{"id":"https://openalex.org/W2495779946","doi":"https://doi.org/10.1385/1-59259-177-9:197","title":"PCR-Mediated Recombination: A General Method Applied to Construct Chimeric Infectious Molecular Clones","display_name":"PCR-Mediated Recombination: A General Method Applied to Construct Chimeric Infectious Molecular Clones","publication_year":2003,"publication_date":"2003-11-14","ids":{"openalex":"https://openalex.org/W2495779946","doi":"https://doi.org/10.1385/1-59259-177-9:197","mag":"2495779946"},"language":"en","primary_location":{"id":"doi:10.1385/1-59259-177-9:197","doi":"https://doi.org/10.1385/1-59259-177-9:197","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-177-9:197","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PCR Cloning Protocols","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W1543710993","display_name":"PCR Cloning Protocols"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Guowei Fang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Guowei Fang","raw_affiliation_strings":["Wadsworth Center, Albany, NY","Wadsworth Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Albany, NY","institution_ids":["https://openalex.org/I1339417872"]},{"raw_affiliation_string":"Wadsworth Center","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058508912","display_name":"Barbara Weiser","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Barbara Weiser","raw_affiliation_strings":["Wadsworth Center, Albany, NY","Wadsworth Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Albany, NY","institution_ids":["https://openalex.org/I1339417872"]},{"raw_affiliation_string":"Wadsworth Center","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051091614","display_name":"Aloise Visosky","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aloise Visosky","raw_affiliation_strings":["Wadsworth Center, Albany, NY","Wadsworth Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Albany, NY","institution_ids":["https://openalex.org/I1339417872"]},{"raw_affiliation_string":"Wadsworth Center","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058772320","display_name":"Timothy H. Moran","orcid":"https://orcid.org/0000-0001-9877-8723","observed_orcids":["https://orcid.org/0000-0001-9877-8723"]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Timothy Moran","raw_affiliation_strings":["Wadsworth Center, Albany, NY","Wadsworth Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Albany, NY","institution_ids":["https://openalex.org/I1339417872"]},{"raw_affiliation_string":"Wadsworth Center","institution_ids":["https://openalex.org/I1339417872"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019683154","display_name":"Harold Burger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1339417872","display_name":"Wadsworth Center","ror":"https://ror.org/050kf9c55","country_code":"US","type":"facility","lineage":["https://openalex.org/I1298060332","https://openalex.org/I1339417872"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Harold Burger","raw_affiliation_strings":["Wadsworth Center, Albany, NY","Wadsworth Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wadsworth Center, Albany, NY","institution_ids":["https://openalex.org/I1339417872"]},{"raw_affiliation_string":"Wadsworth Center","institution_ids":["https://openalex.org/I1339417872"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1339417872"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.32339047,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":93},"biblio":{"volume":null,"issue":null,"first_page":"197","last_page":"206"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.024299999698996544,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.009499999694526196,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombinant-dna-construction","display_name":"recombinant DNA construction","score":0.6510000228881836},{"id":"https://openalex.org/keywords/vaccine-development","display_name":"vaccine development","score":0.640999972820282},{"id":"https://openalex.org/keywords/microbial-pathogenesis","display_name":"microbial pathogenesis","score":0.9810000061988831}],"concepts":[{"id":"https://openalex.org/C203284983","wikidata":"https://www.wikidata.org/wiki/Q17087215","display_name":"In vitro recombination","level":5,"score":0.6849744319915771},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6001077890396118},{"id":"https://openalex.org/C58301748","wikidata":"https://www.wikidata.org/wiki/Q1074969","display_name":"Restriction site","level":4,"score":0.5762646198272705},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5733987092971802},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5394346117973328},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.5159373879432678},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.511258602142334},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4952475130558014},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.487535685300827},{"id":"https://openalex.org/C179437574","wikidata":"https://www.wikidata.org/wiki/Q15089798","display_name":"Ligation","level":2,"score":0.47963958978652954},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.4583300054073334},{"id":"https://openalex.org/C52182516","wikidata":"https://www.wikidata.org/wiki/Q17144128","display_name":"Sequencing by ligation","level":5,"score":0.43222346901893616},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.4225800633430481},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.41247838735580444},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.39481741189956665},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2378980815410614},{"id":"https://openalex.org/C13575015","wikidata":"https://www.wikidata.org/wiki/Q1562492","display_name":"Genomic library","level":4,"score":0.18244829773902893},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.14729925990104675},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.1281035840511322},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.10643789172172546},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1385/1-59259-177-9:197","doi":"https://doi.org/10.1385/1-59259-177-9:197","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-177-9:197","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PCR Cloning Protocols","raw_type":"book-chapter"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1507665550","https://openalex.org/W1546157935","https://openalex.org/W1988837048","https://openalex.org/W1991509441","https://openalex.org/W2006762876","https://openalex.org/W2007305161","https://openalex.org/W2018488189","https://openalex.org/W2024564432","https://openalex.org/W2030844138","https://openalex.org/W2032118018","https://openalex.org/W2070358105","https://openalex.org/W2075574711","https://openalex.org/W2090081965","https://openalex.org/W2122139577","https://openalex.org/W2145311627","https://openalex.org/W2170219825","https://openalex.org/W2331366558"],"related_works":["https://openalex.org/W1507665550","https://openalex.org/W59365859","https://openalex.org/W2256396417","https://openalex.org/W2410799347","https://openalex.org/W2018488189","https://openalex.org/W2417837866","https://openalex.org/W2056268178","https://openalex.org/W1970650210","https://openalex.org/W1964166382","https://openalex.org/W1604670117"],"abstract_inverted_index":{"Molecular":[0],"cloning":[1,38],"has":[2,113],"proven":[3],"to":[4,61,116,134],"be":[5,31,89,182],"a":[6,17,121],"powerful":[7],"tool":[8],"in":[9,16,68,78],"biology,":[10],"and":[11,24,43,143,169,178],"chimeric":[12],"clones":[13],"are":[14,103,131,148],"useful":[15],"variety":[18],"of":[19,27,58,64,75,81,120],"fields":[20],"including":[21],"microbial":[22],"pathogenesis":[23],"the":[25,56,69,73,82,155,172,179,185],"development":[26],"vaccines.":[28],"Chimeras":[29],"can":[30],"created":[32,90],"from":[33,55],"DNA":[34,44,86,101,118],"by":[35,150,167,171],"using":[36],"conventional":[37],"techniques,":[39,47],"specifically":[40],"restriction":[41,59,76],"cleavage":[42],"ligation.":[45],"Such":[46],"however,":[48],"have":[49],"limitations;":[50],"most":[51],"commonly,":[52],"limitations":[53],"result":[54],"lack":[57],"sites":[60,77],"provide":[62],"points":[63],"entry":[65],"for":[66],"inserts":[67],"desired":[70],"regions":[71,80],"or":[72,98],"multiplicity":[74],"other":[79],"DNA.":[83],"Because":[84],"recombinant":[85],"molecules":[87],"may":[88,181],"during":[91,154],"polymerase":[92],"chain":[93],"reaction":[94,157],"(PCR)":[95],"when":[96],"two":[97,132],"more":[99],"different":[100],"sequences":[102],"brought":[104],"together":[105],"(":[106,125,158],"1":[107],",":[108,160],"2":[109],"),":[110],"PCR-mediated":[111],"recombination":[112],"been":[114],"exploited":[115],"join":[117],"fragments":[119],"few":[122],"hundred":[123],"bases":[124],"3":[126],"-":[127],"8":[128],").":[129,162],"There":[130],"drawbacks":[133],"these":[135],"methods.":[136],"First,":[137],"they":[138],"often":[139],"involve":[140],"multiple":[141],"steps,":[142],"second,":[144],"sequence":[145],"errors":[146],"frequently":[147],"introduced":[149],"certain":[151],"thermostable":[152],"polymerases":[153],"PCR":[156],"9":[159],"10":[161],"These":[163],"keywords":[164,180],"were":[165],"added":[166],"machine":[168],"not":[170],"authors.":[173],"This":[174],"process":[175],"is":[176],"experimental":[177],"updated":[183],"as":[184],"learning":[186],"algorithm":[187],"improves.":[188]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-08-23T00:00:00"}