{"id":"https://openalex.org/W2473581799","doi":null,"title":"Effect of tyrosine hydroxylase gene silencing in CD4+ T lymphocytes on differentiation and function of helper T cells.","display_name":"Effect of tyrosine hydroxylase gene silencing in CD4+ T lymphocytes on differentiation and function of helper T cells.","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W2473581799","mag":"2473581799","pmid":"https://pubmed.ncbi.nlm.nih.gov/23160220"},"language":"en","primary_location":{"id":"pmid:23160220","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23160220","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neuro endocrinology letters","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100351050","display_name":"Yan Liu","orcid":"https://orcid.org/0000-0002-1378-1825","observed_orcids":["https://orcid.org/0000-0002-1378-1825"]},"institutions":[{"id":"https://openalex.org/I199305430","display_name":"Nantong University","ror":"https://ror.org/02afcvw97","country_code":"CN","type":"education","lineage":["https://openalex.org/I199305430"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yan Liu","raw_affiliation_strings":["Department of Physiology, School of Medicine, Nantong University, Nantong, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, School of Medicine, Nantong University, Nantong, China","institution_ids":["https://openalex.org/I199305430"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100456182","display_name":"Yan Huang","orcid":"https://orcid.org/0000-0002-0126-7810","observed_orcids":["https://orcid.org/0000-0002-0126-7810"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yan Huang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100403889","display_name":"Xiaoqin Wang","orcid":"https://orcid.org/0000-0001-9779-5089","observed_orcids":["https://orcid.org/0000-0001-9779-5089"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xiao-Qin Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029563117","display_name":"Yu-Ping Peng","orcid":"https://orcid.org/0000-0002-5966-0922","observed_orcids":["https://orcid.org/0000-0002-5966-0922"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yu-Ping Peng","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5089859116","display_name":"Yihua Qiu","orcid":"https://orcid.org/0000-0003-1243-4810","observed_orcids":["https://orcid.org/0000-0003-1243-4810"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yi-Hua Qiu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3895,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.56605364,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"33","issue":"6","first_page":"643","last_page":"50"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10469","display_name":"Psoriasis: Treatment and Pathogenesis","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14072","display_name":"Immunotoxicology and immune responses","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tyrosine-hydroxylase","display_name":"tyrosine hydroxylase","score":1.0},{"id":"https://openalex.org/keywords/cd4-t-cells","display_name":"CD4+ T cells","score":0.8109999895095825},{"id":"https://openalex.org/keywords/t-helper-cell-differentiation","display_name":"T helper cell differentiation","score":0.9049999713897705},{"id":"https://openalex.org/keywords/catecholamines","display_name":"catecholamines","score":0.8220000267028809},{"id":"https://openalex.org/keywords/th1-th2-polarization","display_name":"Th1/Th2 polarization","score":0.8399999737739563},{"id":"https://openalex.org/keywords/interferon-gamma","display_name":"interferon-gamma","score":0.8650000095367432},{"id":"https://openalex.org/keywords/il-4","display_name":"IL-4","score":0.9990000128746033},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.578000009059906},{"id":"https://openalex.org/keywords/norepinephrine","display_name":"norepinephrine","score":0.75},{"id":"https://openalex.org/keywords/epinephrine","display_name":"epinephrine","score":0.9810000061988831},{"id":"https://openalex.org/keywords/dopamine","display_name":"dopamine","score":0.8230000138282776},{"id":"https://openalex.org/keywords/cytokine-production","display_name":"cytokine production","score":0.7089999914169312}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.637760579586029},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.6228144764900208},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5674834847450256},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5647591948509216},{"id":"https://openalex.org/C159167319","wikidata":"https://www.wikidata.org/wiki/Q420766","display_name":"Tyrosine hydroxylase","level":3,"score":0.558378279209137},{"id":"https://openalex.org/C2992881475","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T lymphocyte","level":3,"score":0.4377315044403076},{"id":"https://openalex.org/C131665280","wikidata":"https://www.wikidata.org/wiki/Q14866169","display_name":"Interleukin 3","level":5,"score":0.418060839176178},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4108573794364929},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.38510724902153015},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.361674964427948},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.34422019124031067},{"id":"https://openalex.org/C129374314","wikidata":"https://www.wikidata.org/wiki/Q909381","display_name":"Interleukin 21","level":4,"score":0.3403412997722626},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.2731945514678955},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.20480531454086304},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17470625042915344},{"id":"https://openalex.org/C513476851","wikidata":"https://www.wikidata.org/wiki/Q170304","display_name":"Dopamine","level":2,"score":0.13903170824050903},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08397665619850159},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.07846510410308838},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002395","descriptor_name":"Catecholamines","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007371","descriptor_name":"Interferon-gamma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008198","descriptor_name":"Lymph Nodes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014446","descriptor_name":"Tyrosine 3-Monooxygenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014446","descriptor_name":"Tyrosine 3-Monooxygenase","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D014446","descriptor_name":"Tyrosine 3-Monooxygenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015496","descriptor_name":"CD4-Positive T-Lymphocytes","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D015496","descriptor_name":"CD4-Positive T-Lymphocytes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D015847","descriptor_name":"Interleukin-4","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015971","descriptor_name":"Gene Expression Regulation, Enzymologic","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D018417","descriptor_name":"Th1 Cells","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D018417","descriptor_name":"Th1 Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D018418","descriptor_name":"Th2 Cells","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D018418","descriptor_name":"Th2 Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:23160220","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23160220","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neuro endocrinology letters","raw_type":"Journal Article"},{"id":"mag:2473581799","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23160220","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8371999859809875,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W148782277","https://openalex.org/W1579600696","https://openalex.org/W1965039301","https://openalex.org/W1969267103","https://openalex.org/W1970448065","https://openalex.org/W1975994431","https://openalex.org/W1987912134","https://openalex.org/W2002611075","https://openalex.org/W2004561060","https://openalex.org/W2020954963","https://openalex.org/W2021810330","https://openalex.org/W2025139014","https://openalex.org/W2030853294","https://openalex.org/W2041858687","https://openalex.org/W2045236761","https://openalex.org/W2045360275","https://openalex.org/W2052183164","https://openalex.org/W2059247237","https://openalex.org/W2059459516","https://openalex.org/W2063391264","https://openalex.org/W2077088309","https://openalex.org/W2078707624","https://openalex.org/W2080744746","https://openalex.org/W2081149174","https://openalex.org/W2081642059","https://openalex.org/W2084790398","https://openalex.org/W2095213901","https://openalex.org/W2105808377","https://openalex.org/W2112670248","https://openalex.org/W2117197980","https://openalex.org/W2120468520","https://openalex.org/W2125747435","https://openalex.org/W2135514207","https://openalex.org/W2143311046"],"related_works":["https://openalex.org/W2422244658","https://openalex.org/W2083644583","https://openalex.org/W2418336610","https://openalex.org/W2118508906","https://openalex.org/W2020954963","https://openalex.org/W2092266965","https://openalex.org/W1835762668","https://openalex.org/W3117509745","https://openalex.org/W2062567403","https://openalex.org/W2091329062"],"abstract_inverted_index":{"OBJECTIVES:":[0],"We":[1],"explored":[2],"effect":[3],"of":[4,7,16,27,39,55,98,107,120,162,171,177,189,214,243],"gene":[5,89,164,228],"silencing":[6,161,229],"tyrosine":[8],"hydroxylase":[9],"(TH),":[10],"a":[11],"rate-limiting":[12],"enzyme":[13],"for":[14,36,80,87],"synthesis":[15,235],"catecholamines":[17],"(CAs),":[18],"in":[19,117,165,210,236],"CD4+":[20,43,72,123,133,166,217,237],"T":[21,29,44,73,124,134,167,218,238],"cells":[22,31,84,104,125,156,173,179],"on":[23],"differentiation":[24,244],"and":[25,48,65,90,93,101,105,115,143,145,152,174,197,206,233,240,245],"function":[26,246],"helper":[28],"(Th)":[30],"to":[32,180],"provide":[33],"more":[34],"evidence":[35],"functional":[37],"significance":[38],"lymphocyte-derived":[40],"CAs.":[41],"METHODS:":[42],"lymphocytes":[45,74,135,168,219,239],"were":[46,85,126,199,208,221],"isolated":[47],"purified":[49],"from":[50],"the":[51,202,211],"mesenteric":[52],"lymph":[53],"nodes":[54],"mice.":[56],"Recombinant":[57],"TH":[58,88,137,141,163,224,227,231],"miRNA":[59],"expression":[60,92,232],"vector":[61],"(pcDNA6.2-GW/EmGFPmiR-TH)":[62],"was":[63],"constructed":[64],"transfected":[66,222],"into":[67],"concanavalin":[68],"A":[69],"(Con":[70],"A)-activated":[71],"using":[75],"nucleofection":[76],"technology.":[77],"After":[78],"incubated":[79],"48":[81],"h,":[82],"these":[83],"detected":[86],"protein":[91,144],"CA":[94,234],"content.":[95],"Simultaneously,":[96],"percentage":[97,170],"interferon-\u03b3":[99],"(IFN-\u03b3)-":[100],"interleukin-4":[102],"(IL-4)-producing":[103],"levels":[106],"IL-2,":[108,195],"IFN-\u03b3,":[109],"tumor":[110],"necrosis":[111],"factor":[112],"(TNF),":[113],"IL-4":[114,205],"IL-5":[116],"culture":[118,212],"supernatants":[119,213],"Con":[121,215],"A-stimulated":[122,216],"examined":[127],"by":[128],"flow":[129],"cytometric":[130],"analysis.":[131],"RESULTS:":[132],"with":[136,157,223],"RNAi":[138],"expressed":[139],"less":[140,147],"mRNA":[142],"synthesized":[146],"CAs":[148],"including":[149],"norepinephrine,":[150],"epinephrine":[151],"dopamine":[153],"than":[154],"control":[155],"mock":[158],"transfection.":[159],"The":[160,192],"reduced":[169],"IL-4-producing":[172,181],"elevated":[175],"ratio":[176],"IFN-\u03b3-producing":[178,190],"cells,":[182],"although":[183],"it":[184],"did":[185],"not":[186],"alter":[187],"proportion":[188],"cells.":[191,249],"Th1":[193,248],"cytokines,":[194,204],"IFN-\u03b3":[196],"TNF,":[198],"increased,":[200],"but":[201],"Th2":[203],"IL-5,":[207],"decreased":[209],"that":[220],"miRNA.":[225],"CONCLUSION:":[226],"attenuates":[230],"promotes":[241],"polarization":[242],"towards":[247]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-07-22T00:00:00"}