{"id":"https://openalex.org/W2467429955","doi":"https://doi.org/10.1002/slct.201600515","title":"Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis","display_name":"Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis","publication_year":2016,"publication_date":"2016-07-01","ids":{"openalex":"https://openalex.org/W2467429955","doi":"https://doi.org/10.1002/slct.201600515","mag":"2467429955"},"language":"en","primary_location":{"id":"doi:10.1002/slct.201600515","doi":"https://doi.org/10.1002/slct.201600515","is_oa":true,"landing_page_url":"https://doi.org/10.1002/slct.201600515","pdf_url":"https://discovery.ucl.ac.uk/1524199/1/ward_Metagenomics%20paper%20for%20RPS.pdf","source":{"id":"https://openalex.org/S4210221416","display_name":"ChemistrySelect","issn_l":"2365-6549","issn":["2365-6549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemistrySelect","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://discovery.ucl.ac.uk/1524199/1/ward_Metagenomics%20paper%20for%20RPS.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027890644","display_name":"Jack W. E. Jeffries","orcid":"https://orcid.org/0000-0003-4143-2339","observed_orcids":["https://orcid.org/0000-0003-4143-2339"]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jack W. E. Jeffries","raw_affiliation_strings":["Department of Biochemical Engineering, University College London, Bernard Katz Building, Gordon Street, London, WC1H 0AH UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemical Engineering, University College London, Bernard Katz Building, Gordon Street, London, WC1H 0AH UK","institution_ids":["https://openalex.org/I45129253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113968067","display_name":"Natalie L. Dawson","orcid":"https://orcid.org/0000-0002-6607-9976","observed_orcids":["https://orcid.org/0000-0002-6607-9976"]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Natalie Dawson","raw_affiliation_strings":["Department of Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT UK","institution_ids":["https://openalex.org/I45129253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081250379","display_name":"Christine Anne Orengo","orcid":"https://orcid.org/0000-0002-7141-8936","observed_orcids":["https://orcid.org/0000-0002-7141-8936"]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Christine Orengo","raw_affiliation_strings":["Department of Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT UK","institution_ids":["https://openalex.org/I45129253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078486903","display_name":"Thomas S. Moody","orcid":"https://orcid.org/0000-0002-8266-0269","observed_orcids":["https://orcid.org/0000-0002-8266-0269"]},"institutions":[{"id":"https://openalex.org/I4210137931","display_name":"Almac (United Kingdom)","ror":"https://ror.org/03prym056","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210137931"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Thomas S. Moody","raw_affiliation_strings":["Department of Biocatalysis and Isotope Chemistry, Almac, 20 Seagoe Industrial Estate, Craigavon, Northern Ireland (UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biocatalysis and Isotope Chemistry, Almac, 20 Seagoe Industrial Estate, Craigavon, Northern Ireland (UK","institution_ids":["https://openalex.org/I4210137931"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111939859","display_name":"Derek J. Quinn","orcid":"https://orcid.org/0009-0003-2106-1988","observed_orcids":["https://orcid.org/0009-0003-2106-1988"]},"institutions":[{"id":"https://openalex.org/I4210137931","display_name":"Almac (United Kingdom)","ror":"https://ror.org/03prym056","country_code":"GB","type":"company","lineage":["https://openalex.org/I4210137931"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Derek J. Quinn","raw_affiliation_strings":["Department of Biocatalysis and Isotope Chemistry, Almac, 20 Seagoe Industrial Estate, Craigavon, Northern Ireland (UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biocatalysis and Isotope Chemistry, Almac, 20 Seagoe Industrial Estate, Craigavon, Northern Ireland (UK","institution_ids":["https://openalex.org/I4210137931"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5125923945","display_name":"Helen Claire Hailes","orcid":"https://orcid.org/0000-0001-5574-4742","observed_orcids":["https://orcid.org/0000-0001-5574-4742"]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Helen C. Hailes","raw_affiliation_strings":["Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ UK","institution_ids":["https://openalex.org/I45129253"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018440823","display_name":"John Maxim Ward","orcid":"https://orcid.org/0000-0002-4415-5544","observed_orcids":["https://orcid.org/0000-0002-4415-5544","https://orcid.org/0000-0001-8185-0434"]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"John M. Ward","raw_affiliation_strings":["Department of Biochemical Engineering, University College London, Bernard Katz Building, Gordon Street, London, WC1H 0AH UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemical Engineering, University College London, Bernard Katz Building, Gordon Street, London, WC1H 0AH UK","institution_ids":["https://openalex.org/I45129253"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5018440823"],"corresponding_institution_ids":["https://openalex.org/I45129253"],"apc_list":{"value":3880,"currency":"USD","value_usd":3880},"apc_paid":null,"fwci":0.7859,"has_fulltext":true,"cited_by_count":23,"citation_normalized_percentile":{"value":0.67342305,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"1","issue":"10","first_page":"2217","last_page":"2220"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9110999703407288,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9110999703407288,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.06440000236034393,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.015300000086426735,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/metagenome-mining","display_name":"metagenome mining","score":0.9269999861717224},{"id":"https://openalex.org/keywords/enzyme-discovery","display_name":"enzyme discovery","score":0.8159999847412109},{"id":"https://openalex.org/keywords/biocatalysis","display_name":"biocatalysis","score":0.9399999976158142},{"id":"https://openalex.org/keywords/open-reading-frames","display_name":"open reading frames","score":0.7599999904632568},{"id":"https://openalex.org/keywords/operon","display_name":"operon","score":0.765999972820282},{"id":"https://openalex.org/keywords/lactate-dehydrogenase","display_name":"lactate dehydrogenase","score":0.9210000038146973},{"id":"https://openalex.org/keywords/malate-dehydrogenase","display_name":"malate dehydrogenase","score":1.0},{"id":"https://openalex.org/keywords/transketolase","display_name":"transketolase","score":0.9549999833106995}],"concepts":[{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.9424218535423279},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.8120148777961731},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6855392456054688},{"id":"https://openalex.org/C59582021","wikidata":"https://www.wikidata.org/wiki/Q1128751","display_name":"Contig","level":4,"score":0.6590194702148438},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4737875163555145},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.4591829478740692},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3408414125442505},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.3217852711677551},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.27299219369888306},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.21230679750442505}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/slct.201600515","doi":"https://doi.org/10.1002/slct.201600515","is_oa":true,"landing_page_url":"https://doi.org/10.1002/slct.201600515","pdf_url":"https://discovery.ucl.ac.uk/1524199/1/ward_Metagenomics%20paper%20for%20RPS.pdf","source":{"id":"https://openalex.org/S4210221416","display_name":"ChemistrySelect","issn_l":"2365-6549","issn":["2365-6549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemistrySelect","raw_type":"journal-article"},{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:1524199","doi":null,"is_oa":false,"landing_page_url":"https://discovery.ucl.ac.uk/id/eprint/1524199/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ChemistrySelect , 1 (10) pp. 2217-2220. (2016)","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1002/slct.201600515","doi":"https://doi.org/10.1002/slct.201600515","is_oa":true,"landing_page_url":"https://doi.org/10.1002/slct.201600515","pdf_url":"https://discovery.ucl.ac.uk/1524199/1/ward_Metagenomics%20paper%20for%20RPS.pdf","source":{"id":"https://openalex.org/S4210221416","display_name":"ChemistrySelect","issn_l":"2365-6549","issn":["2365-6549"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemistrySelect","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7696377006","display_name":null,"funder_award_id":"BB/L010801/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G8904207333","display_name":"An Greatly Expanded CATH-Gene3D with Functional Fingerprints to Characterise Proteins","funder_award_id":"BB/K020013/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2467429955.pdf","grobid_xml":"https://content.openalex.org/works/W2467429955.grobid-xml"},"referenced_works_count":31,"referenced_works":["https://openalex.org/W1542883299","https://openalex.org/W1652052270","https://openalex.org/W1932191483","https://openalex.org/W1968042342","https://openalex.org/W1968173884","https://openalex.org/W1968567821","https://openalex.org/W1968592931","https://openalex.org/W1976236461","https://openalex.org/W1984180361","https://openalex.org/W1989179901","https://openalex.org/W1992635639","https://openalex.org/W1993723070","https://openalex.org/W1996729036","https://openalex.org/W2021457453","https://openalex.org/W2025294451","https://openalex.org/W2026006287","https://openalex.org/W2026352085","https://openalex.org/W2036389069","https://openalex.org/W2038151471","https://openalex.org/W2055777398","https://openalex.org/W2055934341","https://openalex.org/W2057120602","https://openalex.org/W2066381187","https://openalex.org/W2070357510","https://openalex.org/W2075716829","https://openalex.org/W2085242402","https://openalex.org/W2111877772","https://openalex.org/W2124043771","https://openalex.org/W2147783737","https://openalex.org/W2156387173","https://openalex.org/W2169772650"],"related_works":["https://openalex.org/W2762180220","https://openalex.org/W1508934482","https://openalex.org/W3183241114","https://openalex.org/W4206942985","https://openalex.org/W4392924074","https://openalex.org/W7222902191","https://openalex.org/W4241874137","https://openalex.org/W4297131566","https://openalex.org/W3156493549","https://openalex.org/W2904345037"],"abstract_inverted_index":{"Abstract":[0],"Biocatalytic":[1],"reactions":[2],"are":[3],"increasingly":[4],"being":[5],"used":[6,46],"as":[7],"a":[8,33,66,85,88],"sustainable":[9],"strategy":[10],"in":[11,50,95],"organic":[12],"synthesis":[13],"and":[14,30,45,72,91,100,115],"it":[15],"is":[16,20],"recognised":[17],"that":[18],"there":[19],"need":[21],"for":[22],"new":[23,103],"enzyme":[24,104],"discovery.":[25],"To":[26],"establish":[27],"the":[28,40,63,76,119],"utility":[29],"versatility":[31],"of":[32,65,83],"metagenomics":[34],"approach,":[35],"metagenomic":[36,113,127],"DNA":[37,78],"extracted":[38],"from":[39,75],"oral":[41],"cavity":[42],"was":[43],"sequenced":[44],"to":[47,69,112],"create":[48],"an":[49],"silico":[51,96],"contig":[52],"library.":[53],"This":[54,102],"enables":[55],"individual":[56],"open":[57],"reading":[58],"frames,":[59],"operons":[60],"or":[61],"all":[62],"enzymes":[64],"particular":[67],"family":[68],"be":[70],"identified":[71,94],"then":[73],"retrieved":[74],"original":[77],"by":[79],"PCR.":[80],"As":[81],"proof":[82],"principle":[84],"lactate":[86],"dehydrogenase,":[87],"malate":[89],"dehydrogenase":[90],"transketolases":[92],"were":[93],",":[97],"successfully":[98],"cloned":[99],"assayed.":[101],"retrieval":[105],"sequence":[106],"directed":[107],"method":[108],"gives":[109],"constructive":[110],"access":[111],"diversity":[114],"importantly":[116],"improves":[117],"on":[118],"low":[120],"hit":[121],"rate":[122],"experienced":[123],"when":[124],"using":[125],"conventional":[126],"screens.":[128]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-07-22T00:00:00"}