{"id":"https://openalex.org/W2437499447","doi":"https://doi.org/10.1007/978-1-62703-971-0_20","title":"Polyadenylation of RNA in E. coli: RNA Polymerase-Associated (rA)n-Synthetic Activities","display_name":"Polyadenylation of RNA in E. coli: RNA Polymerase-Associated (rA)n-Synthetic Activities","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W2437499447","doi":"https://doi.org/10.1007/978-1-62703-971-0_20","mag":"2437499447","pmid":"https://pubmed.ncbi.nlm.nih.gov/24590794"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-62703-971-0_20","doi":"https://doi.org/10.1007/978-1-62703-971-0_20","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-971-0_20","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4241369222","display_name":"Polyadenylation"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089191805","display_name":"Grant A. Encalade","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177898655","display_name":"Lamar University","ror":"https://ror.org/008ms5s18","country_code":"US","type":"education","lineage":["https://openalex.org/I177898655"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Grant A. Encalade","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Lamar University, 4400 MLK Boulevard, 10009, Beaumont, TX, 77710, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Lamar University, 4400 MLK Boulevard, 10009, Beaumont, TX, 77710, USA","institution_ids":["https://openalex.org/I177898655"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5057935319","display_name":"Maxim V. Sukhodolets","orcid":"https://orcid.org/0000-0001-8056-6570","observed_orcids":["https://orcid.org/0000-0001-8056-6570"]},"institutions":[{"id":"https://openalex.org/I177898655","display_name":"Lamar University","ror":"https://ror.org/008ms5s18","country_code":"US","type":"education","lineage":["https://openalex.org/I177898655"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Maxim V. Sukhodolets","raw_affiliation_strings":["Department of Chemistry and Biochemistry, Lamar University, 4400 MLK Boulevard, 10009, Beaumont, TX, 77710, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, Lamar University, 4400 MLK Boulevard, 10009, Beaumont, TX, 77710, USA","institution_ids":["https://openalex.org/I177898655"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5057935319"],"corresponding_institution_ids":["https://openalex.org/I177898655"],"apc_list":null,"apc_paid":null,"fwci":1.2653,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.81525502,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"1125","issue":null,"first_page":"251","last_page":"262"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.9526000022888184,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.9526000022888184,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.033799998462200165,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polyadenylation","display_name":"polyadenylation","score":0.9509999752044678},{"id":"https://openalex.org/keywords/poly-a-polymerase","display_name":"poly(A) polymerase","score":0.9200000166893005},{"id":"https://openalex.org/keywords/polynucleotide-phosphorylase","display_name":"polynucleotide phosphorylase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.796999990940094},{"id":"https://openalex.org/keywords/protein-fractionation","display_name":"protein fractionation","score":0.6880000233650208}],"concepts":[{"id":"https://openalex.org/C2779478826","wikidata":"https://www.wikidata.org/wiki/Q24780839","display_name":"Polynucleotide phosphorylase","level":5,"score":0.7936428189277649},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6436558961868286},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6348332166671753},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6201092600822449},{"id":"https://openalex.org/C71615608","wikidata":"https://www.wikidata.org/wiki/Q24720082","display_name":"Purine nucleoside phosphorylase","level":4,"score":0.5980316400527954},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.5894327163696289},{"id":"https://openalex.org/C142575336","wikidata":"https://www.wikidata.org/wiki/Q412148","display_name":"Polyadenylation","level":4,"score":0.562208890914917},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.5621142983436584},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.55571448802948},{"id":"https://openalex.org/C2776449523","wikidata":"https://www.wikidata.org/wiki/Q272631","display_name":"RNA polymerase","level":4,"score":0.5333376526832581},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.49776318669319153},{"id":"https://openalex.org/C2776543447","wikidata":"https://www.wikidata.org/wiki/Q28734","display_name":"Nucleoside","level":2,"score":0.4830625653266907},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.46389225125312805},{"id":"https://openalex.org/C111921641","wikidata":"https://www.wikidata.org/wiki/Q80756","display_name":"Polynucleotide","level":2,"score":0.46353888511657715},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4502538740634918},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37129247188568115},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09934952855110168},{"id":"https://openalex.org/C2776476023","wikidata":"https://www.wikidata.org/wiki/Q188261","display_name":"Purine","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012329","descriptor_name":"RNA, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012329","descriptor_name":"RNA, Bacterial","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D026723","descriptor_name":"Polyadenylation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-62703-971-0_20","doi":"https://doi.org/10.1007/978-1-62703-971-0_20","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-971-0_20","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:24590794","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24590794","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4588164164","display_name":null,"funder_award_id":"GM81803","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G488172638","display_name":"Substrate Specificity and Mechanism of the Bacterial SWI/SNF Protein RapA","funder_award_id":"R15GM081803","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1504479686","https://openalex.org/W1505365127","https://openalex.org/W1523841046","https://openalex.org/W1529084957","https://openalex.org/W1653560622","https://openalex.org/W1979837084","https://openalex.org/W1983858947","https://openalex.org/W2023002165","https://openalex.org/W2034857418","https://openalex.org/W2066806444","https://openalex.org/W2080016008","https://openalex.org/W2095062054","https://openalex.org/W2112491244","https://openalex.org/W2144981232"],"related_works":["https://openalex.org/W2095062054","https://openalex.org/W1493182500","https://openalex.org/W2144981232","https://openalex.org/W6995796544","https://openalex.org/W1992792121","https://openalex.org/W1980163639","https://openalex.org/W2104873932","https://openalex.org/W2016275655","https://openalex.org/W1556919838","https://openalex.org/W2069170254"],"abstract_inverted_index":{"In":[0,25],"Escherichia":[1],"coli,":[2],"Poly(A)":[3],"polymerase":[4],"(PAP)":[5],"and":[6,55,74,103],"polynucleotide":[7],"phosphorylase":[8],"(PNP)":[9],"are":[10],"key":[11],"enzymes":[12],"thought":[13],"to":[14,65],"be":[15,58],"responsible":[16],"for":[17,34,85],"polyadenylation":[18],"of":[19,22,53,68,89,96,110],"the":[20,66,77,87],"bulk":[21],"cellular":[23],"RNA.":[24],"this":[26],"chapter":[27],"we":[28],"describe":[29,81],"enzymatic":[30,51],"in":[31],"vitro":[32],"assays":[33],"monitoring":[35,86],"(rA)n-synthetic":[36],"activity":[37],"among":[38,61],"fractionated":[39,62],"E.":[40],"coli":[41],"proteins":[42,63],"obtained":[43],"after":[44],"affinity":[45],"chromatography":[46],"on":[47,94,108],"immobilized":[48],"DNA.":[49],"The":[50],"activities":[52],"PAP":[54],"PNP":[56],"can":[57],"independently":[59],"monitored":[60],"due":[64],"utilization":[67,95,109],"different":[69,83],"nucleoside":[70,112],"substrates":[71],"(respectively,":[72],"ATP":[73],"ADP)":[75],"by":[76],"two":[78,82],"enzymes.":[79],"We":[80],"methods":[84],"synthesis":[88],"polyadenylate:":[90],"a":[91,97],"method":[92,106],"based":[93,107],"nucleic":[98],"acid-specific":[99],"fluorescent":[100],"dye":[101],"(RiboGreen(\u00ae))":[102],"an":[104],"alternative":[105],"P(32)-labeled":[111],"phosphates.":[113]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
