{"id":"https://openalex.org/W2462480965","doi":"https://doi.org/10.5897/ajmr2016.8083","title":"Characterization and purification of bacteriocin produced by Enterococcus sp. GHB26 isolated from Algerian paste of dates Ghars","display_name":"Characterization and purification of bacteriocin produced by Enterococcus sp. GHB26 isolated from Algerian paste of dates Ghars","publication_year":2016,"publication_date":"2016-07-07","ids":{"openalex":"https://openalex.org/W2462480965","doi":"https://doi.org/10.5897/ajmr2016.8083","mag":"2462480965"},"language":"en","primary_location":{"id":"doi:10.5897/ajmr2016.8083","doi":"https://doi.org/10.5897/ajmr2016.8083","is_oa":true,"landing_page_url":"https://doi.org/10.5897/ajmr2016.8083","pdf_url":"https://academicjournals.org/journal/AJMR/article-full-text-pdf/5010E7059288","source":{"id":"https://openalex.org/S109711878","display_name":"African Journal of Microbiology Research","issn_l":"1996-0808","issn":["1996-0808"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310321074","host_organization_name":"Academic Journals","host_organization_lineage":["https://openalex.org/P4310321074"],"host_organization_lineage_names":["Academic Journals"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"African Journal of Microbiology Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://academicjournals.org/journal/AJMR/article-full-text-pdf/5010E7059288","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5097969437","display_name":"Kout Amel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137407579","display_name":"Universit\u00e9 Oran 1 Ahmed Ben Bella","ror":"https://ror.org/059et2b68","country_code":"DZ","type":"education","lineage":["https://openalex.org/I137407579"]}],"countries":["DZ"],"is_corresponding":true,"raw_author_name":"Kout Amel","raw_affiliation_strings":["Ahmed Ben Bella , B. P. 1524 , El M'Naouer , 31000 Oran , Algeria","Laboratory of Biology of Microorganisms and Biotechnology , University of Oran","Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ahmed Ben Bella , B. P. 1524 , El M'Naouer , 31000 Oran , Algeria","institution_ids":["https://openalex.org/I137407579"]},{"raw_affiliation_string":"Laboratory of Biology of Microorganisms and Biotechnology , University of Oran","institution_ids":["https://openalex.org/I137407579"]},{"raw_affiliation_string":"Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria","institution_ids":["https://openalex.org/I137407579"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088768003","display_name":"Fatiha Dalache","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137407579","display_name":"Universit\u00e9 Oran 1 Ahmed Ben Bella","ror":"https://ror.org/059et2b68","country_code":"DZ","type":"education","lineage":["https://openalex.org/I137407579"]},{"id":"https://openalex.org/I177657909","display_name":"Universit\u00e9 de Mostaganem","ror":"https://ror.org/052r04325","country_code":"DZ","type":"education","lineage":["https://openalex.org/I177657909"]}],"countries":["DZ"],"is_corresponding":false,"raw_author_name":"Dalache Fatiha","raw_affiliation_strings":["Biology Department, Faculty of Natural Sciences and Life, University of Abdelhamid Ibn Badis of Mostaganem, B. P. 188, 27000 Mostaganem, Algeria","Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Department, Faculty of Natural Sciences and Life, University of Abdelhamid Ibn Badis of Mostaganem, B. P. 188, 27000 Mostaganem, Algeria","institution_ids":["https://openalex.org/I177657909"]},{"raw_affiliation_string":"Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria","institution_ids":["https://openalex.org/I137407579"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112503948","display_name":"Halima Zadi-Karam","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137407579","display_name":"Universit\u00e9 Oran 1 Ahmed Ben Bella","ror":"https://ror.org/059et2b68","country_code":"DZ","type":"education","lineage":["https://openalex.org/I137407579"]}],"countries":["DZ"],"is_corresponding":false,"raw_author_name":"Zadi Karam Halima","raw_affiliation_strings":["Ahmed Ben Bella , B. P. 1524 , El M'Naouer , 31000 Oran , Algeria","Laboratory of Biology of Microorganisms and Biotechnology , University of Oran","Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ahmed Ben Bella , B. P. 1524 , El M'Naouer , 31000 Oran , Algeria","institution_ids":["https://openalex.org/I137407579"]},{"raw_affiliation_string":"Laboratory of Biology of Microorganisms and Biotechnology , University of Oran","institution_ids":["https://openalex.org/I137407579"]},{"raw_affiliation_string":"Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria","institution_ids":["https://openalex.org/I137407579"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5106342095","display_name":"Karam Nour-Eddine","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137407579","display_name":"Universit\u00e9 Oran 1 Ahmed Ben Bella","ror":"https://ror.org/059et2b68","country_code":"DZ","type":"education","lineage":["https://openalex.org/I137407579"]}],"countries":["DZ"],"is_corresponding":false,"raw_author_name":"Karam Nour Eddine","raw_affiliation_strings":["Ahmed Ben Bella , B. P. 1524 , El M'Naouer , 31000 Oran , Algeria","Laboratory of Biology of Microorganisms and Biotechnology , University of Oran","Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ahmed Ben Bella , B. P. 1524 , El M'Naouer , 31000 Oran , Algeria","institution_ids":["https://openalex.org/I137407579"]},{"raw_affiliation_string":"Laboratory of Biology of Microorganisms and Biotechnology , University of Oran","institution_ids":["https://openalex.org/I137407579"]},{"raw_affiliation_string":"Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M'Naouer, 31000 Oran, Algeria","institution_ids":["https://openalex.org/I137407579"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5097969437"],"corresponding_institution_ids":["https://openalex.org/I137407579"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.06709393,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"10","issue":"25","first_page":"930","last_page":"937"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.006099999882280827,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12894","display_name":"Date Palm Research Studies","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacteriocins","display_name":"bacteriocins","score":0.9950000047683716},{"id":"https://openalex.org/keywords/enterococcus","display_name":"Enterococcus","score":0.9369999766349792},{"id":"https://openalex.org/keywords/antibacterial-activity","display_name":"antibacterial activity","score":0.8410000205039978},{"id":"https://openalex.org/keywords/bacteriocin-purification","display_name":"bacteriocin purification","score":0.9449999928474426},{"id":"https://openalex.org/keywords/protease-inactivation","display_name":"protease inactivation","score":0.6110000014305115},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.5690000057220459},{"id":"https://openalex.org/keywords/chemical-stability","display_name":"chemical stability","score":0.5580000281333923}],"concepts":[{"id":"https://openalex.org/C115574571","wikidata":"https://www.wikidata.org/wiki/Q183119","display_name":"Bacteriocin","level":3,"score":0.9405291080474854},{"id":"https://openalex.org/C163994747","wikidata":"https://www.wikidata.org/wiki/Q2916358","display_name":"Sephadex","level":3,"score":0.7260532379150391},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7236042618751526},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.695371150970459},{"id":"https://openalex.org/C2776428949","wikidata":"https://www.wikidata.org/wiki/Q18814659","display_name":"Ammonium sulfate precipitation","level":4,"score":0.673539400100708},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.5930445790290833},{"id":"https://openalex.org/C51724486","wikidata":"https://www.wikidata.org/wiki/Q908955","display_name":"Column chromatography","level":2,"score":0.5212967395782471},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.4237607419490814},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24064823985099792},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.12941721081733704},{"id":"https://openalex.org/C4937899","wikidata":"https://www.wikidata.org/wiki/Q68541106","display_name":"Antimicrobial","level":2,"score":0.07493272423744202},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.061614930629730225}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.5897/ajmr2016.8083","doi":"https://doi.org/10.5897/ajmr2016.8083","is_oa":true,"landing_page_url":"https://doi.org/10.5897/ajmr2016.8083","pdf_url":"https://academicjournals.org/journal/AJMR/article-full-text-pdf/5010E7059288","source":{"id":"https://openalex.org/S109711878","display_name":"African Journal of Microbiology Research","issn_l":"1996-0808","issn":["1996-0808"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310321074","host_organization_name":"Academic Journals","host_organization_lineage":["https://openalex.org/P4310321074"],"host_organization_lineage_names":["Academic Journals"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"African Journal of Microbiology Research","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.5897/ajmr2016.8083","doi":"https://doi.org/10.5897/ajmr2016.8083","is_oa":true,"landing_page_url":"https://doi.org/10.5897/ajmr2016.8083","pdf_url":"https://academicjournals.org/journal/AJMR/article-full-text-pdf/5010E7059288","source":{"id":"https://openalex.org/S109711878","display_name":"African Journal of Microbiology Research","issn_l":"1996-0808","issn":["1996-0808"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310321074","host_organization_name":"Academic Journals","host_organization_lineage":["https://openalex.org/P4310321074"],"host_organization_lineage_names":["Academic Journals"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"African Journal of Microbiology Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G619548971","display_name":null,"funder_award_id":"012/2000","funder_id":"https://openalex.org/F4320335007","funder_display_name":"Direction G\u00e9n\u00e9rale de la Recherche Scientifique et du D\u00e9veloppement Technologique"}],"funders":[{"id":"https://openalex.org/F4320335007","display_name":"Direction G\u00e9n\u00e9rale de la Recherche Scientifique et du D\u00e9veloppement Technologique","ror":"https://ror.org/04jpfrv51"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2462480965.pdf","grobid_xml":"https://content.openalex.org/works/W2462480965.grobid-xml"},"referenced_works_count":31,"referenced_works":["https://openalex.org/W207183365","https://openalex.org/W1518196898","https://openalex.org/W1949136023","https://openalex.org/W1979963523","https://openalex.org/W2018019550","https://openalex.org/W2019289226","https://openalex.org/W2025494790","https://openalex.org/W2033722341","https://openalex.org/W2047926370","https://openalex.org/W2052833935","https://openalex.org/W2053935361","https://openalex.org/W2055155126","https://openalex.org/W2062716714","https://openalex.org/W2064870983","https://openalex.org/W2079629926","https://openalex.org/W2091588914","https://openalex.org/W2118493043","https://openalex.org/W2135213452","https://openalex.org/W2141116096","https://openalex.org/W2141166131","https://openalex.org/W2143266810","https://openalex.org/W2144633410","https://openalex.org/W2147822305","https://openalex.org/W2147921307","https://openalex.org/W2155495591","https://openalex.org/W2169105821","https://openalex.org/W2183980224","https://openalex.org/W2302115843","https://openalex.org/W2389413097","https://openalex.org/W2504172178","https://openalex.org/W2783981376"],"related_works":["https://openalex.org/W2141116096","https://openalex.org/W4386008020","https://openalex.org/W2756680690","https://openalex.org/W2483787835","https://openalex.org/W3091910256","https://openalex.org/W1979545076","https://openalex.org/W306968138","https://openalex.org/W2037555615","https://openalex.org/W1968568999","https://openalex.org/W2140402795"],"abstract_inverted_index":{"Strain":[0],"Enterococcus":[1,63],"sp.":[2,64],"GHB26,":[3],"isolated":[4],"from":[5,58,115],"the":[6,25,59,113,122],"Algerian":[7],"paste":[8],"of":[9,24,41,62,77,124,133,146,157,164],"dates":[10],"\"Ghars\",":[11],"produced":[12],"a":[13,81,89,101,109,130],"bacteriocin.":[14,126],"This":[15],"bacteriocin":[16,26,57,105,128,148],"was":[17,27,66,106],"inactivated":[18],"by":[19,68,74],"proteolytic":[20],"enzymes.":[21],"Antibacterial":[22],"activity":[23],"heat":[28],"stable":[29,37],"at":[30,38],"120\u00b0C":[31],"for":[32],"20":[33],"min":[34],"(533":[35],"AU/ml),":[36],"pH":[39],"range":[40],"2":[42],"to":[43,47,151],"12":[44],"and":[45,94],"resistant":[46],"chemicals":[48],"(SDS,":[49],"EDTA,":[50],"NaCl,":[51],"Tween":[52],"80,":[53],"Urea).":[54],"The":[55,104,127],"active":[56],"cell-free":[60],"supernatant":[61],"GHB26":[65],"purified":[67],"precipitation":[69],"with":[70],"ammonium":[71],"sulfate":[72],"followed":[73],"various":[75],"combinations":[76],"gel":[78,140],"filtration":[79],"on":[80,88,100,112],"Sephadex":[82,91],"G-25":[83],"column,":[84],"cation":[85],"exchange":[86],"chromatography":[87,99,120],"CM-":[90],"Cellulose":[92],"column":[93],"reverse":[95,116],"phase-high":[96,117],"performance":[97,118],"liquid":[98,119],"C18":[102],"column.":[103],"eluted":[107],"as":[108],"single":[110],"peak":[111],"chromatogram":[114],"attesting":[121],"purity":[123],"this":[125,147],"exhibited":[129],"bactericidal":[131],"mode":[132,163],"action.":[134,165],"Sodium":[135],"dodecyl":[136],"sulphate":[137],"-":[138],"polyacrylamide":[139],"electrophoresis":[141],"indicated":[142],"that":[143],"molecular":[144],"weight":[145],"is":[149],"close":[150],"3.5":[152],"kDa.":[153],"Key":[154],"words:":[155],"Paste":[156],"dates,":[158],"bacteriocin,":[159],"antibacterial":[160],"activity,":[161],"chromatography,":[162]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2016-07-22T00:00:00"}