{"id":"https://openalex.org/W2474630786","doi":"https://doi.org/10.1007/978-1-4939-3542-0_2","title":"Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation","display_name":"Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation","publication_year":2016,"publication_date":"2016-01-01","ids":{"openalex":"https://openalex.org/W2474630786","doi":"https://doi.org/10.1007/978-1-4939-3542-0_2","mag":"2474630786","pmid":"https://pubmed.ncbi.nlm.nih.gov/27193840"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-4939-3542-0_2","doi":"https://doi.org/10.1007/978-1-4939-3542-0_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4939-3542-0_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2477318079","display_name":"The Mitotic Spindle"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Bin He","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bin He","raw_affiliation_strings":["Department of Biological Sciences, Biocomplexity Institute, Virginia Tech, 1015 Life Science Circle, Blacksburg, VA, 24061, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Biocomplexity Institute, Virginia Tech, 1015 Life Science Circle, Blacksburg, VA, 24061, USA","institution_ids":["https://openalex.org/I859038795"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026937109","display_name":"Daniela Cimini","orcid":"https://orcid.org/0000-0002-4082-4894","observed_orcids":["https://orcid.org/0000-0002-4082-4894"]},"institutions":[{"id":"https://openalex.org/I859038795","display_name":"Virginia Tech","ror":"https://ror.org/02smfhw86","country_code":"US","type":"education","lineage":["https://openalex.org/I859038795"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Daniela Cimini","raw_affiliation_strings":["Department of Biological Sciences, Biocomplexity Institute, Virginia Tech, 1015 Life Science Circle, Blacksburg, VA, 24061, USA. cimini@vt.edu","Department of Biological Sciences, Biocomplexity Institute, Virginia Tech, 1015 Life Science Circle, Blacksburg, VA, 24061, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Biocomplexity Institute, Virginia Tech, 1015 Life Science Circle, Blacksburg, VA, 24061, USA. cimini@vt.edu","institution_ids":["https://openalex.org/I859038795"]},{"raw_affiliation_string":"Department of Biological Sciences, Biocomplexity Institute, Virginia Tech, 1015 Life Science Circle, Blacksburg, VA, 24061, USA","institution_ids":["https://openalex.org/I859038795"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5026937109"],"corresponding_institution_ids":["https://openalex.org/I859038795"],"apc_list":null,"apc_paid":null,"fwci":0.4088,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.532,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"1413","issue":null,"first_page":"15","last_page":"31"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10492","display_name":"Microtubule and mitosis dynamics","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/photoactivatable-gfp","display_name":"photoactivatable GFP","score":0.878000020980835},{"id":"https://openalex.org/keywords/microtubule-dynamics","display_name":"microtubule dynamics","score":0.9869999885559082},{"id":"https://openalex.org/keywords/chromosome-segregation","display_name":"chromosome segregation","score":0.9950000047683716},{"id":"https://openalex.org/keywords/mitosis","display_name":"mitosis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/chromosome-movement","display_name":"chromosome movement","score":0.8130000233650208},{"id":"https://openalex.org/keywords/cell-division","display_name":"cell division","score":0.5389999747276306}],"concepts":[{"id":"https://openalex.org/C8502773","wikidata":"https://www.wikidata.org/wiki/Q2066168","display_name":"Sister chromatids","level":4,"score":0.8659936785697937},{"id":"https://openalex.org/C2778354886","wikidata":"https://www.wikidata.org/wiki/Q5113927","display_name":"Chromosome segregation","level":4,"score":0.7668999433517456},{"id":"https://openalex.org/C93304396","wikidata":"https://www.wikidata.org/wiki/Q14763008","display_name":"Mitosis","level":2,"score":0.7605996131896973},{"id":"https://openalex.org/C20418707","wikidata":"https://www.wikidata.org/wiki/Q189933","display_name":"Microtubule","level":2,"score":0.6395595073699951},{"id":"https://openalex.org/C30481170","wikidata":"https://www.wikidata.org/wiki/Q37748","display_name":"Chromosome","level":3,"score":0.6040478944778442},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.546691358089447},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5234267711639404},{"id":"https://openalex.org/C85813293","wikidata":"https://www.wikidata.org/wiki/Q188909","display_name":"Cell division","level":3,"score":0.5029165148735046},{"id":"https://openalex.org/C73397532","wikidata":"https://www.wikidata.org/wiki/Q623749","display_name":"Chromatid","level":4,"score":0.44311439990997314},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4247041642665863},{"id":"https://openalex.org/C47432576","wikidata":"https://www.wikidata.org/wiki/Q918594","display_name":"Spindle apparatus","level":4,"score":0.4129071831703186},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.24013519287109375},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12485620379447937}],"mesh":[{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008027","descriptor_name":"Light","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008870","descriptor_name":"Microtubules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008938","descriptor_name":"Mitosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015533","descriptor_name":"Transcriptional Activation","qualifier_ui":"Q000528","qualifier_name":"radiation effects","is_major_topic":true},{"descriptor_ui":"D020090","descriptor_name":"Chromosome Segregation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D059008","descriptor_name":"Time-Lapse Imaging","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-4939-3542-0_2","doi":"https://doi.org/10.1007/978-1-4939-3542-0_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4939-3542-0_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:27193840","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27193840","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320337397","display_name":"Division of Molecular and Cellular Biosciences","ror":"https://ror.org/002jdaq33"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":52,"referenced_works":["https://openalex.org/W1494673328","https://openalex.org/W1522090640","https://openalex.org/W1529934215","https://openalex.org/W1567040584","https://openalex.org/W1631125692","https://openalex.org/W1631508394","https://openalex.org/W1813351491","https://openalex.org/W1915934342","https://openalex.org/W1966306002","https://openalex.org/W1966669440","https://openalex.org/W1975648894","https://openalex.org/W1976108509","https://openalex.org/W1988326309","https://openalex.org/W1995940821","https://openalex.org/W1999699954","https://openalex.org/W2000838642","https://openalex.org/W2008923545","https://openalex.org/W2017518375","https://openalex.org/W2042107535","https://openalex.org/W2043890495","https://openalex.org/W2050415386","https://openalex.org/W2050953095","https://openalex.org/W2057286714","https://openalex.org/W2058384571","https://openalex.org/W2067126148","https://openalex.org/W2068427957","https://openalex.org/W2069195647","https://openalex.org/W2070026305","https://openalex.org/W2073057352","https://openalex.org/W2075081297","https://openalex.org/W2076814468","https://openalex.org/W2080308138","https://openalex.org/W2081406767","https://openalex.org/W2087625931","https://openalex.org/W2088006036","https://openalex.org/W2094168229","https://openalex.org/W2098676093","https://openalex.org/W2102502996","https://openalex.org/W2102960342","https://openalex.org/W2105353553","https://openalex.org/W2112514675","https://openalex.org/W2115918652","https://openalex.org/W2119826873","https://openalex.org/W2124699072","https://openalex.org/W2129124130","https://openalex.org/W2132903556","https://openalex.org/W2142128650","https://openalex.org/W2148148905","https://openalex.org/W2153304690","https://openalex.org/W2170515091","https://openalex.org/W2495081311","https://openalex.org/W2518697824"],"related_works":["https://openalex.org/W2027315090","https://openalex.org/W2084300745","https://openalex.org/W816150493","https://openalex.org/W1980419839","https://openalex.org/W2810602752","https://openalex.org/W1546121533","https://openalex.org/W1510246089","https://openalex.org/W2810660275","https://openalex.org/W1967395625","https://openalex.org/W1798535792"],"abstract_inverted_index":null,"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-07-22T00:00:00"}