{"id":"https://openalex.org/W2472004504","doi":"https://doi.org/10.1002/humu.23051","title":"Fourth Generation of Next\u2010Generation Sequencing Technologies: Promise and Consequences","display_name":"Fourth Generation of Next\u2010Generation Sequencing Technologies: Promise and Consequences","publication_year":2016,"publication_date":"2016-07-13","ids":{"openalex":"https://openalex.org/W2472004504","doi":"https://doi.org/10.1002/humu.23051","mag":"2472004504","pmid":"https://pubmed.ncbi.nlm.nih.gov/27406789","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5111608"},"language":"en","primary_location":{"id":"doi:10.1002/humu.23051","doi":"https://doi.org/10.1002/humu.23051","is_oa":true,"landing_page_url":"https://doi.org/10.1002/humu.23051","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/humu.23051","source":{"id":"https://openalex.org/S98809561","display_name":"Human Mutation","issn_l":"1059-7794","issn":["1059-7794","1098-1004"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human Mutation","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/humu.23051","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043653563","display_name":"Rongqin Ke","orcid":"https://orcid.org/0000-0002-9868-3713","observed_orcids":["https://orcid.org/0000-0002-9868-3713"]},"institutions":[{"id":"https://openalex.org/I119045251","display_name":"Huaqiao University","ror":"https://ror.org/03frdh605","country_code":"CN","type":"education","lineage":["https://openalex.org/I119045251"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Rongqin Ke","raw_affiliation_strings":["School of Biomedical Sciences, Huaqiao University, Quanzhou, Fujian, 362021 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biomedical Sciences, Huaqiao University, Quanzhou, Fujian, 362021 China","institution_ids":["https://openalex.org/I119045251"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023369695","display_name":"Marco Mignardi","orcid":"https://orcid.org/0000-0002-9430-2442","observed_orcids":["https://orcid.org/0000-0002-9430-2442"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]},{"id":"https://openalex.org/I2800139495","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22","country_code":"SE","type":"facility","lineage":["https://openalex.org/I2800139495"]},{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["SE","US"],"is_corresponding":false,"raw_author_name":"Marco Mignardi","raw_affiliation_strings":["Department of Bioengineering, Stanford University, Stanford, California, 75105","Department of Information Technology, Centre for Image Analysis, Science for Life Laboratory, Uppsala University, Uppsala, SE-75105, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Stanford University, Stanford, California, 75105","institution_ids":["https://openalex.org/I97018004"]},{"raw_affiliation_string":"Department of Information Technology, Centre for Image Analysis, Science for Life Laboratory, Uppsala University, Uppsala, SE-75105, Sweden","institution_ids":["https://openalex.org/I123387679","https://openalex.org/I2800139495"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029278543","display_name":"Thomas Hauling","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161593684","display_name":"Stockholm University","ror":"https://ror.org/05f0yaq80","country_code":"SE","type":"education","lineage":["https://openalex.org/I161593684"]},{"id":"https://openalex.org/I2800139495","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22","country_code":"SE","type":"facility","lineage":["https://openalex.org/I2800139495"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Thomas Hauling","raw_affiliation_strings":["Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Solna, SE-171 21, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Solna, SE-171 21, Sweden","institution_ids":["https://openalex.org/I161593684","https://openalex.org/I2800139495"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002137026","display_name":"Mats F. Nilsson","orcid":"https://orcid.org/0000-0001-9985-0387","observed_orcids":["https://orcid.org/0000-0001-9985-0387"]},"institutions":[{"id":"https://openalex.org/I161593684","display_name":"Stockholm University","ror":"https://ror.org/05f0yaq80","country_code":"SE","type":"education","lineage":["https://openalex.org/I161593684"]},{"id":"https://openalex.org/I2800139495","display_name":"Science for Life Laboratory","ror":"https://ror.org/04ev03g22","country_code":"SE","type":"facility","lineage":["https://openalex.org/I2800139495"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Mats Nilsson","raw_affiliation_strings":["Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Solna, SE-171 21, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Solna, SE-171 21, Sweden","institution_ids":["https://openalex.org/I161593684","https://openalex.org/I2800139495"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5002137026"],"corresponding_institution_ids":["https://openalex.org/I161593684","https://openalex.org/I2800139495"],"apc_list":{"value":2740,"currency":"USD","value_usd":2740},"apc_paid":{"value":2740,"currency":"USD","value_usd":2740},"fwci":0.5739,"has_fulltext":true,"cited_by_count":65,"citation_normalized_percentile":{"value":0.50065732,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"37","issue":"12","first_page":"1363","last_page":"1367"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.923799991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.923799991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.06030000001192093,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/spatial-transcriptomics","display_name":"spatial transcriptomics","score":0.7760000228881836},{"id":"https://openalex.org/keywords/in-situ-sequencing","display_name":"in situ sequencing","score":0.7860000133514404},{"id":"https://openalex.org/keywords/single-cell-rna-sequencing","display_name":"single-cell RNA sequencing","score":0.5479999780654907},{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.6809999942779541},{"id":"https://openalex.org/keywords/cancer-research","display_name":"cancer research","score":0.6179999709129333},{"id":"https://openalex.org/keywords/brain-function","display_name":"brain function","score":0.5690000057220459},{"id":"https://openalex.org/keywords/fixed-cells","display_name":"fixed cells","score":0.6399999856948853},{"id":"https://openalex.org/keywords/cancer-diagnosis","display_name":"cancer diagnosis","score":0.5419999957084656}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7995429039001465},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.7620377540588379},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6668892502784729},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.6145017743110657},{"id":"https://openalex.org/C132917006","wikidata":"https://www.wikidata.org/wiki/Q5250436","display_name":"Deep sequencing","level":4,"score":0.46920910477638245},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.32250910997390747},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.2936548590660095},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.27224084734916687},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.24320298433303833},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1639936864376068},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D020022","descriptor_name":"Genetic Predisposition to Disease","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1002/humu.23051","doi":"https://doi.org/10.1002/humu.23051","is_oa":true,"landing_page_url":"https://doi.org/10.1002/humu.23051","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/humu.23051","source":{"id":"https://openalex.org/S98809561","display_name":"Human Mutation","issn_l":"1059-7794","issn":["1059-7794","1098-1004"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human Mutation","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:4076092","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5111608","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5111608","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5111608","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hum Mutat","raw_type":"Text"},{"id":"pmid:27406789","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27406789","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human mutation","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1002/humu.23051","doi":"https://doi.org/10.1002/humu.23051","is_oa":true,"landing_page_url":"https://doi.org/10.1002/humu.23051","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/humu.23051","source":{"id":"https://openalex.org/S98809561","display_name":"Human Mutation","issn_l":"1059-7794","issn":["1059-7794","1098-1004"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human Mutation","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322581","display_name":"Vetenskapsr\u00e5det","ror":"https://ror.org/03zttf063"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2472004504.pdf","grobid_xml":"https://content.openalex.org/works/W2472004504.grobid-xml"},"referenced_works_count":39,"referenced_works":["https://openalex.org/W54859794","https://openalex.org/W1520883967","https://openalex.org/W1606634502","https://openalex.org/W1813068103","https://openalex.org/W1873696567","https://openalex.org/W1921741111","https://openalex.org/W1972618510","https://openalex.org/W1973950074","https://openalex.org/W1977544680","https://openalex.org/W1990601349","https://openalex.org/W1991872434","https://openalex.org/W2023887100","https://openalex.org/W2033072655","https://openalex.org/W2042789810","https://openalex.org/W2051036306","https://openalex.org/W2055655674","https://openalex.org/W2066789848","https://openalex.org/W2069089843","https://openalex.org/W2083048982","https://openalex.org/W2097369455","https://openalex.org/W2105583187","https://openalex.org/W2109847775","https://openalex.org/W2112138633","https://openalex.org/W2118782378","https://openalex.org/W2123273050","https://openalex.org/W2125336256","https://openalex.org/W2126537932","https://openalex.org/W2131629478","https://openalex.org/W2140258573","https://openalex.org/W2151013106","https://openalex.org/W2159084616","https://openalex.org/W2159982756","https://openalex.org/W2163302580","https://openalex.org/W2166168429","https://openalex.org/W2167943254","https://openalex.org/W2195967731","https://openalex.org/W2207736799","https://openalex.org/W2406725268","https://openalex.org/W2471536144"],"related_works":["https://openalex.org/W1974154205","https://openalex.org/W3132643984","https://openalex.org/W2359210733","https://openalex.org/W7049439482","https://openalex.org/W3110500961","https://openalex.org/W4399816304","https://openalex.org/W3021728480","https://openalex.org/W2941036477","https://openalex.org/W7148734974","https://openalex.org/W2514011171"],"abstract_inverted_index":{"In":[0],"this":[1],"review,":[2],"we":[3],"discuss":[4,103],"the":[5,8,14,35,54,57,66,70,78,96,104,120,136,139,143],"emergence":[6],"of":[7,17,31,56,95,106,145],"fourth-generation":[9,63],"sequencing":[10,32,64,107,117,148],"technologies":[11],"that":[12,50,124,138],"preserve":[13],"spatial":[15],"coordinates":[16],"RNA":[18],"and":[19,80,92,122,135,149,156],"DNA":[20],"sequences":[21],"with":[22],"up":[23],"to":[24,34,52,68,86,129,131],"subcellular":[25],"resolution,":[26],"thus":[27],"enabling":[28],"back":[29],"mapping":[30],"reads":[33],"original":[36],"histological":[37],"context.":[38],"This":[39],"information":[40,140],"is":[41],"used,":[42],"for":[43,90],"example,":[44],"in":[45,60,84,99,111,146,154],"two":[46],"current":[47],"large-scale":[48],"projects":[49],"aim":[51],"unravel":[53],"function":[55],"brain.":[58],"Also":[59],"cancer":[61],"research,":[62],"has":[65,75],"potential":[67],"revolutionize":[69],"field.":[71],"Cancer":[72],"Research":[73],"UK":[74],"named":[76],"\"Mapping":[77],"molecular":[79],"cellular":[81],"tumor":[82,100],"microenvironment":[83],"order":[85],"define":[87],"new":[88,126],"targets":[89],"therapy":[91],"prognosis\"":[93],"one":[94],"grand":[97],"challenges":[98,123],"biology.":[101],"We":[102],"advantages":[105],"nucleic":[108],"acids":[109],"directly":[110],"fixed":[112],"cells":[113],"over":[114],"traditional":[115],"next-generation":[116],"(NGS)":[118],"methods,":[119],"limitations":[121],"these":[125],"methods":[127,151],"have":[128,153],"face":[130],"become":[132],"broadly":[133],"applicable,":[134],"impact":[137],"generated":[141],"by":[142],"combination":[144],"situ":[147],"NGS":[150],"will":[152],"research":[155],"diagnostics.":[157]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":11},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-07-22T00:00:00"}
