{"id":"https://openalex.org/W2419254520","doi":null,"title":"Development of a cell-based assay for monitoring hepatitis C virus ns3/4a protease activity.","display_name":"Development of a cell-based assay for monitoring hepatitis C virus ns3/4a protease activity.","publication_year":2008,"publication_date":"2008-01-01","ids":{"openalex":"https://openalex.org/W2419254520","mag":"2419254520","pmid":"https://pubmed.ncbi.nlm.nih.gov/18999887"},"language":"en","primary_location":{"id":"pmid:18999887","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18999887","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta virologica","raw_type":"Evaluation Study"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011936967","display_name":"Lei Yf","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210158926","display_name":"Xijing Hospital","ror":"https://ror.org/05cqe9350","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210158926"]},{"id":"https://openalex.org/I9916479","display_name":"Air Force Medical University","ror":"https://ror.org/00ms48f15","country_code":"CN","type":"education","lineage":["https://openalex.org/I9916479"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Y F Lei","raw_affiliation_strings":["Department of Microbiology, Fourth Medical Military University, and Department of Blood Transfusion, Xijing Hospital, Shaanzi, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Fourth Medical Military University, and Department of Blood Transfusion, Xijing Hospital, Shaanzi, PR China","institution_ids":["https://openalex.org/I4210158926","https://openalex.org/I9916479"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100777410","display_name":"Wei Hua Yin","orcid":"https://orcid.org/0000-0001-8862-3197","observed_orcids":["https://orcid.org/0000-0001-8862-3197"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W Yin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100706424","display_name":"Jian\u2010Rong Yang","orcid":"https://orcid.org/0000-0002-7807-9455","observed_orcids":["https://orcid.org/0000-0002-7807-9455"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Yang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024266326","display_name":"Lv X","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"X Lv","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043211698","display_name":"Wei Sh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S H Wei","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043960028","display_name":"An Qx","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Q X An","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113767343","display_name":"Hu Xb","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"X B Hu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5074641041","display_name":"Xu Zk","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Z K Xu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2172,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.69532197,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"52","issue":"3","first_page":"133","last_page":"41"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10151","display_name":"Hepatitis C virus research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10151","display_name":"Hepatitis C virus research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepatitis-c-virus","display_name":"hepatitis C virus","score":0.9700000286102295},{"id":"https://openalex.org/keywords/ns3-4a-protease","display_name":"NS3/4A protease","score":0.9980000257492065},{"id":"https://openalex.org/keywords/protease-assay","display_name":"protease activity assay","score":0.8939999938011169},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.7319999933242798},{"id":"https://openalex.org/keywords/protease-inhibitors","display_name":"protease inhibitors","score":0.5950000286102295},{"id":"https://openalex.org/keywords/antiviral-drug-screening","display_name":"antiviral drug screening","score":0.8939999938011169},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.7139999866485596},{"id":"https://openalex.org/keywords/mtt-assay","display_name":"MTT assay","score":0.7770000100135803},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.5299999713897705}],"concepts":[{"id":"https://openalex.org/C132379496","wikidata":"https://www.wikidata.org/wiki/Q83141645","display_name":"NS3","level":4,"score":0.9420028328895569},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.8942900896072388},{"id":"https://openalex.org/C83100098","wikidata":"https://www.wikidata.org/wiki/Q106297070","display_name":"NS2-3 protease","level":4,"score":0.6867759823799133},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.472996324300766},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4580816626548767},{"id":"https://openalex.org/C82553315","wikidata":"https://www.wikidata.org/wiki/Q14905312","display_name":"MASP1","level":5,"score":0.4269990921020508},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4178459942340851},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39072805643081665},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35021716356277466},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3248542249202728},{"id":"https://openalex.org/C2777807008","wikidata":"https://www.wikidata.org/wiki/Q87324999","display_name":"Serine protease","level":4,"score":0.11893731355667114}],"mesh":[{"descriptor_ui":"D000085242","descriptor_name":"Viral Proteases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000998","descriptor_name":"Antiviral Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003124","descriptor_name":"Colorimetry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008826","descriptor_name":"Microbial Sensitivity Tests","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011480","descriptor_name":"Protease Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012697","descriptor_name":"Serine Endopeptidases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016174","descriptor_name":"Hepacivirus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D016174","descriptor_name":"Hepacivirus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017361","descriptor_name":"Viral Nonstructural Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D043583","descriptor_name":"Nucleoside-Triphosphatase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D047908","descriptor_name":"Intracellular Signaling Peptides and Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053148","descriptor_name":"Caspase 3","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D053148","descriptor_name":"Caspase 3","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D053487","descriptor_name":"DEAD-box RNA Helicases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:18999887","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18999887","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta virologica","raw_type":"Evaluation Study"},{"id":"mag:2419254520","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18999887","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8048999905586243}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2075952325","https://openalex.org/W2010156396","https://openalex.org/W2970149113","https://openalex.org/W3031040395","https://openalex.org/W2012797613","https://openalex.org/W2026645462","https://openalex.org/W4390469485","https://openalex.org/W1964481619","https://openalex.org/W1994876639","https://openalex.org/W75722855"],"abstract_inverted_index":{"Hepatitis":[0],"C":[1],"virus":[2],"(HCV)":[3],"NS3/4A":[4,40,71,88,100,122,132,163,171],"(non-structural":[5],"3":[6,62,80],"and":[7,21,102,127,157],"4":[8],"B)":[9],"protease":[10,41,101,123,133,164,172],"plays":[11],"a":[12,33,139,155],"key":[13],"role":[14],"in":[15,43,51,77,104,115,150],"the":[16,39,57,67,78,111,119,128,167],"processing":[17],"of":[18,38,53,92,107,148,169],"polyprotein":[19],"precursor":[20],"it":[22],"becomes":[23],"an":[24,48,105],"attractive":[25],"target":[26],"for":[27,36,70,87,161],"antiviral":[28],"drug":[29],"discovery.":[30],"We":[31],"developed":[32,154],"cell-based":[34],"assay":[35,160],"monitoring":[37],"activity":[42,165],"mammalian":[44],"cells":[45,109,120,149],"that":[46],"is":[47],"important":[49],"step":[50],"screening":[52,168],"specific":[54,68,86,97],"drugs":[55],"against":[56],"protease.":[58,72,89,112],"The":[59,73,90,113],"recombinant":[60],"caspase":[61],"(rCasp3)":[63],"was":[64],"used":[65],"as":[66],"substrate":[69],"endogenous":[74],"cleavage":[75,98],"sites":[76],"procaspase":[79],"molecule":[81],"were":[82],"substituted":[83],"by":[84,99,138],"decapeptides":[85],"activation":[91],"rCasp3":[93,126],"depended":[94],"on":[95,142],"its":[96],"resulted":[103],"apoptosis":[106],"stable":[108],"expressing":[110,121],"difference":[114],"cell":[116],"viability":[117],"between":[118],"transfected":[124],"with":[125,131],"counterparts":[129],"pretreated":[130],"inhibitors":[134],"could":[135],"be":[136],"estimated":[137],"spectrophotometry":[140],"based":[141],"3-(4,5-dimethylthioazol-":[143],"2-yl)-2,5-diphenyl-tetrazolium":[144],"bromide":[145],"(MTT)":[146],"staining":[147],"microplates.":[151],"Thus,":[152],"we":[153],"simple":[156],"cost-effective":[158],"colorimetric":[159],"evaluating":[162],"enabling":[166],"candidate":[170],"inhibitors.":[173]},"counts_by_year":[{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}