{"id":"https://openalex.org/W2484257584","doi":"https://doi.org/10.1186/s13007-016-0137-3","title":"In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections","display_name":"In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections","publication_year":2016,"publication_date":"2016-07-27","ids":{"openalex":"https://openalex.org/W2484257584","doi":"https://doi.org/10.1186/s13007-016-0137-3","mag":"2484257584","pmid":"https://pubmed.ncbi.nlm.nih.gov/27471544","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4964054"},"language":"en","primary_location":{"id":"doi:10.1186/s13007-016-0137-3","doi":"https://doi.org/10.1186/s13007-016-0137-3","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13007-016-0137-3","pdf_url":"https://plantmethods.biomedcentral.com/track/pdf/10.1186/s13007-016-0137-3","source":{"id":"https://openalex.org/S184470774","display_name":"Plant Methods","issn_l":"1746-4811","issn":["1746-4811"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://plantmethods.biomedcentral.com/track/pdf/10.1186/s13007-016-0137-3","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081295801","display_name":"Mitchell A. Ellison","orcid":"https://orcid.org/0000-0002-5682-096X","observed_orcids":["https://orcid.org/0000-0002-5682-096X"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mitchell A. Ellison","raw_affiliation_strings":["Department of Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103702115","display_name":"Michael B. McMahon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael B. McMahon","raw_affiliation_strings":["USDA-ARS Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USDA-ARS Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, USA","institution_ids":["https://openalex.org/I1312222531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072431229","display_name":"Morris R. Bonde","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Morris R. Bonde","raw_affiliation_strings":["USDA-ARS Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USDA-ARS Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, USA","institution_ids":["https://openalex.org/I1312222531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056549023","display_name":"Cristi L. Palmer","orcid":"https://orcid.org/0000-0003-4256-3613","observed_orcids":["https://orcid.org/0000-0003-4256-3613"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cristi L. Palmer","raw_affiliation_strings":["IR-4 Project, Rutgers University, Princeton, NJ, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IR-4 Project, Rutgers University, Princeton, NJ, USA","institution_ids":["https://openalex.org/I102322142"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008858321","display_name":"Douglas G. Luster","orcid":"https://orcid.org/0000-0002-5789-408X","observed_orcids":["https://orcid.org/0000-0002-5789-408X"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Douglas G. Luster","raw_affiliation_strings":["USDA-ARS Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, USA"],"raw_orcid":"https://orcid.org/0000-0002-5789-408X","affiliations":[{"raw_affiliation_string":"USDA-ARS Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, USA","institution_ids":["https://openalex.org/I1312222531"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5008858321"],"corresponding_institution_ids":["https://openalex.org/I1312222531"],"apc_list":{"value":3090,"currency":"USD","value_usd":3090},"apc_paid":{"value":3090,"currency":"USD","value_usd":3090},"fwci":0.8657,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.74895104,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"12","issue":"1","first_page":"37","last_page":"37"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12668","display_name":"Yeasts and Rust Fungi Studies","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12668","display_name":"Yeasts and Rust Fungi Studies","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10733","display_name":"Wheat and Barley Genetics and Pathology","score":0.008100000210106373,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rust-fungi","display_name":"rust fungi","score":0.9739999771118164},{"id":"https://openalex.org/keywords/in-situ-hybridization","display_name":"in situ hybridization","score":0.9760000109672546},{"id":"https://openalex.org/keywords/paraffin-sections","display_name":"paraffin sections","score":0.7670000195503235},{"id":"https://openalex.org/keywords/puccinia-horiana","display_name":"Puccinia horiana","score":0.8899999856948853},{"id":"https://openalex.org/keywords/phakopsora-pachyrhizi","display_name":"Phakopsora pachyrhizi","score":0.9950000047683716},{"id":"https://openalex.org/keywords/chrysanthemum-morifolium","display_name":"Chrysanthemum morifolium","score":0.8809999823570251},{"id":"https://openalex.org/keywords/soybean","display_name":"soybean","score":0.9980000257492065},{"id":"https://openalex.org/keywords/plant-pathogenic-fungi","display_name":"plant pathogenic fungi","score":0.7940000295639038},{"id":"https://openalex.org/keywords/host-pathogen-interaction","display_name":"host-pathogen interaction","score":0.671999990940094}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.860931396484375},{"id":"https://openalex.org/C197781089","wikidata":"https://www.wikidata.org/wiki/Q575650","display_name":"Rust (programming language)","level":2,"score":0.7678767442703247},{"id":"https://openalex.org/C174618031","wikidata":"https://www.wikidata.org/wiki/Q177332","display_name":"Spore","level":2,"score":0.7193564176559448},{"id":"https://openalex.org/C2777570262","wikidata":"https://www.wikidata.org/wiki/Q7180218","display_name":"Phakopsora pachyrhizi","level":3,"score":0.5919579863548279},{"id":"https://openalex.org/C2776766014","wikidata":"https://www.wikidata.org/wiki/Q305162","display_name":"Puccinia","level":3,"score":0.5768348574638367},{"id":"https://openalex.org/C113670536","wikidata":"https://www.wikidata.org/wiki/Q7900365","display_name":"Urediniospore","level":3,"score":0.5551425218582153},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.55096036195755},{"id":"https://openalex.org/C126831891","wikidata":"https://www.wikidata.org/wiki/Q221673","display_name":"Host (biology)","level":2,"score":0.4943685531616211},{"id":"https://openalex.org/C99498324","wikidata":"https://www.wikidata.org/wiki/Q7075151","display_name":"Obligate parasite","level":3,"score":0.4730607271194458},{"id":"https://openalex.org/C2992076609","wikidata":"https://www.wikidata.org/wiki/Q968604","display_name":"Plant tissue","level":2,"score":0.4491996169090271},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.33916789293289185},{"id":"https://openalex.org/C74600772","wikidata":"https://www.wikidata.org/wiki/Q193237","display_name":"Fungicide","level":2,"score":0.13389578461647034},{"id":"https://openalex.org/C2779669290","wikidata":"https://www.wikidata.org/wiki/Q1220207","display_name":"Mildew","level":2,"score":0.10031339526176453},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1186/s13007-016-0137-3","doi":"https://doi.org/10.1186/s13007-016-0137-3","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13007-016-0137-3","pdf_url":"https://plantmethods.biomedcentral.com/track/pdf/10.1186/s13007-016-0137-3","source":{"id":"https://openalex.org/S184470774","display_name":"Plant Methods","issn_l":"1746-4811","issn":["1746-4811"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3924947","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4964054","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4964054","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4964054","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"Text"},{"id":"pmid:27471544","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27471544","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant methods","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1186/s13007-016-0137-3","doi":"https://doi.org/10.1186/s13007-016-0137-3","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13007-016-0137-3","pdf_url":"https://plantmethods.biomedcentral.com/track/pdf/10.1186/s13007-016-0137-3","source":{"id":"https://openalex.org/S184470774","display_name":"Plant Methods","issn_l":"1746-4811","issn":["1746-4811"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Methods","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.40450000762939453}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4318231123","display_name":null,"funder_award_id":"10007","funder_id":"https://openalex.org/F4320332778","funder_display_name":"Animal and Plant Health Inspection Service"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320332605","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044"},{"id":"https://openalex.org/F4320332778","display_name":"Animal and Plant Health Inspection Service","ror":"https://ror.org/0599wfz09"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2484257584.pdf","grobid_xml":"https://content.openalex.org/works/W2484257584.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W313547275","https://openalex.org/W1844237590","https://openalex.org/W1967845827","https://openalex.org/W1972824801","https://openalex.org/W1980292565","https://openalex.org/W2008354532","https://openalex.org/W2010680821","https://openalex.org/W2014311775","https://openalex.org/W2015933410","https://openalex.org/W2019374755","https://openalex.org/W2025048783","https://openalex.org/W2028082957","https://openalex.org/W2028409921","https://openalex.org/W2029409775","https://openalex.org/W2030256740","https://openalex.org/W2033860372","https://openalex.org/W2045356329","https://openalex.org/W2053543375","https://openalex.org/W2066449847","https://openalex.org/W2067612240","https://openalex.org/W2069644796","https://openalex.org/W2088687577","https://openalex.org/W2099073787","https://openalex.org/W2114115955","https://openalex.org/W2118751052","https://openalex.org/W2120034611","https://openalex.org/W2133547418","https://openalex.org/W2151556630","https://openalex.org/W2165530387","https://openalex.org/W2226570905","https://openalex.org/W2228399582","https://openalex.org/W2327959256","https://openalex.org/W2332507248","https://openalex.org/W2742293624","https://openalex.org/W2884519307","https://openalex.org/W3023655498","https://openalex.org/W6636630185"],"related_works":["https://openalex.org/W7098731209","https://openalex.org/W2315444531","https://openalex.org/W2327195940","https://openalex.org/W2001920062","https://openalex.org/W2404284323","https://openalex.org/W2074645243","https://openalex.org/W2203548845","https://openalex.org/W2092309644","https://openalex.org/W2167261997","https://openalex.org/W2029409775"],"abstract_inverted_index":{"BACKGROUND:":[0],"Rust":[1],"fungi":[2,48,143,149,170,191],"are":[3,23,35],"obligate":[4],"pathogens":[5,22,128,164],"with":[6,26,37,91,98,105,171],"multiple":[7,16],"life":[8],"stages":[9],"often":[10,24],"including":[11],"different":[12,166],"spore":[13],"types":[14],"and":[15,49,101,129,144],"plant":[17,33,62,133,147,176,189,198],"hosts.":[18],"While":[19],"individual":[20],"rust":[21,38,127,142,169],"associated":[25],"specific":[27],"plants,":[28,52],"a":[29],"wide":[30],"range":[31],"of":[32,86,117,168,175,182,188,196],"species":[34],"infected":[36,90,97,104],"fungi.":[39],"To":[40,78],"study":[41,187],"the":[42,69,82,180,186],"interactions":[43],"between":[44,126],"these":[45,118],"important":[46],"pathogenic":[47,148,190],"their":[50,130],"host":[51,132,197],"one":[53],"must":[54],"be":[55,66,161],"able":[56],"to":[57,141,163],"differentiate":[58],"fungal":[59],"tissue":[60,113],"from":[61,165],"tissue.":[63,177,199],"This":[64,136],"can":[65,160],"accomplished":[67],"using":[68,81],"In":[70],"situ":[71],"hybridization":[72],"(ISH)":[73],"protocol":[74,138,159,184],"described":[75],"here.":[76],"RESULTS:":[77],"validate":[79],"reproducibility":[80],"ISH":[83,137],"protocol,":[84],"samples":[85],"Chrysanthemum":[87],"\u00d7":[88,95],"morifolium":[89],"Puccinia":[92],"horiana,":[93],"Gladiolus":[94],"hortulanus":[96],"Uromyces":[99],"transversalis":[100],"Glycine":[102],"max":[103],"Phakopsora":[106],"pachyrhizi":[107],"were":[108],"tested":[109],"alongside":[110],"uninfected":[111],"leaf":[112],"samples.":[114],"The":[115],"results":[116],"tests":[119],"show":[120],"that":[121,157],"this":[122,158,183],"technique":[123],"clearly":[124],"distinguishes":[125],"respective":[131],"tissues.":[134],"CONCLUSIONS:":[135],"is":[139],"applicable":[140],"potentially":[145],"other":[146],"as":[150],"well.":[151],"It":[152],"has":[153],"been":[154],"shown":[155],"here":[156],"applied":[162],"genera":[167],"no":[172],"background":[173],"staining":[174],"We":[178],"encourage":[179],"use":[181],"for":[185],"in":[192],"paraffin":[193],"embedded":[194],"sections":[195]},"counts_by_year":[{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-08-23T00:00:00"}