{"id":"https://openalex.org/W2463063597","doi":"https://doi.org/10.7860/jcdr/2015/13543.6313","title":"Comparative Analysis of Urinary Total Proteins by Bicinchoninic Acid and Pyrogallol Red Molybdate Methods","display_name":"Comparative Analysis of Urinary Total Proteins by Bicinchoninic Acid and Pyrogallol Red Molybdate Methods","publication_year":2015,"publication_date":"2015-01-01","ids":{"openalex":"https://openalex.org/W2463063597","doi":"https://doi.org/10.7860/jcdr/2015/13543.6313","mag":"2463063597","pmid":"https://pubmed.ncbi.nlm.nih.gov/26435938","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4576529"},"language":"en","primary_location":{"id":"doi:10.7860/jcdr/2015/13543.6313","doi":"https://doi.org/10.7860/jcdr/2015/13543.6313","is_oa":true,"landing_page_url":"https://doi.org/10.7860/jcdr/2015/13543.6313","pdf_url":null,"source":{"id":"https://openalex.org/S2737465529","display_name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","issn_l":"0973-709X","issn":["0973-709X","2249-782X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","poland-40"],"host_organization":"https://openalex.org/P4310318655","host_organization_name":"JCDR Research and Publications Private Limited","host_organization_lineage":["https://openalex.org/P4310318655"],"host_organization_lineage_names":["JCDR Research and Publications Private Limited"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7860/jcdr/2015/13543.6313","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004676207","display_name":"Padma Yalamati","orcid":"https://orcid.org/0000-0002-8329-4766","observed_orcids":["https://orcid.org/0000-0002-8329-4766"]},"institutions":[{"id":"https://openalex.org/I3130691990","display_name":"Mediciti Institute of Medical Sciences","ror":"https://ror.org/032y8tg91","country_code":"IN","type":"education","lineage":["https://openalex.org/I3130691990"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Padma Yalamati","raw_affiliation_strings":["Associate Professor, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India "],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Associate Professor, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India ","institution_ids":["https://openalex.org/I3130691990"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083842098","display_name":"Aparna Varma Bhongir","orcid":"https://orcid.org/0000-0002-3464-271X","observed_orcids":["https://orcid.org/0000-0002-3464-271X"]},"institutions":[{"id":"https://openalex.org/I3130691990","display_name":"Mediciti Institute of Medical Sciences","ror":"https://ror.org/032y8tg91","country_code":"IN","type":"education","lineage":["https://openalex.org/I3130691990"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Aparna Varma Bhongir","raw_affiliation_strings":["Professor/HOD, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India "],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Professor/HOD, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India ","institution_ids":["https://openalex.org/I3130691990"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073477128","display_name":"Madhu Latha Karra","orcid":"https://orcid.org/0000-0002-1058-4682","observed_orcids":["https://orcid.org/0000-0002-1058-4682"]},"institutions":[{"id":"https://openalex.org/I3130691990","display_name":"Mediciti Institute of Medical Sciences","ror":"https://ror.org/032y8tg91","country_code":"IN","type":"education","lineage":["https://openalex.org/I3130691990"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Madhulatha Karra","raw_affiliation_strings":["Post Graduate, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India "],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Post Graduate, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India ","institution_ids":["https://openalex.org/I3130691990"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055920760","display_name":"Sashidhar Rao Beedu","orcid":"https://orcid.org/0000-0001-9289-0394","observed_orcids":["https://orcid.org/0000-0001-9289-0394"]},"institutions":[{"id":"https://openalex.org/I3130691990","display_name":"Mediciti Institute of Medical Sciences","ror":"https://ror.org/032y8tg91","country_code":"IN","type":"education","lineage":["https://openalex.org/I3130691990"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sashidhar Rao Beedu","raw_affiliation_strings":["Post Graduate, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India "],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Post Graduate, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India ","institution_ids":["https://openalex.org/I3130691990"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I3130691990"],"apc_list":{"value":275,"currency":"USD","value_usd":275},"apc_paid":{"value":275,"currency":"USD","value_usd":275},"fwci":4.4312,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.9270648,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"9","issue":"8","first_page":"BC01","last_page":"4"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10408","display_name":"Chronic Kidney Disease and Diabetes","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/urine-protein","display_name":"urinary protein","score":0.9679999947547913},{"id":"https://openalex.org/keywords/method-comparison","display_name":"method comparison","score":0.7929999828338623},{"id":"https://openalex.org/keywords/renal-function","display_name":"renal function","score":0.5120000243186951},{"id":"https://openalex.org/keywords/method-agreement","display_name":"measurement agreement","score":0.5889999866485596},{"id":"https://openalex.org/keywords/urine-protein-measurement","display_name":"urine protein measurement","score":0.35600000619888306}],"concepts":[{"id":"https://openalex.org/C2780026642","wikidata":"https://www.wikidata.org/wiki/Q40924","display_name":"Urine","level":2,"score":0.8967704772949219},{"id":"https://openalex.org/C72673979","wikidata":"https://www.wikidata.org/wiki/Q17003157","display_name":"Bicinchoninic acid assay","level":2,"score":0.8139287233352661},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6583998799324036},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6426922082901001},{"id":"https://openalex.org/C2776344562","wikidata":"https://www.wikidata.org/wiki/Q388692","display_name":"Pyrogallol","level":2,"score":0.621778666973114},{"id":"https://openalex.org/C77411442","wikidata":"https://www.wikidata.org/wiki/Q181100","display_name":"Urinary system","level":2,"score":0.49492448568344116},{"id":"https://openalex.org/C160845552","wikidata":"https://www.wikidata.org/wiki/Q247562","display_name":"Bradford protein assay","level":2,"score":0.4558374881744385},{"id":"https://openalex.org/C89838059","wikidata":"https://www.wikidata.org/wiki/Q623738","display_name":"Coefficient of variation","level":2,"score":0.45233675837516785},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19057834148406982},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.17900383472442627},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.10515853762626648}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.7860/jcdr/2015/13543.6313","doi":"https://doi.org/10.7860/jcdr/2015/13543.6313","is_oa":true,"landing_page_url":"https://doi.org/10.7860/jcdr/2015/13543.6313","pdf_url":null,"source":{"id":"https://openalex.org/S2737465529","display_name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","issn_l":"0973-709X","issn":["0973-709X","2249-782X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","poland-40"],"host_organization":"https://openalex.org/P4310318655","host_organization_name":"JCDR Research and Publications Private Limited","host_organization_lineage":["https://openalex.org/P4310318655"],"host_organization_lineage_names":["JCDR Research and Publications Private Limited"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3569040","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4576529","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:26435938","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26435938","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of clinical and diagnostic research : JCDR","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4576529","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4576529/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Diagn Res","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:d28c9c99b2ec400581c4c9652a88a511","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/d28c9c99b2ec400581c4c9652a88a511","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Clinical and Diagnostic Research, Vol 9, Iss 8, Pp BC01-BC04 (2015)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.7860/jcdr/2015/13543.6313","doi":"https://doi.org/10.7860/jcdr/2015/13543.6313","is_oa":true,"landing_page_url":"https://doi.org/10.7860/jcdr/2015/13543.6313","pdf_url":null,"source":{"id":"https://openalex.org/S2737465529","display_name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","issn_l":"0973-709X","issn":["0973-709X","2249-782X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","poland-40"],"host_organization":"https://openalex.org/P4310318655","host_organization_name":"JCDR Research and Publications Private Limited","host_organization_lineage":["https://openalex.org/P4310318655"],"host_organization_lineage_names":["JCDR Research and Publications Private Limited"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[{"display_name":"Observational Study","id":"https://openalex.org/study-designs/observational-study"}],"awards":[{"id":"https://openalex.org/G5990299047","display_name":"SHARE India University Pittsburgh Population and Intervention Research Training","funder_award_id":"D43TW009078","funder_id":"https://openalex.org/F4320337356","funder_display_name":"Fogarty International Center"}],"funders":[{"id":"https://openalex.org/F4320337356","display_name":"Fogarty International Center","ror":"https://ror.org/02xey9a22"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W30611175","https://openalex.org/W154168183","https://openalex.org/W1760632681","https://openalex.org/W1775749144","https://openalex.org/W1819350117","https://openalex.org/W1848564593","https://openalex.org/W1899626786","https://openalex.org/W1905217126","https://openalex.org/W1923726442","https://openalex.org/W1953604256","https://openalex.org/W2020210187","https://openalex.org/W2044485421","https://openalex.org/W2063968553","https://openalex.org/W2077481501","https://openalex.org/W2102071794","https://openalex.org/W2108434580","https://openalex.org/W2117871749","https://openalex.org/W2128635872","https://openalex.org/W2317683109","https://openalex.org/W2402348249","https://openalex.org/W2436250463","https://openalex.org/W4232534952","https://openalex.org/W4301773808"],"related_works":["https://openalex.org/W2356734109","https://openalex.org/W2348535154","https://openalex.org/W2372547809","https://openalex.org/W2125871134","https://openalex.org/W2367514276","https://openalex.org/W3036218282","https://openalex.org/W1924266466","https://openalex.org/W7097005999","https://openalex.org/W2389525488","https://openalex.org/W2005547488"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,23,115,243,271],"concentration":[2,85],"of":[3,12,39,43,89,105,117,126,170,183,195,221,245,303],"total":[4,31,128],"proteins":[5,32,129],"in":[6,37,75,86,98],"urine":[7,30,77,101,127,160,177,224,297],"is":[8,18,33,56,120,153,299],"a":[9,154,167,180],"good":[10],"index":[11],"renal":[13],"function,":[14],"but":[15],"its":[16,44,50],"determination":[17],"found":[19],"to":[20,121,135,145,301,311],"be":[21,140],"unreliable.":[22],"pyrogallol":[24],"red":[25],"molybdate":[26],"(PRM)":[27],"method":[28,54,70,74,80,107,132,137,253,264],"for":[29,59,250,261],"being":[34],"widely":[35],"used":[36,58,141],"most":[38],"the":[40,72,82,87,94,99,103,111,118,124,146,163,202,219,222,228,251,262,293,306],"hospitals":[41],"because":[42],"high":[45],"sensitivity,":[46],"better":[47],"precision":[48],"and":[49,62,138,192,213,234,236,238,247,256,260,267,288],"practicability.":[51],"Bicinchoninic":[52],"acid":[53],"(BCA)":[55],"also":[57],"protein":[60,84,171,184],"estimation":[61],"there":[63],"have":[64],"been":[65],"no":[66,285],"studies":[67,283],"comparing":[68,218],"this":[69],"with":[71,110],"PRM":[73,112,136,147,263,312],"human":[76,100],"samples.":[78],"BCA":[79,106,131,252,307],"overestimates":[81],"urinary":[83],"presence":[88],"interfering":[90,95,304],"substances.":[91],"After":[92],"removing":[93],"substances":[96],"present":[97],"samples":[102,225],"results":[104,125,220,308],"were":[108,198,254,265],"compared":[109],"method.":[113,148,313],"AIM:":[114],"purpose":[116],"study":[119,156,212],"identify":[122],"whether":[123],"by":[130,200,209,217,226],"are":[133,309],"comparable":[134,310],"can":[139],"as":[142],"an":[143],"alternative":[144],"SETTING":[149],"AND":[150,174],"DESIGN:":[151],"This":[152],"cross-sectional":[155],"done":[157],"on":[158],"fresh":[159],"specimens":[161,178],"from":[162],"hospital":[164],"laboratory,":[165],"covering":[166,179],"wide":[168,181],"range":[169,182],"concentrations.":[172],"MATERIAL":[173],"METHODS:":[175],"Fresh":[176],"concentrations":[185],"(urine":[186],"dipstick:":[187],"nil,":[188],"trace,":[189],"1+,":[190],"2+":[191],"\u2265":[193],"3+)":[194],"36":[196],"patients":[197],"analysed":[199],"both":[201,227,292],"methods.":[203,294],"STATISTICAL":[204],"ANALYSIS:":[205],"Imprecision":[206],"was":[207,215,276],"determined":[208],"repeated":[210],"analysis":[211],"Inaccuracy":[214],"assessed":[216],"patient's":[223],"methods":[229],"using":[230],"correlation":[231,273],"plots,":[232],"Bland":[233],"Altman,":[235],"Passing":[237],"Bablok":[239],"regression":[240],"analyses.":[241],"RESULTS:":[242],"coefficient":[244],"variation":[246],"mean":[248],"(SD)":[249],"4.6%":[255],"799.1":[257],"(882.5)":[258],"mg/L":[259],"5.1%":[266],"802.1":[268],"(911.9)":[269],"mg/L.":[270],"Pearson":[272],"coefficient,":[274],"r":[275],"0.93":[277],"(p":[278],"<":[279],"0.0001).":[280],"Method":[281],"agreement":[282],"showed":[284],"significant":[286],"constant":[287],"proportional":[289],"bias":[290],"between":[291],"CONCLUSION:":[295],"In":[296],"which":[298],"subjected":[300],"removal":[302],"substances,":[305]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-07-22T00:00:00"}