{"id":"https://openalex.org/W2467662986","doi":"https://doi.org/10.1016/j.cca.2016.06.039","title":"The impact of protein extraction protocols on the performance of currently available MALDI-TOF mass spectrometry for identification of mycobacterial clinical isolates cultured in liquid media","display_name":"The impact of protein extraction protocols on the performance of currently available MALDI-TOF mass spectrometry for identification of mycobacterial clinical isolates cultured in liquid media","publication_year":2016,"publication_date":"2016-07-03","ids":{"openalex":"https://openalex.org/W2467662986","doi":"https://doi.org/10.1016/j.cca.2016.06.039","mag":"2467662986","pmid":"https://pubmed.ncbi.nlm.nih.gov/27380997"},"language":"en","primary_location":{"id":"doi:10.1016/j.cca.2016.06.039","doi":"https://doi.org/10.1016/j.cca.2016.06.039","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.cca.2016.06.039","pdf_url":null,"source":{"id":"https://openalex.org/S207549998","display_name":"Clinica Chimica Acta","issn_l":"0009-8981","issn":["0009-8981","1873-3492"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinica Chimica Acta","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102795122","display_name":"Jeong Su Park","orcid":"https://orcid.org/0000-0001-5149-1362","observed_orcids":["https://orcid.org/0000-0001-5149-1362","https://orcid.org/0000-0003-0261-2185"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I2802835388","display_name":"Seoul National University Hospital","ror":"https://ror.org/01z4nnt86","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388"]},{"id":"https://openalex.org/I2803058125","display_name":"Seoul National University Bundang Hospital","ror":"https://ror.org/00cb3km46","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jeong Su Park","raw_affiliation_strings":["Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea"],"raw_orcid":"https://orcid.org/0000-0001-5149-1362","affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111877056","display_name":"Soon Hee Choi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2803058125","display_name":"Seoul National University Bundang Hospital","ror":"https://ror.org/00cb3km46","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Soon Hee Choi","raw_affiliation_strings":["Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea","institution_ids":["https://openalex.org/I2803058125"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009057220","display_name":"Sang Mee Hwang","orcid":"https://orcid.org/0000-0003-3390-1932","observed_orcids":["https://orcid.org/0000-0003-3390-1932"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I2802835388","display_name":"Seoul National University Hospital","ror":"https://ror.org/01z4nnt86","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388"]},{"id":"https://openalex.org/I2803058125","display_name":"Seoul National University Bundang Hospital","ror":"https://ror.org/00cb3km46","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Sang Mee Hwang","raw_affiliation_strings":["Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015618323","display_name":"Yun Ji Hong","orcid":"https://orcid.org/0000-0001-9163-5017","observed_orcids":["https://orcid.org/0000-0001-9163-5017"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I2802835388","display_name":"Seoul National University Hospital","ror":"https://ror.org/01z4nnt86","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388"]},{"id":"https://openalex.org/I2803058125","display_name":"Seoul National University Bundang Hospital","ror":"https://ror.org/00cb3km46","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Yun Ji Hong","raw_affiliation_strings":["Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea"],"raw_orcid":"https://orcid.org/0000-0001-9163-5017","affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067411233","display_name":"Taek Soo Kim","orcid":"https://orcid.org/0000-0002-2093-1721","observed_orcids":["https://orcid.org/0000-0002-2093-1721"]},"institutions":[{"id":"https://openalex.org/I2803058125","display_name":"Seoul National University Bundang Hospital","ror":"https://ror.org/00cb3km46","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Taek Soo Kim","raw_affiliation_strings":["Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea"],"raw_orcid":"https://orcid.org/0000-0002-2093-1721","affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea","institution_ids":["https://openalex.org/I2803058125"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001540306","display_name":"Kyoung Un Park","orcid":"https://orcid.org/0000-0002-2402-7633","observed_orcids":["https://orcid.org/0000-0002-2402-7633"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I2802835388","display_name":"Seoul National University Hospital","ror":"https://ror.org/01z4nnt86","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388"]},{"id":"https://openalex.org/I2803058125","display_name":"Seoul National University Bundang Hospital","ror":"https://ror.org/00cb3km46","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Kyoung Un Park","raw_affiliation_strings":["Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. Electronic address: m91w95pf@snu.ac.kr"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. Electronic address: m91w95pf@snu.ac.kr","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023982303","display_name":"Junghan Song","orcid":"https://orcid.org/0000-0003-0576-9938","observed_orcids":["https://orcid.org/0000-0003-0576-9938"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I2802835388","display_name":"Seoul National University Hospital","ror":"https://ror.org/01z4nnt86","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388"]},{"id":"https://openalex.org/I2803058125","display_name":"Seoul National University Bundang Hospital","ror":"https://ror.org/00cb3km46","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Junghan Song","raw_affiliation_strings":["Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Bundang Seoul National University Hospital, Gyeonggi-do, Korea; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112241621","display_name":"Eui Chong Kim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Eui-Chong Kim","raw_affiliation_strings":["Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea","institution_ids":["https://openalex.org/I139264467"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5001540306"],"corresponding_institution_ids":["https://openalex.org/I139264467","https://openalex.org/I2802835388","https://openalex.org/I2803058125"],"apc_list":{"value":3550,"currency":"USD","value_usd":3550},"apc_paid":null,"fwci":2.9643,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.88881251,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"460","issue":null,"first_page":"190","last_page":"195"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9833999872207642,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9833999872207642,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11316","display_name":"Mycobacterium research and diagnosis","score":0.014700000174343586,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mycobacterial-identification","display_name":"mycobacterial identification","score":0.7990000247955322},{"id":"https://openalex.org/keywords/protein-extraction-protocol","display_name":"protein extraction protocol","score":0.671999990940094},{"id":"https://openalex.org/keywords/maldi-tof-mass-spectrometry","display_name":"MALDI-TOF mass spectrometry","score":0.9459999799728394},{"id":"https://openalex.org/keywords/maldi-biotyper","display_name":"MALDI Biotyper","score":0.9229999780654907},{"id":"https://openalex.org/keywords/vitek-ms","display_name":"VITEK MS","score":0.9480000138282776},{"id":"https://openalex.org/keywords/mycobacterium-tuberculosis-complex","display_name":"Mycobacterium tuberculosis complex","score":0.9810000061988831},{"id":"https://openalex.org/keywords/mycobacterium-avium","display_name":"Mycobacterium avium","score":0.9340000152587891},{"id":"https://openalex.org/keywords/mycobacterium-intracellulare","display_name":"Mycobacterium intracellulare","score":0.9869999885559082},{"id":"https://openalex.org/keywords/mycobacterium-abscessus","display_name":"Mycobacterium abscessus","score":0.996999979019165},{"id":"https://openalex.org/keywords/mycobacterium-fortuitum","display_name":"Mycobacterium fortuitum","score":0.9660000205039978},{"id":"https://openalex.org/keywords/clinical-isolates","display_name":"clinical isolates","score":0.625}],"concepts":[{"id":"https://openalex.org/C2780374374","wikidata":"https://www.wikidata.org/wiki/Q194309","display_name":"Mycobacterium","level":3,"score":0.6652997136116028},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5455453395843506},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.5048776268959045},{"id":"https://openalex.org/C2776111603","wikidata":"https://www.wikidata.org/wiki/Q6946978","display_name":"Mycobacterium abscessus","level":4,"score":0.4740281403064728},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4600723683834076},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3942360281944275},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.32266104221343994},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.31799376010894775},{"id":"https://openalex.org/C2781069245","wikidata":"https://www.wikidata.org/wiki/Q12204","display_name":"Tuberculosis","level":2,"score":0.2345026731491089},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.1339605152606964}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009161","descriptor_name":"Mycobacterium","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D009161","descriptor_name":"Mycobacterium","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D012107","descriptor_name":"Research Design","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":true},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.cca.2016.06.039","doi":"https://doi.org/10.1016/j.cca.2016.06.039","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.cca.2016.06.039","pdf_url":null,"source":{"id":"https://openalex.org/S207549998","display_name":"Clinica Chimica Acta","issn_l":"0009-8981","issn":["0009-8981","1873-3492"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinica Chimica Acta","raw_type":"journal-article"},{"id":"pmid:27380997","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27380997","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinica chimica acta; international journal of clinical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W3610929","https://openalex.org/W1561387207","https://openalex.org/W1666777872","https://openalex.org/W1915819047","https://openalex.org/W1953447858","https://openalex.org/W1965366579","https://openalex.org/W1989173936","https://openalex.org/W2015850250","https://openalex.org/W2042461516","https://openalex.org/W2057617333","https://openalex.org/W2067358939","https://openalex.org/W2077139574","https://openalex.org/W2096640593","https://openalex.org/W2096902831","https://openalex.org/W2097076431","https://openalex.org/W2101824041","https://openalex.org/W2102592186","https://openalex.org/W2107233349","https://openalex.org/W2124021261","https://openalex.org/W2130500685","https://openalex.org/W2139577608","https://openalex.org/W2142447693","https://openalex.org/W2150068612","https://openalex.org/W2163729956","https://openalex.org/W2172112723","https://openalex.org/W2404455782","https://openalex.org/W2461048033","https://openalex.org/W2738938338","https://openalex.org/W4243644949"],"related_works":["https://openalex.org/W2789606185","https://openalex.org/W7171835519","https://openalex.org/W2102592186","https://openalex.org/W4403273670","https://openalex.org/W2889920245","https://openalex.org/W88742442","https://openalex.org/W4382724239","https://openalex.org/W4408472273","https://openalex.org/W4224979073","https://openalex.org/W4205746730"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":6},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":6}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-07-22T00:00:00"}