{"id":"https://openalex.org/W2509814977","doi":null,"title":"Characterization of Umbilical Cord-derived Stem Cells during Expansion in Vitro","display_name":"Characterization of Umbilical Cord-derived Stem Cells during Expansion in Vitro","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W2509814977","mag":"2509814977"},"language":"en","primary_location":{"id":"mag:2509814977","doi":null,"is_oa":false,"landing_page_url":"https://koreascience.or.kr/article/JAKO200921640757622.page","pdf_url":null,"source":{"id":"https://openalex.org/S2764373271","display_name":"Daehan saengsik uihak hoeji/Clinical and experimental reproductive medicine","issn_l":"2093-8896","issn":["2093-8896","2233-8233","2233-8241"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","kci-registered","poland-70","russia-white-list-2"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Daehan saengsik uihak hoeji/Clinical and experimental reproductive medicine","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076050154","display_name":"Seah Park","orcid":"https://orcid.org/0009-0002-9504-709X","observed_orcids":["https://orcid.org/0009-0002-9504-709X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Seah Park","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015080345","display_name":"Hyun Mi Kang","orcid":"https://orcid.org/0000-0002-2483-0776","observed_orcids":["https://orcid.org/0000-0002-2483-0776"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hyun Mi Kang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-2483-0776","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073782010","display_name":"Jinyeong Heo","orcid":"https://orcid.org/0000-0002-8180-9658","observed_orcids":["https://orcid.org/0000-0002-8180-9658"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jinyeong Heo","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8180-9658","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009486200","display_name":"Jinah Yoon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jinah Yoon","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5016342016","display_name":"Haekwon Kim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98600543","display_name":"Seoul Women's University","ror":"https://ror.org/04b2fhx54","country_code":"KR","type":"education","lineage":["https://openalex.org/I98600543"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Haekwon Kim","raw_affiliation_strings":["Seoul Women's University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Seoul Women's University","institution_ids":["https://openalex.org/I98600543"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"36","issue":"1","first_page":"23","last_page":"34"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/umbilical-cord-derived-stem-cells","display_name":"umbilical cord-derived stem cells","score":0.9700000286102295},{"id":"https://openalex.org/keywords/mesenchymal-stem-cells","display_name":"mesenchymal stem cells","score":0.9810000061988831},{"id":"https://openalex.org/keywords/in-vitro-expansion","display_name":"in vitro expansion","score":0.9409999847412109},{"id":"https://openalex.org/keywords/stem-cell-differentiation","display_name":"stem cell differentiation","score":0.527999997138977},{"id":"https://openalex.org/keywords/ssea-3","display_name":"SSEA-3","score":0.6660000085830688},{"id":"https://openalex.org/keywords/ssea-4","display_name":"SSEA-4","score":1.0},{"id":"https://openalex.org/keywords/icam-1","display_name":"ICAM-1","score":0.8420000076293945},{"id":"https://openalex.org/keywords/hla-abc","display_name":"HLA-ABC","score":0.9549999833106995},{"id":"https://openalex.org/keywords/nestin","display_name":"nestin","score":0.8349999785423279},{"id":"https://openalex.org/keywords/vimentin","display_name":"vimentin","score":0.9869999885559082},{"id":"https://openalex.org/keywords/scf","display_name":"SCF","score":0.8429999947547913},{"id":"https://openalex.org/keywords/oct4","display_name":"Oct4","score":0.9359999895095825},{"id":"https://openalex.org/keywords/hnf4a","display_name":"HNF4\u03b1","score":0.890999972820282},{"id":"https://openalex.org/keywords/bmp4","display_name":"BMP4","score":0.6650000214576721},{"id":"https://openalex.org/keywords/fgf5","display_name":"FGF5","score":0.906000018119812},{"id":"https://openalex.org/keywords/cell-surface-markers","display_name":"cell surface markers","score":0.5740000009536743},{"id":"https://openalex.org/keywords/population-doubling-time","display_name":"population doubling time","score":0.671999990940094}],"concepts":[{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.7326725125312805},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5184870958328247},{"id":"https://openalex.org/C2776955114","wikidata":"https://www.wikidata.org/wiki/Q172563","display_name":"Umbilical cord","level":2,"score":0.499497652053833},{"id":"https://openalex.org/C86492073","wikidata":"https://www.wikidata.org/wiki/Q413766","display_name":"Fibronectin","level":3,"score":0.4985768795013428},{"id":"https://openalex.org/C198826908","wikidata":"https://www.wikidata.org/wiki/Q1922379","display_name":"Mesenchymal stem cell","level":2,"score":0.4780431389808655},{"id":"https://openalex.org/C35866371","wikidata":"https://www.wikidata.org/wiki/Q6005502","display_name":"Immunocytochemistry","level":2,"score":0.4586084485054016},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.4464586675167084},{"id":"https://openalex.org/C20875687","wikidata":"https://www.wikidata.org/wiki/Q2642600","display_name":"Adult stem cell","level":4,"score":0.4114408493041992},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.4107685089111328},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3961142599582672},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.3931577205657959},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.35489124059677124},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.353478342294693},{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.22317546606063843},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1984718143939972},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.1646486222743988},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.11258053779602051},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.05371639132499695},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2509814977","doi":null,"is_oa":false,"landing_page_url":"https://koreascience.or.kr/article/JAKO200921640757622.page","pdf_url":null,"source":{"id":"https://openalex.org/S2764373271","display_name":"Daehan saengsik uihak hoeji/Clinical and experimental reproductive medicine","issn_l":"2093-8896","issn":["2093-8896","2233-8233","2233-8241"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","kci-registered","poland-70","russia-white-list-2"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Daehan saengsik uihak hoeji/Clinical and experimental reproductive medicine","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8048999905586243}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W1539041175","https://openalex.org/W1767988311","https://openalex.org/W1804670053","https://openalex.org/W1928794896","https://openalex.org/W1965953554","https://openalex.org/W1969281312","https://openalex.org/W1969644104","https://openalex.org/W1972002957","https://openalex.org/W1976906682","https://openalex.org/W1980776838","https://openalex.org/W1987527399","https://openalex.org/W1991350431","https://openalex.org/W1996789930","https://openalex.org/W1998461585","https://openalex.org/W2005848913","https://openalex.org/W2009485059","https://openalex.org/W2012854380","https://openalex.org/W2013443778","https://openalex.org/W2014673628","https://openalex.org/W2019586013","https://openalex.org/W2035176325","https://openalex.org/W2036348189","https://openalex.org/W2038618794","https://openalex.org/W2045456080","https://openalex.org/W2049446122","https://openalex.org/W2050825851","https://openalex.org/W2082586271","https://openalex.org/W2092054789","https://openalex.org/W2092839502","https://openalex.org/W2103106557","https://openalex.org/W2104225093","https://openalex.org/W2107503723","https://openalex.org/W2128617483","https://openalex.org/W2141633761","https://openalex.org/W2142215404","https://openalex.org/W2163424015","https://openalex.org/W2170172182","https://openalex.org/W3143647952"],"related_works":["https://openalex.org/W1920138196","https://openalex.org/W3031937580","https://openalex.org/W2092054789","https://openalex.org/W2102643752","https://openalex.org/W4206240281","https://openalex.org/W2378094135","https://openalex.org/W4415130020","https://openalex.org/W2021141311","https://openalex.org/W2362790022","https://openalex.org/W4395115910"],"abstract_inverted_index":{"Objectives:":[0],"Mesenchymal":[1],"stem":[2,50,57,93,257],"cells":[3,58],"(MSC)":[4],"comprise":[5],"a":[6,30,36],"promising":[7],"tool":[8],"for":[9,77,214,271],"cellular":[10],"therapy.":[11],"It":[12],"is":[13],"known":[14],"that":[15,192,234,251],"long-term":[16],"in":[17,64,80,261],"vitro":[18,65,262],"culture":[19,131,148,163,181,222,266],"of":[20,32,38,43,53,92,109,122,141,152,169,212,255,265,274],"human":[21,54,72],"bone":[22],"marrow":[23],"and":[24,35,75,83,90,100,107,124,154,173,197,206,216,227,237],"adipose":[25],"tissue":[26],"derived-MSCs":[27],"lead":[28],"to":[29,47,112,177,241],"reduction":[31],"life":[33],"span":[34],"change":[37,113,253],"stem-like":[39],"characters.":[40],"The":[41],"aim":[42],"our":[44],"study":[45],"was":[46,157,186],"examine":[48],"whether":[49],"cell":[51,94,258],"properties":[52,95],"umbilical":[55,73],"cord-derived":[56],"(HUC)":[59],"could":[60],"be":[61,269],"affected":[62],"by":[63],"expansion.":[66],"Methods:":[67],"HUC":[68,110,193,213,252],"were":[69,88,96,200],"isolated":[70],"from":[71,115,133],"cord":[74],"cultured":[76],"10":[78],"passages":[79],"vitro.":[81],"Morphology":[82,106],"population":[84],"doubling":[85],"time":[86],"(PDT)":[87],"investigated,":[89],"changes":[91],"examined":[97],"using":[98],"RT-PCR":[99],"immunocytochemistry":[101],"during":[102,145,260],"serial":[103],"subcultures.":[104],"Results:":[105],"PDT":[108],"began":[111,240],"slightly":[114],"the":[116,130,146,162,180,184,221,272],"7th":[117],"passage":[118],"(p7).":[119],"Expression":[120,150,167],"level":[121,140,151,168],"nestin":[123],"vimentin":[125],"mRNAs":[126,156,175],"increased":[127,219,230],"along":[128],"with":[129,202],"period":[132,164],"p4":[134],"until":[135,165,245],"p10.":[136,166],"In":[137],"contrast,":[138],"expression":[139],"SCF":[142],"mRNA":[143],"decreased":[144],"same":[147],"period.":[149,223],"Oct-4":[153],"HNF-4\u03b1":[155],"not":[158,187],"significantly":[159],"changed":[160],"throughout":[161,220],"BMP-4,":[170],"FGF-5,":[171],"NCAM":[172],"HLA-ABC":[174,217],"appeared":[176],"increase":[178],"as":[179],"continued,":[182],"however,":[183],"difference":[185],"significant.":[188],"Immunocytochemical":[189],"studies":[190],"showed":[191],"at":[194,231,243],"p3,":[195],"p6":[196,244],"p9":[198,232],"positively":[199],"stained":[201],"antibodies":[203,229,239],"against":[204,225,235],"SSEA-3":[205],"SSEA-4":[207],"proteins.":[208],"Interestingly,":[209],"staining":[210],"intensity":[211],"ICAM-1":[215],"gradually":[218],"Intensity":[224],"thy-1":[226],"fibronectin":[228],"while":[233],"TRA-1-60":[236],"VCAM-1":[238],"decrease":[242],"p9.":[246],"Conclusions:":[247],"These":[248],"results":[249],"suggest":[250],"some":[254],"their":[256],"characteristics":[259],"culture.":[263],"Development":[264],"system":[267],"might":[268],"needed":[270],"maintenance":[273],"characteristics.":[275],"[Korean.":[276],"J.":[277],"Reprod.":[278],"Med.":[279],"2009;":[280],"36(1):":[281],"23-34.]":[282]},"counts_by_year":[{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-09-16T00:00:00"}