{"id":"https://openalex.org/W2422320201","doi":null,"title":"[Growth inhibitory effects of recombinant granzyme B containing different N-terminal translocating peptides].","display_name":"[Growth inhibitory effects of recombinant granzyme B containing different N-terminal translocating peptides].","publication_year":2004,"publication_date":"2004-07-01","ids":{"openalex":"https://openalex.org/W2422320201","mag":"2422320201","pmid":"https://pubmed.ncbi.nlm.nih.gov/15968978"},"language":"en","primary_location":{"id":"pmid:15968978","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15968978","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102023989","display_name":"Jing Zhao","orcid":"https://orcid.org/0000-0003-0764-8179","observed_orcids":["https://orcid.org/0000-0003-0764-8179"]},"institutions":[{"id":"https://openalex.org/I9916479","display_name":"Air Force Medical University","ror":"https://ror.org/00ms48f15","country_code":"CN","type":"education","lineage":["https://openalex.org/I9916479"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jing Zhao","raw_affiliation_strings":["Fourth Military Medical University, Department of Biochemistry and Molecular Biology, Xi' an 710032, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fourth Military Medical University, Department of Biochemistry and Molecular Biology, Xi' an 710032, China","institution_ids":["https://openalex.org/I9916479"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100376398","display_name":"Zhi Wang","orcid":"https://orcid.org/0000-0002-5276-1413","observed_orcids":["https://orcid.org/0000-0002-5276-1413"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhi Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100946008","display_name":"Cui-Juan Yu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Cui-Juan Yu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113747365","display_name":"Yunxin Cao","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yun-Xin Cao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100425554","display_name":"Li Zhang","orcid":"https://orcid.org/0000-0002-3617-2627","observed_orcids":["https://orcid.org/0000-0002-3617-2627","https://orcid.org/0000-0003-2756-2620","https://orcid.org/0009-0001-8129-4308"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Li Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004686722","display_name":"Chengji Wang","orcid":"https://orcid.org/0000-0003-3331-9117","observed_orcids":["https://orcid.org/0000-0003-3331-9117"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Cheng-Ji Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5100741624","display_name":"Angang Yang","orcid":"https://orcid.org/0000-0002-8854-4672","observed_orcids":["https://orcid.org/0000-0002-8854-4672"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"An-Gang Yang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.29852682,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"20","issue":"4","first_page":"501","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12996","display_name":"Cancer Research and Treatments","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/granzyme-b","display_name":"granzyme B","score":0.9990000128746033},{"id":"https://openalex.org/keywords/pseudomonas-exotoxin-a","display_name":"Pseudomonas exotoxin A","score":0.9150000214576721},{"id":"https://openalex.org/keywords/fusion-protein","display_name":"fusion protein","score":0.699999988079071},{"id":"https://openalex.org/keywords/serine-protease-activity","display_name":"serine protease activity","score":0.8489999771118164},{"id":"https://openalex.org/keywords/cell-growth-inhibition","display_name":"cell growth inhibition","score":0.781000018119812},{"id":"https://openalex.org/keywords/g2-cell-cycle-arrest","display_name":"G2 cell cycle arrest","score":0.9710000157356262},{"id":"https://openalex.org/keywords/cytotoxic-proteins","display_name":"cytotoxic proteins","score":0.6050000190734863},{"id":"https://openalex.org/keywords/tumor-cell-killing","display_name":"tumor cell killing","score":0.8159999847412109},{"id":"https://openalex.org/keywords/hela-cells","display_name":"HeLa cells","score":0.6150000095367432}],"concepts":[{"id":"https://openalex.org/C183193105","wikidata":"https://www.wikidata.org/wiki/Q425068","display_name":"Granzyme","level":5,"score":0.7869939208030701},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7132322788238525},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.6839584112167358},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6164840459823608},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.4228828251361847},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.4179005026817322},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.3939536511898041},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3097299635410309},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.2915133833885193},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.156014084815979},{"id":"https://openalex.org/C2776062744","wikidata":"https://www.wikidata.org/wiki/Q21103279","display_name":"Perforin","level":4,"score":0.12679129838943481},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.10041338205337524}],"mesh":[{"descriptor_ui":"D000097668","descriptor_name":"Pseudomonas aeruginosa Exotoxin A","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001427","descriptor_name":"Bacterial Toxins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001427","descriptor_name":"Bacterial Toxins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005098","descriptor_name":"Exotoxins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005098","descriptor_name":"Exotoxins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D036002","descriptor_name":"ADP Ribose Transferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D036002","descriptor_name":"ADP Ribose Transferases","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D037521","descriptor_name":"Virulence Factors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D037521","descriptor_name":"Virulence Factors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D053804","descriptor_name":"Granzymes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D053804","descriptor_name":"Granzymes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:15968978","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15968978","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","raw_type":"Journal Article"},{"id":"mag:2422320201","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15968978","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1028360203","https://openalex.org/W2375084441","https://openalex.org/W2388473351","https://openalex.org/W2047161536","https://openalex.org/W2064881016","https://openalex.org/W1000563790","https://openalex.org/W2386006581","https://openalex.org/W1422293486","https://openalex.org/W2086544446","https://openalex.org/W2124559492"],"abstract_inverted_index":{"Translocating":[0],"protein":[1,46,260],"and":[2,54,79,109,116,137,147,161,180,186,205,239,264],"translocating":[3,36,66,262],"peptides":[4,37,263],"have":[5,200,232],"therapeutic":[6],"potential":[7],"against":[8],"tumors":[9],"by":[10,50,76,105,220],"exposing":[11],"the":[12,19,30,130,188,241,256],"cytotoxic":[13,265],"domains":[14],"of":[15,24,32,58,68,132,169,176,183,190,258],"toxic":[16],"proteins":[17,173,231],"to":[18,28,199,237,254],"cell":[20,162,221,233,269],"cytosol.":[21],"The":[22,87,111,192],"aim":[23],"this":[25],"study":[26,252],"is":[27],"investigate":[29],"effect":[31,235],"N-terminally":[33],"fused":[34],"PE":[35,43,133,170,193,228],"on":[38,107,248],"granzyme":[39,60,184],"B":[40,61],"(GrBa)":[41],"activity.":[42,250],"II-GrBa":[44,90,134,171,229],"fusion":[45,135,172,230],"genes":[47],"were":[48,93,114,174],"constructed":[49],"replacing":[51],"N-terminal":[52],"signal":[53],"acidic":[55],"dipeptide":[56],"sequence":[57],"human":[59],"gene":[62],"with":[63,95,118,215],"two":[64,167],"truncated":[65],"sequences":[67],"Pseudomonas":[69],"exotoxin":[70],"A":[71,127],"(PE":[72],"II":[73,194],"aa":[74],"280-364/358)":[75],"recombinant":[77,259],"PCR,":[78],"then":[80],"cloned":[81],"into":[82,99],"pIND":[83],"inducible":[84],"expression":[85,91,131],"vector.":[86],"resulting":[88],"pIND-PE":[89],"vectors":[92],"co-transfected":[94],"assistant":[96],"plasmid":[97],"pVgRXR":[98],"HeLa":[100],"cells":[101,113],"through":[102],"lipofectamine,":[103],"followed":[104],"selection":[106],"G418":[108],"zeocin.":[110],"resistant":[112],"collected":[115],"induced":[117],"ponasterone":[119,126],"A.":[120],"Western":[121,156],"blot":[122],"analysis":[123,164],"demonstrated":[124],"that":[125,166,227,240],"induction":[128],"caused":[129],"proteins,":[136],"indirect":[138],"immunofluorescence":[139],"detected":[140],"giant":[141],"sized":[142],"multinucleated":[143],"cells,":[144],"suggesting":[145],"cytoskeletal":[146],"mitotic":[148],"abnormalities":[149],"as":[150,218],"reported":[151],"in":[152],"our":[153],"previous":[154],"studies.":[155],"blot,":[157],"enzymatic":[158],"activity":[159,204],"assay":[160],"counting":[163],"indicated":[165],"types":[168],"capable":[175],"cleaving":[177],"both":[178],"endogenous":[179],"exogenous":[181],"substrates":[182],"B,":[185],"inhibiting":[187],"growth":[189,207],"cells.":[191],"(aa":[195],"280-358)-GrBa":[196],"was":[197,212],"shown":[198],"higher":[201],"serine":[202],"protease":[203],"stronger":[206],"inhibitory":[208,234],"effect.":[209],"Such":[210],"inhibition":[211],"presumably":[213],"associated":[214],"G2":[216],"arrest":[217],"determined":[219],"cycle":[222],"analysis.":[223],"These":[224],"data":[225],"prove":[226],"similar":[236],"GrBa,":[238],"shorter":[242],"PE-derived":[243],"peptide":[244],"exerts":[245],"less":[246],"influence":[247],"GrBa":[249],"This":[251],"helps":[253],"optimize":[255],"construction":[257],"comprising":[261],"molecules":[266],"for":[267],"tumor":[268],"killing.":[270]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}