{"id":"https://openalex.org/W2516355239","doi":"https://doi.org/10.1038/srep31782","title":"Increasing the performance of pooled CRISPR\u2013Cas9 drop-out screening","display_name":"Increasing the performance of pooled CRISPR\u2013Cas9 drop-out screening","publication_year":2016,"publication_date":"2016-08-22","ids":{"openalex":"https://openalex.org/W2516355239","doi":"https://doi.org/10.1038/srep31782","mag":"2516355239","pmid":"https://pubmed.ncbi.nlm.nih.gov/27545104","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4992892"},"language":"en","primary_location":{"id":"doi:10.1038/srep31782","doi":"https://doi.org/10.1038/srep31782","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep31782","pdf_url":"https://www.nature.com/articles/srep31782.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/srep31782.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011709620","display_name":"Benedict C. S. Cross","orcid":"https://orcid.org/0000-0003-1065-3139","observed_orcids":["https://orcid.org/0000-0003-1065-3139"]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Benedict C. S. Cross","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025167323","display_name":"Steffen Lawo","orcid":"https://orcid.org/0000-0002-9659-7416","observed_orcids":["https://orcid.org/0000-0002-9659-7416"]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Steffen Lawo","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086935273","display_name":"Caroline R. Archer","orcid":"https://orcid.org/0000-0001-6273-7153","observed_orcids":["https://orcid.org/0000-0001-6273-7153"]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Caroline R. Archer","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026646144","display_name":"Jessica R. Hunt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jessica R. Hunt","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063391157","display_name":"Joanne L. Yarker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Joanne L. Yarker","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029274543","display_name":"Alessandro Riccombeni","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Alessandro Riccombeni","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024839265","display_name":"Annette S. Little","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Annette S. Little","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039242992","display_name":"Nicola McCarthy","orcid":"https://orcid.org/0000-0001-6990-3943","observed_orcids":["https://orcid.org/0000-0001-6990-3943","https://orcid.org/0000-0003-4204-731X"]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Nicola J. McCarthy","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5064129953","display_name":"Jonathan D. Moore","orcid":"https://orcid.org/0000-0002-5486-0407","observed_orcids":["https://orcid.org/0000-0002-5486-0407"]},"institutions":[{"id":"https://openalex.org/I4210095126","display_name":"Horizon Discovery (United Kingdom)","ror":"https://ror.org/00q2y5s03","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210095126","https://openalex.org/I43591049","https://openalex.org/I4405255244"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jonathan D. Moore","raw_affiliation_strings":["Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]},{"raw_affiliation_string":"Horizon Discovery, 8100 Cambridge Research Park, Waterbeach, CB25 9TL, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I4210095126"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5011709620"],"corresponding_institution_ids":["https://openalex.org/I4210095126"],"apc_list":{"value":2690,"currency":"USD","value_usd":2690},"apc_paid":{"value":2690,"currency":"USD","value_usd":2690},"fwci":1.2803,"has_fulltext":true,"cited_by_count":38,"citation_normalized_percentile":{"value":0.79150329,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"6","issue":"1","first_page":"31782","last_page":"31782"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.9710000157356262},{"id":"https://openalex.org/keywords/guide-rna-design","display_name":"guide RNA design","score":0.625},{"id":"https://openalex.org/keywords/sgrna-library","display_name":"sgRNA library","score":0.6549999713897705},{"id":"https://openalex.org/keywords/essential-genes","display_name":"essential genes","score":0.8119999766349792},{"id":"https://openalex.org/keywords/drug-target-validation","display_name":"drug target validation","score":0.7749999761581421}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9373996257781982},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.6177351474761963},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5988447666168213},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4545038342475891},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.4436013698577881},{"id":"https://openalex.org/C2909335157","wikidata":"https://www.wikidata.org/wiki/Q17162809","display_name":"Screening techniques","level":2,"score":0.4388901889324188},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.42563486099243164},{"id":"https://openalex.org/C63479239","wikidata":"https://www.wikidata.org/wiki/Q7353546","display_name":"Robustness (evolution)","level":3,"score":0.4240087866783142},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42395728826522827},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3680174648761749},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2957514524459839},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.2879527509212494}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005820","descriptor_name":"Genetic Testing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018390","descriptor_name":"Gene Targeting","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018922","descriptor_name":"HL-60 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1038/srep31782","doi":"https://doi.org/10.1038/srep31782","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep31782","pdf_url":"https://www.nature.com/articles/srep31782.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4992892","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4992892","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:27545104","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27545104","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:www.repository.cam.ac.uk:1810/386710","doi":null,"is_oa":true,"landing_page_url":"https://www.repository.cam.ac.uk/handle/1810/386710","pdf_url":null,"source":{"id":"https://openalex.org/S4306401777","display_name":"Apollo (University of Cambridge)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I241749","host_organization_name":"University of Cambridge","host_organization_lineage":["https://openalex.org/I241749"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1038/srep31782","doi":"https://doi.org/10.1038/srep31782","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep31782","pdf_url":"https://www.nature.com/articles/srep31782.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2516355239.pdf","grobid_xml":"https://content.openalex.org/works/W2516355239.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W1594078235","https://openalex.org/W1965404910","https://openalex.org/W1973260880","https://openalex.org/W2026592619","https://openalex.org/W2031211461","https://openalex.org/W2045435533","https://openalex.org/W2050480410","https://openalex.org/W2063148079","https://openalex.org/W2066422597","https://openalex.org/W2068427957","https://openalex.org/W2100122648","https://openalex.org/W2118967129","https://openalex.org/W2180291937","https://openalex.org/W2201689894","https://openalex.org/W2210272252","https://openalex.org/W2338215681","https://openalex.org/W2342536142"],"related_works":["https://openalex.org/W4407973731","https://openalex.org/W2396039786","https://openalex.org/W2809390538","https://openalex.org/W1594078235","https://openalex.org/W4361256010","https://openalex.org/W3110631837","https://openalex.org/W2412521064","https://openalex.org/W4312213638","https://openalex.org/W2896929167","https://openalex.org/W2983296860"],"abstract_inverted_index":{"Components":[0],"of":[1,47,70,84,92,105,112,142,149,163],"the":[2,45,82,110,143,147,158],"type":[3],"II":[4],"CRISPR-Cas":[5,52],"complex":[6],"in":[7,13,67,114,131],"bacteria":[8],"have":[9],"been":[10],"used":[11],"successfully":[12],"eukaryotic":[14],"cells":[15],"to":[16,63,75],"facilitate":[17],"rapid":[18],"and":[19,27,41,74,117,127,171],"accurate":[20],"cell":[21,72,119],"line":[22],"engineering,":[23],"animal":[24],"model":[25],"generation":[26],"functional":[28],"genomic":[29],"screens.":[30],"Such":[31],"developments":[32],"are":[33],"providing":[34],"new":[35,56,175],"opportunities":[36],"for":[37,160,177],"drug":[38],"target":[39],"identification":[40],"validation,":[42],"particularly":[43],"with":[44],"application":[46],"pooled":[48],"genetic":[49,57],"screening.":[50,179],"As":[51],"is":[53,61],"a":[54,68,85,103,132,139,153,173],"relatively":[55],"screening":[58,93,113],"tool,":[59],"it":[60],"important":[62],"assess":[64],"its":[65],"functionality":[66],"number":[69],"different":[71],"lines":[73],"analyse":[76],"potential":[77],"improvements":[78],"that":[79,138],"might":[80],"increase":[81],"sensitivity":[83],"given":[86],"screen.":[87,154],"To":[88],"examine":[89],"critical":[90],"aspects":[91],"quality,":[94],"we":[95],"constructed":[96],"ultra-complex":[97],"libraries":[98],"containing":[99],"sgRNA":[100],"sequences":[101],"targeting":[102,165],"collection":[104],"essential":[106],"genes.":[107],"We":[108],"examined":[109],"performance":[111],"both":[115],"haploid":[116],"hypotriploid":[118],"lines,":[120],"using":[121],"two":[122,128],"alternative":[123],"guide":[124,150],"design":[125],"algorithms":[126],"tracrRNA":[129,144],"variants":[130],"time-resolved":[133],"analysis.":[134],"Our":[135],"data":[136],"indicate":[137],"simple":[140],"adaptation":[141],"substantially":[145],"improves":[146],"robustness":[148],"loss":[151],"during":[152],"This":[155],"modification":[156],"minimises":[157],"requirement":[159],"high":[161],"numbers":[162],"sgRNAs":[164],"each":[166],"gene,":[167],"increasing":[168],"hit":[169],"scoring":[170],"creating":[172],"powerful":[174],"platform":[176],"successful":[178]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-09-16T00:00:00"}
