{"id":"https://openalex.org/W2462242576","doi":"https://doi.org/10.3233/ch-1987-7405","title":"Evaluation of HEPES and phosphate buffers for rheological studies of sickle cells","display_name":"Evaluation of HEPES and phosphate buffers for rheological studies of sickle cells","publication_year":1987,"publication_date":"1987-08-01","ids":{"openalex":"https://openalex.org/W2462242576","doi":"https://doi.org/10.3233/ch-1987-7405","mag":"2462242576"},"language":"en","primary_location":{"id":"doi:10.3233/ch-1987-7405","doi":"https://doi.org/10.3233/ch-1987-7405","is_oa":false,"landing_page_url":"https://doi.org/10.3233/ch-1987-7405","pdf_url":null,"source":{"id":"https://openalex.org/S74293116","display_name":"Clinical Hemorheology and Microcirculation","issn_l":"1386-0291","issn":["1386-0291","1875-8622"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310318577","host_organization_name":"IOS Press","host_organization_lineage":["https://openalex.org/P4310318577"],"host_organization_lineage_names":["IOS Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Hemorheology and Microcirculation","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2462242576"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075962637","display_name":"A. J. Keidan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79619799","display_name":"University of Birmingham","ror":"https://ror.org/03angcq70","country_code":"GB","type":"education","lineage":["https://openalex.org/I79619799"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"A.J. Keidan","raw_affiliation_strings":["Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK","institution_ids":["https://openalex.org/I79619799"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108462700","display_name":"Martin C Sowter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79619799","display_name":"University of Birmingham","ror":"https://ror.org/03angcq70","country_code":"GB","type":"education","lineage":["https://openalex.org/I79619799"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"M.C. Sowter","raw_affiliation_strings":["Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK","institution_ids":["https://openalex.org/I79619799"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081603599","display_name":"Sukhjinder Marwah","orcid":"https://orcid.org/0000-0001-8972-4723","observed_orcids":["https://orcid.org/0000-0001-8972-4723"]},"institutions":[{"id":"https://openalex.org/I79619799","display_name":"University of Birmingham","ror":"https://ror.org/03angcq70","country_code":"GB","type":"education","lineage":["https://openalex.org/I79619799"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"S.S. Marwah","raw_affiliation_strings":["Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK","institution_ids":["https://openalex.org/I79619799"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064233250","display_name":"Cage Saul Johnson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79619799","display_name":"University of Birmingham","ror":"https://ror.org/03angcq70","country_code":"GB","type":"education","lineage":["https://openalex.org/I79619799"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"C.S. Johnson","raw_affiliation_strings":["Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK","institution_ids":["https://openalex.org/I79619799"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109913692","display_name":"J. Stuart","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79619799","display_name":"University of Birmingham","ror":"https://ror.org/03angcq70","country_code":"GB","type":"education","lineage":["https://openalex.org/I79619799"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"J. Stuart","raw_affiliation_strings":["Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Haematology, The Medical School, University of Birmingham, Birmingham B15 2TJ, UK","institution_ids":["https://openalex.org/I79619799"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5109913692"],"corresponding_institution_ids":["https://openalex.org/I79619799"],"apc_list":{"value":4150,"currency":"USD","value_usd":4150},"apc_paid":null,"fwci":2.4352,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.89511754,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"7","issue":"4","first_page":"599","last_page":"610"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10554","display_name":"Hemoglobinopathies and Related Disorders","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10554","display_name":"Hemoglobinopathies and Related Disorders","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11721","display_name":"Blood properties and coagulation","score":0.00860000029206276,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12286","display_name":"Erythrocyte Function and Pathophysiology","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sickle-cells","display_name":"sickle cells","score":0.8939999938011169},{"id":"https://openalex.org/keywords/hepes-buffer","display_name":"HEPES buffer","score":0.9459999799728394},{"id":"https://openalex.org/keywords/phosphate-buffer","display_name":"phosphate buffer","score":0.9980000257492065},{"id":"https://openalex.org/keywords/erythrocyte-rheology","display_name":"erythrocyte rheology","score":0.8769999742507935},{"id":"https://openalex.org/keywords/sickle-cell-disease","display_name":"sickle cell disease","score":0.7739999890327454},{"id":"https://openalex.org/keywords/erythrocyte-deformability","display_name":"erythrocyte deformability","score":0.9739999771118164},{"id":"https://openalex.org/keywords/cell-incubation","display_name":"cell incubation","score":0.43799999356269836},{"id":"https://openalex.org/keywords/oxygen-affinity","display_name":"oxygen affinity","score":0.7139999866485596},{"id":"https://openalex.org/keywords/hemolysis","display_name":"hemolysis","score":0.7179999947547913},{"id":"https://openalex.org/keywords/osmolality","display_name":"osmolality","score":0.5929999947547913},{"id":"https://openalex.org/keywords/atp-content","display_name":"ATP levels","score":0.4779999852180481}],"concepts":[{"id":"https://openalex.org/C2776816875","wikidata":"https://www.wikidata.org/wiki/Q418359","display_name":"HEPES","level":2,"score":0.7516499161720276},{"id":"https://openalex.org/C2778620579","wikidata":"https://www.wikidata.org/wiki/Q185034","display_name":"Sickle cell anemia","level":3,"score":0.6066120266914368},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.553275465965271},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.5240771174430847},{"id":"https://openalex.org/C2993207723","wikidata":"https://www.wikidata.org/wiki/Q4927803","display_name":"Oxygen tension","level":3,"score":0.5169857740402222},{"id":"https://openalex.org/C25642318","wikidata":"https://www.wikidata.org/wiki/Q1992532","display_name":"Incubation","level":2,"score":0.5102779865264893},{"id":"https://openalex.org/C2776557347","wikidata":"https://www.wikidata.org/wiki/Q37187","display_name":"Red blood cell","level":2,"score":0.477899432182312},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4486428201198578},{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.4437209367752075},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.42162439227104187},{"id":"https://openalex.org/C96616956","wikidata":"https://www.wikidata.org/wiki/Q285100","display_name":"Phosphate buffered saline","level":2,"score":0.41630133986473083},{"id":"https://openalex.org/C540031477","wikidata":"https://www.wikidata.org/wiki/Q629","display_name":"Oxygen","level":2,"score":0.39908894896507263},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3507014811038971},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.33862751722335815},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.270230770111084},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2295977771282196},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3233/ch-1987-7405","doi":"https://doi.org/10.3233/ch-1987-7405","is_oa":false,"landing_page_url":"https://doi.org/10.3233/ch-1987-7405","pdf_url":null,"source":{"id":"https://openalex.org/S74293116","display_name":"Clinical Hemorheology and Microcirculation","issn_l":"1386-0291","issn":["1386-0291","1875-8622"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310318577","host_organization_name":"IOS Press","host_organization_lineage":["https://openalex.org/P4310318577"],"host_organization_lineage_names":["IOS Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical Hemorheology and Microcirculation","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8560000061988831}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2513790780","https://openalex.org/W2002430405","https://openalex.org/W2563382558","https://openalex.org/W86450761","https://openalex.org/W2444043610","https://openalex.org/W2408136486","https://openalex.org/W2421202131","https://openalex.org/W136271931","https://openalex.org/W1995876797","https://openalex.org/W2102715003"],"abstract_inverted_index":{"Determination":[0],"of":[1,8,12,19,28,91],"the":[2,95],"optimal":[3],"buffer":[4],"for":[5,15,24,57,105],"rheological":[6],"studies":[7,27],"sickle":[9,34,41,92],"cells":[10,42,93],"is":[11],"particular":[13],"importance":[14],"in":[16,31,46,65],"vitro":[17],"screening":[18],"potential":[20],"anti-sickling":[21],"compounds":[22],"and":[23,86,108],"ex":[25],"vivo":[26],"erythrocyte":[29,109],"deformability":[30],"patients":[32],"with":[33,53],"cell":[35,70,73],"disease.":[36],"We":[37],"have":[38],"shown":[39],"that":[40],"can":[43],"be":[44],"incubated":[45],"HEPES":[47,52],"buffered":[48],"saline":[49],"(20":[50],"mmol/l":[51,55],"5":[54,82],"glucose)":[56],"6":[58,96],"hours":[59],"at":[60],"37\u00b0C":[61],"without":[62],"significant":[63],"change":[64],"morphology,":[66],"haemolytic":[67],"rate,":[68],"mean":[69,72],"volume,":[71],"haemoglobin":[74],"concentration,":[75],"osmolality,":[76],"pH,":[77],"ATP":[78],"content,":[79],"filterability":[80],"through":[81],"\u00b5m":[83],"diameter":[84],"pores,":[85],"oxygen":[87,106],"affinity.":[88],"Continuous":[89],"mixing":[90],"during":[94],"hour":[97],"incubation":[98],"was":[99],"essential":[100],"to":[101],"maintain":[102],"stable":[103],"values":[104],"tension":[107],"rheology.":[110]},"counts_by_year":[],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-07-22T00:00:00"}