{"id":"https://openalex.org/W2515083702","doi":"https://doi.org/10.3390/ijms17091507","title":"Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview","display_name":"Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview","publication_year":2016,"publication_date":"2016-09-08","ids":{"openalex":"https://openalex.org/W2515083702","doi":"https://doi.org/10.3390/ijms17091507","mag":"2515083702","pmid":"https://pubmed.ncbi.nlm.nih.gov/27618019","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5037784"},"language":"en","primary_location":{"id":"doi:10.3390/ijms17091507","doi":"https://doi.org/10.3390/ijms17091507","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms17091507","pdf_url":null,"source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3390/ijms17091507","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044896776","display_name":"Francisco Mart\u0131\u0301n","orcid":"https://orcid.org/0000-0003-1961-8612","observed_orcids":["https://orcid.org/0000-0003-1961-8612"]},"institutions":[{"id":"https://openalex.org/I173304897","display_name":"Universidad de Granada","ror":"https://ror.org/04njjy449","country_code":"ES","type":"education","lineage":["https://openalex.org/I173304897"]},{"id":"https://openalex.org/I4210142177","display_name":"Pfizer-University of Granada-Junta de Andaluc\u00eda Centre for Genomics and Oncological Research","ror":"https://ror.org/04hr99439","country_code":"ES","type":"facility","lineage":["https://openalex.org/I4210142177"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"Francisco Martin","raw_affiliation_strings":["Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","LentiStem Biotech, GENYO, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","institution_ids":["https://openalex.org/I173304897","https://openalex.org/I4210142177"]},{"raw_affiliation_string":"LentiStem Biotech, GENYO, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","institution_ids":["https://openalex.org/I4210142177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011493462","display_name":"Sabina S\u00e1nchez-Hern\u00e1ndez","orcid":"https://orcid.org/0000-0002-5858-4139","observed_orcids":["https://orcid.org/0000-0002-5858-4139"]},"institutions":[{"id":"https://openalex.org/I173304897","display_name":"Universidad de Granada","ror":"https://ror.org/04njjy449","country_code":"ES","type":"education","lineage":["https://openalex.org/I173304897"]},{"id":"https://openalex.org/I4210142177","display_name":"Pfizer-University of Granada-Junta de Andaluc\u00eda Centre for Genomics and Oncological Research","ror":"https://ror.org/04hr99439","country_code":"ES","type":"facility","lineage":["https://openalex.org/I4210142177"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Sabina S\u00e1nchez-Hern\u00e1ndez","raw_affiliation_strings":["Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","institution_ids":["https://openalex.org/I173304897","https://openalex.org/I4210142177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024299591","display_name":"Alejandra Guti\u00e9rrez-Guerrero","orcid":"https://orcid.org/0000-0002-3966-9225","observed_orcids":["https://orcid.org/0000-0002-3966-9225","https://orcid.org/0000-0003-4519-1501"]},"institutions":[{"id":"https://openalex.org/I173304897","display_name":"Universidad de Granada","ror":"https://ror.org/04njjy449","country_code":"ES","type":"education","lineage":["https://openalex.org/I173304897"]},{"id":"https://openalex.org/I4210142177","display_name":"Pfizer-University of Granada-Junta de Andaluc\u00eda Centre for Genomics and Oncological Research","ror":"https://ror.org/04hr99439","country_code":"ES","type":"facility","lineage":["https://openalex.org/I4210142177"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Alejandra Guti\u00e9rrez-Guerrero","raw_affiliation_strings":["Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","institution_ids":["https://openalex.org/I173304897","https://openalex.org/I4210142177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039099300","display_name":"Javier Pinedo-Gomez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173304897","display_name":"Universidad de Granada","ror":"https://ror.org/04njjy449","country_code":"ES","type":"education","lineage":["https://openalex.org/I173304897"]},{"id":"https://openalex.org/I4210142177","display_name":"Pfizer-University of Granada-Junta de Andaluc\u00eda Centre for Genomics and Oncological Research","ror":"https://ror.org/04hr99439","country_code":"ES","type":"facility","lineage":["https://openalex.org/I4210142177"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Javier Pinedo-Gomez","raw_affiliation_strings":["Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","institution_ids":["https://openalex.org/I173304897","https://openalex.org/I4210142177"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5020930599","display_name":"Karim Benabdellah","orcid":"https://orcid.org/0000-0003-4673-2111","observed_orcids":["https://orcid.org/0000-0003-4673-2111"]},"institutions":[{"id":"https://openalex.org/I173304897","display_name":"Universidad de Granada","ror":"https://ror.org/04njjy449","country_code":"ES","type":"education","lineage":["https://openalex.org/I173304897"]},{"id":"https://openalex.org/I4210142177","display_name":"Pfizer-University of Granada-Junta de Andaluc\u00eda Centre for Genomics and Oncological Research","ror":"https://ror.org/04hr99439","country_code":"ES","type":"facility","lineage":["https://openalex.org/I4210142177"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"Karim Benabdellah","raw_affiliation_strings":["Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","LentiStem Biotech, GENYO, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain"],"raw_orcid":"https://orcid.org/0000-0003-4673-2111","affiliations":[{"raw_affiliation_string":"Genomic Medicine Department, GENYO\u2014Centre for Genomics and Oncological Research Pfizer-Universidad de Granada-Junta de Andaluc\u00eda, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","institution_ids":["https://openalex.org/I173304897","https://openalex.org/I4210142177"]},{"raw_affiliation_string":"LentiStem Biotech, GENYO, Avda de la Ilustraci\u00f3n 114, 18007 Granada, Spain","institution_ids":["https://openalex.org/I4210142177"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5020930599","https://openalex.org/A5044896776"],"corresponding_institution_ids":["https://openalex.org/I173304897","https://openalex.org/I4210142177"],"apc_list":{"value":2900,"currency":"CHF","value_usd":3499},"apc_paid":{"value":2900,"currency":"CHF","value_usd":3499},"fwci":0.6929,"has_fulltext":true,"cited_by_count":92,"citation_normalized_percentile":{"value":0.58098797,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"17","issue":"9","first_page":"1507","last_page":"1507"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11491","display_name":"CAR-T cell therapy research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.9950000047683716},{"id":"https://openalex.org/keywords/off-target-cleavage","display_name":"off-target cleavage","score":0.9599999785423279},{"id":"https://openalex.org/keywords/off-target-detection","display_name":"off-target detection","score":0.8309999704360962},{"id":"https://openalex.org/keywords/dna-double-strand-breaks","display_name":"DNA double-strand breaks","score":0.8519999980926514},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.8149999976158142},{"id":"https://openalex.org/keywords/zinc-finger-nucleases","display_name":"zinc finger nucleases","score":0.703000009059906},{"id":"https://openalex.org/keywords/transcription-activator-like-effector-nucleases","display_name":"TALEN","score":0.9520000219345093}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9848681688308716},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.8632852435112},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.7370779514312744},{"id":"https://openalex.org/C31909778","wikidata":"https://www.wikidata.org/wiki/Q7833921","display_name":"Transcription activator-like effector nuclease","level":5,"score":0.694830060005188},{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.6897078156471252},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.615592360496521},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5586475133895874},{"id":"https://openalex.org/C44667518","wikidata":"https://www.wikidata.org/wiki/Q12321","display_name":"Palindrome","level":4,"score":0.5476894378662109},{"id":"https://openalex.org/C2992406208","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"Double strand","level":4,"score":0.5039505362510681},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.42279553413391113},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3430502414703369},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20267564058303833},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.164203941822052}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015982","descriptor_name":"Bias","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018390","descriptor_name":"Gene Targeting","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018390","descriptor_name":"Gene Targeting","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.3390/ijms17091507","doi":"https://doi.org/10.3390/ijms17091507","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms17091507","pdf_url":null,"source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:5037784","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5037784","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Int J Mol Sci","raw_type":"Text"},{"id":"pmid:27618019","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27618019","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of molecular sciences","raw_type":"Journal Article"},{"id":"pmh:oai:www.repositoriosalud.es:10668/19271","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10668/19271","pdf_url":"https://www.mdpi.com/1422-0067/17/9/1507/pdf?version=1473331598","source":{"id":"https://openalex.org/S4377196114","display_name":"RISalud Institutional Health Repository of Andaluc\u00eda","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"review"},{"id":"pmh:oai:doaj.org/article:2c7c3d77e68c47d1981f38faa8f1c745","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/2c7c3d77e68c47d1981f38faa8f1c745","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"International Journal of Molecular Sciences, Vol 17, Iss 9, p 1507 (2016)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/ijms17091507","doi":"https://doi.org/10.3390/ijms17091507","is_oa":true,"landing_page_url":"https://doi.org/10.3390/ijms17091507","pdf_url":null,"source":{"id":"https://openalex.org/S10623703","display_name":"International Journal of Molecular Sciences","issn_l":"1422-0067","issn":["1422-0067","1661-6596"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Molecular Sciences","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2661508407","display_name":null,"funder_award_id":"PI12/01097","funder_id":"https://openalex.org/F4320334923","funder_display_name":"Instituto de Salud Carlos III"},{"id":"https://openalex.org/G4455420815","display_name":"Edici\u00f3n g\u00e9nica como alternativa a los vectores retrovirales para terapia g\u00e9nica del s\u00edndrome de Wiskott-Aldrich","funder_award_id":"PI15/02015","funder_id":"https://openalex.org/F4320334923","funder_display_name":"Instituto de Salud Carlos III"},{"id":"https://openalex.org/G5053627382","display_name":null,"funder_award_id":"RD12/0019/0006","funder_id":"https://openalex.org/F4320334923","funder_display_name":"Instituto de Salud Carlos III"},{"id":"https://openalex.org/G575896470","display_name":null,"funder_award_id":"PI15/02015","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G7883824113","display_name":null,"funder_award_id":"RD12/0019/0006","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G8315701391","display_name":null,"funder_award_id":"grants PI12/01097","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G8633004816","display_name":null,"funder_award_id":"PI12/01097","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"}],"funders":[{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320321562","display_name":"Consejer\u00eda de Econom\u00eda, Innovaci\u00f3n, Ciencia y Empleo, Junta de Andaluc\u00eda","ror":"https://ror.org/03kak1729"},{"id":"https://openalex.org/F4320326754","display_name":"Junta de Andaluc\u00eda","ror":"https://ror.org/01jem9c82"},{"id":"https://openalex.org/F4320334923","display_name":"Instituto de Salud Carlos III","ror":"https://ror.org/00ca2c886"},{"id":"https://openalex.org/F4320335322","display_name":"European Regional Development Fund","ror":"https://ror.org/00k4n6c32"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2515083702.pdf","grobid_xml":"https://content.openalex.org/works/W2515083702.grobid-xml"},"referenced_works_count":39,"referenced_works":["https://openalex.org/W1496067884","https://openalex.org/W1555684022","https://openalex.org/W1608084731","https://openalex.org/W1922040927","https://openalex.org/W1970617888","https://openalex.org/W1974343902","https://openalex.org/W1990487336","https://openalex.org/W1997281938","https://openalex.org/W1999218372","https://openalex.org/W2003797386","https://openalex.org/W2006373538","https://openalex.org/W2027527792","https://openalex.org/W2028030005","https://openalex.org/W2030994161","https://openalex.org/W2036176224","https://openalex.org/W2039705224","https://openalex.org/W2045435533","https://openalex.org/W2049490177","https://openalex.org/W2050480410","https://openalex.org/W2051255304","https://openalex.org/W2064815984","https://openalex.org/W2077190448","https://openalex.org/W2077659966","https://openalex.org/W2080466240","https://openalex.org/W2085993152","https://openalex.org/W2105464583","https://openalex.org/W2115603949","https://openalex.org/W2125764747","https://openalex.org/W2132401721","https://openalex.org/W2148050025","https://openalex.org/W2162049340","https://openalex.org/W2167380395","https://openalex.org/W2171111703","https://openalex.org/W2172425918","https://openalex.org/W2247762492","https://openalex.org/W2258624945","https://openalex.org/W2375351600","https://openalex.org/W2466552150","https://openalex.org/W4245053291"],"related_works":["https://openalex.org/W4311579433","https://openalex.org/W2565555627","https://openalex.org/W3084464175","https://openalex.org/W2173737475","https://openalex.org/W3177585201","https://openalex.org/W2274986902","https://openalex.org/W1862162016","https://openalex.org/W2923204578","https://openalex.org/W4250775106","https://openalex.org/W2755691732"],"abstract_inverted_index":{"The":[0,72],"clustered":[1],"regularly":[2],"interspaced":[3],"short":[4],"palindromic":[5],"repeat":[6],"(CRISPR)-associated":[7],"protein":[8],"9":[9],"endonuclease":[10],"(Cas9)":[11],"derived":[12],"from":[13,69],"bacterial":[14],"adaptive":[15],"immune":[16],"systems":[17,58,131],"is":[18,30],"a":[19,31],"revolutionary":[20],"tool":[21],"used":[22],"in":[23,76,114,138],"both":[24],"basic":[25],"and":[26,132],"applied":[27],"science.":[28],"It":[29],"versatile":[32],"system":[33,105],"that":[34,66,85],"enables":[35],"the":[36,56,79,103,121],"genome":[37],"of":[38,55,74,102],"different":[39,122],"species":[40],"to":[41,87,125],"be":[42,88],"modified":[43],"by":[44,129],"generating":[45],"double":[46],"strand":[47],"breaks":[48],"(DSBs)":[49],"at":[50,63],"specific":[51,134],"locations.":[52],"However,":[53,99],"all":[54],"CRISPR/Cas9":[57,104],"can":[59,82],"also":[60],"produce":[61,83],"DSBs":[62,75],"off-target":[64,100],"sites":[65],"differ":[67],"substantially":[68],"on-target":[70],"sites.":[71],"generation":[73],"locations":[77],"outside":[78],"intended":[80],"site":[81],"mutations":[84],"need":[86],"carefully":[89],"monitored,":[90],"especially":[91],"when":[92,111],"using":[93],"these":[94],"tools":[95],"for":[96],"therapeutic":[97],"purposes.":[98],"analyses":[101],"have":[106],"been":[107],"very":[108],"challenging,":[109],"particularly":[110],"performed":[112],"directly":[113],"cells.":[115,141],"In":[116],"this":[117],"manuscript,":[118],"we":[119],"review":[120],"strategies":[123],"developed":[124],"identify":[126],"off-targets":[127],"generated":[128],"CRISPR/cas9":[130],"other":[133],"nucleases":[135],"(ZFNs,":[136],"TALENs)":[137],"real":[139],"target":[140]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":10},{"year":2023,"cited_by_count":10},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":16},{"year":2020,"cited_by_count":15},{"year":2019,"cited_by_count":8},{"year":2018,"cited_by_count":12},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-09-16T00:00:00"}