{"id":"https://openalex.org/W2420109025","doi":null,"title":"Epitope masking of rhabdomeric rhodopsin during endocytosis-induced retinal degeneration.","display_name":"Epitope masking of rhabdomeric rhodopsin during endocytosis-induced retinal degeneration.","publication_year":2002,"publication_date":"2002-12-02","ids":{"openalex":"https://openalex.org/W2420109025","mag":"2420109025","pmid":"https://pubmed.ncbi.nlm.nih.gov/12486400"},"language":"en","primary_location":{"id":"pmid:12486400","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12486400","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular vision","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040353601","display_name":"Nicholas R. Orem","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas R Orem","raw_affiliation_strings":["Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5064376675","display_name":"Patrick J. Dolph","orcid":"https://orcid.org/0000-0001-9770-727X","observed_orcids":["https://orcid.org/0000-0001-9770-727X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Patrick J Dolph","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.0065,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.79900972,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"8","issue":null,"first_page":"455","last_page":"61"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.9355000257492065,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.9355000257492065,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.04699999839067459,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.005200000014156103,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/arrestin","display_name":"arrestin","score":0.796999990940094},{"id":"https://openalex.org/keywords/epitope-masking","display_name":"epitope masking","score":0.9190000295639038},{"id":"https://openalex.org/keywords/endocytosis","display_name":"endocytosis","score":0.8560000061988831},{"id":"https://openalex.org/keywords/retinal-degeneration","display_name":"retinal degeneration","score":0.9850000143051147},{"id":"https://openalex.org/keywords/photoreceptors","display_name":"photoreceptors","score":0.8370000123977661},{"id":"https://openalex.org/keywords/rhabdomere","display_name":"rhabdomere","score":0.8489999771118164},{"id":"https://openalex.org/keywords/drosophila","display_name":"Drosophila","score":0.7900000214576721},{"id":"https://openalex.org/keywords/photoreceptor-apoptosis","display_name":"photoreceptor apoptosis","score":0.7919999957084656}],"concepts":[{"id":"https://openalex.org/C202033177","wikidata":"https://www.wikidata.org/wiki/Q423107","display_name":"Rhodopsin","level":3,"score":0.9741331338882446},{"id":"https://openalex.org/C2777503648","wikidata":"https://www.wikidata.org/wiki/Q905671","display_name":"Arrestin","level":4,"score":0.8616656064987183},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6501762270927429},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6032865047454834},{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.5754915475845337},{"id":"https://openalex.org/C2781040256","wikidata":"https://www.wikidata.org/wiki/Q7316761","display_name":"Retinal degeneration","level":3,"score":0.5657277703285217},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.5040103793144226},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.36013954877853394},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32444003224372864},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.24505147337913513},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23771312832832336},{"id":"https://openalex.org/C135285700","wikidata":"https://www.wikidata.org/wiki/Q38173","display_name":"G protein-coupled receptor","level":3,"score":0.10719585418701172},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.09921056032180786}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004331","descriptor_name":"Drosophila melanogaster","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004705","descriptor_name":"Endocytosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010738","descriptor_name":"Type C Phospholipases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010750","descriptor_name":"Phosphoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012162","descriptor_name":"Retinal Degeneration","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012243","descriptor_name":"Rhodopsin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D017956","descriptor_name":"Photoreceptor Cells, Invertebrate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019084","descriptor_name":"Fluorescent Antibody Technique, Indirect","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019390","descriptor_name":"Arrestins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D043265","descriptor_name":"Phosphatidylinositol Diacylglycerol-Lyase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:12486400","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12486400","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular vision","raw_type":"Journal Article"},{"id":"mag:2420109025","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12486400","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.4043000042438507}],"study_designs":[],"awards":[{"id":"https://openalex.org/G987989974","display_name":"TRANSDUCTION AND RETINAL DEGENERATION IN DROSOPHILA","funder_award_id":"R01EY011534","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2041281312","https://openalex.org/W1997853762","https://openalex.org/W1980289311","https://openalex.org/W2029507401","https://openalex.org/W2038392988","https://openalex.org/W2964324319","https://openalex.org/W4240294458","https://openalex.org/W6964290090","https://openalex.org/W1967281511","https://openalex.org/W2061042616"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1],"determine":[2],"the":[3,66,84,100,107,115,133,142,166,169,173,185,191,204,209,227,234],"fate":[4],"of":[5,31,52,68,77,102,117,122,160,168,175,184,203,236],"rhodopsin":[6,48,79,105,118,125,129,151,163,186,205,219],"during":[7,83,198],"endocytosis-mediated":[8,70],"retinal":[9,71,200,244],"degeneration.":[10],"METHODS:":[11],"Drosophila":[12],"stocks":[13],"were":[14,37],"raised":[15],"in":[16,65,114,165,212,226,233,243],"complete":[17],"darkness":[18],"and":[19,29,93,112,152],"shifted":[20],"to":[21,27,104,162,208],"light":[22,94],"for":[23,47,239],"24":[24],"h":[25],"prior":[26],"dissection":[28],"fixation":[30],"retinas.":[32],"1":[33],"microm":[34],"frozen":[35],"sections":[36],"cut":[38],"on":[39,172],"an":[40,74],"ultracryomicrotome,":[41],"then":[42],"stained":[43],"with":[44,147],"antibodies":[45],"specific":[46],"or":[49],"arrestin.":[50,148],"Localization":[51],"photoreceptor":[53,240],"cell-specific":[54],"proteins":[55],"was":[56,130,139],"determined":[57],"by":[58],"confocal":[59],"microscopy.":[60],"RESULTS:":[61],"Flies":[62],"that":[63,99,137,197,218],"are":[64,154,231],"process":[67],"undergoing":[69],"degeneration":[72],"exhibit":[73],"apparent":[75],"loss":[76,116,121],"rhabdomeric":[78,124],"at":[80,144,156,221],"early":[81],"times":[82],"degenerative":[85,245],"process.":[86],"Using":[87],"different":[88],"immunological":[89],"agents,":[90],"genetic":[91],"backgrounds,":[92],"treatments,":[95],"we":[96],"have":[97],"found":[98,140,155],"binding":[101,159],"arrestin":[103,153,161],"masked":[106],"C-terminal":[108],"monoclonal":[109],"antibody":[110,170],"epitope":[111,171],"resulted":[113],"immunoreactivity.":[119],"The":[120,229],"immunoreactive":[123],"only":[126,180],"occurred":[127],"when":[128],"depleted":[131,189],"from":[132,190],"plasma":[134],"membrane":[135],"such":[136],"it":[138,193],"within":[141],"rhabdomere":[143],"stoichiometric":[145],"levels":[146],"CONCLUSIONS:":[149],"When":[150],"equal":[157],"levels,":[158],"results":[164],"masking":[167,178],"C-terminus":[174],"rhodopsin.":[176],"Since":[177],"can":[179,194],"occur":[181],"after":[182],"most":[183],"has":[187],"been":[188],"rhabdomere,":[192],"be":[195],"concluded":[196],"endocytosis-induced":[199],"degeneration,":[201],"much":[202],"is":[206,220],"localized":[207],"cell":[210,241],"body":[211],"small":[213],"puncta.":[214],"These":[215],"data":[216,230],"suggest":[217],"extremely":[222],"high":[223],"local":[224],"concentrations":[225],"cytoplasm.":[228],"discussed":[232],"context":[235],"a":[237],"model":[238],"apoptosis":[242],"disorders.":[246]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}