{"id":"https://openalex.org/W2422957134","doi":null,"title":"[Heterogenous expression and characterization of cag4 in Helicobacter pylori].","display_name":"[Heterogenous expression and characterization of cag4 in Helicobacter pylori].","publication_year":2011,"publication_date":"2011-05-01","ids":{"openalex":"https://openalex.org/W2422957134","mag":"2422957134","pmid":"https://pubmed.ncbi.nlm.nih.gov/21800625"},"language":"zh","primary_location":{"id":"pmid:21800625","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21800625","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Wei sheng wu xue bao = Acta microbiologica Sinica","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100720748","display_name":"Wenkai Wang","orcid":"https://orcid.org/0000-0002-9524-1581","observed_orcids":["https://orcid.org/0000-0002-9524-1581"]},"institutions":[{"id":"https://openalex.org/I115592961","display_name":"Jiangsu University","ror":"https://ror.org/03jc41j30","country_code":"CN","type":"education","lineage":["https://openalex.org/I115592961"]},{"id":"https://openalex.org/I4210111628","display_name":"Affiliated Hospital of Jiangsu University","ror":"https://ror.org/028pgd321","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210111628"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenkai Wang","raw_affiliation_strings":["Clinical Laboratory, School of medical technology, the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China. wangwenkai3000@yahoo.com.cn"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Laboratory, School of medical technology, the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China. wangwenkai3000@yahoo.com.cn","institution_ids":["https://openalex.org/I115592961","https://openalex.org/I4210111628"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Qiao Zhong","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Qiao Zhong","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026948419","display_name":"Liping Xie","orcid":"https://orcid.org/0000-0002-6900-1883","observed_orcids":["https://orcid.org/0000-0002-6900-1883"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Liping Xie","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5007058151","display_name":"Shihe Shao","orcid":"https://orcid.org/0000-0001-9883-772X","observed_orcids":["https://orcid.org/0000-0001-9883-772X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shihe Shao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"51","issue":"5","first_page":"630","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10276","display_name":"Helicobacter pylori-related gastroenterology studies","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2715","display_name":"Gastroenterology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10276","display_name":"Helicobacter pylori-related gastroenterology studies","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2715","display_name":"Gastroenterology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/helicobacter-pylori","display_name":"Helicobacter pylori","score":0.9739999771118164},{"id":"https://openalex.org/keywords/cag-pathogenicity-island","display_name":"cag pathogenicity island","score":0.8489999771118164},{"id":"https://openalex.org/keywords/lytic-transglycosylase","display_name":"lytic transglycosylase","score":0.9359999895095825},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9610000252723694},{"id":"https://openalex.org/keywords/peptidoglycan-degradation","display_name":"peptidoglycan degradation","score":0.8330000042915344},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.7459999918937683},{"id":"https://openalex.org/keywords/ph-effect-on-enzyme-activity","display_name":"pH-dependent enzyme activity","score":0.7509999871253967},{"id":"https://openalex.org/keywords/protein-renaturation","display_name":"protein renaturation","score":0.4880000054836273}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.8455212712287903},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6710035800933838},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5767058730125427},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.573330819606781},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5319042205810547},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.530222475528717},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.5268453359603882},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5070829391479492},{"id":"https://openalex.org/C2776634448","wikidata":"https://www.wikidata.org/wiki/Q206920","display_name":"Peptidoglycan","level":3,"score":0.5066841244697571},{"id":"https://openalex.org/C182615771","wikidata":"https://www.wikidata.org/wiki/Q1369386","display_name":"Lytic cycle","level":3,"score":0.48926493525505066},{"id":"https://openalex.org/C2776409635","wikidata":"https://www.wikidata.org/wiki/Q180556","display_name":"Helicobacter pylori","level":2,"score":0.4875580668449402},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.4757941961288452},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.45522794127464294},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3312200903892517},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3169274926185608},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.10339832305908203},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016480","descriptor_name":"Helicobacter pylori","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016480","descriptor_name":"Helicobacter pylori","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016695","descriptor_name":"Glycosyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016695","descriptor_name":"Glycosyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":1,"locations":[{"id":"pmid:21800625","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21800625","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Wei sheng wu xue bao = Acta microbiologica Sinica","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2359613730","https://openalex.org/W2359260947","https://openalex.org/W2352794740","https://openalex.org/W2371153225","https://openalex.org/W2391255691","https://openalex.org/W2364616418","https://openalex.org/W2353241298","https://openalex.org/W2380333359","https://openalex.org/W2943907233","https://openalex.org/W2108295612"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"construct":[2],"prokaryotic":[3,55],"fusion":[4,16],"gene":[5],"expression":[6,56,70],"vector":[7],"pET-28a-cag":[8],"pathogenicity":[9],"island":[10],"protein":[11,17,87,100,121,137],"4":[12],"(cag4),express":[13],"the":[14,21,61,85,98,116,126,139,151,180],"recombinant":[15,25,86,99,120,136],"cag4":[18,30,174],"and":[19,48,59,78,83,125,170],"analyze":[20],"enzyme":[22,50,117,140,146,181],"activity":[23,118,141,182],"of":[24,38,119,128,142,153,175,183],"protein.":[26],"METHODS:":[27],"We":[28,75,81,96,114],"cloned":[29],"gene,":[31],"this":[32],"encoded":[33],"lytic":[34,143,184],"transglycosylase":[35],"in":[36],"CagPAI":[37],"Helicobacter":[39,176],"pylori":[40,177],"NCTC11637":[41,178],"via":[42,101],"PCR":[43],"method.":[44,113],"After":[45],"TA":[46],"cloning":[47],"restriction":[49],"digested":[51],"confirmed,":[52],"we":[53,104],"constructed":[54],"plasmid":[57,62],"pET-28a-cag4":[58],"transformed":[60],"into":[63],"E.":[64],"coli":[65],"BL21":[66],"(DE3)":[67],"for":[68],"heterogenesis":[69],"after":[71],"it":[72],"is":[73],"sequenced.":[74],"performed":[76],"SDS-PAGE":[77],"Western":[79],"blot.":[80],"purified":[82],"collected":[84],"by":[88,110,122,131],"using":[89],"Ni2+":[90],"-NTA":[91],"columns":[92],"under":[93],"denaturation":[94],"condition.":[95],"renatured":[97],"dialysis.":[102],"Besides,":[103],"isolated":[105],"peptidoglycan":[106],"from":[107],"Micrococcus":[108],"lysodeikticus":[109],"SDS":[111],"boiling":[112],"detected":[115],"zymogram":[123],"analysis":[124],"effect":[127],"pH":[129,162],"values":[130],"turbidimetric":[132],"analysis.":[133],"RESULTS:":[134],"The":[135,145,173],"has":[138,179],"transglycosylase.":[144,185],"activity,":[147],"which":[148],"defined":[149],"as":[150],"rate":[152],"degraded":[154],"((A":[155],"X":[156],"min(-1)":[157],"mg(-1)),":[158],"was":[159],"higher":[160],"at":[161],"6.0":[163],"group":[164],"than":[165],"other":[166],"groups":[167],"(pH":[168],"5.0":[169],"7.0).":[171],"CONCLUSION:":[172]},"counts_by_year":[{"year":2022,"cited_by_count":1}],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2016-06-24T00:00:00"}
