{"id":"https://openalex.org/W2492446723","doi":"https://doi.org/10.1002/rcm.7577","title":"Identification of 4\u2010hydroxynonenal protein targets in rat, mouse and human liver microsomes by two\u2010dimensional liquid chromatography/tandem mass spectrometry","display_name":"Identification of 4\u2010hydroxynonenal protein targets in rat, mouse and human liver microsomes by two\u2010dimensional liquid chromatography/tandem mass spectrometry","publication_year":2016,"publication_date":"2016-06-07","ids":{"openalex":"https://openalex.org/W2492446723","doi":"https://doi.org/10.1002/rcm.7577","mag":"2492446723","pmid":"https://pubmed.ncbi.nlm.nih.gov/27321836"},"language":"en","primary_location":{"id":"doi:10.1002/rcm.7577","doi":"https://doi.org/10.1002/rcm.7577","is_oa":false,"landing_page_url":"https://doi.org/10.1002/rcm.7577","pdf_url":null,"source":{"id":"https://openalex.org/S89689683","display_name":"Rapid Communications in Mass Spectrometry","issn_l":"0951-4198","issn":["0951-4198","1097-0231"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid Communications in Mass Spectrometry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087950841","display_name":"Makan Golizeh","orcid":"https://orcid.org/0000-0001-7746-5533","observed_orcids":["https://orcid.org/0000-0001-7746-5533"]},"institutions":[{"id":"https://openalex.org/I159129438","display_name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al","ror":"https://ror.org/002rjbv21","country_code":"CA","type":"education","lineage":["https://openalex.org/I159129438","https://openalex.org/I49663120"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Makan Golizeh","raw_affiliation_strings":["Chemistry Department/Pharmaqam Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al (UQ\u00c0M)  Montr\u00e9al QC Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department/Pharmaqam Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al (UQ\u00c0M)  Montr\u00e9al QC Canada","institution_ids":["https://openalex.org/I159129438"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043897692","display_name":"Timon Geib","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I159129438","display_name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al","ror":"https://ror.org/002rjbv21","country_code":"CA","type":"education","lineage":["https://openalex.org/I159129438","https://openalex.org/I49663120"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Timon Geib","raw_affiliation_strings":["Chemistry Department/Pharmaqam Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al (UQ\u00c0M)  Montr\u00e9al QC Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department/Pharmaqam Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al (UQ\u00c0M)  Montr\u00e9al QC Canada","institution_ids":["https://openalex.org/I159129438"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088289614","display_name":"Lekha Sleno","orcid":"https://orcid.org/0000-0001-7923-8019","observed_orcids":["https://orcid.org/0000-0001-7923-8019"]},"institutions":[{"id":"https://openalex.org/I159129438","display_name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al","ror":"https://ror.org/002rjbv21","country_code":"CA","type":"education","lineage":["https://openalex.org/I159129438","https://openalex.org/I49663120"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Lekha Sleno","raw_affiliation_strings":["Chemistry Department/Pharmaqam Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al (UQ\u00c0M)  Montr\u00e9al QC Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department/Pharmaqam Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al (UQ\u00c0M)  Montr\u00e9al QC Canada","institution_ids":["https://openalex.org/I159129438"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5088289614"],"corresponding_institution_ids":["https://openalex.org/I159129438"],"apc_list":{"value":4840,"currency":"USD","value_usd":4840},"apc_paid":null,"fwci":0.452,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.67486611,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"30","issue":"13","first_page":"1488","last_page":"1494"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.2378000020980835,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.03849999979138374,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/4-hydroxynonenal","display_name":"4-hydroxynonenal","score":1.0},{"id":"https://openalex.org/keywords/protein-carbonylation","display_name":"protein carbonylation","score":0.7829999923706055},{"id":"https://openalex.org/keywords/liver-microsomes","display_name":"liver microsomes","score":0.9020000100135803},{"id":"https://openalex.org/keywords/lipid-peroxidation","display_name":"lipid peroxidation","score":0.7179999947547913},{"id":"https://openalex.org/keywords/reactive-metabolites","display_name":"reactive metabolites","score":0.5770000219345093},{"id":"https://openalex.org/keywords/atp-synthase","display_name":"ATP synthase","score":0.8190000057220459},{"id":"https://openalex.org/keywords/cyp1a2","display_name":"CYP1A2","score":0.9700000286102295},{"id":"https://openalex.org/keywords/protein-disulfide-isomerase","display_name":"protein disulfide isomerase","score":0.875},{"id":"https://openalex.org/keywords/ergic-53","display_name":"ERGIC-53","score":0.8840000033378601},{"id":"https://openalex.org/keywords/glud1","display_name":"GLUD1","score":0.9480000138282776},{"id":"https://openalex.org/keywords/carbamoyl-phosphate-synthase","display_name":"carbamoyl-phosphate synthase","score":0.9950000047683716},{"id":"https://openalex.org/keywords/cross-species-comparison","display_name":"cross-species comparison","score":0.6600000262260437}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8995038270950317},{"id":"https://openalex.org/C87644729","wikidata":"https://www.wikidata.org/wiki/Q547502","display_name":"Microsome","level":3,"score":0.6432894468307495},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6274707317352295},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.5678572654724121},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.4974842369556427},{"id":"https://openalex.org/C5098756","wikidata":"https://www.wikidata.org/wiki/Q1641611","display_name":"Protein disulfide-isomerase","level":3,"score":0.4774359166622162},{"id":"https://openalex.org/C40684141","wikidata":"https://www.wikidata.org/wiki/Q902061","display_name":"Electrospray ionization","level":3,"score":0.47248902916908264},{"id":"https://openalex.org/C2779308327","wikidata":"https://www.wikidata.org/wiki/Q229982","display_name":"4-Hydroxynonenal","level":4,"score":0.4568522572517395},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.45373550057411194},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.45108988881111145},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41093748807907104},{"id":"https://openalex.org/C2780829032","wikidata":"https://www.wikidata.org/wiki/Q898539","display_name":"Lipid peroxidation","level":3,"score":0.338600218296051},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2275075614452362},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000447","descriptor_name":"Aldehydes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000447","descriptor_name":"Aldehydes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000447","descriptor_name":"Aldehydes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008862","descriptor_name":"Microsomes, Liver","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008862","descriptor_name":"Microsomes, Liver","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008862","descriptor_name":"Microsomes, Liver","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/rcm.7577","doi":"https://doi.org/10.1002/rcm.7577","is_oa":false,"landing_page_url":"https://doi.org/10.1002/rcm.7577","pdf_url":null,"source":{"id":"https://openalex.org/S89689683","display_name":"Rapid Communications in Mass Spectrometry","issn_l":"0951-4198","issn":["0951-4198","1097-0231"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid Communications in Mass Spectrometry","raw_type":"journal-article"},{"id":"pmid:27321836","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27321836","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rapid communications in mass spectrometry : RCM","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":62,"referenced_works":["https://openalex.org/W160522190","https://openalex.org/W176790139","https://openalex.org/W1493260517","https://openalex.org/W1502736133","https://openalex.org/W1597678601","https://openalex.org/W1967103312","https://openalex.org/W1968515763","https://openalex.org/W1972011132","https://openalex.org/W1975488757","https://openalex.org/W1976300978","https://openalex.org/W1978104803","https://openalex.org/W1983905590","https://openalex.org/W1986363766","https://openalex.org/W1989822993","https://openalex.org/W1993457485","https://openalex.org/W1998316952","https://openalex.org/W2001296325","https://openalex.org/W2008952528","https://openalex.org/W2019950093","https://openalex.org/W2021313784","https://openalex.org/W2033851723","https://openalex.org/W2036394515","https://openalex.org/W2038493537","https://openalex.org/W2043445707","https://openalex.org/W2044357091","https://openalex.org/W2051386224","https://openalex.org/W2055460924","https://openalex.org/W2056897039","https://openalex.org/W2057522312","https://openalex.org/W2057649106","https://openalex.org/W2058927664","https://openalex.org/W2063334273","https://openalex.org/W2068980704","https://openalex.org/W2070857907","https://openalex.org/W2071537307","https://openalex.org/W2071732607","https://openalex.org/W2072471260","https://openalex.org/W2073224154","https://openalex.org/W2073959755","https://openalex.org/W2081695107","https://openalex.org/W2082514969","https://openalex.org/W2088357907","https://openalex.org/W2091643856","https://openalex.org/W2094619254","https://openalex.org/W2095849274","https://openalex.org/W2098472712","https://openalex.org/W2105619026","https://openalex.org/W2110966376","https://openalex.org/W2115129497","https://openalex.org/W2120866788","https://openalex.org/W2129141040","https://openalex.org/W2133845492","https://openalex.org/W2134087185","https://openalex.org/W2135590784","https://openalex.org/W2136222747","https://openalex.org/W2142755889","https://openalex.org/W2144050249","https://openalex.org/W2153796162","https://openalex.org/W2154534038","https://openalex.org/W2155382386","https://openalex.org/W2161574105","https://openalex.org/W2321973970"],"related_works":["https://openalex.org/W1991358375","https://openalex.org/W2003299808","https://openalex.org/W1988661674","https://openalex.org/W964154261","https://openalex.org/W1999397561","https://openalex.org/W2091473231","https://openalex.org/W2000133861","https://openalex.org/W2044016383","https://openalex.org/W2020902296","https://openalex.org/W1969076756"],"abstract_inverted_index":{"RATIONALE:":[0],"4-Hydroxynonenal":[1],"(HNE),":[2],"endogenously":[3],"generated":[4],"through":[5,23],"peroxidation":[6],"and":[7,38,58,70,80,113,118,121,127,137,146,154,188,202,211,233,254],"breakdown":[8],"of":[9,19,25,30,134,224,250,262],"polyunsaturated":[10],"fatty":[11],"acids,":[12],"has":[13],"been":[14],"linked":[15],"to":[16,32,82,88,96,151,163,213,246],"a":[17,52],"number":[18],"adverse":[20,46],"biological":[21],"effects":[22],"carbonylation":[24],"essential":[26],"biomolecules.":[27],"Covalent":[28],"binding":[29],"HNE":[31,167,227,248,263],"proteins":[33,60,160,195,251],"can":[34,243],"alter":[35,208],"their":[36,203,209],"structure":[37],"functions,":[39],"causing":[40],"cell":[41],"damage":[42],"as":[43,45],"well":[44],"immune":[47],"responses.":[48],"The":[49],"liver":[50,72,148,235],"plays":[51],"predominant":[53],"role":[54],"in":[55,143,168,199,225,230,252,255],"metabolic":[56],"transformations":[57],"hepatic":[59],"are":[61],"often":[62],"targeted":[63],"by":[64,108,166],"reactive":[65],"metabolites.":[66],"METHODS:":[67],"Rat,":[68],"mouse":[69,145,232],"human":[71,147,234],"microsomes":[73,149,236],"were":[74,106,141,161],"incubated":[75],"with":[76],"HNE,":[77],"enzymatically":[78],"digested,":[79],"subjected":[81],"strong":[83],"cation-exchange":[84],"peptide":[85,110],"fractionation":[86],"prior":[87],"liquid":[89],"chromatography/tandem":[90],"mass":[91,102,126],"spectrometry":[92],"(LC/MS/MS)":[93],"analysis":[94,115],"coupled":[95],"electrospray":[97],"ionization":[98],"quadrupole":[99],"time-of-flight":[100],"(QqTOF)":[101],"spectrometry.":[103],"HNE-modified":[104,139],"peptides":[105,140],"detected":[107],"probability-driven":[109],"spectral":[111],"matching":[112],"comparative":[114],"between":[116],"treated":[117],"control":[119],"samples,":[120],"confirmed":[122],"based":[123],"on":[124],"accurate":[125],"high-resolution":[128],"MS/MS":[129],"spectra.":[130],"RESULTS:":[131],"A":[132],"total":[133],"99,":[135],"123":[136],"51":[138],"identified":[142],"rat,":[144,231],"related":[150],"76,":[152],"103":[153],"44":[155],"target":[156],"proteins,":[157],"respectively.":[158],"Eight":[159],"found":[162],"be":[164,244],"adducted":[165],"all":[169],"three":[170],"species,":[171],"including":[172],"ATP":[173],"synthase,":[174,177],"carbamoyl":[175],"phosphate":[176],"cytochrome":[178],"P450":[179],"1A2,":[180],"glutamate":[181],"dehydrogenase":[182],"1,":[183],"protein":[184,186,192,228,264],"ERGIC-53,":[185],"disulfide-isomerase,":[187],"voltage-dependent":[189],"anion-selective":[190],"channel":[191],"1.":[193],"These":[194],"play":[196],"crucial":[197],"roles":[198],"cellular":[200],"processes":[201],"covalent":[204],"modification":[205,249],"could":[206],"potentially":[207],"function":[210],"lead":[212],"cytotoxicity.":[214],"CONCLUSIONS:":[215],"An":[216],"analytical":[217],"approach":[218,242],"was":[219],"developed":[220],"for":[221],"the":[222,260],"identification":[223],"vitro":[226,253],"targets":[229],"using":[237],"two-dimensional":[238],"(2D)":[239],"LC/MS/MS.":[240],"This":[241],"applied":[245],"study":[247],"vivo,":[256],"providing":[257],"insight":[258],"into":[259],"toxicology":[261],"adduction.":[265],"Copyright":[266],"\u00a9":[267],"2016":[268],"John":[269],"Wiley":[270],"&":[271],"Sons,":[272],"Ltd.":[273]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":5},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2025-10-10T00:00:00"}