{"id":"https://openalex.org/W2501634603","doi":"https://doi.org/10.1021/bk-1995-0593.ch010","title":"Purification of the Human Immunodeficiency Virus-1 Aspartyl Proteinase Substrate Peptide","display_name":"Purification of the Human Immunodeficiency Virus-1 Aspartyl Proteinase Substrate Peptide","publication_year":1995,"publication_date":"1995-05-15","ids":{"openalex":"https://openalex.org/W2501634603","doi":"https://doi.org/10.1021/bk-1995-0593.ch010","mag":"2501634603"},"language":"en","primary_location":{"id":"doi:10.1021/bk-1995-0593.ch010","doi":"https://doi.org/10.1021/bk-1995-0593.ch010","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bk-1995-0593.ch010","pdf_url":null,"source":{"id":"https://openalex.org/S205053445","display_name":"ACS symposium series","issn_l":"0097-6156","issn":["0097-6156","1947-5918"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Symposium Series","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2483048766","display_name":"Modern Countercurrent Chromatography"},"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087354083","display_name":"Martha Knight","orcid":"https://orcid.org/0000-0003-4863-8858","observed_orcids":["https://orcid.org/0000-0003-4863-8858"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Martha Knight","raw_affiliation_strings":["Peptide Technologies Corporation, 8401 Helgerman Court, Gaithersburg, MD 20877"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Peptide Technologies Corporation, 8401 Helgerman Court, Gaithersburg, MD 20877","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029128268","display_name":"Yoichiro Ito","orcid":"https://orcid.org/0000-0001-9018-295X","observed_orcids":["https://orcid.org/0000-0001-9018-295X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoichiro Ito","raw_affiliation_strings":["Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 7N322, Bethesda, MD 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 7N322, Bethesda, MD 20892","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012349289","display_name":"Bhaskar Chandrasekhar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bhaskar Chandrasekhar","raw_affiliation_strings":["Peptide Technologies Corporation, 8401 Helgerman Court, Gaithersburg, MD 20877"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Peptide Technologies Corporation, 8401 Helgerman Court, Gaithersburg, MD 20877","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Kazuyuki Takahashi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kazuyuki Takahashi","raw_affiliation_strings":["Peptide Technologies Corporation, 8401 Helgerman Court, Gaithersburg, MD 20877"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Peptide Technologies Corporation, 8401 Helgerman Court, Gaithersburg, MD 20877","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009025004","display_name":"Anil B. Mukherjee","orcid":"https://orcid.org/0000-0003-4445-5464","observed_orcids":["https://orcid.org/0000-0003-4445-5464"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anil B. Mukherjee","raw_affiliation_strings":["Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.8908,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.88839286,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"111","last_page":"118"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.7192000150680542,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.7192000150680542,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.05790000036358833,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.04670000076293945,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/radiolabeled-substrates","display_name":"radiolabeled substrates","score":0.7289999723434448},{"id":"https://openalex.org/keywords/peptide-purification","display_name":"peptide purification","score":0.6499999761581421},{"id":"https://openalex.org/keywords/activated-charcoal-adsorption","display_name":"charcoal adsorption","score":0.7919999957084656}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7484760284423828},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.722876787185669},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.6821835041046143},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.6713045835494995},{"id":"https://openalex.org/C2780112151","wikidata":"https://www.wikidata.org/wiki/Q412033","display_name":"Trifluoroacetic acid","level":2,"score":0.5810056924819946},{"id":"https://openalex.org/C73826308","wikidata":"https://www.wikidata.org/wiki/Q684907","display_name":"Elution","level":2,"score":0.5500916838645935},{"id":"https://openalex.org/C62538594","wikidata":"https://www.wikidata.org/wiki/Q54389","display_name":"Tritium","level":2,"score":0.4793618321418762},{"id":"https://openalex.org/C2780471494","wikidata":"https://www.wikidata.org/wiki/Q146505","display_name":"Solvent","level":2,"score":0.4778832495212555},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.4281138479709625},{"id":"https://openalex.org/C2777774944","wikidata":"https://www.wikidata.org/wiki/Q417262","display_name":"Endopeptidase","level":3,"score":0.42481881380081177},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.25331151485443115},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2459087073802948},{"id":"https://openalex.org/C111368507","wikidata":"https://www.wikidata.org/wiki/Q43518","display_name":"Oceanography","level":1,"score":0.0},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1021/bk-1995-0593.ch010","doi":"https://doi.org/10.1021/bk-1995-0593.ch010","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bk-1995-0593.ch010","pdf_url":null,"source":{"id":"https://openalex.org/S205053445","display_name":"ACS symposium series","issn_l":"0097-6156","issn":["0097-6156","1947-5918"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Symposium Series","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320309030","display_name":"Small Business Innovation Research","ror":"https://ror.org/015t55b95"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2044422624","https://openalex.org/W2020784172","https://openalex.org/W2018896510","https://openalex.org/W2093439024","https://openalex.org/W2019865129","https://openalex.org/W2095300338","https://openalex.org/W2316906870","https://openalex.org/W2028275712","https://openalex.org/W2417989207","https://openalex.org/W2141426282"],"abstract_inverted_index":{"A":[0,21],"rapid":[1,78],"and":[2,28,100,108,116,145],"sensitive":[3],"assay":[4],"for":[5,17],"the":[6,11,26,30,32,40,52,67,71,93,131,136,151],"aspartyl":[7],"protease":[8],"enzyme":[9],"of":[10,113,120],"HIV-1":[12],"virus":[13],"is":[14,35,42],"being":[15],"developed":[16],"detecting":[18],"potential":[19],"inhibitors.":[20],"tritiated":[22],"peptide":[23,74,139],"serves":[24],"as":[25,54],"substrate":[27,41,59,73],"after":[29,130],"reaction,":[31],"radiolabeled":[33,72],"product":[34],"counted":[36],"in":[37,66,92,150],"solution":[38,45],"while":[39],"removed":[43],"from":[44],"by":[46,77,106,135],"adsorption":[47],"onto":[48],"charcoal":[49],"added":[50],"to":[51],"tube":[53],"a":[55,87,111,117],"tablet.":[56],"The":[57,81,123,138],"labeled":[58],"was":[60,75,84,140],"synthesized":[61],"with":[62],"an":[63],"unsaturated":[64],"proline":[65],"sequence.":[68],"After":[69],"tritiation":[70,82],"isolated":[76],"countercurrent":[79],"chromatography.":[80],"reaction":[83],"loaded":[85],"on":[86],"multi-layer":[88],"coil":[89],"planet":[90],"centrifuge":[91],"solvent":[94,132],"system":[95],"t-butyl":[96],"methyl":[97],"ether,":[98],"acetonitrile":[99],"1%":[101],"aq.":[102],"trifluoroacetic":[103],"acid":[104],"(2:2:3":[105],"volume)":[107],"chromatographed":[109],"at":[110],"flow":[112],"120":[114],"ml/hr":[115],"centrifugation":[118],"rate":[119],"800":[121],"rpm.":[122],"excess":[124],"radioactive":[125],"side":[126],"products":[127],"were":[128],"eluted":[129],"front":[133],"followed":[134],"peptide.":[137],"separated":[141],"within":[142],"1":[143],"hr":[144],"could":[146],"be":[147],"directly":[148],"used":[149],"experiments.":[152]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-08-23T00:00:00"}
