{"id":"https://openalex.org/W2465664700","doi":"https://doi.org/10.3390/metabo6030021","title":"Decoding Biosynthetic Pathways in Plants by Pulse-Chase Strategies Using 13CO2 as a Universal Tracer","display_name":"Decoding Biosynthetic Pathways in Plants by Pulse-Chase Strategies Using 13CO2 as a Universal Tracer","publication_year":2016,"publication_date":"2016-07-14","ids":{"openalex":"https://openalex.org/W2465664700","doi":"https://doi.org/10.3390/metabo6030021","mag":"2465664700","pmid":"https://pubmed.ncbi.nlm.nih.gov/27429012","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5041120"},"language":"en","primary_location":{"id":"doi:10.3390/metabo6030021","doi":"https://doi.org/10.3390/metabo6030021","is_oa":true,"landing_page_url":"https://doi.org/10.3390/metabo6030021","pdf_url":"https://www.mdpi.com/2218-1989/6/3/21/pdf?version=1468475940","source":{"id":"https://openalex.org/S2764402194","display_name":"Metabolites","issn_l":"2218-1989","issn":["2218-1989"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolites","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2218-1989/6/3/21/pdf?version=1468475940","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068798153","display_name":"Adelbert Bacher","orcid":"https://orcid.org/0000-0001-5174-4139","observed_orcids":["https://orcid.org/0000-0001-5174-4139"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Adelbert Bacher","raw_affiliation_strings":["Lehrstuhl f\u00fcr Biochemie, Technische Universit\u00e4t M\u00fcnchen, 85748 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Biochemie, Technische Universit\u00e4t M\u00fcnchen, 85748 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Fan Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Fan Chen","raw_affiliation_strings":["Lehrstuhl f\u00fcr Biochemie, Technische Universit\u00e4t M\u00fcnchen, 85748 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Biochemie, Technische Universit\u00e4t M\u00fcnchen, 85748 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086504004","display_name":"Wolfgang Eisenreich","orcid":"https://orcid.org/0000-0002-9832-8279","observed_orcids":["https://orcid.org/0000-0002-9832-8279"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Wolfgang Eisenreich","raw_affiliation_strings":["Lehrstuhl f\u00fcr Biochemie, Technische Universit\u00e4t M\u00fcnchen, 85748 Garching, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Biochemie, Technische Universit\u00e4t M\u00fcnchen, 85748 Garching, Germany","institution_ids":["https://openalex.org/I62916508"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5086504004"],"corresponding_institution_ids":["https://openalex.org/I62916508"],"apc_list":{"value":2700,"currency":"CHF","value_usd":3258},"apc_paid":{"value":656,"currency":"EUR","value_usd":707},"fwci":0.151,"has_fulltext":true,"cited_by_count":21,"citation_normalized_percentile":{"value":0.1068142,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"6","issue":"3","first_page":"21","last_page":"21"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9721999764442444,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9721999764442444,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.012299999594688416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.005200000014156103,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/13c-labeling","display_name":"13C labeling","score":0.5189999938011169},{"id":"https://openalex.org/keywords/skeletal-rearrangement","display_name":"skeletal rearrangement","score":0.6069999933242798},{"id":"https://openalex.org/keywords/field-grown-plants","display_name":"field-grown plants","score":0.6100000143051147}],"concepts":[{"id":"https://openalex.org/C13934619","wikidata":"https://www.wikidata.org/wiki/Q2198720","display_name":"Isotopologue","level":3,"score":0.9704984426498413},{"id":"https://openalex.org/C2778863792","wikidata":"https://www.wikidata.org/wiki/Q16909918","display_name":"TRACER","level":2,"score":0.6816388368606567},{"id":"https://openalex.org/C57273362","wikidata":"https://www.wikidata.org/wiki/Q576722","display_name":"Decoding methods","level":2,"score":0.6215500831604004},{"id":"https://openalex.org/C2780167933","wikidata":"https://www.wikidata.org/wiki/Q1550652","display_name":"Pulse (music)","level":3,"score":0.4960828721523285},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.487265944480896},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.4474659562110901},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.44369152188301086},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.43371349573135376},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4288107454776764},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.40792614221572876},{"id":"https://openalex.org/C184720557","wikidata":"https://www.wikidata.org/wiki/Q7825049","display_name":"Topology (electrical circuits)","level":2,"score":0.39015835523605347},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3567647933959961},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.341346800327301},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.3295097053050995},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26669996976852417},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.2613280117511749},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22662699222564697},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.2198861837387085},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.19088497757911682},{"id":"https://openalex.org/C107872376","wikidata":"https://www.wikidata.org/wiki/Q321355","display_name":"Environmental chemistry","level":1,"score":0.17768070101737976},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.16447675228118896},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.11647868156433105},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.10764467716217041},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.0},{"id":"https://openalex.org/C94915269","wikidata":"https://www.wikidata.org/wiki/Q1834857","display_name":"Detector","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.3390/metabo6030021","doi":"https://doi.org/10.3390/metabo6030021","is_oa":true,"landing_page_url":"https://doi.org/10.3390/metabo6030021","pdf_url":"https://www.mdpi.com/2218-1989/6/3/21/pdf?version=1468475940","source":{"id":"https://openalex.org/S2764402194","display_name":"Metabolites","issn_l":"2218-1989","issn":["2218-1989"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolites","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:4008833","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5041120","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5041120","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5041120","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolites","raw_type":"Text"},{"id":"pmid:27429012","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27429012","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolites","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:20b2c08704ba46cd8f6897bf85e5dc3b","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/20b2c08704ba46cd8f6897bf85e5dc3b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Metabolites, Vol 6, Iss 3, p 21 (2016)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/metabo6030021","doi":"https://doi.org/10.3390/metabo6030021","is_oa":true,"landing_page_url":"https://doi.org/10.3390/metabo6030021","pdf_url":"https://www.mdpi.com/2218-1989/6/3/21/pdf?version=1468475940","source":{"id":"https://openalex.org/S2764402194","display_name":"Metabolites","issn_l":"2218-1989","issn":["2218-1989"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Metabolites","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2465664700.pdf","grobid_xml":"https://content.openalex.org/works/W2465664700.grobid-xml"},"referenced_works_count":56,"referenced_works":["https://openalex.org/W108684213","https://openalex.org/W1501419762","https://openalex.org/W1767787079","https://openalex.org/W1834965185","https://openalex.org/W1912527875","https://openalex.org/W1934935301","https://openalex.org/W1969658291","https://openalex.org/W1972430667","https://openalex.org/W1975113414","https://openalex.org/W1975905816","https://openalex.org/W1978528260","https://openalex.org/W1983989854","https://openalex.org/W1997679574","https://openalex.org/W2004835566","https://openalex.org/W2008374136","https://openalex.org/W2020004064","https://openalex.org/W2020942089","https://openalex.org/W2021392041","https://openalex.org/W2024975833","https://openalex.org/W2026254413","https://openalex.org/W2028769741","https://openalex.org/W2036479712","https://openalex.org/W2037985940","https://openalex.org/W2044066157","https://openalex.org/W2057239740","https://openalex.org/W2058467542","https://openalex.org/W2062382198","https://openalex.org/W2069226156","https://openalex.org/W2077541178","https://openalex.org/W2080464145","https://openalex.org/W2091967608","https://openalex.org/W2092320142","https://openalex.org/W2093237759","https://openalex.org/W2093396903","https://openalex.org/W2094404220","https://openalex.org/W2100481323","https://openalex.org/W2110256992","https://openalex.org/W2110659056","https://openalex.org/W2115965743","https://openalex.org/W2118489229","https://openalex.org/W2127225493","https://openalex.org/W2127254165","https://openalex.org/W2127543426","https://openalex.org/W2128896661","https://openalex.org/W2135091034","https://openalex.org/W2139723878","https://openalex.org/W2164727176","https://openalex.org/W2198989159","https://openalex.org/W2216069753","https://openalex.org/W2264677326","https://openalex.org/W2319897744","https://openalex.org/W2327382810","https://openalex.org/W2345421965","https://openalex.org/W2400555721","https://openalex.org/W2414189057","https://openalex.org/W3118517130"],"related_works":["https://openalex.org/W1969658291","https://openalex.org/W2050252983","https://openalex.org/W183479009","https://openalex.org/W2031669142","https://openalex.org/W2083364998","https://openalex.org/W4394993782","https://openalex.org/W2028616062","https://openalex.org/W2327382810","https://openalex.org/W4236010503","https://openalex.org/W2092320142"],"abstract_inverted_index":{"(13)CO\u2082":[0,33,59,103],"pulse-chase":[1,34],"experiments":[2,77],"monitored":[3],"by":[4,45,151,164],"high-resolution":[5],"NMR":[6],"spectroscopy":[7],"and":[8,80,111],"mass":[9],"spectrometry":[10],"can":[11,42,81,147,155],"provide":[12],"(13)C-isotopologue":[13],"compositions":[14],"in":[15,91,168,179],"biosynthetic":[16,100,187],"products.":[17],"Experiments":[18],"with":[19,32,78,102],"a":[20],"variety":[21],"of":[22,48,57,99,105,108,114,136,142,184],"plant":[23],"species":[24],"have":[25,118,148],"documented":[26],"that":[27,41,69,117,129,154],"the":[28,46,55,58,66,92,97,106,123,133,140,159,165,172,181],"isotopologue":[29,160],"profiles":[30],"generated":[31,44],"labeling":[35,60],"are":[36],"directly":[37],"comparable":[38],"to":[39,50,65,72,84,122,176],"those":[40],"be":[43,82,156,177],"application":[47,56],"[U-(13)C\u2086]glucose":[49,79],"aseptically":[51],"growing":[52,86],"plants.":[53],"However,":[54],"technology":[61],"is":[62,130],"not":[63],"subject":[64],"experimental":[67],"limitations":[68],"one":[70],"has":[71],"take":[73],"into":[74],"account":[75],"for":[76],"applied":[83],"plants":[85],"under":[87],"physiological":[88],"conditions,":[89],"even":[90,185],"field.":[93],"In":[94],"practical":[95],"terms,":[96],"results":[98],"studies":[101],"consist":[104],"detection":[107],"pairs,":[109],"triples":[110],"occasionally":[112],"quadruples":[113],"(13)C":[115,145],"atoms":[116],"been":[119],"jointly":[120,143],"contributed":[121],"target":[124],"metabolite,":[125],"at":[126],"an":[127],"abundance":[128],"well":[131],"above":[132],"stochastic":[134],"occurrence":[135],"such":[137],"multiples.":[138],"Notably,":[139],"connectivities":[141],"transferred":[144],"multiples":[146],"undergone":[149],"modification":[150],"skeletal":[152],"rearrangements":[153],"diagnosed":[157],"from":[158],"data.":[161],"As":[162],"shown":[163],"examples":[166],"presented":[167],"this":[169],"review":[170],"article,":[171],"approach":[173],"turns":[174],"out":[175],"powerful":[178],"decoding":[180],"carbon":[182],"topology":[183],"complex":[186],"pathways.":[188]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-07-22T00:00:00"}