{"id":"https://openalex.org/W2431713845","doi":"https://doi.org/10.1152/ajpcell.1998.275.3.c732","title":"Optical measurement of stimulus-evoked membrane dynamics in single pancreatic acinar cells","display_name":"Optical measurement of stimulus-evoked membrane dynamics in single pancreatic acinar cells","publication_year":1998,"publication_date":"1998-09-01","ids":{"openalex":"https://openalex.org/W2431713845","doi":"https://doi.org/10.1152/ajpcell.1998.275.3.c732","mag":"2431713845","pmid":"https://pubmed.ncbi.nlm.nih.gov/9730956"},"language":"en","primary_location":{"id":"doi:10.1152/ajpcell.1998.275.3.c732","doi":"https://doi.org/10.1152/ajpcell.1998.275.3.c732","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpcell.1998.275.3.c732","pdf_url":null,"source":{"id":"https://openalex.org/S62704344","display_name":"American Journal of Physiology-Cell Physiology","issn_l":"0363-6143","issn":["0363-6143","1522-1563"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Cell Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082353619","display_name":"David R. Giovannucci","orcid":"https://orcid.org/0000-0002-8813-047X","observed_orcids":["https://orcid.org/0000-0002-8813-047X"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David R. Giovannucci","raw_affiliation_strings":["Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","1 Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]},{"raw_affiliation_string":"1 Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006672847","display_name":"David I. Yule","orcid":"https://orcid.org/0000-0002-6743-0668","observed_orcids":["https://orcid.org/0000-0002-6743-0668"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David I. Yule","raw_affiliation_strings":["Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","1 Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]},{"raw_affiliation_string":"1 Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075391488","display_name":"Edward L. Stuenkel","orcid":"https://orcid.org/0000-0001-8225-5725","observed_orcids":["https://orcid.org/0000-0001-8225-5725"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edward L. Stuenkel","raw_affiliation_strings":["Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","1 Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]},{"raw_affiliation_string":"1 Department of Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.8399,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.72853369,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"275","issue":"3","first_page":"C732","last_page":"C739"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10839","display_name":"Pancreatic function and diabetes","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pancreatic-acinar-cells","display_name":"pancreatic acinar cells","score":1.0},{"id":"https://openalex.org/keywords/membrane-capacitance","display_name":"membrane capacitance","score":0.6970000267028809},{"id":"https://openalex.org/keywords/fm1-43","display_name":"FM1-43","score":0.8330000042915344},{"id":"https://openalex.org/keywords/membrane-area","display_name":"membrane surface area","score":0.7139999866485596},{"id":"https://openalex.org/keywords/endocytosis","display_name":"endocytosis","score":0.7850000262260437},{"id":"https://openalex.org/keywords/endosomes","display_name":"endosomes","score":0.6520000100135803},{"id":"https://openalex.org/keywords/receptor-recycling","display_name":"receptor recycling","score":0.4790000021457672},{"id":"https://openalex.org/keywords/digestive-enzyme-secretion","display_name":"digestive enzyme secretion","score":0.6949999928474426},{"id":"https://openalex.org/keywords/hormone-stimulation","display_name":"hormonal stimulation","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.7119840383529663},{"id":"https://openalex.org/C2991930265","wikidata":"https://www.wikidata.org/wiki/Q5062943","display_name":"Acinar cell","level":3,"score":0.6726081371307373},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.5696777701377869},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.5625090599060059},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5404163002967834},{"id":"https://openalex.org/C136009344","wikidata":"https://www.wikidata.org/wiki/Q336201","display_name":"Confocal","level":2,"score":0.5166471600532532},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.45842257142066956},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4481835663318634},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19847452640533447},{"id":"https://openalex.org/C2778764654","wikidata":"https://www.wikidata.org/wiki/Q9618","display_name":"Pancreas","level":2,"score":0.1804807186126709},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16652193665504456},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000644","descriptor_name":"Quaternary Ammonium Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002217","descriptor_name":"Carbachol","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D003594","descriptor_name":"Cytoplasmic Granules","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005089","descriptor_name":"Exocytosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008561","descriptor_name":"Membrane Fusion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008564","descriptor_name":"Membrane Potentials","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D008564","descriptor_name":"Membrane Potentials","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D010179","descriptor_name":"Pancreas","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D010179","descriptor_name":"Pancreas","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D010179","descriptor_name":"Pancreas","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D011726","descriptor_name":"Pyridinium Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016244","descriptor_name":"Guanosine 5'-O-(3-Thiotriphosphate)","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1152/ajpcell.1998.275.3.c732","doi":"https://doi.org/10.1152/ajpcell.1998.275.3.c732","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpcell.1998.275.3.c732","pdf_url":null,"source":{"id":"https://openalex.org/S62704344","display_name":"American Journal of Physiology-Cell Physiology","issn_l":"0363-6143","issn":["0363-6143","1522-1563"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Cell Physiology","raw_type":"journal-article"},{"id":"pmid:9730956","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9730956","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The American journal of physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1579498119","display_name":null,"funder_award_id":"DK-41122","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G6837720967","display_name":null,"funder_award_id":"NS36227","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G8616237017","display_name":"SNARE HYPOTHESIS AT NERVE ENDINGS","funder_award_id":"5r01ns036227-05","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W249983744","https://openalex.org/W303549700","https://openalex.org/W1520251373","https://openalex.org/W1528038969","https://openalex.org/W1585919450","https://openalex.org/W1922988348","https://openalex.org/W1964395778","https://openalex.org/W1969422421","https://openalex.org/W1976680575","https://openalex.org/W1977440143","https://openalex.org/W1978627586","https://openalex.org/W1981344194","https://openalex.org/W1981761927","https://openalex.org/W1982760200","https://openalex.org/W1986542931","https://openalex.org/W1991972640","https://openalex.org/W1994140164","https://openalex.org/W1994622457","https://openalex.org/W1995408976","https://openalex.org/W2007461061","https://openalex.org/W2007669144","https://openalex.org/W2015610759","https://openalex.org/W2020508938","https://openalex.org/W2024427902","https://openalex.org/W2025262639","https://openalex.org/W2031521834","https://openalex.org/W2034791541","https://openalex.org/W2038328161","https://openalex.org/W2043623389","https://openalex.org/W2046645924","https://openalex.org/W2048996308","https://openalex.org/W2051842061","https://openalex.org/W2055450655","https://openalex.org/W2059401109","https://openalex.org/W2068665047","https://openalex.org/W2068950919","https://openalex.org/W2073125666","https://openalex.org/W2122247317","https://openalex.org/W2136839561","https://openalex.org/W2141022591","https://openalex.org/W2141255220","https://openalex.org/W2154028120","https://openalex.org/W2170873164","https://openalex.org/W2176210399","https://openalex.org/W2260202993","https://openalex.org/W2296738195","https://openalex.org/W2412907966"],"related_works":["https://openalex.org/W2138549120","https://openalex.org/W2074776189","https://openalex.org/W2051617726","https://openalex.org/W2025262639","https://openalex.org/W2460614951","https://openalex.org/W2111458472","https://openalex.org/W2013816456","https://openalex.org/W2419207932","https://openalex.org/W2145490180","https://openalex.org/W93594259"],"abstract_inverted_index":{"Stimulation":[0],"of":[1,8,15,36,94,101,106,133,138,147,155,160],"pancreatic":[2,39],"acinar":[3,40,71,134,149,166],"cells":[4,41,167],"induces":[5],"the":[6,12,28,52,60,92,109,130,144],"release":[7],"digestive":[9],"enzymes":[10],"via":[11],"exocytotic":[13,161],"fusion":[14,93],"zymogen":[16,96],"granules":[17,97],"and":[18,24,50,127,162],"activates":[19],"postfusion":[20],"granule":[21],"membrane":[22,33,46,53,145],"retrieval":[23],"receptor":[25],"cycling.":[26],"In":[27,78],"present":[29],"study,":[30],"changes":[31],"in":[32,70,83,124,151,165],"surface":[34,73,84],"area":[35,74,85],"rat":[37],"single":[38,148],"were":[42],"monitored":[43],"by":[44,51,118],"cell":[45,72],"capacitance":[47],"(Cm)":[48],"measurements":[49,154],"fluorescent":[54],"dye":[55],"FM1-43.":[56],"When":[57],"measured":[58],"with":[59,153],"Cm":[61,156],"method,":[62],"agonist":[63],"treatment":[64],"evoked":[65],"a":[66,80,99,112],"graded,":[67],"transient":[68],"increase":[69,82],"averaging":[75],"3.":[76],"5%.":[77],"contrast,":[79],"13%":[81],"was":[86,116],"estimated":[87],"using":[88],"FM1-43,":[89],"corresponding":[90],"to":[91,121,129],"48":[95],"at":[98],"rate":[100],"0.5":[102],"s-1.":[103],"After":[104],"removal":[105],"FM1-43":[107],"from":[108],"surface-accessible":[110],"membrane,":[111],"residual":[113],"fluorescence":[114],"signal":[115],"shown":[117],"confocal":[119],"microscopy":[120],"be":[122],"localized":[123],"endosome-like":[125],"structures":[126],"confined":[128],"apical":[131],"regions":[132],"cells.":[135],"The":[136],"development":[137],"an":[139],"optical":[140],"method":[141],"for":[142],"monitoring":[143],"turnover":[146],"cells,":[150],"combination":[152],"changes,":[157],"reveals":[158],"coincidence":[159],"endocytotic":[163],"activity":[164],"after":[168],"hormonal":[169],"stimulation.":[170]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}