{"id":"https://openalex.org/W2444099748","doi":"https://doi.org/10.1385/1-59259-830-7:263","title":"Keratinocyte Transient Transfections","display_name":"Keratinocyte Transient Transfections","publication_year":2004,"publication_date":"2004-10-21","ids":{"openalex":"https://openalex.org/W2444099748","doi":"https://doi.org/10.1385/1-59259-830-7:263","mag":"2444099748","pmid":"https://pubmed.ncbi.nlm.nih.gov/15502191"},"language":"en","primary_location":{"id":"doi:10.1385/1-59259-830-7:263","doi":"https://doi.org/10.1385/1-59259-830-7:263","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-830-7:263","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Epidermal Cells","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4255460153","display_name":"Epidermal Cells"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Anthony M. Flores","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I140172145","display_name":"University of Connecticut","ror":"https://ror.org/02der9h97","country_code":"US","type":"education","lineage":["https://openalex.org/I140172145"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anthony M. Flores","raw_affiliation_strings":["Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, USA","institution_ids":["https://openalex.org/I140172145"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069952158","display_name":"Brian J. Aneskievich","orcid":"https://orcid.org/0000-0001-7440-1948","observed_orcids":["https://orcid.org/0000-0001-7440-1948"]},"institutions":[{"id":"https://openalex.org/I140172145","display_name":"University of Connecticut","ror":"https://ror.org/02der9h97","country_code":"US","type":"education","lineage":["https://openalex.org/I140172145"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian J. Aneskievich","raw_affiliation_strings":["Departments of Nutritional and Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs","University of connecticut"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Nutritional and Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs","institution_ids":["https://openalex.org/I140172145"]},{"raw_affiliation_string":"University of connecticut","institution_ids":["https://openalex.org/I140172145"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I140172145"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.15194989,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"289","issue":null,"first_page":"263","last_page":"272"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.7333999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.7333999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0568000003695488,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.03400000184774399,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transient-transfection","display_name":"transient transfection","score":0.9879999756813049},{"id":"https://openalex.org/keywords/keratinocytes","display_name":"keratinocytes","score":0.9990000128746033},{"id":"https://openalex.org/keywords/cell-culture-optimization","display_name":"cell culture optimization","score":0.5730000138282776},{"id":"https://openalex.org/keywords/plasmid-isolation","display_name":"plasmid isolation","score":0.8529999852180481},{"id":"https://openalex.org/keywords/promoter-strength","display_name":"promoter strength","score":0.7429999709129333}],"concepts":[{"id":"https://openalex.org/C2780799671","wikidata":"https://www.wikidata.org/wiki/Q17087362","display_name":"Transient (computer programming)","level":2,"score":0.5993976593017578},{"id":"https://openalex.org/C2777074287","wikidata":"https://www.wikidata.org/wiki/Q1473931","display_name":"Keratinocyte","level":3,"score":0.5908414721488953},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5477433204650879},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.48855385184288025},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.48519280552864075},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.464655339717865},{"id":"https://openalex.org/C154020017","wikidata":"https://www.wikidata.org/wiki/Q520171","display_name":"Repeatability","level":2,"score":0.4134526252746582},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3862433433532715},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32201796770095825},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2902379035949707},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09345197677612305},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0864228904247284},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.08023294806480408},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.06649401783943176},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002130","descriptor_name":"Calcium Phosphates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D015603","descriptor_name":"Keratinocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1385/1-59259-830-7:263","doi":"https://doi.org/10.1385/1-59259-830-7:263","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-830-7:263","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Epidermal Cells","raw_type":"book-chapter"},{"id":"pmid:15502191","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15502191","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"},{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W20371925","https://openalex.org/W1980140028","https://openalex.org/W2014906721","https://openalex.org/W2071807226","https://openalex.org/W2091956097"],"related_works":["https://openalex.org/W2014906721","https://openalex.org/W2063441166","https://openalex.org/W2417169155","https://openalex.org/W1973015471","https://openalex.org/W2009399500","https://openalex.org/W1971117752","https://openalex.org/W2036418951","https://openalex.org/W2018719197","https://openalex.org/W1552066155","https://openalex.org/W1976491415"],"abstract_inverted_index":{"The":[0,28,87,109],"goal":[1],"of":[2,26],"this":[3],"chapter":[4],"is":[5,30,91,101,107],"to":[6,15,44,58,117,124],"give":[7],"the":[8,98,119,125,131],"reader":[9],"a":[10],"concise":[11],"and":[12,23,34,42,48,78,85,97,122,138,147],"easy-to-follow":[13],"guide":[14,118],"proven":[16],"transient":[17],"transfection":[18,61],"techniques":[19,56,70],"for":[20,40,67,74,93,103],"primary":[21],"strains":[22],"continuous":[24],"lines":[25,77],"keratinocytes.":[27],"emphasis":[29],"on":[31,135],"readily":[32],"available":[33],"inexpensive":[35],"resources":[36],"that":[37,128],"also":[38,71],"allow":[39],"repeatability":[41],"adaptability":[43],"scale,":[45],"experimental":[46,95],"conditions,":[47],"sample":[49],"replicates.":[50],"In":[51],"addition,":[52],"basic":[53],"cell":[54,144],"culture":[55,145],"necessary":[57,114],"achieve":[59],"optimal":[60],"results":[62],"are":[63],"provided.":[64],"Although":[65],"specified":[66],"keratinocytes,":[68],"these":[69],"work":[72],"well":[73],"simple":[75],"epithelial":[76],"common":[79],"laboratory":[80],"lines,":[81],"such":[82],"as":[83],"COS-7":[84],"CV-1.":[86],"standard":[88],"precipitation":[89],"protocol":[90,100,132],"suitable":[92],"many":[94],"designs,":[96],"poly-L-ornithine":[99],"intended":[102],"situations":[104],"where":[105],"calcium":[106],"contraindicated.":[108],"extended":[110],"notes":[111],"section":[112],"provides":[113],"technical":[115],"details":[116],"novice":[120],"user":[121],"reminders":[123],"experienced":[126],"scientist":[127],"success":[129],"with":[130],"will":[133],"depend":[134],"several":[136],"quantitative":[137],"qualitative":[139],"factors":[140],"including":[141],"promoter":[142],"strength,":[143],"practices":[146],"plasmid":[148],"isolation.":[149]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
