{"id":"https://openalex.org/W2491618243","doi":"https://doi.org/10.1002/jcp.25492","title":"Extracellular Proteasomes Are Deficient in 19S Subunits as Revealed by iTRAQ Quantitative Proteomics","display_name":"Extracellular Proteasomes Are Deficient in 19S Subunits as Revealed by iTRAQ Quantitative Proteomics","publication_year":2016,"publication_date":"2016-07-19","ids":{"openalex":"https://openalex.org/W2491618243","doi":"https://doi.org/10.1002/jcp.25492","mag":"2491618243","pmid":"https://pubmed.ncbi.nlm.nih.gov/27430664"},"language":"en","primary_location":{"id":"doi:10.1002/jcp.25492","doi":"https://doi.org/10.1002/jcp.25492","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jcp.25492","pdf_url":null,"source":{"id":"https://openalex.org/S43643205","display_name":"Journal of Cellular Physiology","issn_l":"0021-9541","issn":["0021-9541","1097-4652"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cellular Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076067088","display_name":"\u0410. \u0421. \u0426\u0438\u043c\u043e\u0445\u0430","orcid":"https://orcid.org/0000-0002-8261-2750","observed_orcids":["https://orcid.org/0000-0002-8261-2750"]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I4210104940","display_name":"Institute of Cytology","ror":"https://ror.org/01p3q4q56","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210104940","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Anna S. Tsimokha","raw_affiliation_strings":["Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210104940"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037961842","display_name":"Julia J. Zaykova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I4210104940","display_name":"Institute of Cytology","ror":"https://ror.org/01p3q4q56","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210104940","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Julia J. Zaykova","raw_affiliation_strings":["Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210104940"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007350471","display_name":"Andrew R. Bottrill","orcid":"https://orcid.org/0000-0002-5182-3643","observed_orcids":["https://orcid.org/0000-0002-5182-3643"]},"institutions":[{"id":"https://openalex.org/I153648349","display_name":"University of Leicester","ror":"https://ror.org/04h699437","country_code":"GB","type":"education","lineage":["https://openalex.org/I153648349"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Andrew Bottrill","raw_affiliation_strings":["Department of Biochemistry, University of Leicester, Leicester, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Leicester, Leicester, United Kingdom","institution_ids":["https://openalex.org/I153648349"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037735971","display_name":"Nickolai A. Barlev","orcid":"https://orcid.org/0000-0001-7111-2446","observed_orcids":["https://orcid.org/0000-0001-7111-2446"]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I4210104940","display_name":"Institute of Cytology","ror":"https://ror.org/01p3q4q56","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210104940","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Nikolai A. Barlev","raw_affiliation_strings":["Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210104940"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5037735971","https://openalex.org/A5076067088"],"corresponding_institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210104940"],"apc_list":{"value":5230,"currency":"USD","value_usd":5230},"apc_paid":null,"fwci":1.1523,"has_fulltext":false,"cited_by_count":24,"citation_normalized_percentile":{"value":0.77135756,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":98},"biblio":{"volume":"232","issue":"4","first_page":"842","last_page":"851"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/19s-regulatory-particle","display_name":"19S regulatory particle","score":0.875},{"id":"https://openalex.org/keywords/20s-proteasome","display_name":"20S proteasome","score":0.9169999957084656},{"id":"https://openalex.org/keywords/proteasome-subunit-composition","display_name":"proteasome subunit composition","score":0.8970000147819519},{"id":"https://openalex.org/keywords/k562-cells","display_name":"K562 cells","score":0.8069999814033508},{"id":"https://openalex.org/keywords/conditioned-medium","display_name":"conditioned medium","score":0.7950000166893005},{"id":"https://openalex.org/keywords/extracellular-proteolysis","display_name":"extracellular proteolysis","score":0.7009999752044678},{"id":"https://openalex.org/keywords/ubiquitin-system","display_name":"ubiquitin system","score":0.7350000143051147},{"id":"https://openalex.org/keywords/protein-synthesis","display_name":"protein synthesis","score":0.675000011920929}],"concepts":[{"id":"https://openalex.org/C27740335","wikidata":"https://www.wikidata.org/wiki/Q14878333","display_name":"Proteasome","level":2,"score":0.956729531288147},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.8487787842750549},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.6479943990707397},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.6093829870223999},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.5871730446815491},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.5560876727104187},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.5391483902931213},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.5353204607963562},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5183552503585815},{"id":"https://openalex.org/C25602115","wikidata":"https://www.wikidata.org/wiki/Q407111","display_name":"Ubiquitin","level":3,"score":0.503426730632782},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.486039936542511},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4666580855846405},{"id":"https://openalex.org/C80311884","wikidata":"https://www.wikidata.org/wiki/Q7268939","display_name":"Quantitative proteomics","level":4,"score":0.41550254821777344},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.407998263835907},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1387149691581726},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0989392101764679}],"mesh":[{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D017077","descriptor_name":"Culture Media, Conditioned","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D020014","descriptor_name":"K562 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D046988","descriptor_name":"Proteasome Endopeptidase Complex","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/jcp.25492","doi":"https://doi.org/10.1002/jcp.25492","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jcp.25492","pdf_url":null,"source":{"id":"https://openalex.org/S43643205","display_name":"Journal of Cellular Physiology","issn_l":"0021-9541","issn":["0021-9541","1097-4652"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cellular Physiology","raw_type":"journal-article"},{"id":"pmid:27430664","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27430664","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cellular physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1770913197","display_name":null,"funder_award_id":"\u211614-50-00068","funder_id":"https://openalex.org/F4320324099","funder_display_name":"Russian Science Foundation"},{"id":"https://openalex.org/G4632086512","display_name":null,"funder_award_id":"15-04-08128","funder_id":"https://openalex.org/F4320321079","funder_display_name":"Russian Foundation for Basic Research"}],"funders":[{"id":"https://openalex.org/F4320321079","display_name":"Russian Foundation for Basic Research","ror":"https://ror.org/02mh1ke95"},{"id":"https://openalex.org/F4320324099","display_name":"Russian Science Foundation","ror":"https://ror.org/03y2gwe85"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":57,"referenced_works":["https://openalex.org/W1536763098","https://openalex.org/W1555023450","https://openalex.org/W1556426431","https://openalex.org/W1839188703","https://openalex.org/W1892376069","https://openalex.org/W1918838535","https://openalex.org/W1968427762","https://openalex.org/W1971608293","https://openalex.org/W1978542424","https://openalex.org/W1981593008","https://openalex.org/W1985591441","https://openalex.org/W1988448896","https://openalex.org/W1988746802","https://openalex.org/W1991856249","https://openalex.org/W1994109599","https://openalex.org/W1999119754","https://openalex.org/W1999330819","https://openalex.org/W2001265993","https://openalex.org/W2013236411","https://openalex.org/W2015556811","https://openalex.org/W2017247080","https://openalex.org/W2018228226","https://openalex.org/W2021242983","https://openalex.org/W2024627774","https://openalex.org/W2025422229","https://openalex.org/W2036460912","https://openalex.org/W2037913550","https://openalex.org/W2040030781","https://openalex.org/W2044085158","https://openalex.org/W2052245332","https://openalex.org/W2059694525","https://openalex.org/W2059745268","https://openalex.org/W2059963644","https://openalex.org/W2061632343","https://openalex.org/W2070367241","https://openalex.org/W2074840619","https://openalex.org/W2082460299","https://openalex.org/W2089130084","https://openalex.org/W2090021429","https://openalex.org/W2097164847","https://openalex.org/W2100573143","https://openalex.org/W2112078820","https://openalex.org/W2123030744","https://openalex.org/W2123781000","https://openalex.org/W2130706354","https://openalex.org/W2149060516","https://openalex.org/W2150123812","https://openalex.org/W2158217645","https://openalex.org/W2159887483","https://openalex.org/W2166987747","https://openalex.org/W2209848665","https://openalex.org/W2332230112","https://openalex.org/W2407251337","https://openalex.org/W2416592785","https://openalex.org/W2435186963","https://openalex.org/W2464602036","https://openalex.org/W4211208250"],"related_works":["https://openalex.org/W1978830294","https://openalex.org/W4224285838","https://openalex.org/W2767132232","https://openalex.org/W2410258297","https://openalex.org/W3046310606","https://openalex.org/W1573531091","https://openalex.org/W7096782215","https://openalex.org/W2903327908","https://openalex.org/W2052245332","https://openalex.org/W1982107660"],"abstract_inverted_index":{"Proteasome-mediated":[0],"proteolysis":[1],"is":[2],"critical":[3],"for":[4,207],"regulation":[5],"of":[6,9,35,75,92,96,105,113,127,154,171,173,190],"vast":[7],"majority":[8],"cellular":[10,59],"processes.":[11],"In":[12],"addition":[13],"to":[14,87,122,145,203],"their":[15,64],"well-documented":[16],"functions":[17,34],"in":[18,28,135,196,224,235],"the":[19,50,106,114,123,174,188,191,197,204,208,212,225,230],"nucleus":[20],"and":[21,33,52,58,90,109,119,177],"cytoplasm":[22],"proteasomes":[23,60,199,232],"have":[24,243],"also":[25],"been":[26],"found":[27],"extracellular":[29,42,56,198,231],"space.":[30],"The":[31,147],"origin":[32],"these":[36],"proteasomes,":[37,43],"dubbed":[38],"as":[39],"circulating/plasmatic":[40],"or":[41,80],"are":[44,233],"unclear.":[45],"To":[46],"gain":[47],"insights":[48],"into":[49],"molecular":[51],"functional":[53],"differences":[54],"between":[55],"(EPs)":[57],"(CPs)":[61],"we":[62,185],"compared":[63,202],"subunit":[65],"composition":[66],"using":[67],"iTRAQ-based":[68],"quantitative":[69],"proteomics":[70],"(iTRAQ":[71],"LC/MS-MS).":[72],"Our":[73],"analysis":[74,149],"purified":[76],"from":[77,143],"K562":[78],"cells":[79],"conditioned":[81],"medium":[82],"intact":[83],"proteasome":[84,101,108,128,175],"complexes":[85,195],"led":[86],"an":[88],"identification":[89],"quantification":[91],"114":[93],"proteins,":[94],"out":[95],"which":[97,238],"19":[98],"were":[99,132,156,164,179],"26S":[100],"proteins":[102,130,176],"(all":[103],"subunits":[104,219],"20S":[107,192],"a":[110],"small":[111],"number":[112],"19S":[115,194,218,236],"regulatory":[116],"particle":[117],"proteins),":[118],"3":[120],"belonged":[121],"ubiquitin":[124],"system.":[125],"Sixty-two":[126],"interacting":[129],"(PIPs)":[131],"differentially":[133],"represented":[134,166],"CP":[136,226],"versus":[137,193,220],"EP,":[138],"with":[139,158],"folds":[140],"difference":[141],"ranging":[142],"1.5":[144],"4.8.":[146],"bioinformatics":[148],"revealed":[150],"that":[151,187,229,241],"functionally":[152],"most":[153],"EP-PIPs":[155],"associated":[157],"protein":[159],"biosynthesis":[160],"and,":[161],"unlike":[162],"CP-PIPs,":[163],"under":[165],"by":[167,181],"chaperon/ATP-binding":[168],"proteins.":[169],"Identities":[170],"some":[172],"PIPs":[178],"verified":[180],"Western":[182],"blotting.":[183],"Importantly,":[184],"uncovered":[186],"stoichiometry":[189],"was":[200],"different":[201],"one":[205],"calculated":[206],"intracellular":[209],"proteasomes.":[210],"Specifically,":[211],"EP":[213],"prep":[214],"contained":[215],"only":[216],"three":[217],"at":[221],"least":[222],"18":[223],"one,":[227],"suggesting":[228],"deficient":[234],"complexes,":[237],"may":[239],"imply":[240],"they":[242],"special":[244],"biological":[245],"functions.":[246],"J.":[247],"Cell.":[248],"Physiol.":[249],"232:":[250],"842-851,":[251],"2017.":[252],"\u00a9":[253],"2016":[254],"Wiley":[255],"Periodicals,":[256],"Inc.":[257]},"counts_by_year":[{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":2}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-08-23T00:00:00"}