/W2066236069","https://openalex.org/W2066314966","https://openalex.org/W2068700065","https://openalex.org/W2068897525","https://openalex.org/W2068924803","https://openalex.org/W2069319059","https://openalex.org/W2069361154","https://openalex.org/W2070721758","https://openalex.org/W2071075259","https://openalex.org/W2071816183","https://openalex.org/W2073906262","https://openalex.org/W2074250505","https://openalex.org/W2074432872","https://openalex.org/W2074487975","https://openalex.org/W2077452450","https://openalex.org/W2078788276","https://openalex.org/W2078895980","https://openalex.org/W2079153043","https://openalex.org/W2079167727","https://openalex.org/W2081540595","https://openalex.org/W2082062508","https://openalex.org/W2082221845","https://openalex.org/W2082987551","https://openalex.org/W2083240338","https://openalex.org/W2083527717","https://openalex.org/W2083660896","https://openalex.org/W2085888856","https://openalex.org/W2087644087","https://openalex.org/W2088973286","https://openalex.org/W2089148335","https://openalex.org/W2090685194","https://openalex.org/W2092151672","https://openalex.org/W2094131214","https://openalex.org/W2094354737","https://openalex.org/W2094411158","https://openalex.org/W2100168566","https://openalex.org/W2100658001","https://openalex.org/W2100783195","https://openalex.org/W2102227865","https://openalex.org/W2104069177","https://openalex.org/W2106797025","https://openalex.org/W2106857639","https://openalex.org/W2107233773","https://openalex.org/W2108388593","https://openalex.org/W2108833346","https://openalex.org/W2111464410","https://openalex.org/W2111778006","https://openalex.org/W2113617470","https://openalex.org/W2115966753","https://openalex.org/W2120006314","https://openalex.org/W2120053145","https://openalex.org/W2121253860","https://openalex.org/W2121553505","https://openalex.org/W2123769370","https://openalex.org/W2124210450","https://openalex.org/W2125107199","https://openalex.org/W2126102214","https://openalex.org/W2130248437","https://openalex.org/W2131090020","https://openalex.org/W2133756628","https://openalex.org/W2135259591","https://openalex.org/W2136695833","https://openalex.org/W2136869738","https://openalex.org/W2138032440","https://openalex.org/W2138201584","https://openalex.org/W2138883423","https://openalex.org/W2139549901","https://openalex.org/W2142082526","https://openalex.org/W2143146123","https://openalex.org/W2143316408","https://openalex.org/W2146805707","https://openalex.org/W2150273852","https://openalex.org/W2150352010","https://openalex.org/W2151721001","https://openalex.org/W2152972832","https://openalex.org/W2153594108","https://openalex.org/W2157058776","https://openalex.org/W2157510518","https://openalex.org/W2157815481","https://openalex.org/W2158110386","https://openalex.org/W2158597576","https://openalex.org/W2160313474","https://openalex.org/W2163498840","https://openalex.org/W2164054146","https://openalex.org/W2164578725","https://openalex.org/W2165205273","https://openalex.org/W2165963364","https://openalex.org/W2169615676","https://openalex.org/W2170106992","https://openalex.org/W2170288917","https://openalex.org/W2293835823","https://openalex.org/W2319971084","https://openalex.org/W2323705175","https://openalex.org/W2408111051","https://openalex.org/W2410961934","https://openalex.org/W2469005906","https://openalex.org/W3187594710"],"related_works":["https://openalex.org/W2106797025","https://openalex.org/W2091406164","https://openalex.org/W25358616","https://openalex.org/W2918511391","https://openalex.org/W7095568320","https://openalex.org/W1965799425","https://openalex.org/W2090722361","https://openalex.org/W7100169905","https://openalex.org/W2949336942","https://openalex.org/W2166718066"],"abstract_inverted_index":{"The":[0,135],"overall":[1],"objective":[2],"of":[3,22,46,81,103,114,137,160,171,258,272,289,327,377,390,450,474,518],"the":[4,13,49,58,101,138,143,157,169,200,210,234,278,287,293,306,315,325,366,370,378,388,395,398,402,413,440,448,454,467,472,516],"work":[5],"described":[6],"in":[7,20,25,41,57,73,147,156,168,207,229,292,301,311,387,432,444,463,506],"this":[8,358,451],"thesis":[9],"was":[10,128,250,342,424],"to":[11,37,94,189,225,232,253,308,406,427,438],"characterise":[12,95],"three":[14,403],"genes":[15,32,67,145,204,235,291,396,456,488,497],"Hv14.3.3c,":[16,457],"HvMAPKK1":[17,458],"and":[18,27,100,123,174,179,220,241,243,264,269,277,305,319,383,401,417,434,459,493,510],"HvFKBP41,":[19],"terms":[21],"a":[23,54,71,78,154,237,265,274,299,333,408,502],"role":[24,56,72,155,183,300,505],"defence":[26,60,158,211,507],"stress":[28,195,302,521],"response":[29,159,303,508],"signalling.":[30],"These":[31],"had":[33],"previously":[34,475],"been":[35,483],"found":[36],"be":[38,186,309,512],"differentially":[39,149],"expressed":[40,150],"compatible":[42],"versus":[43],"incompatible":[44],"interactions":[45,367],"barley":[47,161],"with":[48,298,314,321,347,429],"fungus":[50],"Rhynchosporium":[51],"secalis,":[52],"suggesting":[53,153],"possible":[55],"plant":[59,340,468],"response,":[61,212],"while":[62],"current":[63],"literature":[64],"suggests":[65],"these":[66,203,246,290,487],"may":[68,184],"also":[69,320,482],"play":[70,501],"signal":[74,464],"transduction,":[75],"possibly":[76,500],"under":[77],"broad":[79],"range":[80],"stresses,":[82],"including":[83],"abiotic":[84,194],"as":[85,87,196,217,461],"well":[86,297],"biotic.":[88],"\\nTwo":[89],"main":[90],"approaches":[91],"were":[92,117,146,223,354,361,381,419],"undertaken":[93],"gene":[96,116,317],"function:":[97],"expression":[98,108,139,167,384],"analysis":[99,140,385,442],"identification":[102,349,473],"protein-protein":[104,213],"interactions.":[105],"\\nTo":[106,198,411],"facilitate":[107],"analysis,":[109],"full":[110],"length":[111,374],"cDNA":[112,375],"fragments":[113],"each":[115,322],"first":[118],"obtained":[119,382],"using":[120,131,357,369],"bioinformatics,":[121],"RACE":[122],"genomic":[124],"walking":[125],"techniques.":[126],"Expression":[127],"then":[129,362,420],"investigated":[130],"quantitative":[132],"real-time":[133],"RT-PCR.":[134],"results":[136,449],"confirmed":[141],"that":[142,202,351,498],"candidate":[144,316,404,455],"fact":[148],"during":[151,209,466],"infection,":[152],"against":[162],"R.":[163],"secalis.":[164],"Analysing":[165],"their":[166,182],"context":[170],"other":[172],"stresses":[173],"treatments,":[175],"namely":[176],"frost,":[177],"drought":[178],"ABA,":[180],"indicated":[181],"not":[185,355],"limited":[187],"only":[188],"biotic":[190],"stress,":[191],"but":[192],"include":[193],"well.":[197],"investigate":[199],"possibility":[201],"are":[205],"involved":[206,310],"signalling":[208,239,304,312,491,509],"interaction":[214],"techniques":[215],"such":[216],"yeast":[218],"two-hybrid":[219],"affinity":[221,330],"pulldowns":[222],"used":[224,251],"identify":[226,254],"interacting":[227,337,352,399],"proteins":[228,338,353,400],"an":[230,260],"attempt":[231],"place":[233,453],"within":[236,489],"known":[238,285,490],"network":[240],"build":[242],"extend":[244],"on":[245],"networks.":[247],"\\nY2H":[248],"screening":[249],"successfully":[252,343],"two":[255,270],"putative":[256,266],"interactors":[257,271,380],"Hv14.3.3c;":[259],"EPSP":[261],"(5-enolpyruvylshikimate-3-phosphate)":[262],"synthase":[263],"wound-induced":[267,280],"protein,":[268],"HvFKBP41;":[273],"Rab-type":[275],"GTPase":[276],"same":[279],"protein.":[281],"From":[282],"what":[283],"is":[284],"about":[286],"function":[288],"literature,":[294],"they":[295],"fit":[296],"potential":[307,409,495,513],"networks":[313,492],"products":[318],"other.":[323],"\\nThrough":[324],"trial":[326],"many":[328],"different":[329],"pulldown":[331],"techniques,":[332],"method":[334],"for":[335,515],"identifying":[336,494],"from":[339],"extracts":[341],"established,":[344],"however,":[345],"issues":[346,428,436],"protein":[348,477],"meant":[350],"identified":[356,368,379],"technique.":[359],"\\nSteps":[360],"made":[363,484],"towards":[364,485],"confirming":[365],"Y2H":[371],"system.":[372],"Full":[373],"sequences":[376],"performed,":[386],"aim":[389],"investigating":[391],"co-expression":[392],"patterns":[393],"between":[394],"encoding":[397],"genes,":[405],"support":[407],"interaction.":[410],"confirm":[412],"Hv14.3.3c-HvEPSP":[414],"interaction,":[415],"co-immunoprecipitation":[416,433],"BRET":[418,441],"used,":[421],"however":[422],"confirmation":[423],"unsuccessful":[425],"due":[426],"non-specific":[430],"binding":[431],"technical":[435],"trying":[437],"establish":[439],"system":[443],"barley.":[445],"\\nIn":[446],"summary,":[447],"study":[452],"HvFKBP41":[460],"players":[462],"transduction":[465],"defence/stress":[469],"response.":[470],"With":[471],"uncharacterised":[476],"interactions,":[478],"some":[479],"progress":[480],"has":[481],"placing":[486],"downstream":[496],"could":[499],"more":[503],"specific":[504],"therefore":[511],"targets":[514],"generation":[517],"resistant":[519],"or":[520],"tolerant":[522],"plants.":[523]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-10-21T00:00:00"}